• 한국가금학회지
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• 제46권2호
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• pp.77-86
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• 2019

본 연구는 겨울철에 발생하여 육성된 닭들과 여름철에 발생하여 육성된 닭들 간의 열 스트레스 반응 정도와 생산능력을 비교 분석하고자 하였다. 공시계로는 겨울철에 발생된 한국토종종계 초생추 1,156수와 여름철에 발생된 초생추 934수로 총 2,090수를 분석 대상으로 하였다. 스트레스 반응정도와 생산능력을 비교하기 위하여 텔로미어의 함량과 heat shock proteins(HSPs)의 유전자 발현율을 분석하고, 생존율, 산란율 및 체중을 조사하였다. 분석 결과, HSP-70, $HSP-90{\alpha}$ 및 $HSP-90{\beta}$ 유전자 발현율은 겨울철에 발생하여 육성된 닭들이 여름철에 발생하여 육성된 닭들에 비하여 모두 유의하게 높은 발현값을 나타내었다. 텔로미어 함량은 겨울철과 여름철에 발생한 닭들 간에 유의한 차이가 없었다. 생존율에서는 여름철 발생하여 육성된 닭들이 겨울철에 발생하여 육성된 닭들에 비해 유의하게 높았고, 산란율 및 난중 또한 여름철 발생 계군이 높게 나타났다. 반면, 초산일령은 겨울철 발생 계군이 여름철 발생 계군에 비해 빨랐다. 체중에 있어서 24주까지는 겨울철 발생 계군이 여름철 발생 계군에 비해 높았으나, 28주 이후부터 발생 계군 간 역전된 결과를 보였다. 결론적으로 여름철에 발생하여 육성된 닭들이 겨울철에 발생하여 육성된 닭들에 비해 열 스트레스에 대한 저항성이 높고 생산성이 우수함을 보였다. 이는 발생 및 육성 초기에 고온에 노출된 닭들이 상대적으로 높은 열적응성을 습득한 결과로 사료된다.

• 한국정보처리학회논문지
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• 제5권3호
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• pp.842-850
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• 1998

본 논문에서는 기계 도면의 분할 및 벡터화에 기반하여 해칭 영역을 인식하는 방법을 제안한다. 이러한 해칭영역의 인식은 다음의 세 단계로 구성된다. 먼저, 제안된 방법은 문자가 제거된 기계 도면으로부터 객체, 화살표 및 설명선(치수선, 해칭선등)의 분할 및 분리된 선분의 벡터화가 수행된다. 이러한 도면의 분할 및 벡터화가 수행되면 벡터화된 객체로부터 폐루프를 레이블링하여 행칭 영역의 후보를 결정한다. 마지막으로, 해칭 영역의 후보넹 포함되는 해칭선들을 검출함으로써 해칭 영역의 인식을 마루리한다. 제안된 방법에 의해 해칭 영역의 추출 및 인식이 용이함이 나타난다.

• Environmental Analysis Health and Toxicology
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• 제17권1호
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• pp.13-19
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• 2002

Effects of Formalin treatment on embryogenesis and larvae growing in fertilized eggs and hatched larvae of olive flounder, Paralichthys alivaceus were investigated. Fertilized eggs and hatched larvae were exposed to aqueous solution with 100, 200, 400, 800 and 1,000 ppm of nominal formalin concentration. They were kept in sea water of 16, 18 and 22$^{\circ}C$, respectively. Survival rate and hatchability of fertilized eggs were high in control groups more than formalin treated groups in 16, 18 and 22$^{\circ}C$, respectively (P<0.05). A similar effect that survival rate of hatched larvae was also observed for control and treatment groups. On the other hand, fertilized eggs treated with 400 ppm formalin, were all death in kept in 22$^{\circ}C$. These results suggest that high-dose of formalin in fertilized eggs and hatched larvae in olive flounder, Paralichthys olivaceus was inhibited the normal embryogenesis for fertilized eggs and growing for hatched larvae. Also, these inhibited effects was promoted in higher temperature.

• 한국패류학회지
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• 제32권2호
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• pp.133-139
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• 2016

The incubation time required for hatching of O. ocellatus were investigated through the processes of egg and embryonic developments by the dissecting microscopic and visual observations. And differences in ecological characteristics of the plankton mode of life or the benthic mode of life according to total numbers of the suckers on each short arm of the hatched juvenile larvae of O. ocellatus were studied by comparisons with other octopodidae species. Compared with the recent a few results reported by other researchers associated with the incubation time required for hatching by female adult mother of O. minor (73-90 days after spawning at $20.9-21.5^{\circ}C$ ranges), in this study, the incubation time required for hatching by female adult mother of O. ocellatus was 56-57 days after spawning at $11.0-20.4^{\circ}C$. Therefore, the incubation time required for hatching by female adult mother varied with Octopodidae species. In this studies, each ovarian egg laid by a female was connected to an egg string attaching to the surface of the wall or bottom of vacunt shell of Rapana venosa. Egg and embryonic developments of this species were studied in the indoor aquaria, in the specific gravity ranging 1.024-1.025. the hatched juvenile of O. ocellatus is 10.3 mm in the mean total length and 4.5 mm in mantle length, and each of its short arms has 18-20 suckers. The just hatched juvenile larvae of O. ocellatus enter the benthic mode of life (benthic larval stage) after hatching. In particular, regarding differences in ecological characteristics of the mode of life according to total numbers of the suckers, O. vulgaris may not need to have many suckers because they enter the planktonic mode of life after hatching, however O. ocellatus may need to have many suckers, because they should adapt to the benthic mode of life. And also the just hatched juvenile larvae of O. minor (bearing many suckers more than O. ocellatus) enter the benthic mode of life (benthic larval stage) after hatching. Therefore, the total number of the suckers on each short arm of the hatched juvenile larvae can be used for determining whether an octopus species has planktonic larval stages or benthic larval stage (benthic mode of life). In particular, The intracohort cannibalism phenomena appeared at the hatched juvenile larval stage because the larval stage of O. ocellatus and O. minor enter into the benthic larval stage in the early stage, unlike entering into the plaktonic larval stage in other Octopus species such as O. vulgaris: at this time, the early hatched larvae fed the late hatched larvae (they are the same species and almost same ages). Therefore, the intracohort cannibalism pheneomena occur in the just hatched juvenile stage of only O. ocellatus and O. minor.

• 설비공학논문집
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• 제14권8호
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• pp.641-647
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• 2002

Objectives of this paper are to investigate the effect of roughness of micro-hatching tubes on the absorption performance and to develop on experimental correlation of Nusselt as a function of the roughness. Three different micro-scale hatched tubes and a bare tube were tested in the present experiment. $H_{2}O/LiBr$ solution is used as working fluid. It was found that absorption performance of micro-scale hatched tubes were improved upto 2 times with an error band of ${\pm}25%$ compared with the bare tube. An experimental correlation of Nusselt was developed as a function of the roughness.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권2호
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• pp.107-110
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• 2001

Variations in protein and nucleic acid concentrations were observed in 24 hrs old eggs and hatched larvae of Nistari strain, Bombyx mori, exposed to starvation. Three starvation treatments of 24,48 and 60 hrs were given separately from 0 hr old fifth instar larvae. Biochemical variations were studied in the resultant hatched larvae of one time starved parent, while the eggs obtained from parents receiving starvation in two successive generations were considered for the study. In hatched larvae, protein levers in 24 hrs starvation groups remained significantly higher over control (never starved) while the same was found to be lower in 48 and 60 hrs starvation individuals. The RNA concentration remained significantly higher in all the treated lots. However, DNA content was not found to be significantly altered in hatched larvae after exposure to feeding stress. Protein, RNA and DNA concentration of 24 hrs old eggs produced by all the starved groups of Nistari, which had deceived two consecutive starvation during parental generations, showed higher concentrations of these biomolecules over control. Hence, starvation induced alterations in protein and nucleic acids in eggs and hatched Iarvae are indicative of a preparatory phase adopted by the insect to acclimatise itself and its progeny to stress situations.

• 한국수정란이식학회지
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• 제12권2호
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• pp.141-149
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• 1997

This study was carried out to investigate the effect of vitrification and slow freezing methods on the post-thaw developmental rate of rabbit zygotes. After exposing rabbit zygotes in EFS solution for 0.5, 1, 2, 3 and S min at room temperature, they were washed with 0.5 M sucrose solution, D-PBS and TCM-199 and then cultured in TCM-199 plus 10% FBS with bovine oviduct epithelial cells(BOEC) to examine whether the cryoprotectant induced injury during the various exposure periods. The embryo development rates to hatched blastocyst after exposing in EFS solution for 3 and 5 min(40.0 and 16.7%) were significantly lower than in 0.5, 1 and 2 min(63.0, 72.0 and 54.5%), respectively. The post-thaw development rates to hatched blastocyst were significantly(P<0.05) higher in in vivo morula with intact mucin coat(85.2%) and mucin seperated morula(77.8%) than those of in vitro morula(58.5%) and zygote(5.9%), hut no difference was shown between in vitro morulae and mucin separated morula. The cryoprotectant dilution procedures showed no effects on the post-thaw development rates to hatched blastocyst under the present culture conditions. The post-thaw development to hatched blastocyst in the rabbit zygotes was not significantly different between the slow freezing(12.8%) and vitrification(5.9%). These results indicated that the rabbit frozen zygotes could he successfully developed in vitro to hatched blastocysts, though their developmental rate was very low, compared with morula stage embryos, in either vitrification or slow freezing procedure under the present conditions.

• 한국응용곤충학회지
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• 제44권3호
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• pp.231-235
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• 2005

본 연구는 제주지역에서 계절초기 벚나무깍지벌레 방제적기를 구명하고자 월동성충의 산란시기 및 부화약충의 이동시기를 조사하였으며, 이 자료를 기존에 보고된 뽕나무깍지벌레 적산온도 예찰 모형에 적합하여 비교 검토하였다. 벚나무깍지벌레 암컷 월동성충은 4월 중하순부터 산란을 시작하여 5월 상순에는 그 알이 부화하기 시작하였으며, 5월 중순경 부화약충의 이동이 가장 활발하였다. 이와 같은 봄철 벚나무깍지벌레 발생시기는 우리나라 남부지역 및 일본 중부지역에서 보고된 뽕나무 깍지벌레 발생시기와 대체적으로 일치하였다. 또한 봄철 뽕나무깍지벌레의 >50% 부화 난괴 발생비율(y) 추정 적산온도 모형(y=1/[exp(-(-a+bx))];, a=-18.80, b=0.073; x=적산온도, 1월 1일부터 발육영점온도 $10.5^{\circ}C$ 적용)은 벚나무깍지벌레 부화약충 이동시기와 일치하여 봄철 벚나무깍지벌레 방제적기 추정에 유용하게 이용될 수 있을 것으로 판단되었다.

• The Korean Journal of Ecology
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• 제28권4호
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• pp.193-198
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• 2005

This study investigated the characteristics of the eggs and yolk sac larvae of Korean striped bitterling, Acheilognathus yamatsutae, spawned and grown In mussels. The number of eggs in the ovary was small ($358{\pm}108$ SD). The eggs were oval and large, and the formation of the perivitelline space was narrow. The eggs were hatched at only 41 hours after fertilization but the hatched larvae were underdeveloped. The development of yolk projection and minute tubercles on the skin surface was notable, along with the vividly moving tail in the hatched larvae. The yolk projection and minute tubercles were disappeared upon enhancement of the motor ability of the larvae was enhanced. The formation of eyes and body pigments of the larvae was relatively delayed in comparison with that of other cyprinid larvae. After completely consuming the yolks the larvae escaped from the mussel for free swimming and exogenous feeding.

• 한국잠사곤충학회지
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• 제10권
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• pp.59-62
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• 1969

암최청을 요약하면 대략 다음과 같다. 1. 춘잠기에는 암최청이 소잠율을 높인다. 따라서 일제소잠이 가능하며, 그 비율은 80-90％를 상회하지만 소잠이 일일간 연기된다. 2. 추잠기의 암최청은 효과는 다소 있으나 암환경에서 이미 의잠이 부화하므로 추잠기 이야포의 결과를 가져오니 하추잠기에는 암최청을 하지 않는 것이 생리적이라고 고찰한다.