• 한국수정란이식학회지
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• 제33권4호
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• pp.265-270
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• 2018

Up-to-date artificial insemination (AI) using frozen sperm consider as the most widely using technology for improvement of Korean Native Cow (Hanwoo) embryo production. However, it is time consuming, required at least 15~20 years to make more than 6 generations, and their offspring number is limited. To overcome such limitations, superovulation and in vitro fertilization have been developed. For superovulation, the number of produced embryos are not enough for commercialization and donor cows need rest period. This led to use of slaughterhouse ovary for in vitro fertilization, but it is impossible to repeat the collection from the same individual and it only can improve the genetic merits of offspring for one generation. Production of embryos using Ovum Pick-Up (OPU) technique, where oocytes can be repeatedly collected from living elite donor, might overcome these limitations. In this study, we investigated the possibility of using OPU technique from donors at different age and different session periods for mass-embryo-production. Oocytes were collected from 26 donor cows twice per week, 3 - 4 months per year, between 2013 and 2016. Results showed that, the average number of embryo produced in first year used donor was significantly higher than that in second year used donor ($3.89{\pm}2.85$ vs $3.29{\pm}2.70$), however, there was no significant difference between third year used donor ($3.51{\pm}3.32$) and other groups. Taken together, our data showed that repeated using of donor up to three years is possible for in vitro embryo mass-production. Moreover, OPU can be used as suitable embryo producing technique for livestock breed improvement.

• 농업과학연구
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• 제48권3호
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• pp.557-565
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• 2021

Interest in research on various medicinal plants has increased globally over the last few decades, possibly due to their possible antibacterial and antioxidant activities. The present study was conducted to verify the antioxidant effects, antibacterial activity, and collagen synthesis and cell viability outcomes of adipocytes upon exposure to Abeliophyllum distichum Nakai (AdN). Antibacterial activity was measured through the Disc diffusion method to compare the growth ability of pathogenic microorganisms (E.coli, Salmonella). The absorbance was measured at 560 nm to calculate the active oxygen scavenging ability. Fibroblasts were dispensed in a 96-well plate at a density of 1 × 10⁵ cells·well^-1. The amount of procollagen was measured in each case using a procollagen type 1 C-peptide EIA KIT. The cytotoxicity of the Abeliophyllum distichum Nakai extract against animal adipocytes (Hanwoo backfat cells) was determined using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, a method that measures the conversion of MTS to Formazan by means of mitochondrial dehydrogenases. The concentrations of the samples were made to be 0.0125, 0025, 0.05, 0.1, and 0.5% and all were -completely absorbed into the disc in an incubator at 37℃ for 24 to 36 hours. For the 0.125 mg·disc^-1, effects of Abeliophyllum distichum Nakai on the antioxidant effect, antibacterial activity, and cell viability of adipocytes were found. However, Abeliophyllum distichum Nakai had no effect on collagen synthesis, thus suggesting that AdN extracts may be useful for the prevention and/or treatment of obesity.

• 농업과학연구
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• 제48권2호
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• pp.333-342
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• 2021

This study attempted to compare total digestible nutrients (TDN) calculated as Rohweder, NRC, and Waldo and Peiqiang methods and TDN measured as digestibility of in vivo appearance. Rohweder method showed that the TDN of rice straw and timothy were 54.32% and 57.79%, respectively. In NRC method, the digestibility of neutral detergent fiber (NDF) and TDN of rice straw were 50.76% and 53.15%, respectively. When NRC method was applied in Timothy, the digestibility of NDF and TDN were 51.53% and 55.22%, respectively. Waldo and Peiqiang method calculated the results through the rumen in situ test. NDF digestibility of rice straw and timothy was 44.61% and 51.82%, which was different from the results of NRC method. In addition, TDN was predicted to be 48.85% for rice straw and 55.41% for timothy. In the in vivo apparent digestibility experiment, the digestibility of NDF and TDN in rice straw was 41.10% and 44.79%, respectively. In timothy, the digestibility of NDF and TDN were measured as 51.29% and 58.18%, respectively. As a result of a series of studies, rice straw was found in Rohweder and NRC methods showed higher TDN than other methods. In this study, there was a difference in rice straw by measurement method, but there was no difference in timothy. Therefore, when evaluating the value of feed in order to provide roughage to ruminant, calculation methods must be modified and supplemented. In addition, TDN should be considered to apply several evaluation methods instead of one method.

• Journal of Veterinary Science
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• 제23권6호
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• pp.85.1-85.11
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• 2022

Background: Neonatal calf diarrhea is a major problem in the cattle industry worldwide. Rotavirus and Cryptosporidium parvum are the primary causative agents, especially during the first three weeks of the calf's life. Objectives: This study investigated the differences in acid-base, electrolytes, and biochemical parameters of diarrheic calves with infection of either rotavirus or C. parvum. Methods: A total of 61 Korean native calves (≤ 20 days old) were divided into two groups based on rotavirus or C. parvum infections: rotavirus infection (n = 44) and C. parvum infection (n = 17). The calves with at a specific blood pH range (pH 6.92-7.25) were chosen for comparison. The acid-base, electrolyte, chemistry, and serum proteins were analyzed, Further, fecal examinations were performed. Results: Compared to C. parvum-infected calves, the rotavirus-infected calves showed lower levels of total carbon dioxide, bicarbonate (HCO₃^-), anion gap, total protein, and albumin/globulin ratio, and significantly lower levels of potassium, globulin, and α2-globulin (p < 0.05). The C. parvum-infected calves (r = 0.749) had stronger correlations between pH and HCO₃^- than the rotavirus-infected calves (r = 0.598). Compared to rotavirus-infected calves, strong correlations between globulin and α2-globulin, α2-globulin and haptoglobin were identified in C. parvum-infected calves. Conclusions: This study is the first to investigate acid-base, electrolyte, and biochemical parameters in calves in response to infections of rotavirus and C. parvum. Although rotavirus and C. parvum cause malabsorptive and secretory diarrhea in similar-aged calves, blood parameters were different. This would help establish the diagnostic and treatment strategies.

• 한국축산식품학회지
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• 제43권1호
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• pp.85-100
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• 2023

The present study aimed to evaluate the effects of high-intensity ultrasound (HIU) application on meat quality traits, sensory parameters, and the microstructure of semitendinosus muscle from Hanwoo cattle. The samples were treated in an ultrasonic bath (35 kHz) at an intensity of 800 W/cm² for 60 min, followed by aging at 1℃ for 0, 3, and 7 days. The application of ultrasound resulted in lower Warner-Bratzler shear force and higher myofibrillar fragmentation index values during the storage period. HIU also enhanced the tenderness, flavor, umami, and overall acceptability of cooked beef muscle. However, the electronic tongue evaluation results showed higher umami values in the control treatment on the seventh day of storage. The microstructure of sonicated meat showed disorganized myofibrillar architecture and swelling in the A-band region of sarcomeres during the storage period, which led to greater meat tenderness. The heatmap illustrated the high abundance of α-linolenic acid (C20:5n3) and eicosapentaenoic acid (C18:3n3) in sonicated meat samples on the third day of the storage. These results showed that HIU is a potential method for tenderizing and improving the sensory attributes of beef without compromising other quality aspects.

• 한국축산식품학회지
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• 제42권2호
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• pp.240-251
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• 2022

Fat deposition in animal muscles differs according to the genetics and muscle anatomical locations. Moreover, different fat to lean muscle ratios (quality grade, QG) might contribute to aroma development in highly marbled beef. Scientific evidence is required to determine whether the abundance of aroma volatiles is positively correlated with the amount of fat in highly marbled beef. Therefore, this study aims to investigate the effect of QG on beef aroma profile using electronic nose data and a chemometric approach. An electronic nose with metal oxide semiconductors was used, and discrimination was performed using multivariate analysis, including principal component analysis and hierarchical clustering. The M. longissimus lumborum (striploin) of QG 1++, 1+, 1, and 2 of Hanwoo steers (n=6), finished under identical feeding systems on similar farms, were used. In contrast to the proportion of monounsaturated fatty acids (MUFAs), the abundance of volatile compounds and the proportion of polyunsaturated fatty acids (PUFAs) decreased as the QG increased. The aroma profile of striploin from carcasses of different QGs was well-discriminated. QG1++ was close to QG1+, while QG1 and QG2 were within a cluster. In conclusion, aroma development in beef is strongly influenced by fat deposition, particularly the fat-to-lean muscle ratio with regard to the proportion of PUFA. As MUFA slows down the oxidation and release of volatile compounds, leaner beef containing a higher proportion of PUFA produces more volatile compounds than beef with a higher amount of intramuscular fat.

• 한국임상수의학회지
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• 제40권3호
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• pp.215-220
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• 2023

A 3-month-old Korean native cattle (Hanwoo) calf with difficulty taking normal posture and an inability to rise was referred for a definite diagnosis and active treatment, including surgery. The calf had a history of an accident in which both hind limbs were trapped in a barn structure. After admission, a "rabbit leg" posture was observed, a typical sign of gastrocnemius muscle rupture, and both digits were knuckled downward like they were trying to grip the ground. This was considered to be a result of the superficial digital flexor not rupturing but only the gastrocnemius muscle rupturing. Physical examination revealed laceration of the metatarsus and firmness behind both stifle joints which were presumed to be the sites of gastrocnemius muscle rupture. Skeletal abnormalities, including fractures, were ruled out by radiography. Based on these findings, the patient was diagnosed with bilateral gastrocnemius muscle rupture, and surgery was performed to reconnect the head of the ruptured muscle. Because the rupture occurred perpendicular to the muscle direction, the locking loop technique, a method of suturing severed tendons, was used to reduce the tension. After surgery, the cast was used to prevent further injuries and promote voluntary rehabilitation. Follow-up was completed, with the calf showing normal posture and gait 112 days after surgery. This is the first case report in the Republic of Korea describing the successful diagnosis and treatment of bilateral gastrocnemius muscle rupture in a calf.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2023년도 임시총회 및 춘계학술대회
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• pp.4-4
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• 2023

다국적 제약회사에서 생산되는 제약화합물은 인간의 질병치료 뿐만 아니라 가축의 생산성을 향상시키는 물질으로도 활발하게 생산되는데 육우의 성장을 촉진하는 기능성 화합물들도 많이 있다. 북미에서 생산되는 소고기의 약 80%가 1번 이상의 성장촉진기술들이 활용되고 있다고 보고된다. 고기소로 생산되는 비육우에 적용되는 방법은 크게 피하 이식에 의해 혈류를 타고 성장을 촉진하는 17β-Estradiol과 합성 남성호르몬제인 Trenbolone acetate가 주로 활용되고, 비육후기 사료에 섞어서 급여하는 사료첨가제 형태인 β2-adrenergic agonist 같은 형태로 적용하게 된다. 근육을 성장하는 기술의 작용기전은 많은 선행연구에 의해 밝혀져 있는 반면 천연 알칼로이드 성분들의 기전은 밝혀진 것이 많지 않다. 한방제재들에서 많이 발견되는 알칼로이드 성분들은 생리활성 기능들을 가진 것으로 알려져 있지만, 생산, 수거, 가공, 추출 등의 공정에서 많은 비용이 발생하므로 비육우의 사료화 가능성은 아주 희박하다. 따라서 비용을 최소화 할 수 있는 후보재료를 검색중에 감자부산물을 확보하였고 기능성 물질의 추출과 사료첨가제 화 하여 비육우에 급여시험을 실시하였다. α-solanine과 α-chaconine은 감자의 잎, 과일 및 괴경에서 발견되는 글리코알칼로이드 화합물로, 쥐, 토끼, 닭과 같은 다양한 동물 모델에서 중독성을 가진 물질로 보고 되고 있다. 최근 연구에서는 비육우의 성능을 유도하는 데 사용되는 것으로 나타났다. 한우 송아지 3마리의 사태(Semimembranosus)와 등심(Longissimus Dorci)근육에서 추출된 근육위성 세포(BSC)에 다양한 수준의 α-solanine(control, 0.001, 0.01, 0.1, 1, 10μM)으로 처리해본 결과 근육관련 지표인 MHC2X과 β2-AR의 발현이 높게 나타난 것을 확인했다. 사료급여실험에서는 대조군에 비해 급여군의 등심단면적과 도체중이 향상되는 것을 확인하였다. 결과적으로 감자유래 농산부산물은 한우 비육우의 근육의 성장을 증가시키고, 그 작용기전은 β2-수용체에 작용하여 단백질 합성을 촉진시켜 근육을 축적시키는 것으로 확인하였다. 농산부산물을 이용한 기능성 사료개발은 최근 이슈가 되고 있는 축산분야 탄소저감을 개선할 수 있는 기술로 축산의 업사이클링 기술로 활용 가능하다.

• 한국가축번식학회지
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• 제23권2호
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• pp.175-180
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• 1999

본 실험은 한우 번식장애우와 저수태우의 혈액 성분 및 성선호르몬(estrogen과 progesterone)의 농도를 비교 분석하여 번식우의 기초적인 생리지표를 확립시키는데 있다. 본 실험의 결과를 요약하면 다음과 같다. 1. 저수태 한우와 번식장애우 혈중 albumin, BUN, Ca, cholesterol, creatine, glucose, phosphorous, total protein 및 triglyceride 함량은 3.28$\pm$0.05 와 3.27$\pm$0.04 g/㎗, 11.07$\pm$0.61와 12.69$\pm$0.88 mg/㎗, 9.98$\pm$0.10와 9.56$\pm$0.11mg/㎗, 105.75$\pm$3.57와 126.78$\pm$5.66 mg/㎗, 1.62$\pm$0.05와 1.65$\pm$0.09 mg/㎗, 67.46$\pm$3.07와 76.97$\pm$3.76 mg/㎗, 6.13$\pm$0.36 와 6.11$\pm$0.26 mg/㎗, 5.82$\pm$0.08와 6.18$\pm$0.08 g/㎗ 및 12.82$\pm$1.46와 15.19$\pm$1.61 mg/㎗으로 나타났다. 2. 혈청의 중요한 성분중 하나인 요소태 질소, 칼슘 및 인의 함량이 번식 장애우와 저수태우의 경우 정상우보다 다소 높은 경향을 나타냈으며, 특히 콜레스테롤의 함량이 번식 장애우와 저수태우의 경우 정상우의 약 2배정도 현저히 높은 성적을 나타냈다. 3. 발정주기중 혈액내 progesterone 수준은 임신우와 비임신우에서 수정후 18일까지는 비슷한 수준(6.55$\pm$0.17, 5.46$\pm$0.18 ng/$m\ell$)을 보였으나 그 후 임신우에서는 점차 증가하였고 비임신우에서는 발정일 (0.18$\pm$0.03 ng/$m\ell$)에 급감하였다. 4. 번식 장애우와 저수태우의 혈중 estrogen과 progesterone 함량은 각각 57.42$\pm$5.03, 56.76$\pm$8.73 pg/$m\ell$과 6.26$\pm$0.83 ng/$m\ell$, 9.41$\pm$1.85 ng/$m\ell$으로서, 혈중 progesterone 농도가 정상우의 황체기 수준으로 유지되어 발정발현과 수태가 억제되는 것으로 나타났다.

• 한국수정란이식학회지
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• 제20권1호
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• pp.55-62
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• 2005

도축장에서 회수한 한우 난소로부터 난자를 회수하기 위한 방법으로 흡입법 후 세절법과 흡입법으로 난자를 회수하여, 난자의 회수율과 채란된 난자를 체외수정 후 발달율과 수정란 이식 후 수태율에 영향을 조사한 결과는 다음과 같다. 1. 난자 회수율은 각 난소당 회수된 난자수는 흡입 후 세절법이 8.2개, 흡입법이 6.5개로서 흡입 후 세절법을 병용하는 것이 난자 회수율에서 유의적으로 많았다. 2. 채란방법에 따른 체외수정란의 분할율은 흡입 후 세절법이 $75.8\%$, 흡입법은 $84.4\%$로서 유의적이 차이를 보이지 않았다. 3. 배반포 발달율은 흡입 후 세절법이 $28.3\%$, 흡입법은 $22.8\%$로서 흡입 후 세절법이 유의적으로 높았다. 4. 난소당 배반포수에서는 흡입 후 세절법이 1.8개로서 흡입법의 1.1개보다 유의적으로 많은 배반포수를 생산할 수 있었다. 5. 채란별 수태율 조사 결과 흡입 후 세절법이 $54.4\%$, 흡입법이 $62.5\%$로서 흡입법으로 채란된 난자로부터 얻어진 수정란을 이식하였을 때 높은 수태율을 얻을 수 있었다. 6. 경산우와 처녀우에 수정란이식 후 수태율은 경산우는 흡입법 후 세절법이 $54.4\%$, 흡입법은 $62.5\%$ 나타났고, 처녀우는 흡입법 후 세절법이 $58.1\%$, 흡입법은 $68.2\%$로서 경산우와 처녀우에 관계없이 흡입법으로 채란한 난자로부터 생산된 수정란을 이식하였을 때 수태율이 유의적으로 높은 결과를 얻었다. 이상의 결과에서 채란방법에 따른 난소당 이용 가능한 난자의 회수율은 흡입 후 세절법을 이용함으로서 많은 난자와 수정란을 생산할 수 있었다. 그리하여 한정된 난소를 효율적으로 활용하기 위해서는 흡입 후 세절법을 이용하는 것이 전체적인 효율 향상에 도움이 될 것으로 판단된다.