• 생약학회지
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• 제40권1호
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• pp.46-50
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• 2009

The antitumor activity of the roots extract of Pueraria thunbergiana was investigated on the basis of cytotoxicity upon the cultured human tumor cell lines, in vitro. The purification of methylene chloride (MC) soluble part and ethylacetate (EA) soluble part of extract by column chromatography furnished seven isoflavonoids, two triterpenoids, one but-2-enolide. The structures of them were established by chemical and spectroscopic means to be lupeol (1), $\beta$-sitosterol (2), biochanin A (3), (-)-tuberosin (4), calycosin (5), daidzein (6), puerarin (7), daidzin (8), (+)-puerol-B 2-O-$\beta$-glucopyranoside (9), formononetin-7-O-$\beta$-glucopyranoside (10). Each isolates ($1{\sim}10$) were evaluated for inhibitory activities on the proliferation of cultured human tumor cell lines such as A549, SK-OV-3, HCT-15 and SK-MEL-2, respectively.

• The Journal of Korean Physical Therapy
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• 제17권3호
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• pp.403-412
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• 2005

The purpose of this investigation is examining how treatment for foot reflex massage affect the changes in body temperature and blood composition. The cases of experiment are 10 male(n=2) and female(n=8) who don't have special clinical problems. This is the process of experiment. First, we photograph the anterior part and posterior of the upper, lower half of the body with an infrared imaging system. Second, among same parts, we compare the temperature of the whole body before treatment for foot reflex massage with the temperature after it. Then we draw blood from the body and observe a change: in numerical value of blood before and after treatment. Before and after treatment, we come to measure temperature of the whole body, the numerical value of WBC, RBC, platelet, HCT, Hb through analysis of blood, and the result of AST(GOT), ALT(GPT), ALP, GGT through analysis of serum enzyme activity of blood. There were no significant differences in blood composition. In terms of SPSS statistic program, when we explain each numerical value before and after foot massage, the abdominal, the buttocks, the femoral region universally take successful results about a change in body temperature(p<0.05).

• 동의생리병리학회지
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• 제20권3호
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• pp.563-571
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• 2006

The purpose of this study was to identify the effects of oriental herbal drink to improve aerobic capacity in elite endurance runners. 14 male elite college runners were participated and divided into two group; i ) oriental herbal drink group (OG), ii ) placebo group (PG). All subjects were completed treadmill exercise protocol using GXT at before (B) and after (A) experimental treatment of one week. The V02max, anaerobic threshold (AT) were measured by gas analysis and heart rate (HR) were measure by polar system at pre, max, post, post 30 min and post 60 min. Blood samples were collected to analyze blood components. The V02max were significantly increased in OGA compared to OGB whereas the V02max and AT in OGA were significantly higher than PGA. The blood lactate concentration were shown higher decrease rate in OGA compared to Doth OGB and PGA during recovery whereas LDH and Na were significantly increased in OGA compared to both OGB and PGA. The blood concentrations of CI and K were significantly increased in OGA compared to PGA. There were no significant differences were founded in WBC, RBC, Hct, Hb and other components associated with energy sources(glucose, TG, TC, HDL, LDL, creatinine, CPK). These results suggested that this oriental herbal drink can be administrated to improve aerobic capacity in long distance runners.

• 대한수의학회지
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• 제35권1호
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• pp.137-142
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• 1995

Anaplasmosis is a tick-borne disease of large and small ruminants, causing losses through mortality, abortion, weight loss and reduced milk production. In one dairy farm, for example, 250 of a total of 800 imported goats were diagnosed with a mysterious type of anemia during the summer and autumn of 1992. The etiologic agent was identified as Anaplasma spp by acridine orange and ultrastructure by electron microscopy. In order to monitor variations in blood biochemical and hematological parameters associated with the disease, blood samples were collected by jugular venipuncture from 50 goats at 3 month intervals between the period of February and October, 1993. The levels of RBCs, HB and HCT decreased from $18.48{\pm}1.96$ to $13.47{\pm}2.48X10^6/mm^3$, $12.25{\pm}1.41$ to $9.54{\pm}1.77g/dl$, and $43.09{\pm}4.75$ to $30.93{\pm}5.78%$, respectively. The values of MCH(Mean corpuscular hemoglobin), MCHC(Mean corpuscular hemoglobin concentration) and PLT(Platelet) were elevated from $6.58{\pm}0.30$ to $7.05{\pm}0.47pg$, $28.40{\pm}1.20$ to $30.82{\pm}1.85g/dl$ and $1688.34{\pm}750$ to $2046.82{\pm}783X10^3/mm^3$, respectively. Percent parasitized erythrocytes(PPE) increased from $0.61{\pm}0.5$ to $2.22{\pm}1.9%$, clinical biochemical parameters aspartate aminotransferase and alanine aminotransferase were $66.64{\pm}23.1K.U$ and $14.90{\pm}6.59K.U$, respectively and persisted at high levels throughout the observation period. The level of albumin(2.46)0.52 g/dl) was decreased corresponding to an elevated globulin and a reduced albumin/globulin ratio in October as compared with the values in February. It is concluded that caprine anaplasmosis may be an important cause of anemia and hepatic malfunction in goats.

• 대한수의학회지
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• 제36권1호
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• pp.221-233
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• 1996

This experiment was carried out to study the toxic effect of solublized methylcellulose (MC). Sprague-Dawley rats were dosed with 1%(w/v) MC in 0.9% saline by gavage at a dose of 10ml/kg b.w/day or by intravenous injection at a dose of 5ml/kg b.w/day for 28 days. Clinical signs were observed once a day. Body weights, water and food consumptions were measured and urinalysis was performed several times during the experiment. Rats were sacrificed on days 3, 7, 15 and 28 for hematology, blood chemistry, organ weights and histopathology. The relative weight of the spleen and foamy cells of the spleen were increased in the gavage group. Body weight gain, food consumptions, the values of RBC, Hb, MCH, Hct, serum proteins, glucose, bilirubin, AST, and ALP were decreased in I.V. treatment group. On the other hand, water consumptions, the values of serum cholesterol, creatinine, and BUN were increased. Microscopic findings were granulomas, distended sinusoids, and hypertrophy of Kupffer cells with vacuoles in the liver. Spleen exhibited granuloma, increased extramedullary hematopoiesis, and congestion. Kidney exhibited foamy cells in the glomeruli, distension of the tubules. The findings appeared more severe when the treatment was extended. In conclusion, MC solution is not a safe vehicle for intravenous administration because of the toxic effects on the liver, kidney and spleen. In addition, a long-term and large dosage of oral administration of MC appears to be unsafe also and needs to be investigated further.

• 대한지역사회영양학회지
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• 제3권5호
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• pp.715-721
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• 1998

This study was performed to investigate how milk supplementation can benefit the elderly by increasing bone density but possibly harming the iron status concomitantly. Forty one elderly subjects over 65 years of age(male : 9, female : 32) participated. All subjects were apparently healthy, home staying and attending meal service for lunch at the welfare center. They were from low income area of Puchon city. One cup of milk per day was served for 10 months. The mean intake of calcium was significantly increased for females after milk supplementation. Males showed significantly increased means of triceps skinfold thickness, suprailiac skinfold thickness and waist circumference. Females showed significantly increased measurements fo three kinds of skinfold thickness, waist circumference, and hip circumference. There were no significant change in the mean bone density of lumbar spine$(L_2~L_4)$, femoral neck, ward's triangle and torchanter, but the proportion of osteopenia estimated by the T score of lumbar spine bone density was lowered from 50.0% to 34.6% for females. The mean Hb level was significantly for males. The proportion of anemia estimated by Hb(<12g/dl), Hct(<36%) and serum ferritin(<15mg/ml) were increased from 17.2% to 51.7%, from 20.7% to 44.8% and from 10.3% to 17.2%, respectively for females. It looks like milk supplementation can effect the intakes of several nutrients considered to be commonly deficient in the Korean diet fo elderly people, increase some anthropometric measurements, and decrease the proportion of osteopenia. However it can have adverse effects on iron status of females.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.103-110
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• 2020

Objective: The objective of our study was to investigate anti-cancer effects of Danggui Liuhuang Decoction extract bioconverted by protease liquid coenzyme of Aspergillus kawachii (DLD-BE), compared to a non-bioconverted DLD extract (DLD-E) and determine the underlying mechanisms. Methods: DLD-E and DLD-BE were evaluated for their ability to modulate these signaling pathways and suppress the proliferation of human colorectal cancer (CRC) cells, HCT-116, LoVo, and HT-29. The anti-cancer effects of DLD-E and DLD-BE were measured by using proliferation and migration assays, cell cycle analysis, Western blots, and real-time PCR. Results: In this study, treatment with DLD-E and DLD-BE at concentrations of 25-100 ㎍/mL inhibited proliferation and migration in human CRC cells. DLD-BE induced apoptotic cell death and decreased COX-2 expression in HT-29 cells. The mechanisms of action included modulation of the AKT and extracellular-signal-regulated kinase signaling cascades along with inhibition of COX-2 expression. The results demonstrate novel anti-cancer mechanisms of DLD-BE against the growth of human CRC cells. Thus, we propose that DLD-BE can be developed as a more potent supplement to inhibit colorectal tumor growth and intestinal inflammation than DLD-E.

• 한국작물학회지
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• 제60권4호
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• pp.498-503
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• 2015

This study was conducted to evaluate the cytotoxicity and ${\alpha}-Amylase$, ${\alpha}-Glucosidase$, pancreatic lipase inhibition in vitro by different solvent fractions from the roots of Codonopsis lanceolata. The values of $IC_{50}$ against Calu-6 cell showed a high effect in n-hexane fraction ($10.13{\mu}g/mL$) whereas DW fraction exhibited the weakest inhibition on cell viability, having an $IC_{50}$ value of over $1,000{\mu}g/mL$. The values of $IC_{50}$ against HCT-116 cell showed the highest activity in n-BuOH fraction ($102.01{\mu}g/mL$), followed by n-hexane fraction ($145.85{\mu}g/mL$), methylene chloride fraction ($332.02{\mu}g/mL$), ethyl acetate fraction ($462.93{\mu}g/mL$) and DW fracion ($>1,000{\mu}g/mL$). ${\alpha}-Amylase$ inhibitory activity in methylene chloride fraction and ethyl acetate fraction was found to have a higher inhibitory effect with 24.5% and 25.6% than the other fractions. The highest ${\alpha}-Glucosidase$ inhibitory activity was observed from the ethyl acetate fraction extract, while the extract of DW fraction showed the lowest level of inhibitory activity at given experiment concentration. The pancreatic lipase inhibitory activity of C. lanceolata was found to have a higher the effect in ethyl acetate fraction. Inhibition of lipase activity of the ethyl acetate fraction and n-hexane fraction showed a relatively high, while the extract of DW fraction showed the lowest level at given experiment concentration. These results suggested that the roots of C. lanceolata may assist in the potential biological activity on carbohydrate, lipid Inhibitory activity and anticancer activity.

• 대한임상검사과학회지
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• 제36권2호
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• pp.137-143
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• 2004

Erythrocyte sedimentation rate(ESR) measurements were performed in a total of 191 blood samples from patients below a hematocrit of 35% by both the Test-1(SIRE Analytical Systems, Udine, Italy) and the Westergren method, endorsed as the reference method for ESR by the International Council for Standardization in Hematology(ICSH). The corrected Westergren ESR values were also obtained applying the formula of Fabry (corrected ESR = ESR measured x 15/55-Hct). Linear regression analysis showed a close correlation (r=0.85) between the two methods both before and after ESR correction in total samples. There was no significant correlation difference between two methods in both two groups with hemoglobin $${\geq_-}9g/dl$$ and <9g/dl before and after ESR correction (r=0.873, r=0.827 respectively before correction and r=0.867, r=0.830 respectively after correction). Also, for two groups with hematocrit $${\geq_-}24%$$ and <24%, no significant difference was observed (r=0.859, r=0.792 respectively before correction and r=0.782, r=0.842 respectively after correction). However, for samples with <60mm of Westergren ESR before correction, a better correlation coefficient was obtained than samples with $${\geq_-}60mm$$(r=0.701, r=0.541 respectively). In corrected Westergren ESR, also samples that were obtained with <40mm showed better correlation than samples with $${\geq_-}40mm$$ (r=0.690, r=0.347 respectively). In conclusion, we found a good correlation between Test-1 and Westergren measurements in patients with anemia but an expert group discussion is required to solve discrepancy between two methods in blood samples with very high ESR.

• 대한유전성대사질환학회지
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• 제2권1호
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• pp.89-94
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• 2002

목 적 : 한국에서 처음 개발된 페닐케톤뇨증 소아를 위한 특수분유를 1년간 섭취시켜 발달양상 및 치료결과를 관찰하여 효율성과 안정성을 확인하고자 하였다. 방 법 : 순천향대학병원에서 페닐케톤뇨증으로 진찰된 소아 14명을 대상으로 1년간 수유하면서 식이 일기 평가, 혈장 아미노산 분석, 일반혈액검사, ferritin, 간기능검사, 소변검사를 시행하였다. 결 과 : 14명 모두 잘 먹었으며 신장, 체중, 두위는 모두 정상발육을 보였다. 혈색소는 모두 정상범위였다. 14례중 4례에서 ferritin 치가 12 ng/mL 이하여서 철분체제를 투여하였다. 알부민 치, S-GOT, S-GPT 소변검사는 모두 정상이었다. 페닐알라닌 치는 0.7-15.6 mg/dL로 대부분 정상 범위로 잘 유지되었다. 발진, 설사, 변비 등의 부작용은 관찰되지 않았다. 결 론 : 국내에서 처음 개발된 PKU-1과 PKU-2 Formula의 발육양상과 치료 결과가 좋은 것으로 확인되어 환자에게 안심하고 사용할 수 있는 우수한 제품으로 사료된다.