• Title/Summary/Keyword: Hard surface

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In vitro maturation on a soft agarose matrix enhances the developmental ability of pig oocytes derived from small antral follicles

  • Park, Ji Eun;Lee, Seung Tae;Lee, Geun-Shik;Lee, Eunsong
    • Journal of Animal Reproduction and Biotechnology
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    • v.37 no.1
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    • pp.34-41
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    • 2022
  • In vivo oocytes grow and mature in ovarian follicles whereas oocytes are matured in vitro in plastic culture dishes with a hard surface. In vivo oocytes show a superior developmental ability to in vitro counterparts, indicating suboptimal environments of in vitro culture. This study aimed to evaluate the influence of an agarose matrix as a culture substrate during in vitro maturation (IVM) on the development of pig oocytes derived from small antral follicles (SAFs). Cumulus-oocyte complexes (COCs) retrieved from SAFs were grown in a plastic culture dish without an agarose matrix and then cultured for maturation in a plastic dish coated without (control) or with a 1% or 2% (w/v) agarose hydrogel. Then, the effect of the soft agarose matrix on oocyte maturation and embryonic development was assessed by analyzing intra-oocyte contents of glutathione (GSH) and reactive oxygen species (ROS), expression of VEGFA, HIF1A, and PFKP genes, and blastocyst formation after parthenogenesis. IVM of pig COCs on a 1% (w/v) agarose matrix showed a significantly higher blastocyst formation, intra-oocyte GSH contents, and transcript abundance of VEGFA. Moreover, a significantly lower intra-oocyte ROS content was detected in oocytes matured on the 1% and 2% (w/v) agarose matrices than in control. Our results demonstrated that IVM of SAFs-derived pig oocytes on a soft agarose matrix enhanced developmental ability by improving the cytoplasmic maturation of oocytes through redox balancing and regulation of gene expression.

Services of an Integrated Simulation Engine for Weapons Analysis (무기체계 효과도 분석을 위한 통합 모의 엔진의 서비스 구성 방안 연구)

  • Kim, Tae-Sup;Park, Joon-Ho;Kim, Hyun-Hwi;Park, Chan-Jong;Lee, Kang-Sun
    • Journal of the Korea Society for Simulation
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    • v.19 no.4
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    • pp.261-270
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    • 2010
  • An integrated simulation engine provides tools, services, and standards to support various activities in the entire M&S from modeling and simulation to analysis of the simulation results. Many countries have developed integrated simulation engines to efficiently assist complex M&S activities. However, we do not have domestic simulation engines especially designed for defense M&S, therefore, developing M&S softwares still remains as a hard task with high cost and tine. OpenSIM(Open Simulation engine for Interoperable Models) is an integrated simulation engine and provides tools, services and standard interfaces for weapons analysis. OpenSIM's services are comprised of classes, member functions and data attributes which are commonly used in modeling, simulating and analyzing weapons systems. In this paper, we introduce OpenSIM's services in C++ APIs and illustrate them through an ASM example(Air to Surface Missile).

User Expectation Experience of Flexible Display Interface (플렉서블 디스플레이 인터페이스의 사용자 기대경험)

  • Chung, Seung Eun;Yoon, Young Sun;Lee, Ram;Lim, Yeon Sun;Choi, Ho Jeong;Ryoo, Han Young
    • Design Convergence Study
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    • v.15 no.2
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    • pp.301-317
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    • 2016
  • Flexible display interface is capable of creating new user behaviors based on its characteristics of outstanding surface representation and display transformations such as bending, rolling, and folding. Thus, it is being discussed that the newly emerging flexible display interface can offer a different user experience that the previous flat display couldn't. However, as it is hard to find studies that identify the general attributes of user experience in the area of flexible display research, this study was intended to identify and label experience that users expect in a flexible display interface. For this purpose, this study first investigated literature reviews about user experience in previous digital media interface and flexible display interface and conducted interview research in order to reflect the users' perception, collecting 52 items that represent user experience. In addition, these items were used as measurements to deduct different types of user experience, and 308 interviewees participated in the interview research process. As a result, 10 types of user experience were developed from the results of the survey: functionality, understandability, pleasure, convenience, familiarity, stimulation, adaptability, collectivity, reality, and aesthetic.

A Study on the Detection of Marine Debris in Collection Blind Spots using Drones and a Method for Matching Latitude and Longitude (드론을 활용한 수거사각지대 해양쓰레기 탐지 및 위경도 매칭 방법에 관한 연구)

  • Sang-Hyun Ha;Eun-Sung Choi;Ji Yeon Kim;Sung-Hoon Oh;Seok Chan Jeong
    • The Journal of Bigdata
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    • v.8 no.1
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    • pp.73-82
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    • 2023
  • Marine debris not only affects the survival of marine life, water pollution, and scenery but also has secondary effects on economic loss and human health. While research on underwater and surface debris is actively ongoing, solutions to marine debris in hard-to-reach blind spots are being developed slowly. To address this problem, we utilize drones to detect and track marine debris in blind spots such as tetrapods. The detected debris is then visualized by calculating its location coordinates using the drone's GPS, altitude, and heading values. The proposed method of using drones for detecting marine debris and matching it with longitude and latitude coordinates provides an effective solution to the problem of marine debris in blind spots.

Time Integration Algorithm for the Estimation of Daily Primary Production (식물플랑크톤 일차생산력의 새로운 시간 적분 알고리즘)

  • Park, Jong-Gyu;Kim, Eung-Kwon
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.15 no.3
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    • pp.124-132
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    • 2010
  • In spite of the global importance of primary production of phytoplankton, some primary production data in Korean coastal waters still need to be better processed. The daily rates of water column primary production is generally estimated by integrating the primary production per unit volume over time and depth, but efforts for time integration algorithm have been conducted insufficiently. In this study a mathematical equation evaluating daily primary production integrated over time of a day is proposed and the effectiveness of the model is tested on Saemangeum Lake. The daily primary productions computed with the proposed equation were nearly the same with the results numerically integrated by substituting solar irradiance data. It was suggested that better estimation of primary production would be obtained by using monthly or weekly means of solar irradiance rather than more variable daily data. Because of the vertically heterogenous distribution of phytoplankton, it's hard to integrate the equation over depth to give the daily rates of primary production per unit area of water surface. However, the problem would be solved if, after the vertical distribution of phytoplankton was classified into several patterns and reduced to mathematical formula, every composite function of time integrated equation and chlorophyll distribution equation was integrated successfully.

Manufacturing and Characterization of Organic-Inorganic Hybrid Coating Film Using Sol-Gel Method (Sol-Gel법을 이용한 유무기 하이브리드 코팅막 제조 및 특성평가)

  • Seungwon Cho;Dabin Kim;Ji-Sun Lee;Dongwook Shin;Jinho Kim
    • Korean Journal of Materials Research
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    • v.34 no.9
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    • pp.439-447
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    • 2024
  • Organic-inorganic hybrid coating films have been used to increase the transmittance and enhance the physical properties of plastic substrates. Sol-gel organic-inorganic thin films were fabricated on polymethylmethacrylate (PMMA) substrates using a dip coater. Metal alkoxide precursor tetraethylsilicate (TEOS) and alkoxy silanes including decyltrimethoxysilane (DTMS), 3-glycidoxypropyltrimethoxysilane (GPTMS), phenyltrimethoxysilane (PTMS), 3-(trimethoxysilyl)propyl methacrylate (TMSPM) and vinyltrimethoxysilane (VTMS) were used to synthesize sol-gel hybrid coating solutions. Sol-gel synthesis was confirmed by the results of FT-IR. Cross-linking of the Si-O-Si network during synthesis of the sol-gel reaction was confirmed. The effects of each alkoxy silane on the coating film properties were investigated. All of the organic-inorganic hybrid coatings showed improved transmittance of over 90 %. The surface hardness of all coating films on the PMMA substrate was measured to be 4H or higher and the average thickness of the coating films was measured to be about 500 nm. Notably, the TEOS/DTMS coating film showed excellent hydrophobic properties, of about 97°.

MULTISTEP HEAT-TREATMENT EFFECTS ON ELECTROSPUN Nd-Fe-B-O NANOFIBERS

  • EUN JU JEON;NU SI A. EOM;JIMIN LEE;BIN LEE;HYE MI CHO;JI SUN ON;YONG-HO CHOA ;BUM SUNG KIM
    • Archives of Metallurgy and Materials
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    • v.63 no.3
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    • pp.1433-1437
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    • 2018
  • Neodymium-Iron-Boron (Nd-Fe-B) magnets are considered to have the highest energy density, and their applications include electric motors, generators, hard disc drives, and MRI. It is well known that a fiber structure with a high aspect ratio and the large specific surface area has the potential to overcome the limitations, such as inhomogeneous structures and the difficulty in alignment of easy axis, associated with such magnets obtained by conventional methods. In this work, a suitable heat-treatment procedure based on single-step and multistep treatments to synthesize sound electrospun Nd-Fe-B-O nanofibers of Φ572 nm was investigated. The single-step heat-treated (directly heat-treated at 800℃ for 2 h in air) samples disintegrated along with the residual organic compounds, whereas the multistep heat-treated (sequential three-step heat-treated including three steps;: dehydration (250℃ for 30 min in an inert atmosphere), debinding (650℃ for 30 min in air), and calcination (800℃ for 1 h in air)) fibers maintained sound fibrous morphology without any organic impurities. They could maintain such fibrous morphologies during the dehydration and debinding steps because of the relatively low internal pressures of water vapor and polymer, respectively. In addition, the NdFeO3 alloying phase was dominant in the multistep heat-treated fibers due to the removal of barriers to mass transfer in the interparticles.

The Comparative Study on the wettability of the RGP lens multi-purpose solutions(MPSs) (RGP lens 다목적용액의 습윤성에 관한 비교 연구)

  • Park, Hyun-Ju;Goe, Eun-Kyung;Seo, Eun-Sun;Kim, Jai-Min;Lee, Ki-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.8 no.2
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    • pp.119-127
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    • 2003
  • We measured the effect of wettability of six MPSs for RGP lens. The used MPSs(multipurpose solutions) were OPTI-SOAK(ALCON), SOLO care hard(CIBA Vision), Total care(ALLERGAN), Simplicity(BOSTON). Wetting and Soaking Sol.(Bausch & Lomb) and Aquas-multi(Saehan). These MPSs keeps hydrophilic property of lens surface and increase the effect of cleaning or increase the effect of preservative effect. To compare with the effect of wettability we followed the way of contact angle measurement which was general way to measure wettability and compared lens which was conducted by each MPS made by different companies. As a control, 0.9% NaCl solution and artificial tears were used. The degree of the effect of wettability was decided by contact angle. It is hydrophilic property nearby $0^{\circ}$ of contact angle and it is closed by hydrophobic property as it increases. To compare with the effect of wettability we followed the way of contact angle measurement which was general way to measure wettability and compared lens which was conducted by each MPS made by different companies. As a control, 0.9% NaCl solution and artificial tears were used. The degree of the effect of wettability was decided by contact angle. It is hydrophilic property nearby $0^{\circ}$ of contact angle and it is closed by hydrophobic property as it increases. The results showed that every lens was nearby hydrophilic property within $25^{\circ}{\sim}36^{\circ}$. Also, it was differed by various factors. The surface tension showed various differences between 19.8 and 31.3(mN/m). In the viscosity, MPSs represented the highest viscosity between $4^{\circ}C$ and $20^{\circ}C$. It was much higher than compared with the viscosity of soft lens MPS. This experiment could be used to grasping the interaction between solutions used to MPS and the natural endowments of lens and to considering the relations of different factors effecting the wettability.

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Trend in Research and Application of Hard Carbon-based Thin Films (탄소계 경질 박막의 연구 및 산업 적용 동향)

  • Lee, Gyeong-Hwang;Park, Jong-Won;Yang, Ji-Hun;Jeong, Jae-In
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2009.05a
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    • pp.111-112
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    • 2009
  • Diamond-like carbon (DLC) is a convenient term to indicate the compositions of the various forms of amorphous carbon (a-C), tetrahedral amorphous carbon (ta-C), hydrogenated amorphous carbon and tetrahedral amorphous carbon (a-C:H and ta-C:H). The a-C film with disordered graphitic ordering, such as soot, chars, glassy carbon, and evaporated a-C, is shown in the lower left hand corner. If the fraction of sp3 bonding reaches a high degree, such an a-C is denoted as tetrahedral amorphous carbon (ta-C), in order to distinguish it from sp2 a-C [2]. Two hydrocarbon polymers, that is, polyethylene (CH2)n and polyacetylene (CH)n, define the limits of the triangle in the right hand corner beyond which interconnecting C-C networks do not form, and only strait-chain molecules are formed. The DLC films, i.e. a-C, ta-C, a-C:H and ta-C:H, have some extreme properties similar to diamond, such as hardness, elastic modulus and chemical inertness. These films are great advantages for many applications. One of the most important applications of the carbon-based films is the coating for magnetic hard disk recording. The second successful application is wear protective and antireflective films for IR windows. The third application is wear protection of bearings and sliding friction parts. The fourth is precision gages for the automotive industry. Recently, exciting ongoing study [1] tries to deposit a carbon-based protective film on engine parts (e.g. engine cylinders and pistons) taking into account not only low friction and wear, but also self lubricating properties. Reduction of the oil consumption is expected. Currently, for an additional application field, the carbon-based films are extensively studied as excellent candidates for biocompatible films on biomedical implants. The carbon-based films consist of carbon, hydrogen and nitrogen, which are biologically harmless as well as the main elements of human body. Some in vitro and limited in vivo studies on the biological effects of carbon-based films have been studied [$2{\sim}5$].The carbon-based films have great potentials in many fields. However, a few technological issues for carbon-based film are still needed to be studied to improve the applicability. Aisenberg and Chabot [3] firstly prepared an amorphous carbon film on substrates remained at room temperature using a beam of carbon ions produced using argon plasma. Spencer et al. [4] had subsequently developed this field. Many deposition techniques for DLC films have been developed to increase the fraction of sp3 bonding in the films. The a-C films have been prepared by a variety of deposition methods such as ion plating, DC or RF sputtering, RF or DC plasma enhanced chemical vapor deposition (PECVD), electron cyclotron resonance chemical vapor deposition (ECR-CVD), ion implantation, ablation, pulsed laser deposition and cathodic arc deposition, from a variety of carbon target or gaseous sources materials [5]. Sputtering is the most common deposition method for a-C film. Deposited films by these plasma methods, such as plasma enhanced chemical vapor deposition (PECVD) [6], are ranged into the interior of the triangle. Application fields of DLC films investigated from papers. Many papers purposed to apply for tribology due to the carbon-based films of low friction and wear resistance. Figure 1 shows the percentage of DLC research interest for application field. The biggest portion is tribology field. It is occupied 57%. Second, biomedical field hold 14%. Nowadays, biomedical field is took notice in many countries and significantly increased the research papers. DLC films actually applied to many industries in 2005 as shown figure 2. The most applied fields are mold and machinery industries. It took over 50%. The automobile industry is more and more increase application parts. In the near future, automobile industry is expected a big market for DLC coating. Figure 1 Research interests of carbon-based filmsFigure 2 Demand ratio of DLC coating for industry in 2005. In this presentation, I will introduce a trend of carbon-based coating research and applications.

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Development of System for Real-Time Object Recognition and Matching using Deep Learning at Simulated Lunar Surface Environment (딥러닝 기반 달 표면 모사 환경 실시간 객체 인식 및 매칭 시스템 개발)

  • Jong-Ho Na;Jun-Ho Gong;Su-Deuk Lee;Hyu-Soung Shin
    • Tunnel and Underground Space
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    • v.33 no.4
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    • pp.281-298
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    • 2023
  • Continuous research efforts are being devoted to unmanned mobile platforms for lunar exploration. There is an ongoing demand for real-time information processing to accurately determine the positioning and mapping of areas of interest on the lunar surface. To apply deep learning processing and analysis techniques to practical rovers, research on software integration and optimization is imperative. In this study, a foundational investigation has been conducted on real-time analysis of virtual lunar base construction site images, aimed at automatically quantifying spatial information of key objects. This study involved transitioning from an existing region-based object recognition algorithm to a boundary box-based algorithm, thus enhancing object recognition accuracy and inference speed. To facilitate extensive data-based object matching training, the Batch Hard Triplet Mining technique was introduced, and research was conducted to optimize both training and inference processes. Furthermore, an improved software system for object recognition and identical object matching was integrated, accompanied by the development of visualization software for the automatic matching of identical objects within input images. Leveraging satellite simulative captured video data for training objects and moving object-captured video data for inference, training and inference for identical object matching were successfully executed. The outcomes of this research suggest the feasibility of implementing 3D spatial information based on continuous-capture video data of mobile platforms and utilizing it for positioning objects within regions of interest. As a result, these findings are expected to contribute to the integration of an automated on-site system for video-based construction monitoring and control of significant target objects within future lunar base construction sites.