• 지역사회간호학회지
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• 제27권4호
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• pp.410-422
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• 2016

Purpose: This study evaluated the effects of a cultural competence training program for public health nurses (PHNs) using intervention mapping. Methods: An embedded mixed method design was used. Forty-one PHNs (experimental: 21, control: 20) and forty marriage migrant women (MMW) (20, in each group) who were provided nursing care by PHN participated in the study. The experimental group was provided with a four-week cultural competence program consisting of an eight hour offline and online course, e-mail newsletters and social networking services (BAND). Transcultural Self-efficacy (TSE) of the PHNs, client-nurse trust, and satisfaction with nursing care of MMW were measured. Ten PHNs in the experimental group were interviewed after the experimental study. Results: The experimental group showed a significantly greater improvement in TSE, client-nurse trust, and satisfaction with nursing care than did the control group. Six themes emerged from qualitative data: (a) Recognizing cultural differences, (b) Being interested in the multicultural policy, (c) Trying to communicate in MMW's own language, (d) Providing medical information using internet and smart phone, (e) Embracing culturally diverse people into society, and (f) Requiring ongoing cultural competence training. Conclusion: Cultural competence training enabled PHNs to provide culturally competent care and contribute to MMW's health outcomes.

• 한국산업보건학회지
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• 제20권4호
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• pp.274-281
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• 2010

The objectives of this study are (a) to investigate the distribution patterns and exposure concentrations of biological agents in sawmill industries and (b) to compare sampling methods of biological agents. The representative processes of 5 sawmills were selected to measure total airborne bacteria, fungi, endotoxin as well as dust. Airborne bacteria and fungi were measured with one stage impactor, six stage impactor and gelatin filteration methods. Endotoxin was collected with polycarbonate filters and analysed by kinetic chromogenic Limulus Amebocyte Lysate method. Geometric mean levels of airborne bacteria, fungi, endotoxin and dust were 1,864 CFU/$m^3$, 2,252 CFU/$m^3$, 31.5 EU/$m^3$ and 2.4 mg/$m^3$. The ratios of indoor/outdoor concentrations were 3.7 for bacteria, 4.1 for fungi, 3.3 for endotoxin and 9.7 for dust. The respiratory fractions of bacteria were 68.0, 50.9, 49.2 and 45.1% in band-saw, table-saw, rip-saw process and outdoor air. The respiratory fractions of fungi were 78.7, 90.8, 87.5 and 84.8% in band-saw, table-saw, rip-saw process and outdoor air, respectively. There was no significant differences in bacterial concentrations among single stage, six stage impaction and filteration methods. But, fungal concentrations measured with filtration methods were significantly higher than those with impaction methods. Geometric mean levels of airborne bacteria and fungi were higher than the OSHA guideline values of 1,000 CFU/$m^3$. The respiratory fractions of fungi were above 75%. The concentrations of biological agents were significantly different among culture-based sampling methods. In the exposure assessments of biological agents, further studies are needed for the comparisons of diverse sampling methods and the investigations of environmental factors.

• 약학회지
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• 제48권6호
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• pp.384-390
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• 2004

Polyphenol oxidase-catalyzed oxidation of substrates (t-butylcatechol, 4-methylcatechol, chlorogenic acid, caffeic acid and pyrocatechol) were performed in the Ph range 4~8. Co ncentrations of substrate's major oxidation products were monitored by high performance liquid chromatograph. The nature and amounts of products formed were highly pH dependent. They also were ifluenced by kinds of substrates. Major oxidation product of 4-methylcatechol appeared the maxium value at pH 5, them of chlorogenic acid, caffeic acid and pyrocatechol at pH 6.0 and that of t-butylcatechol at pH 5~7. Time-dependent PPO activity was determined at $4^{\circ}C\;and\;30^{\circ}C$. PPO extracted by phosphate buffer containing triton X-114 (t-PPO) was more stable than PPO by phosphate buffer (b-PPO). The result of electrophoresis, at first PPO was showed only a band at 48 kd. After 1~3 days a partial degrade band was appeared in b-PPO and three partial degrade bands in t-PPO. No activity band was appeared in PPOs at $30^{\circ}C$ and b-PPO at $4^{\circ}C$ after 4 days. And a band (37 kDa) in t-PPO was remained finally and disappered. PPO from Perillae leaves has two activity bands at 48 and 37 kDa in previous paper. It was supposed that PPO in the leaves of Perilla frutescens was a protein having one molecular weight as 48 kDa. And 37 kDa protein, relatively proteolysis-resistant, was a proteolyzed form of a major form.

• 한국안광학회지
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• 제11권2호
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• pp.77-83
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• 2006

본 연구에서는 프리즘 처방을 위해 편심된 안경렌즈의 산각위치에 따른 수차와 경사각에 따른 수차를 계산하였다. 프리즘 처방을 위해서 일반적으로 일반 렌즈를 편심시켜 만든다. 광축과 주시축 사이의 차이는 수차를 발생시키기 때문에, 이런 경우 산각의 위치가 중요하다. 우리는 계산을 통하여 산각 회전 띠의 축이 (+)렌즈의 전면 곡률 중심을 지나는 것이 후면곡률 중심을 지나는 것보다 비점수차가 적게 발생하였고, 산각 회전 띠의 (-) 렌즈의 후면곡률 중심을 지나는 것이 전면보다 비점수차가 적게 발생한다는 결과를 얻었다. 렌즈의 굴절력이 클수록 비점수차가 작았다.

• 한국식품과학회지
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• 제29권3호
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• pp.595-600
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• 1997

양파껍질중에 존재하는 항산화물질을 여러 용매로 분획하여 그 항산화효과를 비교하고, butanol fraction의 TLC에 의한 재분획 및 총페놀함량, 수소공여능 및 UV spectral 분석을 행하였다. 메타놀추출물의 항산화 효과는 첨가농도가 증가함에 따라 증가되었다. 메타놀추출물의 용매 분획물의 항산화효과는 buthanol> ethyl acetate> ethyl ether>water fraction의 순으로 나타났다. 이들의 항산화효과는 총페놀함량 및 수소공여능과 밀접한 관계를 보였다. TLC에 의한 butanol fraction의 재분획 결과 Rf치 0.20, 0.33, 0.49, 0.60 및 0.94을 나타내는 형광띠를 나타내었다. Rf치 0.94의 형광 band는 다른 형광 band에 비해 현저히 강한 것으로 보여 주었으며 flavonol의 존재를 나타내는 에서 나타나는 255 nm와 317 nm에서 강한 흡수가 보였다. Spectral analyses 결과 양파껍질 중에 함유되어 있는 주요 항산화성 물질은 quercetin aglycone (3,3',4',5,7-pentahydroxyflavone)인 것으로 확인되었다.

• 대한물리치료과학회지
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• 제31권1호
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• pp.33-45
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• 2024

Background: This study aimed to investigate the effect of elastic band-resistive exercise using audio-visual medium on pain, proprioception, and motor function in adults with chronic neck and shoulder pain. Design: One group pretest-posttest follow-up experimental design. Method: Twenty adult women with neck and shoulder pain voluntarily participated in this study. Elastic band-resistive exercise using audio-visual medium including cervical flexion and extension, shoulder external rotation, and scapular retraction-protraction motions was conducted 5 times a week for 3 weeks. The Numerical Rating Scale, pressure threshold tool, CROM goniometer, and Image J software were used to assess subjective pain level, tenderness threshold (pain), joint position sense error (proprioception), joint range of motion, and postural alignment (motor function), respectively. Result:: The pain intensity and threshold and joint position sense error showed significant decreases after the intervention, whereas the joint range of motion angle revealed significant increases. The postural alignment including forward head posture and rounded shoulder revealed significant improvements after the intervention. Conclusions: Therefore, we suggest that elastic band-resistive exercise through audio-visual medium would be helpful in preventing and managing pain and physical dysfunction in individuals with chronic neck and shoulder pain, and then it would support the development of health management-related online education content.

• 농촌의학ㆍ지역보건
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• 제13권1호
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• pp.41-59
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• 1988

우리나라에서 문제가 되고 있는 몇몇 기생충질환을 대상으로 각종의 혈청학적 진단법을 적용시켜 진단적 가치 및 의의에 대하여 검토하였다. 연구대상 기생충은 간흡충, 낭미충, Capillaria hepatica이었으며, 주로 간접 형광항체반응, ELISA, western blot등의 기법을 적용시켜 보았다. 결과를 요약하면 다음과 같다. 1) 간흡충증에 있어서 ELISA는 83.3%의 민감도를 나타낸 반면 폐흡층 및 조충과의 교차반응이 인정되었다. ELISA는 간접혈구응집반응, 간접형광항체 반응에 비해 우수한 성적을 보이고 있었다. 앞서 기술한 교차반응을 구별하고 항원-항체반응의 특이항원대를 알아보기 위해 실시한 western blot의 결과 59Kd band와 21K의 band는 간흡충감염자 혈청이외에는 반응하지 않아 종특이성이 있는 것으로 판단되었다. Praziquantel로 치료한 다음 18개월 후에 혈청 및 뇨를 이용하여 ELISA로 검사한 결과 OD치는 치료전의 약 1/2수준으로 감소하였고, 음전률은 60%에 이르렀다. 간접혈구응집반응을 이용할 경우 치료 18개월 후 80%가 음전되었다. 2) 낭미충증 진단에 있어서 간접형광항체반응은 95.8%(23/24) 민감도를 나타내었으며 내막에서 가장 강한 반응을 나타내었다. ELISA 역시 90.0%(36/40)의 높은 민감도를 나타내었으나 두 방법 모두에서 다른 기생충감염자 혈청과의 교차반응이 인정되었다. Western blot 에서 볼때 91, 63, 21Kd의 band가 종특이한 것으로 나타났으며, 이중 63Kd의 항원대가 일관성 있게 낭미충 감염 혈청과 반응하였다. 3) Capillaria hepatica 충란을 이용한 난주위침강반응 및 간접형광항체법에서 85.0%의 민감도를 나타내었으며, 초록색의 특이한 형광이 점막전주위 및 충란절단면의 내막에서 관찰되었다. 수용성충란항원을 이용한 ELISA에 있어서도 85.0%의 민감도를 나타내었다. 항체가 감염후 3~5주부터 급격히 상승하기 시작하여 9주부터 점차 감소되어 감염후 13주에는 음성으로 전환되었다.

• 농촌의학ㆍ지역보건
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• 제16권1호
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• pp.79-89
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• 1991

A systematic study was conducted to identify and isolate a serologically pertinent antigen with high specific activity and low cross reactivity from Cysticercus parenchymal antigen. Differential centrifugation of the homogenate yield three particulate and one soluble fractions ; the $480{\times}G$ pellets($CyL_2$), the $7650{\times}G$ pellet($CyL_3$), the $100000{\times}G$ pellet($CyL_4$), and $100000{\times}G$ supernatant($CyL_6$). We compared antigenicity of these antigens to that or cystic fluid antigens($CyF_1$), saline extract of cystic wall($CyL_1$), and n-butanol treated $GyL_4$ antigen ($CyL_6$) based on SDS-PAGE and immunoblot techniques. The data obtained were as follows : 1) The ratio of O.D. value of ELISA against cysticercosis positive pool sera to that of negative pool sera was highest when using $CyF_1$ as antigen. However the ratio was relatively low in case of $CyL_{3.4}$ and $CyL_5$. 2) We have noted in previous paper that most strong antigenic activities are present in 63Kd band with low cross reactivities. An effective serologic reagent must contain components that are recognized by most infected sera. 63Kd band met this criteria and could be considered as a reliable band for the diagnosis of cysticercosis. As far as 63Kd band concern, $CyL_5$ showed most strong activities without disturbance of cross reaction by EITB in spite of low applicability to microplate ELISA. 3) $CyL_5$ could detect the serum antibody of cysticercosis even in very low titers, around cut-off values of microplate ELISA, by immunoblot. It also could detect the cross reactivities of Echinococcus species, which showed high absorbance value in micro plate ELISA and some sparganosis cases. Further purification of this antigen will be able to represents a antigen that can be used in the diagnosis of cysticercosis.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2005년도 ICCAS
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• pp.1826-1829
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• 2005

Recent advancement in wireless communications and electronics has enabled the development of low power sensor network. Wireless sensor network are often used in remote monitoring control applications, health care, security and environmental monitoring. Wireless sensor networks are an emerging technology consisting of small, low-power, and low-cost devices that integrate limited computation, sensing, and radio communication capabilities. Sensor network platform for health care has been designed, fabricated and tested. This system consists of an embedded micro-controller, Radio Frequency (RF) transceiver, power management, I/O expansion, and serial communication (RS-232). The hardware platform uses Atmel ATmega128L 8-bit ultra low power RISC processor with 128KB flash memory as the program memory and 4KB SRAM as the data memory. The radio transceiver (Chipcon CC1000) operates in the ISM band at 433MHz or 916MHz with a maximum data rate of 76.8kbps. Also, the indoor radio range is approximately 20-30m. When many sensors have to communicate with the controller, standard communication interfaces such as Serial Peripheral Interface (SPI) or Integrated Circuit ($I^{2}C$) allow sharing a single communication bus. With its low power, the smallest and low cost design, the wireless sensor network system and wireless sensing electronics to collect health-related information of human vitality and main physiological parameters (ECG, Temperature, Perspiration, Blood Pressure and some more vitality parameters, etc.)

• 대한인간공학회지
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• 제27권4호
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• pp.95-101
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• 2008

This research is to develop PPG filtering method for respiration measurement in U-Health Care system. Respiration rate was determined by filtering PPG and analyzing its spectrum. Optimal filter of PPG has been selected to get respiration by testing 120 sets of experiment data using 700 filtering cases. As a result, 2nd order Bessel-filter that used band-pass cutoff frequency at 0.175~0.4Hz with second order was good at developing respiration signal. Respiration signal in time domain could be continuously analyzed by converting frequency domain using spectrum analysis. 24 seconds has been found to be optimal time duration of collecting PPG data for determining respiration. Therefore, this study was successful of getting not only heart activity but also respiration by only PPG. Minimal invasive measurement obtaining multi-bio information by one sensor can be expected to apply to U-Health Care and human computer interaction.