• Title/Summary/Keyword: Health Properties

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Conditioned medium of E17 rat brain cells induced differentiation of primary colony of mice blastocyst into neuron-like cells

  • Budiariati, Vista;Rinendyaputri, Ratih;Noviantari, Ariyani;Haq, Noer Muhammad Dliyaul;Budiono, Dwi;Pristihadi, Diah Nugrahani;Juliandi, Berry;Fahrudin, Mokhamad;Boediono, Arief
    • Journal of Veterinary Science
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    • v.22 no.6
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    • pp.86.1-86.13
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    • 2021
  • Background: Conditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties. Objectives: This study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties. Methods: The conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium. Results: The conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium. Conclusions: Conditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.

Properties of Sunglass Lenses by Non-Destructive Test for the Sunglass Standards (비파괴식 검사를 통한 선글라스의 품질에 관한 연구)

  • Lim, Yong-Moo;Shim, Moon-Sik;Sim, Hyun-Seog;Kim, Sang-Moon
    • Journal of Korean Ophthalmic Optics Society
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    • v.6 no.1
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    • pp.125-131
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    • 2001
  • In this study, we compared the properties of 23 high and 55 low price sunglass lenses with the standards in the ordinary optical properties, materials, coloration, UV, IR and luminous transmittance, color acceptance for traffic signal, chromaticity and contrast sensitivity. The ordinary optical properties of the lenses met comparatively the requirements of the KS standard. The HIGH-type and LOW-type lenses were primarily made by glass and acrylate, respectively. In the coloration, HIGH-type was in group around neutral color but LOW-type was distributed widely on the line between 570 nm and 485 nm. There are fails in 7% of HIGH-type and 18% of LOW-type in the stimulus purity of the luminous transmittance. Wavelength of the UV/VIS cut-off was over 350 nm for HIGH-type but 6% of LOW-type was under 350 nm. In the erythemal UV, all HIGH-type met the needs of standards but 5 LOW-type failed with DIN standard In the near UV, KS standard worked in stringency, and HIGH-type showed more failure than LOW-type. The characteristics of the IR transmittance of HIGH-type was better than that of LOW-type. In the color acceptance of traffic signal, all HIGH-type met the needs of ANSI standards but 21.8% of LOW-type failed with the standard. In the contrast sensitivity tested with various coloured sunglasses, the value increased with increasing of Land decreasing of test distance. In view of the results so far, HIGH-type met with excellent properties as compared with LOW-type.

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Effect of Rheological Properties on the Sedimentation of Capsules in an Aqueous Polymer Solution (고분자 수용액의 레올러지 특성이 캡슐의 침강에 미치는 영향)

  • Kim, Dong-Joo;Kim, Jung-Ah;Kyong, Kee-Yeol;Yoon, Moung-Suk
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1 s.49
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    • pp.85-89
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    • 2005
  • An aim of this study is to study the correlation between rheological properties and sedimentation of capsules in aqueous polymer solution with low viscosity. Rheological properties of aqueous polymer solutions were controlled by carbomer (C), acylate/C10-30 alkyl acylate crosspolymer (AC), and ammonium acryloyldimethyltaurate/VP copolymer (AV). Small amount of polymer C solution had the highest viscosity and yield stress of polymer AV solution was higher than that of polymer C solution in the same viscosity when the concentration of polymer AV exceeded $0.35 wt\%$. Each aqueous polymer solution was tested and the results showed that as viscosity and yield stress increased, the sedimentation ratio of capsules decreased. The viscoelasticity data also showed the same tendency in a shear stress range of 0.1 to 2.0 Pa. These results demonstrated that the rheological properties of polymer solutions had a strong correlation with the sedimentation of capsules. When polymer I and AV were used, there was a synergistic effect and the correlation between rheological properties and sedimentation of capsules was very complicated. It was assumed that the characteristics of polymer structure and interaction between polymers caused this phenomena.

Rheological Properties of Calcium Phosphate Cement Mixed with 2 Kinds of Setting Solution (2종의 경화액과 혼합된 calcium phosphate cement의 유변학적 성질에 관한 연구)

  • Chang, Seok-Woo;Kwon, Ho-Beom;Yoo, Hyun-Mi;Park, Dong-Sung;Oh, Tae-Seok;Bae, Kwang-Shik
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.3
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    • pp.311-316
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    • 2008
  • Calcium phosphate cement (CPC) has been used as bone substitute successfully due to good biocompatibility and osteoconductivity. One of the important mechanical characteristics of CPC is flowablility, which can be evaluated by measuring rheological parameters. However, there have been few studies that measured rheological properties of CPC. The purpose of this study was to evaluate the rheological properties of CPC paste mixed with 2 kinds of setting solutions, 2% hydroxyprophyl methylcellulose (HPMC) and 35% polyacrylic acid (PAA). The CPC used was dicalcium phosphate dihydrate (DCPD). Rheological properties of CPC paste were measured using rheometer. The statistical analysis was carried out with Mann-whitney test with Bonferronis collection. CPC with both setting solutions showed shear thinning behavior. CPC with 2% HPMC showed signigicantly higher complex viscosity than CPC with 35% PAA(p<0.05).

Hair Strengthening Effect of Silane Coupling and Carbodiimide Chemistry (카르보디이미드 반응과 실란 커플링을 이용한 모발강화 효과)

  • Son, Seong Kil;Choi, Wonkyung;Lim, Byung Tack;Song, Sang-hun;Kang, Nae Kyu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.2
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    • pp.133-139
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    • 2018
  • Chemically damaged hair is vulnerable to external stimuli in daily life due to the weakened physical properties of the hair strand itself. The purpose of this work was to determine whether chemical conjugation between hair keratin proteins restores tensile strength and thus results inpreventing further deterioration under repeated combing. A model damaged hair tress was produced by a typical perm-process. Then, it was internally crosslinked by the bifunctional crosslinker (3-aminopropyl)triethoxysilane (APTES), via both silane coupling and carbodiimide chemistry. Physical properties, including tensile strength, Young's modulus, and plateau stress, were measured to verify the effect of internal crosslinking, and the existence of crosslinking was verified by Fourier transform infrared (FT-IR) spectroscopy. The degrees of hair breakage and split ends were evaluated by repeated combing-drying tests. Physical properties of chemically damaged hair were restored by internal crosslinking. Successful crosslinking of APTES via both silane coupling and carbodiimide chemistry was verified by FT-IR spectra. Prevention of breakage and split ends after repeated combing with heat was observed. Human hair can be weakened by chemical damage including perm-processing, so restoring such properties is a major issue in the hair care industry. This work shows that internal crosslinking of damaged hair via chemical conjugation would be a potent method to restore the healthy hair.

The Abnormal Rates of Blood Pressures and Blood Biochemical Properties with BMI in Health Checkup Examinees (건강검진 수검자들의 BMI에 따른 혈압, 혈액 및 혈액생화학적지표의 비정상치 분포)

  • Park, Kyu-Ri;Cho, Young-Chae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.12
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    • pp.4843-4853
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    • 2010
  • The purpose of this study was to investigate the association of blood pressures, hematological and biochemical properties of blood according to the degree of obesity in health checkup examinees. Study subjects were 3,731 adults of 20 years and over (2,312 males, 1,419 females), who underwent health package check-up at the Korea Health Management Association from Jan. 2007 to Dec. 2009. As a result, the rates according to the degree of obesity of study subjects were 4.6% in low weight group, 44.3% in normal weight group, 25.0% in over weight group and 26.1% in obesity group. The over weight group were significantly higher in 40's age group than other groups, and the obesity group were significantly higher in 50's age group than other groups. The abnormal rates of SBP, DBP, TG, TC, HDL-C, LDL-C, ALT, AST in male were significantly higher than that of female. With respect to degree of BMI, the abnormal rates of SBP, DBP, TG, TC, HDL-C, LDL-C, ALT, AST, GGT, ALP were significantly increased with higher level of BMI. The age-adjusted odds ratio in the abnormal level of SBP, DBP, TG, TC, HDL-C, LDL-C, AST, ALT and GGT were significantly increased in overweight group and obese group than that of normal weight group.

Reliability and Validity of the Greek Version of the Job Content Questionnaire in Greek Health Care Workers

  • Alexopoulos, Evangelos C.;Argyriou, Evangelia;Bourna, Virginia;Bakoyannis, Giorgos
    • Safety and Health at Work
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    • v.6 no.3
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    • pp.233-239
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    • 2015
  • Background: The Job Content Questionnaire (JCQ), which is based on the Demand-Control-Support model, is designed to measure the psychosocial characteristics of the respondent's work, and has been identified to predict health and psychological outcomes. The purpose of this study was to investigate the psychometric properties of this instrument and the subsequent adaptation of its scales to the population of Greek health workers. Methods: The Greek version of the JCQ was developed by using forward- and back-translation in accordance with the JCQ policy. The reliability and validity of the measure were investigated in a sample of health workers working in a hospital in Athens, Greece. The internal consistency of the scales was examined based on Cronbach ${\alpha}$ coefficients, and the validity was evaluated subjecting the items of the three main scales of the JCQ (decision latitude, psychological job demands, and social support) to exploratory and confirmatory factor analysis. Results: The reliability of the scales was found to be acceptable for all the scales, except for the skill discretion subscale. Confirmatory factor analysis confirmed a slightly modified version of the original construct including several items to more than one factor. Conclusion: Our findings suggest that the Greek JCQ is reliable and valid for investigating psychosocial job characteristics among Greek health workers.

Hypertension knowledge and Treatment compliance of Hypertensive Patients -Comparative analysis between groups treated at Primary Health Care Post in "H" Gun (country) and at other private Clinics·Hospitals. - (고혈압 환자의 고혈압지식 및 치료지시이행 -H군 관내 보건진료소와 병·의원 이용그룹 간 비교분석-)

  • Lee, Yang-Soon;Seo, Eun-ju
    • Journal of Korean Academy of Rural Health Nursing
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    • v.9 no.2
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    • pp.45-58
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    • 2014
  • Purpose: To determine the knowledge and treatment compliance with hypertension, directions for the aspects of implementation, and related factors to patients with hypertension using Primary Health Cares and Private Clinics Hospitals. Method: General characteristics and High blood pressure-related properties(17 questions), Hypertension knowledge measures(20 questions), and Treatment Compliance(22 questions) were used. Result: 1. The patients of Private Clinics Hospitals had statistically significantly higher drinking habits than Primary Health Care centers. The patients of Private Clinics Hospitals had statistically significantly higher rate of no family history of hypertension than Primary Health Care centers. 2. Primary Health Care centers had statistically significantly higher knowledge of hypertension than Private Clinics Hospitals. Primary Health Care centers had statistically significantly higher treatment compliance than Private Clinics Hospitals. Conclusion: Both Primary Health Care centers and Private Clinics Hospitals are high medication compliance but low lifestyle compliance with hypertension. We need to recognize the importance of lifestyle compliance, to apply proper programs and to provide therapists' aggressive intervention.

Domestic Rock Wool Toxicity Study on Respiratory System and Biopersistence Evaluation in Sprague-Dawely Rats (랫드에 주입된 국내산 암면의 호흡기 독성 및 생체내구성 평가)

  • Chung, Yong Hyun;Han, Jeong Hee;Kang, Min Gu;Lee, Sung Bae;Kim, Hyeon Yeong
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.19 no.2
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    • pp.127-138
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    • 2009
  • Rock wool, a kind of asbestos substitutes, was analyzed for its physicochemical properties. After fivers of rock wool were instilled into rat lungs, These pathological changes were evaluated. In addition, the fibers in the lungs were counted and characterized after the lungs were treated for electron microscopical analysis. The lungs of rats showed pathological lesions such as granulomatous changes, but these lesions disappeared at 28 days groups after instilled rats. The rock wool fibers in the lungs decreased more 50 % after 28 days instilled into rat lungs. And rock wool showed early change in fiber compositions after 3 days compare with chrysotile showed after 7 days instilled into rat lungs. This study showed that the durability of rock wool in the lungs is more milder than chrysotile.

A convergence study on oral health knowledge, attitude, behavior and oromaxillofacial trauma among physical education middle-high school students (체육 중·고등학생의 구강보건 지식·태도·행동 및 구강악안면 외상에 관한 융합 연구)

  • Choi, Min-Ji;Kim, Ji-Hwa;Oh, Na-Rae
    • Journal of the Korea Convergence Society
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    • v.7 no.5
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    • pp.35-42
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    • 2016
  • The purpose of this study is to investigate the properties about oral health of 250 physical education middle-high school students. From April 2013 to June, we researched their oral health knowledge, attitude, behavior and oromaxillofacial trauma using oral examination and structured survey and implemented independent t-test and frequency analysis. Female students were statistically higher in DMFT rate(Female 22.30 % and male 15.41% in average). Middle school students were higher in periodontal health status score compared to high school students(Middle school 1.19 and high school 0.68 in average). Female students scored higher in both oral health knowledge(Female 3.69 and male 2.81 in average) and behavior(female 38.26 and male 36.92 in average). Therefore, the implementation of oral health education and personalized oral health program that considers properties of physical education middle-high school students.