• Applied Biological Chemistry
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• 제44권3호
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• pp.143-147
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• 2001

Hemoglobin(Hb) 가수분해도(degree of hydrolysis, DH)를 간편하고 신속하게 측정하고자 보편적으로 이용되는 TNBS법(trinitrobenzene sulfonic acid method)에 의한 가수분해도와 osmometer를 이용한 방법의 상관관계를 효소반응 조건에 따라 조사하였다. 다양한 ph 범위$(pH\;7.5{\sim}10.0)$에서 DH에 대한 두 방법의 상관계수($R^2$값)는 $0.974{\sim}0.991$로 매우 높은 값을 보였으며, 각각 측정한 DH 사이의 비율($DH_{OSM}/DH_{TNBS}$은 $1.438{\sim}1.656$의 범위에 있었다. Hb을 가수분해하여 heme-iron을 얻기 위한 특정 반응조건인 pH 7.5, $50^{\circ}C$에서 Esperase와 Fla-vourzyme을 각각 사용한 경우와 Esperase와 Flavourzyme을 혼용한 경우에서도 두 방법간의 DH 비율은 효소의 종류 및 처리방법에 무관하게 1.658을 나타내어 Esperase만을 사용한 경우와 매우 유사하였고 두 방법간의 상관계수는 0.982이었다. 따라서, 두 방법간의 상관관계식으로부터 osmometer를 이용한 Hb의 가수분해도를 간편하고 신속하게 측정할 수 있었다.

• 기본간호학회지
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• 제14권1호
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• pp.44-52
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• 2007

Purpose: This study was done to investigate the effect of Tai Chi Qigong exercise program on insulin resistance and blood glucose in patients with type 2 diabetes. Method: This was a quasi-experimental study used a non-equivalent control group pretest-posttest design. Participants included 39 patients with type 2 diabetes mellitus patients (ages between 40-70)(experimental group, 19, control group, 20). The Tai Chi Qigong exercise program consisted of 5 minute warming up, 30-minute main exercise, and 5 minute cooling down, and was conducted twice a week for 10 weeks. The outcome variables were insulin resistance (proinsulin, C-peptide, insulin, insulin sensitivity index) and blood glucose (fasting, post prandial 2hr, HbA1c). Data was analyzed using ANCOVA, with pre-test values as covariates, to examine difference between pre and post measures between the two groups. Results: The Tai Chi Qigong was effective in lowering postprandial 2hr blood glucose. Conclusion: Twice a week 10 week-Tai Chi Qigong exercise seems to be too short to improve insulin excretion or resistance, and fasting blood sugar and HbA1C in patients with type 2 DM. A Tai Chi Qigong program at least 3 times per week or longer is recommended.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.842-844
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• 2000

Changes in respiration rate (RR), rectal temperature (RT), respiratory moisture loss (RML), packed cell volume (PCV) and haemoglobin (Hb) were monitored in 2 adults female goats each of the Saanen (S) and Toggenburg (T) breeds during 60 min of exercise (walking at 3 km/h) on a moving-belt treadmill on each of 6 alternate days. A significant $time{\times}breed$ interaction was observed for RR; mean values in Sand T after 60 min of exercise were 130 and 223 /min ($p{\leq}0.01$). The observed time x breed interaction for RT indicated that S was less stressed by exercise than T; mean values after 60 min exercise were 40.4 and $40.8^{\circ}C$ respectively ($p{\leq}0.01$). For RML, the $day{\times}breed$ interaction ($p{\leq}0.001$) indicated that while S had higher values on day 1, thereafter the values for T were higher. The $time{\times}breed$ interaction for RML/breath indicated that values for T declined more rapidly (from 9.4 to 3.1 mgjbreath) than those for S (from 8.3 to 4.1 mgjbreath; ($p{\leq}0.01$). PCV declined during exercise ($p{\leq}0.05$) by 5.5 percentage points. The exercise imposed was stressful in that it led to increases in RR, RT and RML. S was most tolerant of exercise in that it recorded lower values of RT. The fact that the RML/breath was higher during exercies in S apparently allowed it to compensate for a lower RR. Despite higher RR and RML, T also had a higher RT, suggesting either higher muscular heat production during exercise in that breed, or higher sweating losses in S.

• 한국주조공학회지
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• 제16권2호
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• pp.109-115
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• 1996

Effect of various austempered structures on fracture characteristics of C/V graphite cast iron has studied. The tensile strength and hardness reached the maximum value of 971.4MPa and HB302 at the austempering temperature of $250^{\circ}C$, respectively. As the austempering temperature increased, the amount of retained austenite increased from 18% to 22, 29%, while $K_{IC}$ values ranged from the value of $65MPa{\cdot}m^{12} to 70MPa{\cdot}m^{1/2}, 66MPa{\cdot}m^{1/2}. This fact that $K_{IC}$ value was not sensitive to the increase of the amount of the retained austenite was that $K_{IC}$ was dependent on the matrix structure in lower bainitic matrix, while dependent on the notch effect from C/V graphite shape in upper bainitic matrix. Fractured surfaces showed a ductile fracture pattern at $300^{\circ}C$. Very large coalescence by C/V graphite and relatively small voids by spheroidal graphite were observed.

• Journal of Nutrition and Health
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• 제22권6호
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• pp.457-465
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• 1989

Guar gum, a storage polysaccharide galactomannan was administered to 11 patients with type-II diabetes mellitus for 7 days and 3 weeks. They took 5 grams of guar gum 30 mins before each of three meals daily. The dinner 2-h postprandial values of their blood glucose were significantly lowered (P<0.05) after their guar treatment for 7 days compared with before taking guar gum. The 2-h postprandial values of blood glucose were significantly lowered(P<0.05) after 3 weeks of gual treatment compared with before taking guar gum. In an oral glucose tolerance test, their blood glucose values were significantly lowered at 120 mins(P<0.02) and 180 mins(P<0.05) after guar treatment. Total-lipid(P<0.01) and triglycerides(P<0.02) of their blood were significantly decreased and HDL-cholesterol(P<0.02) was significantly increased after guar treatment. HbA1C was significantly reduced (P<0.05) from 11.3% to 10.1% The body weight, total-cholesterol and insulin activity of the patients after guar treatment were not significantly changed and the satiety ratings of the patients with guar treatment was not significantly changed and the satiety ratings of the patients with guar treatment was not significantly changed, however, the subjects that answered from 'Want to eat but can wait' to 'No desire to eat' were 81.1%. It is concluded that guar gum improves their carbohydrate and lipid metabolism in Korean type-II diabetic subjects with high fiber diets.

• 한방재활의학과학회지
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• 제18권2호
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• pp.97-109
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• 2008

Objectives : Effects of Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) on intellectual development and learning ability were investigated growth and intellectual impairment rat with insufficient nutrition diet. Methods : We divided male Sprague-Dawley rats into 4 groups(A, B, C, D). They were normal group(A), growth deficiency rat with insufficient nutrition diet group(B), growth deficiency rat with 0.1% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group(C) and growth deficiency rat with 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group(D). They were administered for 5 weeks. We measured body weight, serum growth hormone, insulin-like growth factor and thyroid stimulating hormone, RBC, concentration of Hb and PCV ratio, total WBC and its composition, the values of plasma glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) activities and morris water maze test in escape distance, escape time and escape speed. Results : 1. Body weight showed a tendency to increase in the Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups and 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group showed significantly different than control groups. 2. Serum growth hormone, insulin-like growth factor and thyroid stimulating hormone showed a tendency to increase in Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups, however these values showed no significantly different. 3. About the counts of RBC, 0.1% and 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed significantly different than control groups. Concentration of Hb was higher than control group in Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups. And 0.2% Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) group showed significantly different than control groups in PCV ratio. 4. The counts of total WBC and its composition showed no significantly different in all treatment groups. 5. The values of plasma glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase(GPT) activities showed no significantly different in all treatment groups. 6. In the morris water maze test, in escape distance and escape speed, Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed no significantly different than control group. But Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) groups showed the increasing tendency. Conclusions : So Kyungohkgo($Qi{\acute{o}}ng\;y{\grave{u}}\;g{\grave{a}}o$) have an effect of promoting growth of rats and might be effect to treat various kinds of growth delay in children.

• Journal of Animal Science and Technology
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• 제59권1호
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• pp.2.1-2.6
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• 2017

Background: This study aimed to analyze the on heart rate, blood lactate concentration, packed cell volume (PCV) and hemoglobin (Hb) response after conducting exercise in endurance horses. Methods: A total of 20 healthy 3-9-years-old Jeju crossbreed mares ($5.95{\pm}2.24$ year) of age and $312.65{\pm}13.59kg$ of weight) currently participating the endurance competition were used. The field tests selected for the experiment was gallop (approximately 8.3 m/s) along the selected 2.5 km course (a natural forest trail, not artificial road; a closed loop course). The horses were divided into three groups according to their age; 3-4 years of age (G1, $3.29{\pm}0.49$ year), 6-7 years of age (G2, $6.42{\pm}0.53$), and 8-9 years of age (G3, $8.50{\pm}0.55$). The measurements times for the heart rate, blood lactate concentration, PCV, and Hb analysis were conducted before exercise (T0), shortly after exercise (T1), 15 min after exercise (T2), and 30 min after exercise (T3), respectively. Data was analyzed using an analysis of covariance (ANCOVA) for repeated measures with times and groups. Results: The results of the comparison depending on the passage of rest time after exercise suggest that the heart rate and blood lactate concentration of three groups at T2 significantly decreased compared to T1 (p < 0.001). PCV of the G2 and G3 groups were significantly decreased at T2 compared to T1 (p < 0.01). Hb values at G2 (p < 0.01) and G3 (p < 0.001) groups were significantly decreased at T2 as compared to T1. However, heart rate, blood lactate concentration, PCV and Hb level at T1 showed no difference in the comparison of horses from different age groups with the exception of G3 group in terms of heart rate. Conclusion: The physiologic and hematological responses of horses during recovery time after 2,500 m exercise with gallop were no significant difference among the groups. These data are useful as a response evaluation method for training of endurance horses.

• Journal of Preventive Medicine and Public Health
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• 제19권2호
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• pp.167-176
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• 1986

For the purpose of determinating the normal values of some parameters relevant to lead exposure, a study was carried out from April 1 to June 30, 1986 on 258 healthy Korean adults who have had no apparant lead exposure. The lead indices subjected to this study were as follows; blood lead (PbB), hemoglobin (Hb), zinc protoporphyrin in blood (ZPP), delta-aminolevulinic acid dehydratase (ALAD) activity in blood, coproporphyrin in urine (CPU), delta-aminolevulinic acid in urine (ALAU). 1) The mean value of PbB was $17.17{\pm}7.87{\mu}g/100ml$, and there was no statistically significant difference by age & sex. The distribution of PbB fitted to the log-normal distribution ($x^2=7.38$, p>0.1). 2) The mean value of Hb in male ($15.17{\pm}1.56g/100ml$) was higher than in female ($13.22{\pm}1.51g/100ml$)(p<0.01). The distribution of Hb fitted to the normal distribution ($x^2=9.40$, p>0.1). 3) The mean value of ZPP was $32.61{\pm}8.78{\mu}g/100ml$, and there was no statistically significant difference by age & sex. The distribution of ZPP fitted to the normal distribution ($x^2=13.93$, p>0.05). The correlation of ZPP & ALAD (r=-0.229), CPU (r=0.183) was statistically significant respectively. 4) The mean value of ALAD was $30.20{\pm}10.96{\mu}mol$ ALA/min/L of R.B.C., and there was no statistically significant difference by age & sex. The distribution of ALAD activity did not fit to the normal distribution. The correlation between ALAD & PbB (r=-0.219) was statistically significant 5) The mean value of CPU was $36.10{\pm}24.54{\mu}g/L$, and there was no statistically significant difference by age & sex. The distribution of CPU did not fit to the normal distribution. The correlation between CPU & PbB (r=0.185), ZPP (r=0.183) was statistically signinificant respectively. 6) The mean value of ALAU was $1.94{\pm}0.96mg/L$, and there was no statistically significant difference by age & sex. The distribution of ALAU fitted to the normal distribution ($x^2=9.76$, p>0.1).

• Journal of Preventive Medicine and Public Health
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• 제33권3호
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• pp.349-363
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• 2000

Objectives : Generally, it seemed that the therapeutic result in diabetic patients was changed by compliance. This study was conducted on the basis of assumption that the therapeutic result id diabetic patients could control according to compliance. This study was conducted to analyze the related factors in association with compliance to drug, diet and exercise therapy. Methods : 224 diabetic patients in Kyungpook National University Hospital were selected through the interviews and HbA1c values from 1 Jan. to 28 Feb.1997. The drug compliance was tested by regularity of drug administration, the diet compliance was tested by restriction of food, exactly allocation, balance of nutrient, measuring food and the exercise compliance was tested by regularity of exercise per day. We assessed compliance by percentage, $x^2-test$ and generalized logit regression model(method:enter). Results : The significant variable was the satisfaction to medical personnels in drug, the knowledge to disease in diet, the participation of the diabetic education in exercise therapy and the satisfaction to medical personnels in HbA1c. Using the generalized logit model(method : enter) in compliance change, the significant variables were the satisfaction to medical personnels and the complication in drug; the significant variables were the age at the first diagnosis, the family history, the concern of health, the knowledge of disease, the self-exertion for therapy and the complication in diet: the only significant variable was the gender in exercise therapy. Conclusions : The degree of glycemic control in diabetic patients was influenced by compliance. In order to improve patient's compliance, we must foster the knowledge on the diseases, lead participation for diabetic education. Because the satisfaction to medical personnels was the important variables, we must build up good relationship between doctors and patients.

• Journal of Microbiology and Biotechnology
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• 제4권2호
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• pp.126-133
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• 1994

A Pseudomonas putida strain able to accumulate high amount of polyesters of medium-chain-length 3-hydroxyalkanoic acids ($PHA_{MCL)$) was isolated from soil in a landfill site using an enrichment technique. Culture condition of the isolated strain for polyester production in a one-step culture was optimized in a mineral-salts medium against pH and concentrations of ammonium sulfate, carbon source(e.g., octanoate), and phosphate. The optimal values for maximal cell growth and PHA accumulation were: pH; 7$\sim$8, $(NH_4)_2SO_4$; 8 mM, octanoate; 40 mM. The optimum temperature was in the range of $20\sim30^{\circ}C$, which was rather broader than in other bacteria. Cell growth was strongly inhibited by the phosphate limitation to less than 1 mM. An increase of phosphate concentration above 1 mM showed little effect on cell growth and polyester accumulation. When the strain was grown on octanoate under this optimized condition it produced 3.4 g dry biomass per liter and yielded 1.7 g PHA per liter amounting to 53 wt% of dry cells. The monomer units composing the polyester synthesized from octanoate were 3-hydroxyoctanoate (3HO), 3-hydroxycaproate (3HC), and 3-hydroxybutyrate (3HB) (85:13:2, mole ratio). Other low linear $C_3\simC_{10}$ monocarboxylic acids were also tested for polyester production.