• KSBB Journal
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• v.5 no.2
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• pp.141-149
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• 1990

$\beta$-glucosidase derived from Aspergillus niger was immobilized by (1) covalent linkage on chitin and chitosan with glutaraldehyde, (2) adsorption on DEAE-cellulose and Amberite IRA93 after succinylation, and (3) entrapment on alginate and polyacrylamide gels with various cross linking agents. The retention yield of $\beta$-glucosidase immobilized on chitosan was 31.5% and operational stability was 69% after continuous operation at column reactor(5$0^{\circ}C$ at pH 4.8) for 15 days. The retention yield and operational stability were 24.7% and 60% respectively, in adsorption on Amberite IRA 93. On the other hand, the entrapment method by alginate and polyacrylamide gel was identified to be not appropriate due to the continuous elution of inlmobilized $\beta$-glucosidase. Optimum conditions for the immobilization on chitosan were also studied with optimum pH of 4.8 and glutaraldehyde concentration of 0.4%(w/v). The properties and stability of immobilized $\beta$-glucosidase are also investigted. The conversion yield of cellobiose to glucose was also analyzed using the column type enzyme reactor to evaluate the effectiveness of immobilized enzyme.

• YAKHAK HOEJI
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• v.57 no.4
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• pp.282-288
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• 2013

Zinc oxide nanoparticles (ZnONPs) are widely used in a variety of products and cosmetic products including paper, paints, plastics and sunscreen. However, information on the safety of ZnONPs are not enough and many publications suggest possible toxic effects on environmental and human health. Furthermore, physico-chemical characteristics of nanoparticles makes it hard to test toxicity using the test guidelines of chemicals adopted by regulatory bodies. In this study, stability of ZnONPs was investigated using different types of isotonic solution, which is important in the toxicity study of intravenous route. Precipitation, aggregation, size, zeta potential and morphology of ZnONPs were evaluated with different times and concentrations. Precipitation of ZnONPs were observed in ionic isotonic solution including phosphate-buffered saline, Kreb's-Ringer solution, physiological salt solution and cell culture media of DMEM (Dulbecco's Modified Eagle's Medium) with 10% fetal bovine serum. On the other hand, they were stable without precipitation in non-ionic isotonic solution such as 5% glucose and 2% glycerol, respectively, which are biocompatible for intravenous injection. The average size of ZnONPs in 5% glucose and 2% glycerol was stably maintained, which is less than 30 nm and very similar as that in water dispersion of ZnONPs, provided by the manufacturer. The stability was maintained during the experimental period of 5 days and diluted state up to 15,000 ppm. These data suggest that 5% glucose and 2% glycerol solution can be used for the vehicles of ZnONPs in the toxicity study of intravenous injection route.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.591-595
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• 2010

Conventional methods of model evaluation usually rely only on model performance based on a comparison of simulated variables to corresponding observations. However, this type of model evaluation has been criticized because of its insufficient consideration of the various uncertainty sources involved in modeling processes. This study aims to propose an extended model evaluation method using multiple assesment indices (MAIs) that consider not only the model performance but also the model structure and parameter uncertainties in rainfall-runoff modeling. A simple reservoir model (SFM) and distributed kinematic wave models (KWMSS1 and KWMSS2 using topography from 250m, 500m, and 1km digital elevation models) were developed and assessed by three MAIs for model performance, model structural stability, and parameter identifiability. All the models provided acceptable performance in terms of a global response, but the simpler SFM and KWMSS1 could not accurately represent the local behaviors of hydrographs. In addition, SFM and KWMSS1 were structurally unstable; their performance was sensitive to the applied objective functions. On the other hand, the most sophisticated model, KWMSS2, performed well, satisfying both global and local behaviors. KMSS2 also showed good structural stability, reproducing hydrographs regardless of the applied objective functions; however, superior parameter identifiability was not guaranteed. Numerous parameter sets could lead to indistinguishable hydrographs. This result supports that while making a model complex increases its performance accuracy and reduces its structural uncertainty, the model is likely to suffer from parameter uncertainty. The proposed model evaluation process can provide an effective guideline for identifying a reliable hydrologic model.

• YAKHAK HOEJI
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• v.37 no.4
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• pp.370-382
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• 1993

In order for formulation of rectal containing OMZ, the OMZ suppositories were prepared using water-soluble base, PEG 4000 base and oil-soluble base, Witepsol H 15. Chemical stability of OMZ in suppositories was increased when Witepsol H 15 was used as a suppository base and arginine was added as a stabilizer. The decomposition of OMZ in suppository bases followed the first-order kinetics and their rate constants were 0.11 day $^{1}(t_{1/2}$=/6.25 days) for Witepsol H 15 suppository and 0.48 day $^{1}(t_{1/2}$=/1.43 days) for PEG 4000 suppository, respectively. On the other hand, the decomposition rate constants of Witepsol suppository and PEG suppository stabilized with arginine were 3.89$\times$10$^{-3}$(t$_{1/2}$=171.1 days) and 8.76$\times$10$^{-3}$ day $^{1}(t_{1/2}=79.9 days), respectively. Shelf-lives of the Witepsol and PEG suppositories stabilized with arginine were t$_{90%}$=291.8 days and t$_{90%}$=282.1 days at $35^{\circ}C$ and 75% RH, respectively. The dissolution test of OMZ suppositories was performed by rotating dialysis cell(RDC) method and the release rate constant was calculated by the simplified Higuchi's equation, Q'=K' t$^{1/2}$. Dissolution of OMZ from suppositories was augmented as arginine was added, particle size of OMZ was reduced and a suitable surfactant such as SLS was added. RDC method was more appropriate and available than Paddle method to evaluate the dissolution rate of lipophilic-base suppositoies. Arginine was found to be a very useful exipient for the enhancement of stability and dissolution of OMZ in suppositories.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.1036-1043
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• 2019

Objective: To investigate the color and oxidative stabilities of longissimus lumborum (LL) and psoas major (PM) muscles from grain-finished Bos indicus cattle in Brazil. Methods: The LL and PM muscles were obtained 24 h post-mortem from eight (n = 8) Nellore bull carcasses, fabricated into 1.5-cm steaks, aerobically packaged, and stored at $4^{\circ}C$ for nine days. Steaks were analyzed for myoglobin concentration, pH, instrumental color, metmyoglobin reducing activity (MRA) and lipid oxidation. Results: The LL steaks exhibited greater (p<0.05) redness, color stability, and MRA than their PM counterparts on days 5 and 9. The LL and PM steaks demonstrated similar (p>0.05) lightness and yellowness on days 0, 5, and 9. On the other hand, PM steaks exhibited greater (p<0.05) myoglobin concentration, pH, and lipid oxidation than their LL counterparts. Conclusion: These results indicated that muscle source influenced the color and oxidative stabilities of beef from grain-finished Bos indicus animals. These results highlighted the necessity of muscle-specific strategies to improve the color stability of beef from grain-fed Bos indicus cattle.

• Food Engineering Progress
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• v.23 no.2
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• pp.87-93
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• 2019

Temperature distribution studies were performed in steam-air retort to investigate the influence of various processing conditions (come-up time, sterilization temperature, and internal pressure throughout the steam-air retort). Retort temperature data were analyzed for temperature deviations during holding phase, maximum temperature difference between test locations at the beginning and after 1, 3, and 5 min of the holding phase, and box-and-whiskers plots for each location during the holding phase. The results showed that high sterilization temperature led to a more uniform temperature distribution than low sterilization temperature (pasteurization). In pasteurization condition, the temperature stability was slightly increased by increasing pressure during the holding phase. On the other hand, the temperature stability was slightly decreased in high sterilization temperature condition. Programming of the come-up phase did not affect the temperature uniformity. In addition, the slowest cold spot was found at the bottom floor during the holding phase in all conditions. This study determined that the temperature distribution is affected by retort processing conditions, but the steam-air retort needs more validation tests for temperature stability.

• Journal of the Korea Fashion and Costume Design Association
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• v.24 no.2
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• pp.1-10
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• 2022

The purpose of this study is to analyze the structural properties of 100% wool fabrics knitted with various stitch types and to evaluate dimensional stability from shrinkage in wet cleaning and drying. Materials were weft-knitted from twenty-four different stitches with 7 gauge using a computerized flatbed knitting machine. Weight, thickness, density, and length were measured. A domestic washing machine and a tumble dryer were used for the shrinkage test. The results are as follows: Knitted fabrics were divided into 3 groups based on weight per unit area. Porous knits show light weight whilst milano, pintuck, rib stitches belong to the heaviest group. A positive correlation between weight and thickness was found and the same result was obtained for wale density and weight. Dimensional shrinkage of knitted fabrics was increased during repetitive wet cleaning and drying regardless of knit stitches. Especially, fabrics knitted with float, tuck, cable, and links & links stitches samples were contracted more than 15% in the first treatment whereas 2x1 rib stitch showed 1% shrinkage rate. Fisherman and milano stitches contracted in both course and wale direction with similar shrinkage rates. However, porous knits with float and tuck stitches shrank in course direction by 20% as well as cable samples contracted from 5% to 20% after repeated washing and drying. On the other hand, 30% and 15% contraction of wale direction occurred in orderly float and links & links stitches, respectively. Machine dried knits have a higher shrinkage rate than air-dried knits, but the drying method did not affect to the direction of contraction. In conclusion, variations of knit, tuck, and float stitches affect knit construction and dimensional stability from shrinkage in wet cleaning and drying of wool knitted fabrics.

• Molecules and Cells
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• v.46 no.6
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• pp.374-386
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• 2023

Thermal stress induces dynamic changes in nuclear proteins and relevant physiology as a part of the heat shock response (HSR). However, how the nuclear HSR is fine-tuned for cellular homeostasis remains elusive. Here, we show that mitochondrial activity plays an important role in nuclear proteostasis and genome stability through two distinct HSR pathways. Mitochondrial ribosomal protein (MRP) depletion enhanced the nucleolar granule formation of HSP70 and ubiquitin during HSR while facilitating the recovery of damaged nuclear proteins and impaired nucleocytoplasmic transport. Treatment of the mitochondrial proton gradient uncoupler masked MRP-depletion effects, implicating oxidative phosphorylation in these nuclear HSRs. On the other hand, MRP depletion and a reactive oxygen species (ROS) scavenger non-additively decreased mitochondrial ROS generation during HSR, thereby protecting the nuclear genome from DNA damage. These results suggest that suboptimal mitochondrial activity sustains nuclear homeostasis under cellular stress, providing plausible evidence for optimal endosymbiotic evolution via mitochondria-to-nuclear communication.

• Journal of the Korea Society of Computer and Information
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• v.19 no.8
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• pp.35-44
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• 2014

This paper proposes a 3D pointing interface that is designed for the efficient application of a hand mouse. The proposed method uses depth images to secure high-quality results even in response to changes in lighting and environmental conditions and uses the normal vector of the palm of the hand to perform 3D pointing. First, the hand region is detected and tracked using the existing conventional method; based on the information thus obtained, the region of the palm is predicted and the region of interest is obtained. Once the region of interest has been identified, this region is approximated by the plane equation and the normal vector is extracted. Next, to ensure stable control, interpolation is performed using the extracted normal vector and the intersection point is detected. For stability and efficiency, the dynamic weight using the sigmoid function is applied to the above detected intersection point, and finally, this is converted into the 2D coordinate system. This paper explains the methods of detecting the region of interest and the direction vector and proposes a method of interpolating and applying the dynamic weight in order to stabilize control. Lastly, qualitative and quantitative analyses are performed on the proposed 3D pointing method to verify its ability to deliver stable control.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.11
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• pp.1715-1718
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• 2010

The oxidative stability of grape seed oils (GSOs) prepared from grape seeds roasted at different temperatures (100, 150 and $200^{\circ}C$ for 1 hr) was evaluated and compared with that of GSO from unroasted grape seed. Stability of the GSOs stored in air at $50^{\circ}C$ up to 40 days was assessed by acid value (AV) and peroxide value (PV). Simultaneously, the cont ents of tocopherols and tocotrienols and color changes were monitored up to 40 days. During the storage period, the PV of the unroasted GSO increased from 1.95 to 90.72 meq/kg. On the other hand, the PV for GSOs roasted at 100, 150 and $200^{\circ}C$ increased from 1.96, 2.03, 1.98 to 76.09, 71.72, 49.38 meq/kg, respectively. AV is in conformity with PV. Color development of GSOs increased as roasting temperature increased. The contents of tocopherols and tocotrienols in GSOs increased as roasting temperature increased from 100 to $200^{\circ}C$. The contents of tocopherols and tocotrienols gradually decreased along with the storage period. These results suggest that roasting treatment prolongs the oxidative stability of GSOs.