• Clinical and Experimental Pediatrics
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• 제54권8호
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• pp.335-339
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• 2011

Purpose: Hyperhomocysteinemia is known as a risk factor for atherosclerosis. Preclinical arteriosclerosis is noted and premature atherosclerosis is known to be accelerated in Kawasaki disease (KD) patients. Genetic polymorphisms in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene result in elevated plasma homocysteine concentrations and are known to be associated with the development of coronary artery disease. Our hypothesis is that single nucleotide polymorphisms (SNPs) of the MTHFR gene are related to the development of KD and coronary artery lesions (CALs). Methods: For this study, we selected 3 candidate single nucleotide polymorphisms (SNPs) (rs2274976, rs1801131, and rs1801133) of MTHFR. These SNPs are located on chromosome 1p36.3. We included 101 KD patients and 306 healthy adults as controls in this study. CALs were seen in 38 patients. Genotypes of the selected SNPs were determined by direct sequencing and analyzed with SNPAlyze. Results: The genetic distribution and allelic frequency of the 3 MTHFR SNPs (rs2274976, rs1801131, and rs1801133) were not significantly different in patients with KD compared to the control group (P=0.71, 0.17, and 0.96, respectively). There was no difference in the genetic distribution of the MTHFR SNPs between the normal control group and the CAL group (P=0.43, 0.39, 0.52 respectively). Conclusion: The genetic distribution of the MTHFR SNPs (rs2274976, rs1801131, and rs1801133) was not different in the KD group compared to the control group. In addition, the genetic distribution of these SNPs was not different in the CAL group compared to the control group in the Korean population.

• 농촌의학ㆍ지역보건
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• 제25권2호
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• pp.315-326
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• 2000

본 연구는 농촌지역 청소년들의 건강 행태와 최근 청소년들에게 중요한 문제가 되고있는 성 문제, 약물 남용, 청소년 폭력과 정신 보건 관련 문제를 전반적으로 파악하고 이들간의 관련성을 분석하고자 시도되었다. 본 연구에서 얻은 연구 결과를 요약하면 다음과 같다. 첫째, 조사 대상 학생들의 대부분이 자신의 건강상태를 건강하다고 인지하고 있었으나 건강에 대한 관심도는 70% 정도만이 관심을 가지고 있었다. 둘째, 대상 학생들의 건강행태와 비행행태 조사결과 타지역에 비하여 성경험률, 약물 사용률은 낮았으나 흡연률, 음주율은 오히려 높았으며 수면 시간도 부족한 것으로 나타났다. 셋째, 건강관련 행태와의 관련 요인을 분석한 결과 인구 사회학적 특성 중에서는 남자인 경우에서, 중학생에서, 용돈이 적은 경우와 성적이 높은 경우에서 건강행태 실천률이 높았고 건강에 대한 관심이 높을수록, 자살시도를 한 경우보다는 자살 시도 경험이 없는 경우에서, 중학생에서, 건강행태가 양호하였다. 이러한 결과를 종합할 때 농촌 지역 청소년들이 다양한 건강관련 문제점에 직면하고 있음을 알 수 있다. 따라서 대상지역 학생들의 다양한 건강관련 문제점을 파악하고 이를 바탕으로 사업의 우선순위를 선정하여 건강행태를 긍정적으로 개선하고 관리 해나자는 한편 건강에 대한 관심을 증대시키려는 노력이 더욱 활성화되어져야 하겠다.

• 대한화장품학회지
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• 제45권4호
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• pp.353-361
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• 2019

본 연구에서는 Fervidobacterium islandicum AW-1를 이용하여 케라틴 펩타이드를 생산하고, 두피 및 모발에 관련된 인자를 확인하여, 화장품 원료로서 케라틴 펩타이드의 가능성을 알아보았다. 모유두 세포주에 케라틴 펩타이드를 농도에 따라 세포 독성 및 증식을 살펴본 결과, 세포 독성은 나타나지 않았고, 세포증식에도 효과가 없었다. 케라틴 펩타이드를 함유한 헤어 샴푸, 헤어 에센스를 제조하여 인체 첩포 시험을 거쳐 무자극을 확인하였다. 30 명의 건강한 성인 피험자를 대상으로 4 주 동안 샴푸 및 에센스를 2 그룹으로 나누어 사용한 결과, 육안평가에서 윤기, 탈모, 두피 트러블, 푸석거림에서 통계적으로 유의한 긍정적 결과를 나타내었다. 두피 수분량은 샴푸와 에센스 사용 전 대비 2, 4 주 후에 통계적으로 유의한 증가를 나타내었다. 두피에 대한 경피수분손실양은 에센스를 사용한 그룹에서 사용 전 대비 4주 후에 통계적으로 유의한 감소를 나타내었다. 두피 피지량 역시, 에센스를 사용한 그룹에서 사용 전 대비 4 주 후에 통계적으로 유의한 감소를 나타내었다. 정상 모발, 손상 모발을 이용한 헤어 샴푸와 에센스의 사용 전후의 빗질에 의한 마찰력을 측정한 결과, 사용 전 대비 모두 통계적으로 유의한 변화를 나타내었다. 정상 모발의 경우, 사용 전보다 빗질의 마찰력이 증가하였고, 손상 모발의 경우 사용 전보다 빗질의 마찰력이 감소하였다. 이를 통해 케라틴 펩타이드는 모발 및 두피 관련한 제품에 사용할 수 있는 원료로 확인하였다.

• 대한바이러스학회지
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• 제27권2호
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• pp.161-168
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• 1997

The present study was designed to estimate the seroprevalence of NLVs among diarrheagenic children and in healthy adults in Seoul and its vicinity with the use of an EIA and an Western blot (WB) based on recombinant Norwalk virus capsid protein (rNV) and crude virus preparations as antigen. Seroconversion was observed in 34 (83%) of 41 tested using the EIA and in 21 (54%) of 39 using the WB, suggesting that the NLVs with epitopes common to rNV are prevalent in Seoul area. Diarrheal children who were known to have been infected with several other strains of the NLVs showed no significant antibody response to the rNV. Infection with rNV occurred earlier in life: primary infections with rNV were common before the age of 6 months and over 91 % of children had evidence of infection by that age by the EIA. Since the amount of the NLV antigens available for seroepidemiologic surveys is limited, we tried to detect NLV antibody by using crude virus preparations as antigen. One crude virus preparation of a child whose stool yielded genetically distinct NLV revealed the presence of the plural number of bands upon SDS-PAGE, but precipitated only one band (62 kDa) after the WB with a serum (collected 10 days after the onset of symptoms) of another diarrheal child. The WB assay we present in this report revealed that the NLVs are prevalent among Korean population and that the sera contained antibody to a single major structural protein, with molecular sizes of 58 to 62 kDa, compatible with the sizes reported for the Norwalk virus and Snow Mountain agent proteins, respectively. When the results of the WB were compared with those obtained by the EIA, the EIA antibody assay was sensitive enough to detect an antibody rise of as much as 4096-fold but not as specific as the WB. The WB assay presented in this paper will provide a powerful tool to elucidate not only antigenic structures of the NL Vs but also seroepidemiology of the NLV infection. The availability of an unlimited source of antigen will enable a large scale serologic studies that will greatly increase our understanding of the role of NLVs in human enteric illness.

• 한국운동역학회지
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• 제25권3호
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• pp.275-281
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• 2015

Objective : In order for Taekwondo athletes to perform destructive kicking performance, they are expected to have Taekwondo-specific muscle properties such as high muscle strength and power. The purpose of this study was to investigate the joint angle-dependent force-producing property of Taekwondo athletes' knee extensor muscles, which is one of the primary muscle groups involved in kicking performance. Method : Ten Taekwondo male athletes (age: $19.9{\pm}0.7yrs$, height: $180.6{\pm}6.2cm$, body mass: $75.9{\pm}8.9kg$, career: $9.2{\pm}2.9yrs$.) and 10 healthy male non-athletes (age: $26.3{\pm}2.6yrs$, height: $174.2{\pm}4.8cm$, body mass: $72.8{\pm}7.7kg$) participated in this study. Subjects performed maximum isometric knee extension at knee joint angles of $40^{\circ}$, $60^{\circ}$, $80^{\circ}$, and $100^{\circ}$ (the full knee extension was set to $0^{\circ}$) with the hip joint angles of $0^{\circ}$ and $80^{\circ}$ (the full extension was set to $0^{\circ}$). During the contractions, knee extension torque using an isokinetic dynamometer simultaneously with muscle activities of the rectus femoris (RF), and the vastus lateralis (VL) and vastus medialis (VM) using surface electromyography were recorded. Based on the torque values at systematically different knee-hip joint angles, the joint torque-angle relationships were established and then the optimal joint angle for the knee extensor was estimated. Results : The results of this study showed that the isometric knee extension torque values were greater for the Taekwondo athletes compared with the non-athlete group at all hip-knee joint angle combinations (p<.05). When the hip joint was set at $80^{\circ}$, the peak isometric torque was greater for the Taekwondo athletes compared with the non-athlete group ($313.61{\pm}36.79Nm$ and $221.43{\pm}35.92Nm$, respectively; p<.05) but the estimated optimum knee joint angles were similar ($62.33{\pm}5.71^{\circ}$ and $62.30{\pm}4.67^{\circ}$ for the Taekwondo athletes and non-athlete group, respectively). When the hip joint was set at $0^{\circ}$, the peak isometric torque was greater for the Taekwondo athletes compared with the non-athlete group ($296.29{\pm}45.13Nm$ and $199.58{\pm}25.23Nm$, respectively; p<.05) and the estimated optimum knee joint angle was larger for the Taekwondo athletes compared with the non-athlete group ($78.47{\pm}5.14^{\circ}$ and $67.54{\pm}5.77^{\circ}$, respectively; p<.05). Conclusion : The results of this study suggests that, compared with non-athletes, Taekwondo athletes have stronger knee extensor strength at all hip-knee joint angle combinations as well as longer optimum muscle length, which might be optimized for the event-specific required performance through prolonged training period.

• 대한임상전기생리학회지
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• 제10권2호
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• pp.7-14
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• 2012

Purpose : This study investigated the validity of crosswalk signal time length with regards to elderly stroke patients. Methods : We recruited 60 elderly adults sixty years of age or older for our study. The participants were divided into three groups.Group A consisted of 20 healthy participants with no walking aids. Group B consisted of 20 stroke patients with no walking aids. Group C consisted of 20 stroke patients using a cane as a walking aid. We measured the walking times of participants for 7 m, 14 m and 21 m lengths. Results : Using an independent t test, there was a statistically significant difference in the walking times between Group A and Group B for all lengths. There was a statistically significant difference in the walking times between Group A and Group C for all lengths. There was no statistically significant difference in the walking times between Group B and Group C. There was a statistically significant difference between the three group when using ANOVA. Conclusion : From the results of this study, we infer that the signal times at crosswalks are inappropriate for elderly stroke participants who use a cane as a walking aid. Therefore further research should be conducted to determine the appropriate amount of additional time needed for the elderly to safely cross the street.

• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1798-1807
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• 2002

This study was conducted to investigate lactobacillus salivarius subsp. salivarius having probiotic properties to be used as the health adjuncts with fermented milk products. Acid- and bile-tolerant lactobacillus salivarius subsp. salivarius was isolated with lactobacilli MRS broth from faeces of 80 healthy persons (infants, children and adults). It was used as a probiotic strain in fermented milk products. The pH of fermented milk decreased from pH 6.7 to 5.0 and titratable acidity increased from 0.3% to 1.0% by L. salivarius subsp. salivarius (isolation strain 20, 35, and 37), when incubated for 36 h at 37$^{\circ}C$. The number of viable cell counts of fermented milk was maximized at this incubation condition. The SDS-PAGE evidenced no significant change of casein but distinct changes of whey protein were observed by isolated L. salivarius subsp. salivarius for titratable acidity being incubated by 0.9-1.0% at 37$^{\circ}C$. All of the strains produced 83.43 to 131.96 mM of lactic acid and 5.39 to 26.85 mM of isobutyric acid in fermented products. The in vitro culture experiment was performed to evaluate ability to reduce cholesterol levels and antimicrobial activity in the growth medium. The selected L. salivarius subsp. salivarius reduced 23-38% of cholesterol content in lactobacilli MRS broth during bacterial growth for 24 h at 37$^{\circ}C$. All of the isolated L. salivarius subsp. salivarius had an excellent antibacterial activity with 15-25 mm of inhibition zone to E. coli KCTC1039, S. enteritidis KCCM3313, S. typhimurium M-15, and S. typhimurium KCCM40253 when its pH had not been adjusted. Also, all of the isolated L. salivarius subsp. salivarius had partial inhibition zone to E. coli KCTC1039, E. coli KCTC0115 and S. enteritidis KCCM3313 when it had been adjusted to pH 5.7. The selected strains were determined to have resistances of twelve antibiotic. Strains 27 and 35 among the L. salivarius subsp. salivarius showed the highest resistance to the antibiotics. These results indicated that some of the L. salivarius subsp. salivarius (strain 27 and 35) are considered as effective probiotic strains with a potential for industrial applications, but the further study is needed to establish their use as probiotics in vivo.

• 한국콘텐츠학회논문지
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• 제13권11호
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• pp.791-799
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• 2013

본 연구는 키네시오 테이핑을 적용하는 방식에 따라 나타나는 척수운동신경원의 흥분성과 뇌파의 변화를 알아보기 위해 실시하였다. 건강한 성인 16명을 대상으로 비복근의 테이핑 적용 방식에 따라 실험군 I(n=8); 근육 주행방향, 실험군 II(n=8); 근육 횡 방향으로 구분하여 2주간 실시하였다. 척수운동신경원 흥분성 측정을 위해 경골신경에 자극을 주어 H 반사를 획득하였으며, 뇌파는 ${\beta}$-SMR를 측정하기 위해 C3, Cz, C4에 활성전극을 붙여 테이핑 적용 전, 즉시, 1주일 후, 2주일 후에 측정하였다. 연구 결과, 실험군I의 척수 운동신경원의 변화는 실험군 II보다 ${\alpha}$-운동신경원의 활성도가 감소하였고 지속 시간도 길었다(p<.05). 뇌파는 실험군I의 ${\beta}$-SMR 활성도가 실험군 II 보다 증가하고 지속시간도 길었다(p<.05). 근육 주행방향에 따른 적용 방식은 횡 방향 보다 ${\beta}$-SMR 뇌파를 더 활성화 시키며, 척수운동신경원의 활성도 감소를 지속적으로 유발함을 알 수 있었다.

• Journal of Nutrition and Health
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• 제47권4호
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• pp.229-235
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• 2014

본 연구는 자일로올리고당을 설탕에 적용하여 임상시험을 통해 자일로올리고당 첨가량에 따른 설탕의 GI 저감효과와 혈당저감효과를 확인하였다. 건강한 성인 남녀 11명를 대상으로 표준식품인 포도당과 비교하여 분말 자일로올리고당을 14% 함유한 Xylo 14과 분말 자일로올리고당을 20% 함유한 Xylo 20을 섭취 후 표준식품과 비교식품의 IAUC를 통해 GI를 산출한 결과 Xylo 14는 $60.0{\pm}23.5$로 Medium GI 식품으로 나타났으며, Xylo 20은 $54.3{\pm}17.7$로 Low GI 식품으로 나타났다. 순수한 설탕의 GI 68과 비교하면 각각 11.8%와 20.1% 혈당상승 저감효과를 나타낼 수 있었다. AUC는 15~90분까지 모든 구간에서 유의적인 차이 (p < 0.05)를 나타내었다. 따라서 Xylo 14와 Xylo 20은 혈당 저감효과를 나타냄과 동시에 7~10 g 이상 섭취하면 자일로올리고당의 1일 유효섭취량도 만족시킬 수 있으므로 기능성 설탕으로서의 활용이 기대된다.

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.174-182
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• 2004

The zinc intake and status of South Koreans from rural, urban and metropolitan areas were compared to evaluate the zinc nutritional status in different regional areas in South Korea. The dietary habits of 721 healthy adult subjects (271 from rural, 240 from urban, 210 from metropolitan city) with an age range 30 ∼ 64 (mean age 54$\pm$18) were assessed using a food frequency questionnaire. Mean daily Zn intake for rural, urban, and metropolitan areas was 6.5 mg, 7.3 mg, and 11.4 mg (p<0.05), respectively, which was 54%,61% and 95% of the Korean RDA for man (12 mg/d). Mean phytate: zinc molar ratios for rural, urban, and metropolitan city were 41, 34, and 30, respectively (p < 0.05), which were higher than the cutoff level of 20 for poor zinc status. The zinc intake and phytate: Zn molar ratio in the rural area were 0.5 and 1.3-fold compared to those of the metropolitan city, which can cause poor zinc nutriture in the rural area. Most of the zinc biomarkers were lower in the rural area than in the metropolitan city (p < 0.05) (mean rural and metropolitan values for plasma Zn: 80.8 $\mu\textrm{g}$/dL and 119.8 $\mu\textrm{g}$/dL, respectively; RBC Zn: 7.8 $\mu\textrm{g}$/dL and 8.8 $\mu\textrm{g}$/dL, respectively; plasma alkaline phosphatase (ALP) activity: 87 mU/mL and 100.4 mU/mL, respectively). It seems that a lower zinc intake in the rural area decreased zinc biomarker levels, such as plasma and RBC zinc, and plasma alkaline phosphatase activity, and caused the poor zinc nutritional status in this area. Most of the zinc biomarkers, such as RBC zinc and urinary and plasma zinc levels, in the subjects from the three localities, were within the normal range even when zinc intake of rural and urban subjects was low. The exception was plasma ALP activity in the rural area, which was lower than the reference level. Thus, marked zinc deficiency in these subjects were not observed, however, the potential for marginal zinc deficiency should be considered, especially for the rural area, because of the low zinc intake and the biomarker levels for marginal zinc deficiency.