• Journal of Food Hygiene and Safety
• /
• v.18 no.1
• /
• pp.6-13
• /
• 2003

Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.

• Tribology and Lubricants
• /
• v.30 no.2
• /
• pp.124-129
• /
• 2014

The piping system accounts for a large portion of the machinery structure of a plant, and is considered as a very important mechanical structure for plant safety. Accordingly, it is used in most energy plants in the nuclear, gas, and heavy chemical industries. In particular, the piping system for a nuclear plant is generally complicated and uses the reactor and its cooling system. The piping equipment is exposed to diverse loads such as weight, temperature, pressure, and seismic load from pipes and fluids, and is used to transfer steam, oil, and gas. In ultrasound infrared thermography, which is an active thermography technology, a 15-100 kHz ultrasound wave is applied to the subject, and the resulting heat from the defective parts is measured using a thermography camera. Because this technique can inspect a large area simultaneously and detect defects such as cracks and delamination in real time, it is used to detect defects in the new and renewable energy, car, and aerospace industries, and recently, in piping defect detection. In this study, ultrasound infrared thermography is used to detect information for the diagnosis of nuclear equipment and structures. Test specimens are prepared with piping materials for nuclear plants, and the optimally designed ultrasound horn and ultrasound vibration system is used to determine damages on nuclear plant piping and detect defects. Additionally, the detected images are used to improve the reliability of the surface and internal defect detection for nuclear piping materials, and their field applicability and reliability is verified.

• Fire Science and Engineering
• /
• v.28 no.6
• /
• pp.69-75
• /
• 2014

Recently Amore pacific rack-type warehouse fire broke out and argue an urgent improvement of fire protection design code including automatic sprinkler and detection design. Various type of commodities have their unique fire characteristics from fire spread rate and heat lease rate and fire hazard depends on storage height, rack arrangement, aisle width, fire load etc. With increasing ceiling height for more storage space prevent effective water spray of sprinkler head, also delays detection time causes failure of early suppression. To achieve fire protection code performance of this occupancy, Major code articles relating to a classification of commodity, sprinkler system installation, detection and fire fighting are reviewed and suggested based on fire case analysis, code review between country and field survey.

• Korean Journal of Veterinary Research
• /
• v.34 no.1
• /
• pp.115-125
• /
• 1994

In this study, we try to establish the rapid and specific detection system for Salmonella species. The PhoE gene of Salmonella species was amplified with two specific primers, ST5 and ST8c, using PCR. The probe prepared from the amplified PhoE gene was sequenced and applied for Southern blot analysis. After PCR with ST5 and ST8c primers for PhoE gene, DNA bands of expected size(365bp) from 7 different Salmonella species were detected, but not from 12 enterobacteriaceae and 3 gram positive bacteria. PCR was highly sensitive to detect up to 10fg of purified DNA template and to identify Salmonella species with only 320 heat-lysed bacterial cells. The inhibition of PCR amplification from stool specimen was occurred with 50-fold dilution but disappeared over 100 fold dilution of samples. It was confirmed that the PhoE genes were amplified and cloned with over 97% nacleotide sequence homology of PCR products compared with that of S. typhfmurium LT2. The DNA probe derived from S. typhimurium TA 3,000 showed highly specific and sensitive reaction with PCR products of all tested Salmonella species. These results indicate that PCR was rapid and sensitive detection method for Salmonella species and DNA probe prepared from S. typhimurium TA 3,000 was specific to identify PCR products of different Salmonella species.

• Journal of Veterinary Clinics
• /
• v.27 no.1
• /
• pp.23-28
• /
• 2010

Mycobacterium avium ssp paratuberculosis, intracellular bacteria that can cause chronic granulomatous enteritis in cattle, continues to pose significant economic losses and health problem with high prevalence. The purpose of this study is the polymerase chain reaction (PCR)-base strategy for early detection of M. avium ssp paratuberculosis in whole blood. Blood samples were collected from korean cattles in Jeonbuk, Korea. The 16 out of 88 serum samples were detected M. partuberculosis by ELISA. Then samples of infected 8 Korean cattles were amplified by PCR. The PCR amplified targets are 16s rDNA and heat shock protein 65kDa (hsp 65). The 16s rDNA provided a highly sensitive and specific tool for the direct detection of mycobacteria. In addition M. avium was confirmed characteristically by the hsp65. Finally there were sure to M. avium ssp paratuberculosis by IS900 PCR. The restriction fragment length polymorphism was identified by PCR amplifications and subsequence restriction enzyme digestions with Pst I of a hsp65. These results indicate that confirm M. avium with 16s rDNA, hsp65 and a restriction fragment length polymorphism in the hsp65 gene can be seem the other pattern. Therefore, these results can be used for clinical direct detections of M. avium ssp paratuberculosis in whole blood of Korean cattle and also to be used epidemiological researches.

• The Journal of the Korea institute of electronic communication sciences
• /
• v.12 no.6
• /
• pp.1219-1226
• /
• 2017

In this paper, it is performed the optimal design of the UV LED bar which can be used variously. The UV LED Bar emits ultraviolet rays, so it is important to emit ultraviolet rays constantly for the purpose of use. In order to emit a certain amount of ultraviolet rays as ever, the ultraviolet ray emission should be driven by a constant current source within the operable input voltage range. And also the heat dissipation is particularly important because of the long ultraviolet emission retention time due to the UV utilization characteristics. In addition, since human body protection is essential, the algorithm is configured to operate according to human body detection using distance sensor and Bluetooth. Three 365nm UV LEDs were used in series to emit ultraviolet UVA, operating at the constant current of 500mA with an efficiency of 87.5% and a power consumption of 6.006W. The ultraviolet radiation dose was measured at $5.35mW/cm^2$ at the distance of 10 cm when measured by the Lutron ultraviolet measuring instruments.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.10 no.8
• /
• pp.1810-1816
• /
• 2009

In 2007, 9,128 fires were actually caused by electrical faults and these fires resulted in 29 deaths and 262 injuries. Arc-faults were one of the major causes of these fires. When an unintended arc-fault occurs, it generates intense heat that can easily ignite surrounding combustibles. But, because conventional circuit breakers only respond to overloads, short circuits, and leakage currents, the breakers do not protect against arcing conditions. This paper presents results obtained in experiments on ignition behavior of wire by series arc fault currents and techniques developed to detect the arc-faults. The developed technique was tested after installation to make sure that they are working properly and protecting the circuit. If the developed arc fault detection technique is applied, the electrical fires caused by an arc-fault can be reduced.

• Journal of Convergence for Information Technology
• /
• v.11 no.5
• /
• pp.1-8
• /
• 2021

In this paper, we propose the construction of an IOT that monitors foresters' physical abnormalities in real time, performs emergency measures, and provides alarms for natural disasters or heatstroke such as a nearby forest fire or landslide. Nodes provided to foresters include 6-axis sensors, temperature sensors, GPS, and LoRa, and transmit the measured data to the network server through the gateway using LoRa communication. The network server uses 6-axis sensor data to determine whether or not a forester has any signs of abnormal body, and performs emergency measures by tracking GPS location. After analyzing the temperature data, it provides an alarm when there is a possibility of heat stroke or when a forest fire or landslide occurs in the vicinity. In this paper, it was confirmed that the real-time detection of physical abnormalities of foresters and the establishment of disaster early warning IOT is possible by analyzing the data obtained by constructing a node and a gateway and constructing a network server.

• Journal of Microbiology and Biotechnology
• /
• v.33 no.9
• /
• pp.1170-1178
• /
• 2023

Food allergy represents a severe problem for many societies, including sensitive populations, academies, health authorities, and the food industry. Peanut allergy occupies a special place in the food allergy spectrum. To prevent consumption by consumers suffering from a peanut allergy, a rapid and sensitive detection method is essential to identify unintended peanut adulteration in processed foods. In this study, we produced four monoclonal antibodies (MAbs; RO 3A1-12, PB 4C12-10, PB 5F9-23, and PB 6G4-30) specific to thermo-stable and soluble proteins (TSSPs) of peanut and developed an enzyme-linked immunosorbent assay (ELISA) based on the MAbs. Among them, PB 5F9-23 MAb was firmly bound to Ara h 1, and other MAbs strongly reacted to Ara h 3 in the Western blot analysis. An antibody cocktail solution of the MAbs was used to enhance the sensitivity of an indirect ELISA, and the limit of detection of the indirect ELISA based on the antibody cocktail solution was 1 ng/ml and improved compared to the indirect ELISA based on the single MAb (11 ng/ml). The cross-reaction analysis revealed the high specificity of developed MAbs to peanut TSSPs without cross-reaction to other food allergens, including nuts. Subsequently, analyzing processed foods by indirect ELISA, all foods labeled as containing peanuts in the product description were confirmed to be positive. The results indicate that the developed antibodies exhibit high specificity and sensitivity to peanuts and can be used as bio-receptors in immunoassays or biosensors to detect intentional or unintentional adulteration of peanuts in processed foods, particularly heat-processed foods.

• Korean Journal of Remote Sensing
• /
• v.39 no.5_4
• /
• pp.1145-1153
• /
• 2023

Urban trees play an important role in absorbing carbon dioxide from the atmosphere, improving air quality, mitigating the urban heat island effect, and providing ecosystem services. To effectively manage and conserve urban trees, accurate spatial information on their location, condition, species, and population is needed. In this study, we propose an algorithm that uses a high-resolution urban tree cover map constructed from deep learning approach to separate trees from the urban land surface and accurately detect tree locations through local maximum filtering. Instead of using a uniform filter size, we improved the tree detection performance by selecting the appropriate filter size according to the tree height in consideration of various urban growth environments. The research output, the location and height of individual trees in human settlement over Suwon, will serve as a basis for sustainable management of urban ecosystems and carbon reduction measures.