• Title, Summary, Keyword: Hanwoo

Search Result 1,509, Processing Time 0.046 seconds

Identification of Differentially Expressed Genes Related to Intramuscular Fat Development in the Early and Late Fattening Stages of Hanwoo Steers

  • Lee, Seung-Hwan;Park, Eung-Woo;Cho, Yong-Min;Kim, Sung-Kon;Lee, Jun-Heon;Jeon, Jin-Tae;Lee, Chang-Soo;Im, Seok-Ki;Oh, Sung-Jong;Thompson, J.M.;Yoon, Du-Hak
    • BMB Reports
    • /
    • v.40 no.5
    • /
    • pp.757-764
    • /
    • 2007
  • Marbling of cattle meat is dependent on the coordinated expression of multiple genes. Cattle dramatically increase their intramuscular fat content in the longissimus dorsi muscle between 12 and 27 months of age. We used the annealing control primer (ACP)-differential display RT-PCR method to identify differentially expressed genes (DEGs) that may participate in the development of intramuscular fat between early (12 months old) and late fattening stages (27 months old). Using 20 arbitrary ACP primers, we identified and sequenced 14 DEGs. BLAST searches revealed that expression of the MDH, PI4-K, ferritin, ICER, NID-2, WDNMI, telethonin, filamin, and desmin (DES) genes increased while that of GAPD, COP VII, ACTA1, CamK II, and nebulin decreased during the late fattening stage. The results of functional categorization using the Gene Ontology database for 14 known genes indicated that MDH, GAPD, and COP VII are involved in metabolic pathways such as glycolysis and the TCA cycle, whereas telethonin, filamin, nebulin, desmin, and ACTA1 contribute to the muscle contractile apparatus, and PI4-K, CamK II, and ICER have roles in signal transduction pathways regulated by growth factor or hormones. The final three genes, NID-2, WDNMI, and ferritin, are involved in iron transport and extracellular protein inhibition. The expression patterns were confirmed for seven genes (MDH, PI4-K, ferritin, ICER, nebulin, WDNMI, and telethonin) using real-time PCR. We found that the novel transcription repressor ICER gene was highly expressed in the late fattening stage and during bovine preadipocyte differentiation. This information may be helpful in selecting candidate genes that participate in intramuscular fat development in cattle.

Genetic Relationship Between Weaning Weight and Carcass Traits in Hanwoo (한우의 이유시 체중과 도체형질과의 유전적 관계)

  • Hwang, J.M.;Choi, J.G.;Kim, H.C.;Choy, Y.H.;Lee, C.;Yang, B.K.;Shin, J.S.;Kim, Jong-Bok
    • Journal of Animal Science and Technology
    • /
    • v.49 no.2
    • /
    • pp.177-182
    • /
    • 2007
  • The objective of this study was to evaluate the genetic relationship between weaning weight (WW) and carcass traits. Carcass traits were eye muscle area(EMA), back fat thickness(BFT), marbling score 1(MS1) in 21 grade scales, marbling score 2(MS2) in 7 grade scales and meat color scores(Mcolor). Parameters were estimated by REML procedure with MTDFREML package. Models included contemporary group as defined by the same year-season-sex at birth, linear covariates of age(days) at weaning, age of dam(days) and age at slaughter(days) as fixed effects and animal random effects for all the traits. Heritability estimates of WW, EMA, BFT, MS1, MS2 and Mcolor were 0.25, 0.20, 0.20, 0.32, 0.32 and 0.22, respectively. Genetic(phenotypic) correlation coefficients of WW with EMA, BFT, MS1, MS2 and Mcolor were 0.75(0.16), 0.18(0.05), -0.41(-0.09), -0.40(0.11) and -0.07(0.05), respectively. Results from this study suggest that single trait selection for weaning weight would lead to progeny population having carcass with large EMA, thick BFT and decreased MS.

Sex Identification of Bovine Meat Using Male Specific SRY and ZFY Genes (웅성 특이적 SRY 및 ZFY 유전자를 이용한 쇠고기 성(性) 판별)

  • Shin, Sung-Chul;Chung, Ku-Young;Chung, Eui-Ryong
    • Food Science of Animal Resources
    • /
    • v.27 no.3
    • /
    • pp.351-356
    • /
    • 2007
  • The objective of this study was to develop a rapid and reliable method for the sex determination of beef using the PCR(polymerase chain reaction) technique. We have used two bovine sex determining genes, SRY and ZFY, on the Y-chromosome to identify the sex of Hanwoo and Holstein beet. We attempted to amplify 1,348 bp and 979 bp fragments from male and female genomic DNA corresponding to the SRY and ZFY genes, respectively, using male specific primers. The amplified PCR products were separated by electrophoresis in a 1.5% agarose gel to detect a male specific DNA band. When DNA from male beef was amplified with primers specific for the SRY gene, a DNA band of 1,348 bp was present in all of the male samples, but absent from all of the female samples. Also, when DNA from male beef was amplified with primers specific for the ZFY gene, a DNA band of 979 bp was observed in all of the male samples, but absent from all female samples. In conclusion, the bovine SRY and ZFY genes are typically found only in male beef. For the practical application of this method for the sexing of commercial beef at the processing and marketing stages after slaughter. a total of 350 beef samples collected randomly from local markets were analyzed for sex determination. The proportions of male and female samples were 252 (72%) and 98 (28%), respectively. Therefore. the SRY and ZFY genes. which are specific for the Y-chromosome, may be useful sex-diagnostic DNA markers to distinguish male meat from female meat.

Discrimination of Irradiated Beef Using Comet Assay (Comet assay를 이용한 방사선 조사육의 판별)

  • Jeong, Seok-Kyu;Park, Jong-Heum;Ji, Seung-Taek;Park, Kum-Ju;Kim, Hai-Hong;Hyun, Chang-Kee;Shin, Heuyn-Kil
    • Korean Journal of Food Science and Technology
    • /
    • v.32 no.4
    • /
    • pp.747-754
    • /
    • 2000
  • DNA damages in post-mortem bovine muscle samples caused by gamma irradiation at doses of 1 to 10 kGy were determined by Comet assay. When the cell extract was prepared in a physical method and followed by neutral lysis and neutral electrophoresis, the optimal comet images could be obtained. DNA damages were evaluated from the mean tail length, the distributions of comet images in 4 groups divided by tail length and the relative damage index (RDI) values calculated from the distribution pattern. The mean tail length and RDI value were increased by increasing the irradiation dose, and the RDI value was found to be useful as an index for discriminating of irradiation and measuring the irradiated dose. Blind tests using Korean domestic (Hanwoo) and imported beef samples showed a higher RDI value for the latter. However, the value was lower than those of irradiated samples indicating that the cause of DNA damages in the imported beef samples might be not irradiation but low-temperature treatments. It was concluded from the results of this study that the irradiated beef and its irradiated dose could be detected and predicted by Comet assay.

  • PDF

Effects of In Vitro Maturation, In Vitro Fertilization and In Vitro Culture Conditions on Bovine Embryo Production (체외성숙, 수정 및 배양에 있어서 각기 다른 배양조건들이 소 체외수정란의 생산에 미치는 효과)

  • 조성근;송상현;공일근;이효종;최상용;박충생
    • Journal of Embryo Transfer
    • /
    • v.15 no.1
    • /
    • pp.67-75
    • /
    • 2000
  • This study was conducted to establish the optimal conditions for in vitro embryo production using oocytes derived from follicles of slaughter-house ovaries. The ovaries of Hanwoo were obtained from a local slaughter-house. The oocytes were aspirated from visible follicles of 2~7mm in diameter. The recovered oocytes which were completely surrounded by at least 2 layers of cumulus cells and a homogeneous cytoplasmic pigmentation were used. The selected oocytes were washed 3 or 4 times with D-PBS containing 10% bovine calf serum (BCS) and matured in vitor (IVM) in Ham's F-10 supplemented with 10% BCS or 0.01 $\mu\textrm{g}$/ml epidermal growth factor(EGF) at 39$^{\circ}C$ under 5% CO2 in air for 24 hours. They were fertilizqed in vitro (IVF) with fresh sperm separated by Percoll density gradient or swim-up in TALP media. The zygotes were cultrued with or without bovine oviductal epitherial cells(BOEC) in media(HECM-6 supplemented with 11 amino acid and / or TCM-199 supplemented with 10% BCS) for 7 to 10 days. The results obtained were as follow: The cleavage rate and developmental rate to blastocyst after maturation and IVF were not significantly different between Ham's F-10 with EGF(76.0% vs. 44.0%) and BCS(75.9% vs. 43.6%)(P<0.05). The cleavage rate and development rate to blastocyst after fertilizing by swim-up or Percoll method were not signifciantly(P<0.05) different between swim-up (80.2% vs. 29.2%) and Percoll(81.9% vs. 26.5%) (P<0.05). The cleavage rate in TCM 199(80.5) was signficiantly higher than that in HECM-6 (72.0%) (P<0.05). However, developmental rate to blastocyst using TCM 199 following HECM-6 for 3 or 4 days (42.2%) was significantly higher than that in TCM-199 alone(26.7%)(P<0.05). The cleavage rate and development rate of embryos produced in vitro by exchange timing for HECM-6 media were not significantly different between in day 3(78.6% vs. 45.5%) and day 4(75.0% vs. 43.2%)(P<0.05). The cleavage rate and developmental rate to blastocysts according to co-culture system were not significantly different between with (74.2% vs. 41.4%) and without BOEC(73.95 vs. 43.5%) (P<0.05). The number of blastomere in blastocyst stage after co-culture with or without BOEC was not significantly different (106.7$\pm$5.1 and 96.6$\pm$4.0). In conclusion, the most transferable IVP embryos could be produced from Ham's F-10 medium for IVM, Percoll density gradient method for IVF sperm separation and in vitro culture in HECM-6 until day 3 or day 4, and then transferred into TCM-199 until day 9 within adequate embryo density in culture droplets after insemination.

  • PDF

Administration of encapsulated L-tryptophan improves duodenal starch digestion and increases gastrointestinal hormones secretions in beef cattle

  • Lee, Sang-Bum;Lee, Kyung-Won;Wang, Tao;Lee, Jae-Sung;Jung, U-Suk;Nejad, Jalil Ghassemi;Oh, Young-Kyoon;Baek, Youl-Chang;Kim, Kyoung Hoon;Lee, Hong-Gu
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.33 no.1
    • /
    • pp.91-99
    • /
    • 2020
  • Objective: This study investigated the effects of oral administration of rumen-protected L-tryptophan (RPL-T) on duodenal starch digestion and gastrointestinal hormones (GIH) secretions using Hanwoo beef steers as the animal models. Methods: Four steers (423±24 kg) fitted with ruminal and duodenal cannulas were employed in a crossover design replicated twice. Treatments were control (basal diet) and RPL-T (basal diet+191.1 mg/kg body weight [BW]) group. Blood and duodenal samples were collected to measure serum GIH levels and pancreatic α-amylase activity at day 0, 1, 3, and 5 (-30, 30, 90, 150, and 210 min) of the study. Samples from each segment of the gastrointestinal tract were collected via ruminal and duodenal cannulas and were used to determine soluble protein and the starch digestion rate at days 6 (-30, 180, 360, and 540 min) and 8 (-30, 90, 270, and 450 min) of the experiment. Results: No significant difference in ruminal pH, NH3-N, and total volatile fatty acid including the levels of acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, and the acetate-to-propionate ratio was observed between groups (p>0.05). Crude protein uptake was higher and feces starch content was lower in RPL-T group than the control group (p<0.05). The D-glucose contents of feces in RPL-T group decreased at day 5 compared to those in the control group (p<0.05), however, no change was found at day 0, 1, or 3 compared to the control group (p>0.05). Serum cholecystokinin (CCK), melatonin, duodenal pancreatic α-amylase activity, and starch digestion were significantly higher in RPL-T group than the control group (p<0.05). Conclusion: Taken together, oral administration of RPL-T at the rate of 191.1 mg/kg BW consistently increased CCK concentration, pancreatic α-amylase activity in duodenal fluids, and starch digestion rate in the small intestine and thus found to be beneficial.

Glutathione (GSH) Concentration and Developmental Competence of Korean Native Cow Oocytes Selected by Brilliant Cresyl Blue (BCB)

  • Lee, Hyo-Sang;Yu, Dae-Jung;Kwon, Tae-Hyeon;Cho, Su-Jin;Bang, Jae-Il;Park, Sang-Guk;Cho, Sung-Kyun;Kong, Il-Keun
    • Journal of Embryo Transfer
    • /
    • v.24 no.1
    • /
    • pp.39-45
    • /
    • 2009
  • This study was carried out to evaluate the nuclear, cytoplasmic maturation and developmental potential of bovine oocytes selected by brilliant cresyl blue (BCB) as indirect measurement of oocytes growth phase. Cumulus-oocyte complexes (COCs) were collected from 2 to 8 mm follicles from slaughterhouse Hanwoo ovaries. The COCs were divided into stained cytoplasm to blue (BCB+) and unstained (BCB-) according to their ooplasm BCB coloration stained by $26{\mu}m$ of BCB after 90 min. Selected COCs were cultured in a TCM 199 for 18 to 26 h. Nuclear maturation and total cell number was evaluated after in vitro maturation (IVM) or in vitro culture (IVC) using $10{\mu}g/ml$ Hoechst 33342, and cytoplasmic maturation was evaluated by intracellular glutathione (GSH) assay before (0 h) and after (24 h) IVM. The oocyte diameters were not differed significantly between BCB+ ($157.4{\pm}5.8{\mu}m$) and BCB+ ($149.0{\pm}31.0{\mu}m$) groups (p>0.05). However, the proportion of metaphase II oocytes in BCB+ group was significantly higher than BCB- group after IVM (p<0.05). GSH content of BCB+ group oocytes was significantly higher than that of BCB- group just after collection ($7.3{\pm}0.6$ vs. $4.8{\pm}0.6\;pmol/oocyte$, p<0.05), but not varied after IVM($13.1{\pm}0.9$ and $12.6{\pm}2.5\;pmol/oocytes$ for BCB+ and BCB- respectively; p>0.05). The proportion of blastocyst formation and total cell number in BCB+ group (23.5% and $105.5{\pm}28.6$) was significantly higher than that in BCB- (9.8% and $72.4{\pm}26.1$; p<0.05). The results indicate that BCB+ group oocytes may provide a cellular and functional basis for the greater developmental competence in Korean Native Cow (KNC) oocytes.

A study on the early pregnancy diagnosis by changing of plasma progesterone concentration and morphology of ovary in pregnancy and non -pregnancy cows (소에서 비임신 및 임신 상태의 난소 형태와 혈중 progesterone 농도 변화에 의한 조기 임신진단)

  • Kim, Cheol-Ho;Bhak, Jong-Sik;Shin, Jung-Sub;Kang, Chung-Bo
    • Korean Journal of Veterinary Service
    • /
    • v.31 no.3
    • /
    • pp.397-414
    • /
    • 2008
  • In order to evaluate conception rate of Hanwoo in northwestern region of Gyeongsang-nam-do, we investigated conception rate and reduction of reproductive disorder rate after artificial insemination (AI) in 1,000 heads of breeding cows, This study showed that 80.9% of cows were classified as fertility after 1st and 2nd AI. For a accurate pregnancy diagnosis with practicing ovariectomy and histeotomy, we comparatively investigated each of 80 slaughtered cows, including 30 of non-pregnancy, and used enzyme-linked immunosorbent assay (ELISA) for estimation of plasma progesterone concentration and serum luteal hormone. The mean diameter of non-pregnant corpus luteum is $18.9{\pm}4.2{\times}15.6{\pm}3.6 mm$ and that of pregnant corpus luteum is $22.5{\pm}2.7{\times}18.7{\pm}2.9 mm$. This indicates that corpus luteum is more developed in the ovary of pregnant than non-pregnant cows (P<0.05). The diameter of pregnant corpus luteum according to the stage of pregnancy showed $21.3{\pm}2.4{\pm}18.4{\pm}2.6 mm$ in early stage (1-3 month), $23.4{\pm}2.8{\times}19.1{\pm}2.7 mm$ in middle stage (4-6 month) and $22.8{\pm}3.0{\times}18.8{\pm}2.4mm$, in last stage (7-9 month). This indicates that corpus luteum in middle and last stage is more significantly developed than that of early stage(P<0.05). The mean plasma progesterone concentration of cows showing size of non-pregnant corpus luteum was $4.58{\pm}0.92ng/ml$ and that of pregnant corpus luteum $8.26{\pm}0.98ng/ml$. Thus, it was more significantly increased in pregnant corpus luteum(P<0.02).. However, it was low to $0.58{\pm}0.39ng/ml$. in estrus (corpus albicans). The plasma progesterone concentration according to gestation period was high in proportion to the degree of development in corpus luteum and more significantly increased (P<0.05) and maintained in middle and last state than early state. The concentration was sharply decreased to $0.56{\pm}0.32ng/ml$ at parturition. As a consequence, we can practice the early pregnancy diagnosis by confirming non-pregnancy when the mean plasma progesterone concentration is below 1ng/ml 19 to 22 days after AI and this can be available to diagnose reproductive disorder.

Effects of Body Condition Score and Estimation of Growth Curves for Chest Girth and Ultra Sonic Longissimus Muscle Area, Backfat Thickness and Marbling Scores in Hanwoo(Korean cattle) Cows (한우 암소의 흉위, 초음파 측정 배장근단면적, 등지방두께, 근내지방도에 대한 발육곡선 추정 및 신체충실지수 효과)

  • Lee, Deuk-Hwan;Lee, Gil-Hwan;Cho, Chung-Il;Kim, Nae-Soo
    • Journal of Animal Science and Technology
    • /
    • v.50 no.5
    • /
    • pp.581-590
    • /
    • 2008
  • Growth curves for ultrasonic carcass traits such as longissimus muscle area, backfat thickness and marbling score as well as chest girth which was simultaneously measured when carcass traits were investigated using ultrasound measuring technique were estimated to identify growth patterns and to adjust maturing effects in order to evaluating genetic merits on cows in farming basis. 27,410 records from 22,451 cows on which of 15~90 month of age were investigated from the national wide of Korea using by ultrasonic scanning techniques by the skilled persons from 2002 to 2007. Van Bertalanffy growth function was applied for estimating growth curves on these traits. Carcass traits and chest girth would be linearly increased by body condition score. It might be used for multiplicative correction factors for pre- adjustment on the body condition scores. Growth pattern on chest girth would be quickly reached to mature size and stable on after reached to asymptotic mature size. Longissimus muscle area would also be reached to mature size but little smoother than chest girth. Otherwise, growth curve on backfat thickness would be steadily increasing up to 7 years of age. It also showed large individual difference by way of mean square error. Marbling score would be steadily increased but sharper than those on backfat thickness. It would be reached to mature size up at 5 years of age. Those growth curves would be used for correcting function on age at investigating on genetic evaluation system.

Effects of Collection Time on Physical Characteristic and Nutritive Value of Rice Straw (볏짚 수거시기가 물리적 특성 및 사료가치에 미치는 영향)

  • Kim, Ung-Duk;Lee, Sang-Moo;Hwang, Joo-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.29 no.3
    • /
    • pp.217-226
    • /
    • 2009
  • This study was carried out to investigate the effects of collection time on the physical characteristics, Nutritive value, digestibility and palatability in rice straw. Collection time of rice straw after the rice harvest was allocated into nine treatments: T1 (10 days), T2 (25 days), T3 (40 days), T4 (55 days), T5 (70 days), T6 (85 days), T7 (100 days), T8 (115 days) and T9 (130 days). The results are summarized as follow: Plant length, leaf length and green degree were decreased with prolonging collection time. Mold distribution and dry matter loss were increased with prolonging collection time. Crude protein and crude fat were decreased with prolonging collection time, crude fiber were increased with prolonging collection time. But crude ash showed multifarious. In situ dry matter digestibility was decreased with prolonging collection time. Palatability of Hanwoo, Holstein and deer was decreased with prolonging collection time. The results of this study indicate that good rice straw collection time would be recommendable during the from 10 days to 40 days after rice straw harvest.