• Asian-Australasian Journal of Animal Sciences
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• 제17권7호
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• pp.960-966
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• 2004

The purpose of this study was to evaluate the effects of protein supplementation of green tea waste (GTW) on the performance of lactating cows. Another aim was to increase resource utilization and to eliminate any environmental negative impact from the tea waste. GTW from a beverage company was ensiled at a low pH (<4.0) and high acetic acid and lactic acid concentration, and it contained high crude protein (CP, 34.8%), total extractable tannins (TET, 9.2%) and condensed tannin (CT, 1.7%). Two experiments were conducted to investigate the palatability and performance in lactating cows fed GTW. In the palatability trial, three lactating cows were allocated to three dietary treatments in a 3$\times$3 Latin square design. The animals were offered a total mixed ration (TMR) including GTW at rates of 0, 2.5 and 5.0% on a dry matter (DM) basis. Total DM intake was not different among the treatments. In the performance trial, four lactating cows were used in a 2$\times$2 Latin square design with a 3 week sampling period. GTW was incorporated into TMR at a rate of 5.0% on a DM and 10.0% on a CP basis. Thus GTW replaced alfalfa hay and soybean meal at a level of 25.0% on a DM. DM and CP intake were not affected by the inclusion of GTW, whereas TET and CT intake were significantly increased (p<0.001). Milk production, milk composition and the efficiency of milk production were not altered by the GTW inclusion. Although ruminal pH and VFA, and blood urea nitrogen were not changed, ruminal $NH_{3}-N$ and plasma total cholesterol were relatively low in the GTW group, but not significantly different. The excretion of urinary purine derivatives and estimated MN supply were also not significantly affected by GTW treatment. It is therefore concluded that GTW can be used as a protein source without any detrimental effects on the performance of lactating cows.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1881-1889
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• 2018

Objective: In dryland areas of China, alfalfa hay (AH) is a possible substitute for concentrate feed for beef cattle. To evaluate the potential benefits of this substitution, we studied the effect of the ratio of AH intake to total dry matter (DM) intake on average daily body-weight gain (ADG), dietary energy utilization status, and economic benefit in Gansu province. Methods: In each of two feeding trials in 2016 (trial 1 [T1], July 3 to 17; trial 2 [T2], August 15 to September 23), crossbred male Simmental calves were allocated to low AH (LA), medium AH (MA), and high AH (HA) feeding groups (n = 4 per group). The target ADG was set as 1 kg for both trials. In a one-way-layout design based on conventional feeding practices in the province, calves received diets containing the different AH amounts, with a constant ratio of corn stover:total DM and decreasing rations of concentrate feed proportional to the increase in AH. Calves in T1 received AH at 15% (T1-LA), 23% (T1-MA), or 31% (T1-HA) of their dietary DM allowances; those in T2 received 9% (T2-LA), 24% (T2-MA), or 34% (T2-HA) AH. Results: Among the T1 groups, both ADG and economic benefit were highest in T1-LA; whereas in T2, they were higher in the T2-LA and T2-MA groups than in T2-HA. Energy digestibility did not significantly differ among the groups in either trial. The dietary AH inclusion ratios of 14% in the warm season and 8% to 21% in the cool season appeared to yield optimal ADG, metabolizable energy intake, and economic benefit. Conclusion: Low-level inclusion of AH, ranging from 8% to 21%, is a practical approach for beef cattle feeding. This modified feeding regimen likely will promote increased growth performance during the fattening stage of beef steers in dryland areas of Gansu province, China.

• 한국초지조사료학회지
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• 제44권2호
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• pp.92-98
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• 2024

본 연구는 수입산 알팔파와 열풍 건조 국내산 알팔파를 이용한 TMR의 혼합 급여가 착유우의 우유 생산량, 유질 및 반추 시간 비교 분석 등을 통해 열풍 건조 국내산 알팔파의 사료가치 평가를 수행하였다. 두 종류의 알팔파 내 TDN, NE_L 및 체중은 차이를 보이지 않았고, 사료 섭취량은 열풍 건조 국내산 알팔파의 TMR 혼합 급여구에서 높았으며, 반추 활동 시간은 차이를 보이지 않았다. 우유생산량, 유지방과 유단백질 함량, FPCM, FE 및 SCC는 차이를 보이지 않았으며, 유지방 생산량과 lactose 함량은 열풍 건조 국내산 알팔파 TMR 혼합 급여구에서 높았다. 따라서 본 연구에서 이용된 열풍 건조 국내산 알팔파와 TMR의 혼합 급여는 착유우의 사료 섭취량, 반추 활동 및 생산성에 차이를 보이지 않았으므로, 열풍 건조 국내산 알팔파를 수입산 알팔파로 대체하여 착유우에게 이용될 수 있을 것으로 사료된다.

• 한국초지조사료학회지
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• 제19권2호
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• pp.127-132
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• 1999

우리나라에서는 봄철에 콩과목초를 수확한 후 포장 건조시 목초의 수량 및 품질손실에 관한 연구는 거의 없는 실정이다. 따라서 본 시험은 콩과목초인 알팔파(Medicago sativa L.), 크림손 클로버 (Trifolium incarnatum L.) 및 레드 클로버(Trifolium pratense L.)를 봄철 포장에 건조하였을 때 콩과목초의 수량 및 품질 손실에 관한 정보를 얻기 위하여 수행하였으며 결과는 다음과 같다. 알팔파의 수확시 건물 함량은 15%로 가장 높았으며, 크림손 및 레드 클로버의 건물 함량은 각각 12.4 및 11.5%였다. 이러한 초종간의 건물 함량의 차이는 포장 건조기간 중에 계속 유지되었다. 건물 함량이 80% 이상이 되는데 알팔파는 7일이 소요되었으나, 크림손 및 레드 클로버는 8일이 소요되었다. 크림손 클로버 및 알팔파의 건물당 잎과 줄기의 비는 레드클로버보다 높았으나, 조단백질 함량을 가지고 비교할 때 잎과 줄기 비는 레드 클로버가 크림손 클로버 및 알팔파보다 높았다. 건조기간중 탈엽에 의한 건물 손실률은 알팔파가 크림손 클로버 및 레드 클로버보다 높았으며, 조단백질의 손실률도 초종간의 차이가 같은 경향을 보였다. 생초와 건초의 사료가치 비교에서 생초는 조단백질(CP), 가소화영양소총량(TDN) 및 상대사료가치(RFV)에서 높았고, 건초는 ADF 및 NDF 함량이 낮았다. 이상의 연구결과를 볼 때 봄철(5월 중순)에 포장에서 건조된 건초는 건조기간 중 탈엽에 의한 조단백질의 손실이 높기 때문에 ADF 및 NDF 함량보다는 조단백질 함량에 더 영향을 미치는 것을 알 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권6호
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• pp.859-863
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• 2018

Objective: Since very little information exists about the topic; in this experiment we compare, in a long-term finishing program, the growth-performance responses and carcass characteristics of Holstein steers where non-structural carbohydrate concentration of the diet is reduced from 64% to 51% (dry matter basis). Methods: Sixty Holstein steer calves ($129{\pm}2.2kg$) were blocked by initial weight into five groups and randomly assigned within weight groupings to 10 pens. Calves were fed with a steam-flaked corn-based finishing diets containing 51% higher fiber (HF) or 64% lower fiber (LF) nonstructural carbohydrates. Non-structural carbohydrates concentrations were manipulated substituting dried distiller grain with solubles and alfalfa hay for flaked corn. Cattle were weighed every 112 days and at the end of the experiment (day 308) when the cattle were harvested and carcass characteristics were evaluated. Results: Steers fed the HF diet showed improvement (8.8%) in average daily gain (ADG) during the initial 112-d period. This effect was followed by a numerical trend for greater ADG throughout the remainder of the study so that overall ADG tended to be greater (4.9%, p = 0.06) for the HF than for LF. There were no treatment effects on dry matter intake. Gain efficiency and estimated dietary net energy (NE) were greater 8.3% and 5.2%, respectively for HF during the initial 112-d period. Overall (308-d) gain efficiency and estimated dietary NE were similar for both dietary treatments. However, due to differences in tabular dietary NE, the ratio of observed:expected dietary NE tended to be greater (4.1%, p = 0.06) for the HF vs LF diet. There were no treatment effects on carcass characteristics except for a tendency toward a slightly greater (0.5%, p = 0.09) estimated carcass yield. Conclusion: Reducing the non-structural carbohydrate concentration of a conventional steam-flaked corn-based growing finishing diet for Holstein steers can effectively enhance growth performance, particularly during the early growing and late finishing phases.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1400-1410
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• 2004

Eight crossbred (75% Holstein Friesian) cows in mid-lactation were randomly assigned to a switchback design with a 2x2 factorial arrangement to evaluate two nonstructural carbohydrate (NSC) sources (corn meal and cassava chips) with different rumen degradability and used at two levels of NSC (55 vs. 75%) with protein source (supplied by urea in the concentrate mix). The treatments were 1) Low degradable low level of corn (55%) 2) Low degradable high level of corn (75%) 3) High degradable low level of cassava (55%) and 4) High degradable high level of cassava (75%). The cows were offered the treatment concentrate at a ratio to milk yield at 1:2. Urea-treated rice straw was offered ad libitum as the roughage and supplement with 1 kg/hd/d cassava hay. The results revealed that total DM intake, BW and digestion coefficients of DM were not affected by either level or source of energy. Rumen fermentation parameters; NH3-N, blood urea nitrogen and milk urea nitrogen were unaffected by source of energy, but were dramatically increased by level of NSC. Rumen microorganism populations were not affected (p>0.05) by source of energy, but fungal zoospores were greater for cassava-based concentrate than corn-based concentrate. Milk production and milk composition were not affected significantly by diets containing either source or level of NSC, however concentrate than corn-based concentrate averaging (4.4 and 4.2, respectively). Likewise, income over feed, as estimated from 3.5% FCM, was higher on cassava-based concentrate than corn-based concentrate averaging (54.0 and 51.4 US$/mo, respectively). These results indicate that feeding diets containing either cassava-based diets and/or a higher of oncentrates up to 75% of DM with NPN (supplied by urea up to 4.5% of DM) can be used in dairy rations without altering rumen ecology or animal performance compared with corn-based concentrate.

• 한국작물학회지
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• 제30권2호
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• pp.131-139
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• 1985

이모작재배에 적합한 극조숙 양질 다수성 소맥품종육성을 위하여 등수기간에 관여하는 유전자 작용에 대한 연구는 매우 중요하다. 추파성품종 Yam-hill과 Hyslop, 춘파성품종 Red Bobs와 Siete Cellos를 사용하여 4 $\times$ 4 complete diallel crosses F$_1$, F$_2$, BC$_1$ 및 BC$_2$를 작성, Jinks-Hay-man model를 이용하여 등숙기간에 관여하는 유전자 작용을 분석하였다. 본 시험은 오레곤 주립대학교에서 실시되었다. 그 결과의 개요는 다음과 같다. 전등숙기간인 출수에서 생리적 성숙기까지의 기간과 출수에서 개화까지의 기간에서 maternal effect 가 인정되지 않았으며 비대립유전자간 상호작용도 발현되지 않았다. 개화에서 생리적 성숙기까지의 기간에서는 비대립유전자간에 상호작용이 인정되었다. 춘파성 품종인 Red Bobs와 Siete Cellos는 전등숙기간과 출수에서 개화까지의 기간을 지배하는 우성유전자들을 가지고 있었으며 짧은 쪽에 비하여 긴쪽이 우성으로 발현되고 있었다. 대조적으로 추파성 품종인 Yamhill 과 Hyslop은 개화에서 생리적 성숙기까지의 기간을 지배하는 우성유전자들을 가지고 있었으며 긴쪽이 우성으로 작용하고 있었다. 그리고 이 유전자들은 문배친들간에 독립적으로 분포되어 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.511-516
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• 2007

This experiment was conducted to evaluate the effects of differently maturing corn hybrids on silage production and milk production per unit area in the northern part of Japan, where grain development occurs under decreasing ambient temperature. Both hybrids were harvested at the same time. The stages of maturity for the early-maturing hybrids (EH; 80 d relative maturity) and the mid-maturing hybrids (MH; 93 d relative day) were early dent and late dough stage, respectively. The plant yields for MH were higher than those for EH. The dry matter (DM) content of MH was lower than that for EH, and the effluent loss for MH silage was greater than that for EH silage. Therefore, the DM yields of prepared silage per area were similar for both treatments. Twelve multiparous mid-lactation Holstein cows ($58{\pm}13$ days in milk) were fed diets based on EH or MH silage in a crossover design with two 3-week periods. Cows were fed 3 kg of hay crop silage (DM basis) and either EH or MH silage ad libitum, and concentrates were supplied to meet NRC requirement for dairy cows. Silage DM intake for EH was found to be higher (p<0.05) than that for MH (10.0 vs. 9.1 kg/day). Milk production and milk composition for EH were similar to those for MH. Feed efficiency per total feed intake was similar in both treatments, although the feed efficiency per concentrate intake tended to be higher for the EH than that for the MH diet. These results indicate that differences in maturation in corn hybrids affect the effluent production of silage and the silage intake of dairy cows. It may be advantageous to plant early hybrid corn with a reduction in effluent production of silage as well as a reduction in purchased feed costs for dairy cows under the climatic conditions of the northern part of Japan.

• Asian-Australasian Journal of Animal Sciences
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• 제24권8호
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• pp.1112-1119
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• 2011

The study aimed to evaluate the best level of licury oil in the diet of 3/4 Boer goats, as determined by profile analysis of commercial cuts on aspects of chemical composition, sensorial quality and fatty acid content. Nineteen male goats were used, with an initial weight of 10.8 kg/live weigh. The animals were fed with hay and a concentrated mix containing different levels of licury oil, which constituted the treatments. The experiment lasted for 60 days, at which point the animals were submitted to feed fasting and slaughtered. The carcass weight, commercial yield and cuts were measured. The ham was collected for sensorial and chemical evaluation and the longissimus dorsi was collected for fatty acid profile analysis. The addition of licury oil to the diet did not promote changes in the proportions and weights of the commercial cuts, nor to the meat's sensorial attributes. The sum of medium-chain fatty acids and the atherogenicity index was increased with the addition of oil. Licury oil can be added to the diet of goats (up to 4.5%) without resulting in changes in to the proportions of the commercial cuts, or to the chemical composition or sensorial characteristics of the meat. Based on the chain length of fatty acids, the addition of 4.5% licury oil can improve the quality of meat, but no effect was noted in relation to the atherogenicity index.

• 한국미생물·생명공학회지
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• 제17권3호
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• pp.224-230
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• 1989

Benzene으로부터 catechol을 생산하기 위하여 토양으로부터 benzene을 유일한 탄소원과 에너지원으로 이용할 수 있는 세균을 분리하였다. 분리된 균주들 중에서 benzene상에서 상대적 생육속도가 빠르며, catechol 생산능이 가장 좋은 균주인 KY-114를 선별하였고, Pseudomonas sp.로 부분 동정하였다. 이 균주에 의한 catechol 생산성을 증대시키기 위하여 돌연변이에 의한 균주 개발을 시도하였으며, 개발된 변이주인 Pseudomonas sp. HW-103을 이용하여 benzene으로부터 catechol 생산의 최적조건을 검토하였다. Catechol의 최대생산은 초기 pH6.5, 온도 3$0^{\circ}C$하에서 1% sodium citrate, 0.75%(NH$_4$)$_2$SO$_4$, 0.15% benzene과 다른 무기염이 함유된 배지에서 이루워졌으며, 이때 최대 catechol 생산량은 0.61g/ι 이었다. 한편, 휴지세포를 이용한 catechoι 생산을 위하여 4g/$\ell$의 benzene이 함유된 최적반응조건하에서 10시간 반응시킨 결과, 2.5g/$\ell$의 catechol이 생산되었으며, 이때의 이론적 전환률은 45%이었다.