• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.20-20
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.97-97
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• 2017

Plant genomes include heterochromatic loci that consist of repetitive sequences and transposable elements. LTR retrotransposon is the major class of transposons in advanced plants in terms of proportion in plant genome. The elements contribute not only to genome size but also to genome stability and gene expression. A number of cases have been reported transposon insertions near genic regions affect crop traits such as fruit pigments, stress tolerance, and yields. Functional LTR retrotransposons produce extrachromosomal DNA from genomic RNA by reverse transcription that takes place within virus-like-particles (VLPs). DECREASED DNA METHYLATION 1 (DDM1) plays important roles in maintaining DNA methylation of heterochromatin affecting all sequence contexts, CG, CHG, and CHH. Previous studies showed that ddm1 mutant exhibits massive transcription of retrotransposons in Arabidopsis, but only few of them were able to create new insertions into the genome. RNA-dependent RNA POLYMERASE 6 (RDR6) is known to function in restricting accumulation of transposon RNA by processing the transcripts into 21-22 nt epigenetically activated small interfering RNA (easiRNA). We purified VLPs and sequence cDNA to identify functional LTR retrotransposons in Arabidopsis ddm1 and ddm1rdr6 plants. Over 20 LTR copia and gypsy families were detected in ddm1 and ddm1rdr6 sequencing libraries and most of them were not reported for mobility. In ddm1rdr6, short fragments of ATHILA gypsy elements were detected. It suggests easiRNAs might regulate reverse transcription steps. The highest enriched element among transposon loci was previously characterized EVADE element. It has been reported that active EVADE element is more efficiently silenced through female germline than male germline. By genetic analyses, we found ddm1 and rdr6 mutation affect maternal silencing of active EVADE elements. DDM1-GFP protein accumulated in megaspore mother cell but was not found in mature egg cell. The fusion protein was also found in early embryo and maternal DDM1-GFP allele was more dominantly expressed in the embryo. We observed localization of DDM1-GFP in Arabidopsis and DDM1-YFP in maize and found the proteins accumulated in dividing zone of root tips. Currently we are looking at cell cycle dependency of DDM1 expression using maize system. Among 10 AGO proteins in Arabidopsis, AGO9 is specifically expressed in egg cell and shoot meristematic cells. In addition, mutation of AGO9 and RDR6 caused failure in maternal silencing, implying 21-22 nt easiRNA pathway is important for retrotransposon silencing in female gametophyte or/and early embryo. On the other hand, canonical 24 nt sRNA-directed DNA methylation (RdDM) pathways did not contribute to maternal silencing as confirmed by this study. Heat-activated LTR retrotransposon, ONSEN, was not silenced by DDM1 but the silencing mechanisms require RdDM pathways in somatic cells. We will propose distinct mechanisms of LTR retrotransposons in germline and somatic stages.

• 한국구조물진단유지관리공학회 논문집
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• 제22권5호
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• pp.82-90
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• 2018

원자력 발전소에는 No.36(D36)이상의 대구경 철근이 사용되는데 이러한 대구경 철근으로 갈고리 정착을 할 경우, 기준에서 요구하는 구부림 및 갈고리 길이로 인해 설계 및 배근에 있어 큰 어려움을 겪을 수 있다. 이러한 문제를 해결하기 위한 방안으로 확대머리 철근을 사용할 수 있다. 2008년 개정된 ACI 318에서는 확대머리철근의 정착길이식을 도입하였으며, 제정 배경 연구를 근거로 하여 횡보강근의 영향력을 무시하고 있다. 그러나 확대머리 철근이 겹침이음이나 컷오프 구간에서 사용될 경우, 인장재에 의해 피복 콘크리트를 밀어내는 힘이 발생하여 횡보강근에 작용하는 인장력이 크게 증가한다. 본 연구의 목적은 휨을 받는 부재 내에 정착된 확대머리 철근의 정착성능에 대한 횡보강근의 영향력을 평가하는 것으로, 이를 위해 횡보강근의 간격을 변수로 한 대구경 확대머리 철근의 정착실험을 수행하였다. 실험방법으로는 컷오프 구간을 모사한 실험을 수행하였으며, 확대머리 철근으로는 D43의 대구경 철근을 사용하였다. 실험 결과, 횡보강근이 없는 실험체의 경우 정착구간의 쪼갬파괴에 이어 단부의 하중이 확대머리 부근의 콘크리트에 직접적으로 작용하면서 상부 피복 콘크리트가 부재에서 탈락하는 취성적인 파괴형태가 나타났다. 또한 확대머리 철근의 발현강도가 항복강도의 절반밖에 못 미치는 매우 낮은 내력을 보였다. 이에 반해 횡보강근이 배근된 실험체의 경우 경우 횡보강근이 실험체 단부의 하중에 직접적으로 저항함에 따라 실험체 내력이 큰 폭으로 상승하였다.

• 지역사회간호학회지
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• 제12권1호
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• pp.226-238
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• 2001

This study was attempted to understand experiences of the users of natural family planning(NFP) by applying Q-methodology. 37 statements were used to Q population through literature research and interviews. For the P sample. I interviewed personally 30 people who had taken a lesson in NFP for over 6 months and had been using and experiencing it autonomously for more than 2 years so far, and asked them to Q-classify the statement cards in order of the degree of approval, from the one they most approved to the one they least approved. Data were coded and input into a computer and were analyzed using QUANL PC Program. In this study, I discovered 4 types of experiences by the users of NFP and named them each according to their features: the first, a type of mutual control with morality, the second, a type of the pursuit of mutual -cooperation, the third. a type of the pursuit of conviction. and the fourth, a type of the perception of health management. The first type said that NFP was a good contraceptive way in which no drug or devices are used, that the husband's cooperation was needed, and that they became able to control sexual desire with morality. The second type perceived that continuous efforts were required to put NFP in practice, that the, husband's cooperation was essential, and that dialogues between husband and wife were also necessary to use it, and as such they regarded mutual cooperation between husband and wife as highly important. On the other hand. the third type perceived that one's conviction played a big part, that knowledge of physiological changes was useful and the ability to sense physical changes improved. and that one's willpower was important. and thus they regarded one's own willpower as most important. The fourth type reported that this method was a good way to control pregnancy and should be taught about at the beginning of sex education. and thus they were emphasizing the importance of NFP as a helpful way to manage one's health. 1. therefore, intend to provide the following suggestions based on the result of this study. 1) It is necessary to develop a program that allows NFP to be applied to sex education program. 2) It is necessary to analyze factors that affect the execution of NFP. 3) It is necessary to analyze causes of failure of those who fail to execute NFP.

• Advances in aircraft and spacecraft science
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• 제11권1호
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• pp.83-103
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• 2024

Adhesive bonding is currently widely used in many industrial fields, particularly in the aeronautics sector. Despite its advantages over mechanical joints such as riveting and welding, adhesive bonding is mostly used for secondary structures due to its low peel strength; especially if it is simultaneously exposed to temperature and humidity; and often presence of bonding defects. In fact, during joint preparation, several types of defects can be introduced into the adhesive layer such as air bubbles, cavities, or cracks, which induce stress concentrations potentially leading to premature failure. Indeed, the presence of defects in the adhesive joint has a significant effect on adhesive stresses, which emphasizes the need for a good surface treatment. The research in this field is aimed at minimizing the stresses in the adhesive joint at its free edges by geometric modifications of the ovelapping part and/or by changing the nature of the substrates. In this study, the finite element method is used to describe the mechanical behavior of bonded joints. Thus, a three-dimensional model is made to analyze the effect of defects in the adhesive joint at areas of high stress concentrations. The analysis consists of estimating the different stresses in an adhesive joint between two 2024-T3 aluminum plates. Two types of single lap joints(SLJ) were analyzed: a standard SLJ and another modified by removing 0.2 mm of material from the thickness of one plate along the overlap length, taking into account several factors such as the applied load, shape, size and position of the defect. The obtained results clearly show that the presence of a bonding defect significantly affects stresses in the adhesive joint, which become important if the joint is subjected to a higher applied load. On the other hand, the geometric modification made to the plate considerably reduces the various stresses in the adhesive joint even in the presence of a bonding defect.

• 한국터널지하공간학회 논문집
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• 제24권6호
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• pp.451-464
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• 2022

고준위방사성폐기물 처분장은 심지층 처분시스템으로 사용후핵연료를 취급하는 특성상 고온, 방사선 및 지하수 등의 복합적인 환경조건에 노출되어 있다. 지속적인 노출에 의해 시간이 지남에 따라 구조물의 균열 및 열화가 발생할 수 있다. 한편 고준위방사성폐기물 처분장은 초장기 기대수명이 요구되며 이에 따른 장기적인 구조물 건전성 모니터링이 필수적이다. 구조물 건전성 모니터링에는 가속도계, 토압계, 변위계 등 다양한 센서들이 활용될 수 있으며, 이 중 일반적으로 피에조센서가 사용된다. 따라서 피에조센서의 내구성 평가를 바탕으로 고내구성 센서를 개발할 필요가 있다. 본 연구에서는 피에조센서의 내구성 평가 및 수명예측을 위한 가속수명시험을 설계하였다. 문헌연구를 바탕으로 단일 스트레스 인자에 대한 가속 스트레스 수준 수 및 각 수준 별 시료 수를 선정하였다. 또한 고준위방사성폐기물 처분장 환경조건에서 발생할 수 있는 피에조센서의 고장모드 및 고장메커니즘을 분석하였다. 온도 스트레스 인자에 대한 최대 가혹조건 탐색 실험을 두 가지 방법으로 제안하였으며 피에조센서의 신뢰도 높은 동작한계를 도출하였다. 이를 이용하여 가속수명시험의 합리적인 가속 스트레스 수준을 설정하였다. 본 연구에서 제시된 최대 가혹조건 탐색 실험방법은 경제적이며 실용적인 아이디어를 담고 있으며, 추후 피에조센서의 가속수명시험 설계에 널리 활용될 수 있을 것으로 판단된다.

• KDI Journal of Economic Policy
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• 제12권3호
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• pp.71-94
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• 1990

본고(本稿)서는, 최근 자유금융학파(自由金融學派)와 신화폐경제학과(新貨幣經濟學科)들의 등장으로 화폐(貨幣) 금융문제(金融問題)에서의 자유경쟁(自由競爭) 및 자유방임주의적(自由放任主義的) 사고가 새롭게 확산되고 있는 시점(時點)에서, 정부(政府) 및 중앙은행(中央銀行)의 화폐(貨幣) 금융개입(金融介入)의 이론적(理論的) 근거(根據)와 그에 관련된 논쟁(論爭)을 다음의 6가지 논거(論據)들을 중심으로 개관해 보았다 : (1) 자유금융하(自由金融下)의 銀行券(은행권) 초과발행(超過發行) 가능성(可能性), (2) 화폐사용에 있어서의 외부경제효과(外部經濟效果)와 화폐제도의 공공재적(公共財的) 성격(性格) (3) 화폐발행업무의 규모(規模)의 경제(經濟)와 자연독점적(自然獨占的) 성격(性格), (4) 실물부문(實物部門)의 불안정성(不安定性)과 거시안정화정책(巨視安定化政策)의 필요성, (5) 은행금융시장(銀行金融市場)의 불안정성(不安定性)과 은행파산(銀行破産)의 외부효과(外部效果), (6) 소액거래자(少額去來者) 및 예금자(預金者)의 보호(保護) 이러한 논거들에 의하면 외부화폐(外部貨幣)(outside money)의 공급은 전형적인 공공재이론(公共財理論)이나 기술적(技術的) 독점주장(獨占主張)이 적용되는 경우이기 때문에 외부화폐제도(外部貨幣制度)의 유지에 있어서 정부(政附)나 중앙은행(中央銀行) 독점(獨占) 및 개입(介入)이 불가피하고 또한 바람직하지만, 내부화폐(內部貨幣)(inside money)제도(制度)의 경우는 적절한 최소한의 안전장치만 강구된다면 최근의 자유금융학파(自由金融學派) 및 신화폐경제학과(新貨幣經濟學科)들의 주장과 같이 사적(私的) 자유경쟁(自由競爭)이 보다 활성화되도록 하는 것이 바람직할 것으로 판단된다 . 한편 외부화폐제도(外部貨幣制度)에의 개입(介入)에 따른 (정부(政府) 및) 중앙은행(中央銀行)의 거시통화정책기능(巨視通貨政策機能)은 물론, 보다 자유화(自由化)된 내부화폐제도하(內部貨幣制度下)에서도 중앙은행(中央銀行)의 최종대여자기능(最終貸與者機能)과 미시적(微視的) 감독기능(監督機能)은 동(同) 제도(制度)의 안전성(安全性)을 유지하기 위해 중요한 역할을 할 것으로 보인다.

• 한국환경농학회지
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• 제15권4호
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• pp.406-418
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• 1996

침수의 깊이와 시간에 따른 토마토의 생육단계별 생육저해정도와 수량 감소 및 이와 관련된 이병성, 시비효과, 형태적 특성 및 생리적 변화 등의 차이를 비교 검토하여 침수피해에 따른 피해정도의 예측에 기초자료로 활용하기 위하여 온실조건에서 발아기, 유묘기별로 각각 침수깊이를 지면위 0, 5. 10, 15 cm의 4개 처리에, 각각 시간을 6, 12, 24, 48, 120 시간으로 처리하였으며, 그 결과는 다음과 같다. 1. 발아기(發芽期) 만수(漫水) 반응(反應) $0{\sim}5$ cm 침수에서 48시간, $10{\sim}15$ cm 침수에서는 $25{\sim}27$시간을 기점으로 현저한 발아제한이 이루어졌다. 초장은 침수깊이에 관계없이 24시간 이내까지 회복이 가능했고, 엽수는 침수깊이가 증대됨에 따라 회복 가능한 시간의 한계가 짧아졌다. 생체중의 감소는 지하부보다 지상부에서 민감한 반응을 보이고, 침수경과 24이내에서 회복이 가능하였다. 침수처리별 발아율과 초장, 엽수, 지상 및 지하부 생체중간의 모든 형질간에 고도의 유의적 상관 관계가 있었다. 2. 유묘기(幼苗期) 만수(漫水) 반응(反應) 초장은 0 cm 깊이에서는 24시간 처리수준까지 초장회복이 되었고, 그 이상 깊이에서는 시간과 관계없이 유의적인 감소가 되었으며, 엽수에서는 0 cm에서는 120시간까지도 회복되었고, 5 cm에서는 24시간까지 회복이 가능하였으나, 그 이상 깊이에서는 6시간 이상처리에서 유의적인 감소가 있었다. 지상 및 지하부 생장량은 초장 및 엽수에서와 같은 경향을 보였다. 최장근신장이 0 cm 침수에서는 대조구의 80% 정도로 유지될 수 있었지만 5 cm이상 깊이에서는 120시간 침수로 근신장이 불가능 하였다. 근활력은 0 cm이상의 침수로 현저히 감퇴되었고, 호흡량은 5 cm 깊이를 변환점으로 하여 현저한 감소가 야기되었다. 그러나 광합성량은 침수깊이에 따라 직선적으로 감소하였다. 엽록소 함량은 침수깊이를 달리하여 48시간까지는 완만한 감소를 보였으나, 120시간에서는 현저한 엽록소 분해가 초래되었다. 발병은 침수처리 깊이와 시간 증대에 비례하여 커지는 경향이었고, 살균제 처리에 따른 방제의 가능성은 있었으나, 질소엽면시비 효과는 없었다. 개체당 총과중과 총과수는 침수깊이와 시간이 길어 질수록 완만하게 감소하였고, 평균 과중은 증대 되었으며, 침수깊이와 시간간의 통계적인 상호작용 효과는 없었다. 생장 및 수량 특성 모든 조사항목들 상호간에 고도의 유의성 있는 정의 상관관계를 나타내었다.

• 대한치과보철학회지
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• 제41권3호
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• pp.369-378
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• 2003

Statement of Problem: Recently, resin cements have become more widely used and have been accepted as prominent luting cements. Current resin cements exhibit less microleakage than conventional luting cements. However, the constant contact with water and exposure to occlusal forces increase microleakage even in resin cements inevitably. Most bonding resins have been modified to contain a hydrophilic resin such as 2-hydroxyethylmethacrylate (HEMA) to overcome some of the problems associated with the hydrophobic nature of bonding resins. By virtue of these modifications, bonding resins absorb a significant amount of water, and there may also be significant stresses at bonding interfaces, which may adversely affect the longevity of restorations. Therefore the reinforcement of water stability of resin cement is indispensable in future study. Purpose: This study was conducted to examine the influence of water retention on microleakage of two resin cements over the period of 6 months. Materials and Methods: 32 extracted human teeth were used to test the microleakage of a single full veneer crown. Two resin cements with different components and adhesive properties - Panavia F (Kuraray Co., Osaka, Japan) and Super-Bond C&B (Sun Medical Co., Kyoto, Japan)- were investigated. The storage medium was the physiological saline solution changed every week for 1 month, 3 months, and 6 months. One group was tested after storage for 1 day. At the end of the each storage period, all specimens were exposed to thermocycling from $5^{\circ}C$ to $55^{\circ}C$ of 500 cycles and chewing simulation of 50,000 cycles, and then stained with 50% silver nitrate solution. The linear penetration of microleakage was measured using a stereoscopic microscope at ${\times}40$ magnification and a digital traveling micrometer with an accuracy of ${\pm}3{\mu}m$. Values were analyzed using two-way ANOVA test, Duncan's multiple range tests (DMRT). Results : Statistically significant difference of microleakage was shown in the 3-month group compared with the1-day or 1-month group in both systems (p<0.05) and there were statistically significant differences in microleakage between the 3-month group and the 6-month group in both systems (p<0.05). The two systems showed different tendency in the course of increased microleakage during 3 months. In Panavia F, microleakage increased slowly throughout the periods. In Super-Bond C&B, there was no significant increase of microleakage for 1 month, but there was statistically significant increase of microleakage for the next 2 months. For the mean microleakage for each period, in the 3-month group, microleakage of Super-Bond C&B was significantly greater than that of Panavia F. On the other hand, in the 6-month group, microleakage of Panavia F was significantly greater than that of Super-Bond C&B (p<0.05). Conclusion: Within the limitation of this study, water retention of two different bonding systems influence microleakage of resin cements. Further studies with the longer observation periods in viro are required in order to investigate water stability and the bonding durability of the resin cement. CLINICAL IMPLICATIONS Microleakage at the Cement-tooth interfaces did not necessarily result in the failure of the crowns. But it is considered to be a major factor influening the longerity of restorations. Further clinical approaches for decreasing the amount of microleakage are required.

• Clinical and Experimental Reproductive Medicine
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• 제4권1호
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• pp.17-25
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• 1977

Anderson(1937), Power and Barnes(1941) reported a study concerning a method of tubal sterilization in association with peritoneoscopy or laparoscopy in which they cauterized the tubes. There appears to have been a hiatus of interest in sterilization (cold or hot) associated with laparoscopy until reintroduction by Palmer(1963), Frangenheim(1964) and Steptoe(1967). On the other hand, for interval female sterilization, however, minilaparotomy is relatively new. By Saunder and Munsick(1972), John Lyle(1974), Frank Stubb(1974), Vitoon(1973) and B.C. Bai(1975), their own technique for interval female sterilization requires 2.0 to 2.5cm, incision at the margin of the mons pubis. In Korea, female sterilization by means of minilaparotomy firstly reported by B.C. Bai using Bai's uterine elevator, of his own device, early in 1975. Recently inteval female sterilization by laparoscopy and minilaparotomy are widely accepted throughout the world especially in Asian countries. Minilaparotomy is carried out from 1974, laparoscopic sterilization from 1976, and in this study each of 250 cases of those were analysed and discussed for the comparison at Seoul Red Cross Hospital. (1) In the age distribution, numerous clients were in their age of $31{\sim}35$ in laparoscopy as well as minilaparotomy. Average 33.7 years in L and 33.2 years in M. (M=minilaparotomy, L=laparoscopic sterilization) (2) As regarding living children, women having 3 children represented the greatest number, 113 cases out of 250 in M group and 102 cases out of 250 in L group. Average No. of child are 2.9 in Land 3.1 in M. (3) Concidering the operation day in the menstrml cycle, the greatest number of cases, those who underwent tubal sterilization during the days of $26{\sim}$, next during the $6{\sim}10$ days of the cycle in both group. (4) Concidering the operation time, 188 cases by laparoscopy were performed in $6{\sim}10$ minutes, 33 cases within 5 minutes and 24 cases in $11{\sim}15$ minutes. Maximum 50 minutes, minimum 4 minutes and average 8.3 minutes. The majority of cases (154 cases) by minilaparotomy required $6{\sim}10$ minutes and 67 cases $11{\sim}15$ minutes, 6 cases within 5 minutes. Maximum 30 minutes, minimum 4 minutes and average 10.4, minutes. In both groups, most of the reasons for the extra length were surgical difficulties such as thick abdominal wall, pelvic adhesion, less cooperation of patients in early period of this study. (5) Hospital stay after operation in L group required $3{\sim}4$ hours in 125 cases, $2{\sim}3$ hours in 41 cases, $4{\sim}5$ hours in 32 cases out of 250. Maximum 8 hours, minimum 1 hour and average 3.8 hours. In M group hospital stay required $6{\sim}7$ hours in 100 cases, over 7 hours in 85 cases, $5{\sim}6$ hours in 46 cases and so on. Maximum 14 hours, minimum 2 hours and average 6.5 hours. (6) The time between operation and gas passing in the majority cases of both groups, were $12{\sim}36$ hours. A veragetime 20.3 hours in L and 27.2 in M. (7) Laparoscopic sterilization coincident with induced abortion were carried out in 27 cases, laparoscopy with minilaparotomy to control for mesosalpingeal hemorrhage in 1 case. Minilaparotomy coincident with induced abortion were performed in 65 cases, D and C whit polypectomy, menstrual regulatian, and remaval of IUD in 1 case respectively. (8) In L group, 1 case of mesosalpingeal hemorrhage, 1 case of abdominal wall infection were complicated during operation. In M group, 1 case of uterine perfaration, 1 case of abdominal wall infection, 1 case of hemorrhage from omentum and 1 case of bloody vaginal discharge were complicated. No intensive medical treatment was required for those minor complications in both groups. (9) No failure has been recognized and these two sterilization techniques might be the simple, safe and the most effective method for permanent contraception at present time. There is no significant clinical defference between L and M group in this study.