• Title/Summary/Keyword: Heating Condition

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Selection of Protease Hyperproducing Mutant Strain from Serratia marcescens ATCC 2 1074 and Enzymatic Properties of the Protease (Serratia macescens ATCC 21074로부터 Protease 생산성이 높은 변이주의 선별 및 Protease의 효소학적 특성)

  • 김홍립;오평수
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.450-455
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    • 1991
  • A protease hyperproducer, ampicillin resistant mutant, Serratia sp. SMNT-1 was selected from Serratia marcescens ATCC 21074 by mutagenesis. The protease productivity of this strain was about 11 times as much as that of the parental strain. The enzyme showed maximal activity at pH 9.0 and $40^{\circ}C$ and was stable over the pH range from 6.0 to 10.0 at $4^{\circ}C$, whereas it was unstable at $37^{\circ}C$ in alkaline condition. the enzyme was inactivated by heating at $60^{\circ}C$ for 10 min. The enzyme was inactivated by EDTA and reactivated by $Zn^{2+}, Co^{2+},\; and \; Mn^{2+}$, but the proteoiytic activity of the enzyme was not affected by DFP. From the above results, the protease produced by Serratia sp. SMNT-1 was classified as a metalloprotese.

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Experimental analysis of pultrusion process for phenolic foam composites (발포 복합재료 Pultrusion 공정의 실험적 해석)

  • Lee WooIl;Yun MyungSeok
    • Composites Research
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    • v.18 no.3
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    • pp.47-52
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    • 2005
  • Pultrusion process of phenolic foam composite is investigated. Phenolic foam composites provide heat and flame resistance with less weight. When made into foam, a variety of properties can be obtained with different bubble size and number density. In this study, effect of process variables on the foaming characteristics of phenolic resin composites during pultrusion process has been studied experimentally. The process variables considered are the heating temperature and the pulling speed as well as the mass fraction of blowing agent. Experiments were performed using a laboratory scale pultrusion apparatus. Optimal process condition was found by observing the micro-morphology.

Preparation of Mook with Sea Mustard and Sea Tangle 1. The Optimum Condition of Sea Mustard and Sea Tangle Mooks (미역과 다시마를 주원료로 한 묵 제조1. 미역, 다시마묵의 최적 조건과 그 물성에 관하여)

  • 정용현;김건배;최선남;강영주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.1
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    • pp.156-163
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    • 1994
  • To investigate the optimum conditions of seaweed Mooks prepared with sea mustard (Indaria pinnatifida) and sea tangel(Laminaria japonica), pH, viscosity , yield and jelly strength were studied on the sol and /or gel (Mook ) made from homogenized seaweeds. Solubilization conditions of homogenized seaweeds were heating at $65^{\circ}C$ for one hour after mixing homogenized seaweed with 1 % $K_2$HPO$_4$ by 1 ; 1 (v/v) . Gelation conditions were 24 hrs by natural permeation of Ca++ into the sol in three times (v/v) of 1 % CaCl$_2$ solution to solubilized seaweed passed through 60 mesh of sieve. There are generally no relationship between viscosity of solubilized seaweed and jelly strength of Mook produced by gelation of the sol in CaCl$_2$ solution . Jelly strength of sea mustard Mook was more than 500g/$\textrm{cm}^2$ , yield was 87.5% of the solubilized and filtrated seaweed . Jelly strength and specific gravity of sea tangle Mook were higher those of sea mustard Mook, while its yield was lower than that of sea mustard Mook.

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Culturability of Clostridium botulinum Spores under Different Germination Conditions, Sublethal Heat Treatments, and in the Presence of Nisin

  • Chung, Yoon-Kyung;Yousef, Ahmed E.
    • Preventive Nutrition and Food Science
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    • v.12 no.4
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    • pp.251-258
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    • 2007
  • Thermal resistance and heat activation characteristics of Clostridium botulinum ATCC 25763 spores were evaluated. The effects of nisin and pH on the activation and subsequent germination were also investigated. Spores of C. botulinum were not inactivated by heat treatments up to $92^{\circ}C$ for 2 hr. Heat treatment at $85^{\circ}C$ for 90 min was selected as the optimal activation condition based on monitoring subsequent germination. L-alanine alone or in combination with L-cysteine was not sufficient to germinate the spores of this strain. Tryptone-Peptone- Glucose-Yeast extract (TPGY) broth supplemented with L-alanine was used as a suitable germination medium. Decreasing pH of activation suspension increased the degree of phase darkening, i.e., germination. In addition, the presence of nisin during activation increased the degree of phase darkening. The majority of spore populations were dormant at a pH of less than 2.8, and these populations required heat activation to increase the culturability on TPGY agar medium. However, extended heating in the presence of nisin at pH 2.8 decreased the spore count; however, heat activation was less necessary at pH 3.4, compared at pH 2.8.

Heat Transfer and Flow Characteristics by Trapezoid Rod Array in Impinging Jet System (충돌제트계에서 사다리형 로드 배열에 의한 열전달 및 유동특성)

  • 금성민
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.13 no.9
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    • pp.904-913
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    • 2001
  • The objective of this study was to investigate the characteristics of jet flow and heat transfer caused by trapezoid rods array in impinging jet system. In this study, trapezoid rods have been set up in front of flat plate to serve as a turbulence promoter. The bottom width of trapezoid rod was W=4, 8 mm and oblique angle were 80$^{\circ}$. The space from rods to the heating surface was C=1, 2, 4 mm, the pitch between each rods was P=30, 40, 50 mm, and the distance from nozzle exit to flat plate was H=100, 500 mm. This results were compared with the case without trapezoid rods. As a result, when rods are installed in front of the impinging plate, the acceleration of the jet flow and the eddies due to the rods seem to contribute to the heat transfer enhancement. Among test conditions, the heat transfer performance was best for the condition of W=8 mm, C=1 mm, P=30 mm and H/B=10. The maximum heat transfer rate is about 1.9 times larger than that without trapezoid rods.

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Thermal Characteristics of Polyvinylchloride in Combustion Reaction Using TGA (TGA를 이용한 폴리염화비닐의 연소반응에서의 열적 특성 연구)

  • Seo, Su-Eun;Kang, Yun-Jin;Min, Cho-Young;Bae, Dong-Chul;Kang, Kyung-Sik
    • Journal of the Korea Safety Management & Science
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    • v.11 no.3
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    • pp.217-226
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    • 2009
  • The combustion reaction of polyvinylchloride(PVC) was investigated using a thermogravimetric technique under an air atmosphere condition at several heating rates from 10 to $50^{\circ}C$/min. To obtain information on the kinetic parameters, the dynamic thermogravimetric analysis curve and its derivative were analyzed by a variety of analytical methods such as Kissinger, Friedman, Chatterjee-Conrad, Ozawa and Coats-Redfern methods. The combustion reaction of PVC proceeded in two steps; the first step was caused by the dehydrochlorination process in PVC, and the second step by the combustion of polyene. The comparative works for the kinetic results obtained from various methods should be performed to determine the kinetic parameters, because there are tremendous differences in the calculated kinetic parameters depending upon the mathematical method taken in the analysis.

Analysis of Inner Temperature in High Strength Concrete under Standard Temperature-time Curve (표준화재곡선에 의한 고강도 콘크리트 부재의 내부온도 예측)

  • Song, Hun;Lee, Sea-Hyun;Mun, Kyung-Ju;Do, Jeong-Yun;Soh, Yang-Seob
    • Proceedings of the Korea Concrete Institute Conference
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    • 2005.05b
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    • pp.469-472
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    • 2005
  • With all ensuring the fire resistance structure as a method of setting the required cover thickness to fire, the RC is significantly affected from the standpoint of its structural stability that the compressive strength and elastic modulus is reduced by fire. Normally, the degradation of concrete member exposed to fire is largely dependent on the fire scale and fire condition. There is therefore a need to precisely predict the deterioration and fire damage of the exposed member. Thus, this work estimated the temperature distribution inside a member taking into consideration of the thermal properties by means of finite element method(FEM). The estimation results in a little higher prediction value than the experimental value in surface layer and is almost coincident with the experiment as the heating depth increase. From this work it can be known that the simulation application of FEM using the thermal properties of concrete member in high temperature gives rise to the confident prediction in the prediction of temperature distribution.

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Powder Synthesis and Sintering Behavior of Hydroxyapatite by Citrate Method (Citrate법을 이용한 수산화아파타이트 분말합성 및 소결특성)

  • 임병일;최세영;정형진;정형진;오영제
    • Journal of the Korean Ceramic Society
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    • v.33 no.9
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    • pp.1003-1011
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    • 1996
  • Hydroxyapatite powder was synthesized by a citrate method, . Char-like precursor composed of Ca8(HPO4)2(PO4)4.5H2O (OCP) and CaCo3 was found via viscous resin-like intermediate by heating the mixed aqueous solution of Ca(NO3)2.4H2O(NH4)2HPO4 and citric acid. Resulted powder was transformed into hydroxyapatite phase by firing over 120$0^{\circ}C$-135$0^{\circ}C$ for 4 hr using the powder calcined at 90$0^{\circ}C$ for 10 hr composed of mostly single hydroxyapatite phase. The sintered densities increased with firing temperature up to 130$0^{\circ}C$ but the highest relative density was about 94% of theoritical value. indicating the presence of closed pores. The maximum 96 MPa of flexural strength was obtained at 120$0^{\circ}C$ firing but the flexural strength showed lower values over the above sintering condition. Vitro test was performed by immersing of two jointed specimens in SBF for seven days and adhesion was observed between two specimens.

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Preparation of Nano-sized Titanium Oxide Powder Using Natural Polymer Matrix (천연고분자 매트릭스를 사용한 산화티탄 나노입자의 합성)

  • Kim, Soo-Jong;Han, Cheong-Hwa;Shim, Jae-Ho
    • Journal of the Korean Ceramic Society
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    • v.50 no.6
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    • pp.489-494
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    • 2013
  • Nano-sized titanium oxide powders were synthesized by a polymer matrix technique using pulp and Titanium tetraisopropoxide (TTIP) as starting materials. The synthesized powders were characterized by XRD and FE-SEM. The particle size of the powders was controlled by preparation conditions, such as heat treatment temperature and time. After investigating various drying and heat treatment conditions, 50-100 nm sized homogeneous titanium oxide particles were obtained by treating at $600^{\circ}C$ for 1 h. The crystallization and rapid growth of particles was accelerated by increasing heat treatment temperature and time. Anatase phase generated below $600^{\circ}C$ transformed to the rutile phase with increasing heat treatment temperature. Moreover, above $800^{\circ}C$, heat treatment time had a very large influence on particle growth, and changing the heating condition also had a large influence on crystal growth.

A Study on the Making of Slag Cement Clinker from Reduced and Modified Converter Slag (개질전로슬래그를 활용한 슬래그 시멘트 클링커 소성에 관한 연구)

  • Park Sun-Ku;Kim Young-Whan;Ko In-Yong
    • Resources Recycling
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    • v.11 no.6
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    • pp.24-30
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    • 2002
  • Reduced and modified converter slag was ball milled and sieved to -200/+325 mesh. CaO,$SiO_2$, $Fe_2$$O_3$ was added to slag powder and mixed to make it similar to the composition of normal portlant cement. The pellet made of this powder was heated from $1250^{\circ}C$ to $1450^{\circ}C$ for 15 min~45 min. Most feasible condition for making slag cement clinker is the heating more than 20 min at $1450^{\circ}C$. The compressive strength of the mortar made of this slag cement clinker was better than that of normal port-lant cement in long time curing.