• Title, Summary, Keyword: Hanwoo

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Development of In Vitro Culture System for Male Germline Stem Cells in Porcine (돼지 웅성 생식선 줄기세포의 체외배양기법 개발)

  • Kim, Yong-Hee;Kim, Byung-Gak;Lee, Yong-An;Kim, Bang-Jin;Kim, Ki-Jung;Lee, Myeung-Sik;Im, Gi-Sun;Ryu, Buom-Yong
    • Reproductive and Developmental Biology
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    • v.33 no.3
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    • pp.171-177
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    • 2009
  • Spermatogonial stem cells(SSCs) only are responsible for the generation of progeny and for the transmission of genetic information to the next generation in male. Other in vitro studies have cultured SSCs for proliferation, differentiation, and genetic modification in mouse and rat. Currently, information regarding in vitro culture of porcine Germline Stem Cell(GSC) such as gonocyte or SSC is limited and is in need of further studies. Therefore, in this study, we report development of a successful culture system for gonocytes of neonatal porcine testes. Testis cells were extracted from $10{\sim}14$-day-old pigs. These cells were harvested using enzymatic digestion, and the harvested cells were purified with combination of percoll, laminin, and gelatin selection techniques. The most effective culture system of porcine gonocytes was established through trial experiments which made a comparison between different feeder cells, medium, serum concentrations, temperatures, and $O_2$ tensions. Taken together, the optimal condition was established using C166 or Mouse Embryonic Fibroblast(MEF) feeder cell, Rat Serum Free Medium(RSFM), 0% serum concentration, $37^{\circ}C$ temperature, and $O_2$ 20% tension. Although we discovered the optimal culture condition for proliferation of porcine gonocytes, the gonocyte colonies ceased to expand after one month. These results suggest inadequate acquirement of ingredients essential for long term culture of porcine GSCs. Consequently, further study should be conducted to establish a successful long-term culture system for porcine GSCs by introducing various growth factors or nutrients.

Effect of Gender-specific Bovine Serum Supplemented Medium on Cell Culture (성별 특이 소 혈청이 세포 배양에 미치는 영향)

  • Lee, Dong-Mok;Choi, Moon-Seok;Woo, Gyung-Il;Shin, Yu-Mi;Lee, Ki-Ho;Cheon, Yong-Pil;Chun, Tae-Hoon;Choi, In-Ho
    • Journal of Animal Science and Technology
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    • v.51 no.5
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    • pp.413-420
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    • 2009
  • The research has been aimed to investigate the effect of different sera including fetal bovine serum (FBS), male bovine serum (MS), female bovine serum (FS), and castrated-male bovine serum (C-MS) on cell proliferation, follicular maturation and ovulation in vitro. Established cell lines and primary cells were cultured in the culture media supplemented with different sera and cells proliferation was observed by cell counting and MTT assay. The results indicated that cell proliferation was significantly different for different serum source. Proliferation of bovine and human myogenic satellite cells was highest in MS. In contrast, proliferation of breast cancer cells and immune cells were the highest in FS and FBS, respectively. There was no difference in the rate of follicular growth, whereas the rate of ovulation was higher in FBS and C-MS. Finally, the wound healing effect and cell proliferation of 3T3-L1 cells showed that wound healing was fastest in FS and cell proliferation was higher in MS. These results suggest the importance of an optimal serum selection in the experiments involving cell culture system, and gender-specific Hanwoo sera may be used as a substitute to FBS.

Effect of Immunoglobulin Y on Growth Performance and Blood Immunological Parameters in Holstein Calves (난황항체(IgY) 급여가 Holstein 송아지의 성장능력 및 면역관련 혈액 지표에 미치는 영향)

  • Jung, Hee-Sung;Jung, Keun-Ki;Jang, In-Surk
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.321-328
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    • 2009
  • A total of fourteen, 1-wk-old male Holstein calves were allotted into two groups consisted of control (CON) and IGY which was orally administrated with immunoglobulin yolk (IgY) for 1wk. Calves in both groups were provided with milk replacer according to feeding program and had ad libitum access to timothy hay for the entire experimental period (7wks). At 0, 7 and 49 day of experiment, blood samples were collected from the jugular vein of calves to investigate blood biochemical profiles and the differential count (%) of white blood cell (WBC). We also monitored growth performance and colony forming unit (CFU) of fecal microbial population in calves. The adminstration of IgY in calves did not affect body weight and weight gain during 49 days feeding trial compared with control group. The CFU of E. coli and Lactobacilli in the feces of calves were not significantly affected by IgY treatment, whereas the score of the calf scours during day 43 to 49 in IgY group showed a significant (P<0.05) solid type. There were no differences in plasma biochemical components including total protein, albumin, immunoglobulin and the other indicators. As for WBC differential count (%), there was no statistical difference in the percentages of neutrophil, lymphocyte, monocyte, eosinophil and basophil at 0, 7 and 49 days after the oral supplementation of IgY. In conclusion, the oral supplementation of IgY as an immunostimulant did not affect growth performance, fecal microbial population, blood biochemical profile and WBC differential count in Holstein calves.

Effects of Carbon Precursors and Hormones on the Lipogenesis and Lipolysis of Hanwoo Cattle Adipose Tissues

  • Lee, S.C.;Lee, H.J.;Kim, D.W.;Park, J.G.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.3
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    • pp.300-306
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    • 2000
  • This experiment was carried out to determine the contributions of acetate, glucose, amino acids and amino acid metabolites as carbon precursors for the incorporation of radioisotope, in intramuscular and subcutaneous adipose tissue and the effects of insulin on lipogenesis and adrenergic agent, norepinephrine on lipolysis in both tissues. The rate of incorporation of $C^{14}$ labelled acetate, glucose, leucine, isoleucine and ${\alpha}$-ketoisocaproic acid into adipose tissue has been measured in subcutaneous and intramuscular adipose tissues. The rate of incorporation was greater (p<0.05) from acetate than glucose in both subcutaneous and intramuscular adipose tissue and the rate of incorporation of carbon precursors into adipose tissues was greater in subcutaneous than in intramuscular adipose tissues. In comparison of amino acids, the rate was highest (p<0.05) with leucine followed by isoleucine and ${\alpha}$-ketoisocaproic acid in subcutaneous adipose tissue, in which there were no differences. Also, in intramuscular tissue, leucine was highest (p<0.05), and the rate of incorporation decreased in the same order. The rates of carbon precursor incorporation appeared to be higher in subcutaneous than in intramuscular tissue. For incorporation of radio-labelled acetate and glucose into intramuscular adipose tissue. preincubated for 48 hrs with insulin and IGF-1, insulin was the most effective to stimulate the incorporation of precursors in both substrates but there was no difference between insulin and IGF-1 in glucose incorporation. For glyceride-fatty acid synthesis, acetate was significantly (p<0.05) greater than glucose in both subcutaneous and intramuscular adipose tissue, and glyceride-glycerol synthesis was greater (p<0.05) for glucose than acetate in both adipose tissues. The rates of lipogenesis from both precursors were slightly greater in subcutaneous than intramuscular adipose tissue. There was significant (p<0.05) site effect in insulin treatment for glyceride-fatty acid synthesis. But there were no significance in control and norepinephrine. For glyceride-glycerol synthesis, there was no site effect caused by hormonal treatment. Insulin was the most effective (p<0.05) in glyceride fatty acid synthesis, while norepinephrine was the same as control. Compared with control, glyceride-glycerol synthesis from acetate in insulin treatment was significantly (p<0.05) low in subcutaneous, but high in intramuscular tissue. At the same time, in both tissues, it was lower in norepinephrine treatment than in control. Glyceride-glycerol synthesis from glucose was highest (p<0.05) in norepinephrine treatment followed by insulin although there was no significance between insulin and control. Lipolysis was not affected by insulin but was increased by norepinephrine when added to adipose tissue incubations in vitro. Rates of basal lipolysis were greater in subcutaneous adipose tissue than in intramuscular adipose tissue.

Effects of Se and Vit. E Administration on Reproductive Function of Dams and Developmental Ability of Their Calves (Se과 Vit. E 투여가 한우 종빈우의 번식기능과 송아지의 발육성적에 미치는 효과)

  • 황환섭;최재관;박동헌;김종복;박춘근;정희태;김정익;양부근
    • Korean Journal of Animal Reproduction
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    • v.27 no.2
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    • pp.135-142
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    • 2003
  • The objective of this study were to investigate the effects of Se and Vit. E administration at last month or two month before parturition on the reproductive function of dams and developmental ability of calves. On developmental ability of calves obtained afier Se and Vit. E administration at the last month of pregnancing periods, the birth weight were significantly higher in administration groups than in control group(P<0.05). However, the weaning weight, average daily gain and weaning age were not significantly difference in all experimental groups. In the comparision of reproductive function in dams, estrus postpartum was faster in treatment groups than control groups(P>0.05). However, the number of A.1 service fur conception were lower groups injected than control group(P>0.05), there were not significantly differences in all experimental groups. The developmental ability of calves obtained after Se and Vit. E administration at two months before parturition were also examined. The birth weight, weaning weight, average daily gain and weaning age were higher in treatment groups than control group, but there were not significantly differences in all experimental groups. In reproductive function of dams, the days to 1 st estrus postpartum was slightly faster injected groups than control group(P>0.05). Number of A.I. service for conception in each groups were lower in treatment groups than control group.

AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene Expression in Single- and Co-cultured Bovine Satellite Cells and Intramuscular Preadipocytes Treated with Palmitic, Stearic, Oleic, and Linoleic Acid

  • Choi, S.H.;Park, S.K.;Johnson, B.J.;Chung, K.Y.;Choi, C.W.;Kim, K. H.;Kim, W.Y.;Smith, S.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.3
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    • pp.411-419
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    • 2015
  • We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained $10{\mu}g/mL$ insulin and $10{\mu}g/mL$ pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with $40{\mu}M$ palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta ($CPT1{\beta}$) gene expression, but the increase in $CPT1{\beta}$ gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17-fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased $AMPK{\alpha}$ gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism.

Assessment of cryopreserved sperm functions of Korean native brindled cattle (Chikso) from different region research centers of Korea

  • Ma, Lei;Jung, Dae-Jin;Jung, Eun-Ju;Lee, Woo-Jin;Hwang, Ju-Mi;Bae, Jeong-Won;Kim, Dae-Hyun;Yi, Jun Koo;Lee, Sang Moo;Ha, Jae Jung;Kwon, Woo-Sung
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.2
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    • pp.106-115
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    • 2021
  • Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

Effects of spent mushroom (Flammulina velutipes) substrates on in vitro ruminal fermentation characteristics and digestibility of whole crop sorghum silage (팽이버섯 수확후배지 첨가가 수수 사일리지의 in vitro 반추위 발효특성 및 소화율에 미치는 영향)

  • Moon, Yea Hwang;Chang, Sun Sik;Kim, Eun Tae;Cho, Woong Gi;Lee, Shin Ja;Lee, Sung Sil;Cho, Soo Jeong
    • Journal of Mushroom
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    • v.13 no.3
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    • pp.163-169
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    • 2015
  • The in vitro experiment was conducted to ensure the supplemental level of spent Flammulina velutipes mushroom substrates (SMS) as an energy source in manufacturing of whole crop sorghum silage. Sorghum harvested at heading stage was ensiled with spent mushroom substrates of 20% (S-20), 40% (S-40) and 60% (S-60) as fresh matter basis for 6 week. The experiment was conducted by 3, 6, 9, 12, 24, 48 hrs of incubation time with 3 replications. The silages were evaluated fermentation characteristics and dry matter digestibility (DMD) in vitro. The pH of in vitro solution was inclined to decrease with elapsing the incubation time, and that of the S-20 was significantly (P<0.05) lower than the other treatment at 48 hr of incubation. Gas production was greater (P<0.05) in the S-20 than the other treatments at 6 and 12 hrs of incubation. The microbial growth in vitro was inclined to decrease following 24 hr of incubation, and thereafter sustained the similar levels. In vitro dry matter digestibility (IVDMD) was lowered by increasing the supplemental level of spent mushroom substrate, and was a low level in the S-60 throughout whole incubation time. Although the IVDMD for S-40 was steadily increased from 9 hr of incubation and reached to similar level with the S-20 at 48 hour of incubation, however SMS for whole crop sorghum silage fermentation might as well add about 20 to 30% in fresh matter basis when considering DMD.

Changes of Carcass Traits and Surface Meat Color of Korean Cattle (Hanwoo) Reared Different Altitudes or Transferred from Different Distance (고도 및 수송거리별 출하 한우의 도체특성 및 표면육색의 변화)

  • Jang, Yong-Seol;Choi, Chang-Kun;Lee, Jeong-Woo;Kwak, Don-Kyu;Sung, Cheol-Wan;An, Jun-Sang;Park, Byung-Ki;Lee, Jong-In;Shin, Jong-Suh
    • Journal of Animal Science and Technology
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    • v.54 no.1
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    • pp.51-58
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    • 2012
  • The purposes of the study were to examine the changes in carcass traits and surface meat color, and cortisol concentration by different altitudes and shipping distances for steer and cow. The experimental animals were shipped from Kangwondo, Kyunggido, Choongchungdo, and Kyungsangdo to Wonju LPC. The animals were examined for yield traits, quality traits, carcass grade, lightness, redness, yellowness, croma value, and cortisol concentration by different altitudes and shipping distances. The results showed that the carcass traits of steer like back fat thickness were not different by shipping distances of 100 km, 150 km, and 200 km. However, the fat thickness was higher in steers shipped from 250 km than 100 km, 150 km, and 200 km distance. Ribeye area was reduced significantly in 200 km and 250 km than 50 km. Yield index and yield grade were significantly low in 250 km than 50-200 km. However, meat color, fat color, texture, mature, and quality grade had no differences between shipping distances. Marbling score was not different in 50-200 km. However, the marbling score in 250 km was significantly lower than that of 50 km. In case of surface meat color by shipping distance, redness, yellowness, chroma value, and hue-angle were not different in shipping distance of 250 km. The lightness had similar result in 50-200 km. However, in case of 250 km the lightness was significantly low. The REA of cow carcass by shipping distance had no differences by shipping distance. The BET had similar results in 50-150 km. However, it had significantly thick in 200 km. The yield index and yield grade had no differences in 50-150 km. However, yield index and grade were significantly low in 200 km. The carcass trait of cow had no differences in all items by shipping distance. Although the carcass traits and the BET for steer by altitude had no differences between 100, 200, 300 and 500 m, but those were significantly thick in 400 m. And the yield index and the yield grade at altitude 400 m were lower than that of other altitudes. The quality traits and the quality grade had no differences between 100, 200, 300, 400, and 500 m altitudes. The yield traits, quality traits, yield grade, and quality grade had no significant differences by altitudes. In case of yield index of cow for 300 m was low than the cases of 100 m and 200 m. The surface meat color for steer and cow had no differences by altitudes. However, the lightness of cow had positive result in 100 m than 200 m and 300 m. In case of steer and cow the cortisol concentration by shipping distance was high as the shipping distances were longer. However, the cortisol concentrations of steer and cow by altitudes were decreased as the altitudes were increased. From the above results carcass traits and carcass grade were decreased and the cortisol concentration was increased as the altitudes were decreased for steer and cow.

Effects of Saponin Contained Plant Extracts on Ruminal Fermentation Characteristics and Methane Production (Saponin 함유 식물 추출물의 첨가가 반추위 발효성상과 메탄생성에 미치는 영향)

  • Ok, Ji-Un;Baek, Youl-Chang;Kim, Kyoung-Hoon;Lee, Sang-Cheol;Seol, Yong-Joo;Lee, Kang-Yeon;Choi, Chang-Weon;Jeon, Che-Ok;Lee, Sang-Suk;Lee, Sung-Sil;Oh, Young-Kyoon
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.147-154
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    • 2011
  • This study was conducted to evaluate the effects of saponin contained plant extracts on in vitro rumen fermentation characteristics and methane production. Ruminal fluid was collected from rumen cannulated Hanwoo steers fed rice straw and concentrate (5:5). Collected rumen fluids, corn starch and buffer including saponin contained plant extracts (ginseng, Ogapi, soapwort, tea plant and yucca; 0.5%/15 ml) were incubated at $39^{\circ}C$ for 24 h. All incubations were repeated five times. Rumen pH in all treatments was lower (p<0.05) compared with that of the control (no addition) during incubation time. The concentration of total VFA in all treatments was higher (p<0.05) than that of the control after 12h incubation. Compared with the control, the concentration of acetate and propionate in all treatments was lower and higher after 6h incubation, respectively. The concentration of $NH_3$-N in all treatments was lower (p<0.05) than that of the control except for Ogapi or yucca extracts supplementation. The number of protozoa in all treatments was significantly (p<0.05) lower than that of the control except for soapwort extract supplementation. The total gas production and methane production in all treatments was higher (p<0.05) and lower (p<0.05) compared with the control, except for ogapi or soapwort extracts supplementation after 12h incubation, respectively. Therefore, reduction in methane production by saponins may could be results from decreased protozoal population without any negative in vitro fermentation.