• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.579-589
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• 2019

Due to the ban on the use of antibiotics, interest has been increasing for the development of therapeutic agents to treat various diseases using natural resources. Osthole, a natural coumarin compound used in traditional Chinese medicines, exerts an anti-inflammatory effect, but its effects in cows remain unknown. In this study, the effect of osthole on lipopolysaccharide (LPS)- or concanavalin-A (Con-A)- stimulated peripheral blood mononuclear cells (PBMCs) was assessed. Jugular venous blood was collected from Korean calves, and PBMCs were isolated. They were then used to study the immune response of PBMCs to treatment with osthole and LPS or Con-A for 72 h by measuring inflammatory cytokines including tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$) and interferon-${\gamma}$ ($IFN-{\gamma}$). Osthole significantly inhibited the mRNA secretion of $TNF-{\alpha}$ and $IFN-{\gamma}$ in a dose-dependent manner. Therefore, osthole inhibited LPS- or Con-A- induced $TNF-{\alpha}$ and Con-A-induced $IFN-{\gamma}$ production significantly in dose-dependent manner. These results clearly suggest that osthole inhibited the LPS- or Con-A- stimulated upregulation of pro-inflammatory cytokines in a dose-dependent manner, without causing obvious cytotoxic effects. Osthole could also protect cows from LPS- or Con-A- induced endotoxin shock, possibly by inhibiting the production of pro-inflammatory cytokines, which suggests that osthole might be a novel therapeutic agent for the prevention of inflammatory diseases.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.111-122
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• 2019

The mulberry tree (Morus alba L.) has been traditionally used in Chinese medicine to treat inflammatory diseases. We investigated the effects of bioconversion on different components of the mulberry tree, and determined changes in the physiological activities. Ethyl acetate-soluble fractions of five different segments (fruit, Mori Fructus; leaf, Mori Folium; twig, Mori Ramulus; root, Mori Cortex; and mistletoe, Loranthi Ramulus) of the mulberry tree show enhanced anti-oxidant effects in the 2,2-diphenyl-1-picrylhydrazyl, and 2,2'-azinobis-(3-ethylvenzothiazoline-6-sulfonic acid) assays, and enhanced anti-inflammatory effects of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in RAW 264.7 macrophages, after being treated with a crude enzyme extract from Aspergillus kawachii, in the following order of activity: Mori Folium>Mori Cortex>Mori Ramulus>Mori Fructus>Loranthi Ramulus. Ethyl acetate- soluble fraction of mulberry leaves (Mori Folium) that underwent bioconversion was most effective, and was devoid of any cytotoxicity. The fraction was also effective against mRNA expression of LPS-induced pro-inflammatory cytokines, such as inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis $factor-{\alpha}$, $interleukin-1{\beta}$, and interleukin-6. In addition, the fraction was effective in LPS-induced phosphorylation of mitogen-activated protein kinases and IKK, and $I{\kappa}B$ degradation, followed by translocation of the nuclear $factor-{\kappa}B$ from the cytoplasm to the nucleus. Thus, bioconversion increased the anti-oxidative and anti-inflammatory activities of the mulberry leaf.

• Korean Journal of Heritage: History & Science
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• v.43 no.2
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• pp.82-119
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• 2010

"Gongchengzuofazeli" was published by the Ministry of Engineering (工部) in 1734, the 12th year of the Yongzheng(雍正) emperor of China's Ching Dynasty. Its purpose was the standardization of building construction and the strengthening of management policies The book records exact measurements of every material used in the building, dividing the building into sections called "dugu (斗口)." It also contains records of the funds that were needed for fair management. Today, it has great historical value because it provides information as to the state of architectural technology and environment of that time. However, the research of Korean architectural historians tends to focus on the "Yingzaofashi(營造法式)" which was written in the Northen Song Dynasty. While the significance of "Gongchengzuofazeli" is widely acknowledged, not enough interest has been garnered to even begin a full-scale translation project. This article, by summarizing in the introduction the basic contents of "Gongchengzuofazeli" and a translation of the first volume in the body, seeks as its purpose to provide data to become the foundation of future research in this area.

• Korean Journal of Heritage: History & Science
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• v.44 no.2
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• pp.26-45
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• 2011

The Gongchengzuofazeli, which was published in 1734, the 12th year of the Yongzheng (雍正) emperor of the Ching Dynasty, is an authoritative book that discusses the architectural technology and engineering practices at that time. Moreover, buildings constructed during the Ching Dynasty are substantially similar with the book's content. This study is important in that it is done as the first step in conducting a comparative analysis between the Korean and Chinese wood structure mechanism. First, each content of the book is examined according to the editing order. And second, the standard for division between Da-shi (大式) and Xiao-shi (小式) architecture in terms of heavy woodwork method (vol. 1~27) is redefined, from which a new organization is deduced.

• Korean Journal of Heritage: History & Science
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• v.37
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• pp.213-242
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• 2004

"China", "Guang-Xi(廣西)", "Dong-Shan(東山)", a marriage of "Yao-zu(瑤族)" divide mainly into a distinguished family form, but are by a marriage of a traditional general form (a woman getting married) and "Zhaoxu-Hun(招婿婚)", a marriage of "Liangtou-che(兩頭扯)". These two marriage format that mentioned the back is a marriage format to often appear in other Chinese minority race region while being a custom peculiar to "Yao-zu(瑤族)" marriage method. "Zhaoxu-Hun(招婿婚)" provides labor force as a workman while living with a man sinks with "son-in-law" to a woman house, and doing, and it is to carry out a lot of duty as a husband too. "A Liangtou-che(兩頭扯)" marriage is more characteristic, but lives while going around a bilateral family while man and woman each stay in an own house. There are inheritance of property rights to own family, too and obeys anger of he direction family bilateral where if lays a child even if stands up and holds a memorial service. At the same time that these marriages are performed between the same "Yao-zu(瑤族)" near a track, and solution does a distribution problem between a labor problem and the families which were unstable through a mutual marriage for the purpose a compunction "Yao-zu(瑤族)" social network construction into intimacy anger. A general idea is covered he base with in order the porcelain which is a weak race prevent that it is assimilated by external aggression because "Yo-zu(瑤族)" has always received aggression of "a Han-zu(漢族)" too historically, and to keep an ethnic pure blood.

• Korean Journal of Ecology and Environment
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• v.52 no.2
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• pp.171-177
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• 2019

Smart farm is a breakthrough technology that can maximize crop productivity and economy through efficient utilization of space regardless of external environmental factors. This study was conducted to investigate the optimal growth and physiological conditions of Chinese matrimony vine (Lycium chinense) with LED light sources in a smart farm. The light source was composed of red+blue and red+blue+white mixed light using a LED system. In the red+blue mixed light, red and blue colored LEDs were mixed at ratios of 1:1, 2:1, 5:1, and 10:1, with duty ratios varied to 100%, 99%, and 97%. The experimental results showed that the photosynthetic rate according to the types of light sources did not show statistically significant differences. Meanwhile, the photosynthetic rate according to the mixed ratio of the red and the blue light was highest with the red light and blue LED ratio of 1:1 while the water use efficiency was highest with the red and blue LED ratio of 2:1. The photosynthetic rate according to duty ratio was highest with the duty ratio of 99% under the mixed light condition of red+blue+white whereas the water use efficiency was highest with the duty ratio of 97% under the mixed light of red+blue LED. The results indicate that the light source and light quality for the optimal growth of Lycium chinense in the smart farm using the LED system are the mixed light of red+blue (1:1) and the duty ratio of 97%.

• The Journal of Internal Korean Medicine
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• v.40 no.1
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• pp.89-116
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• 2019

Objective: This study aimed to ascertain what should be considered in the "Guideline for Clinical Trials with Herbal Medicinal Products for Liver Cancer," by analyzing existing guidelines and clinical trials. Methods: Committee for the development of a guideline, consisting of 6 Korean medicine doctors, reviewed guidelines and clinical trials on using herbal medicine for treating liver cancer. The trials were analyzed in terms of inclusion and exclusion of participants, intervention, comparators, outcomes, and trial design. We then compared the results of our analysis with the guidelines to identify issues we must to consider when following the "Guideline for Clinical Trials with Herbal Medicinal Products for Liver Cancer." Several guidelines for antitumor agents and clinical trials on herbal medicine were obtained from the Ministry of Food and Drug Safety homepage, etc. The search terms were as follows: "liver neoplasms"; "herbal medicine"; "medicine, Korean traditional"; and "medicine, Chinese Traditional.". Results: Ten articles were obtained from pubmed and Embase. There was no guideline for clinical trials on using herbal medicine for treating liver cancer. All the participants in the reviewed articles had primary liver cancer, and the type of intervention varied (e.g., decoction, patches, and capsules. The comparators included placebos and conventional treatments such as chemotherapy. The outcome assessment methods were tumor response, quality of life, survival, and liver function tests. Adverse events occuring during the trial were also evaluated. Conclusion: Findings were derived by reviewing existing guidelines and comparing them with clinical trials on liver cancer and herbal medicinal products. These results will be utilized in the development of the "Guideline for Clinical Trials with Herbal Medicinal Products for Liver Cancer."

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.57-66
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• 2019

The ethyl acetate fraction of Bat Faeces (Ye Ming Sha: natural products used in Chinese Medicine) after fermentation (EFBF-AF) showed enhanced anti-oxidative effects in 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assays. Fermentation of the Bat Faeces by using the crude enzyme extract from Aspergillus kawachii, significantly increased the anti-inflammatory effects. Fermented Bat Faeces markedly inhibited nitric oxide production, inducible nitric oxide synthase, and cyclooxygenase-2 expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The EFBF-AF reduced the nuclear translocation of nuclear factor kappa B ($NF-{\kappa}B$) via $IKK{\alpha}$ and $I{\kappa}B{\alpha}$ phosphorylation, and decreased the phosphorylated the extracellular signal-regulated kinases (ERK) and p38 expression in LPS-treated RAW 264.7 macrophages. In addition, the EFBF-AF suppressed the expression of pro-inflammatory genes, such as interleukin-$1{\beta}$, interleukin-6, and tumor necrosis $factor-{\alpha}$. These results suggest that fermented Bat Faeces may suppress pro-inflammatory responses in LPS-stimulated RAW 264.7 macrophages cells via ERK, p38 mitogen-activated protein kinase and $NF-{\kappa}B$ signaling pathways.

• Biomolecules & Therapeutics
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• v.27 no.2
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• pp.216-221
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• 2019

The c-Met protein is a receptor tyrosine kinase involved in cell growth, proliferation, survival, and angiogenesis of several human tumors. Overexpression of c-Met has been found in gastric cancers and correlated with a poor prognosis. Indirubin is the active component of Danggui Longhui Wan, which is a traditional Chinese antileukemic recipe. In the present study, we tested the anti-cancer effects of an indirubin derivative, LDD-1937, on human gastric cancer cells SNU-638. When we performed the in vitro kinase assay against the c-Met activity, LDD-1937 inhibited the activity of c-Met. This result was confirmed by immunoblot and immunofluorescence of phosphorylated c-Met. Immunoblot analysis showed that LDD-1937 decreased the expression of the Erk1/2, STAT3, STAT5, and Akt, downstream proteins of c-Met. In addition, LDD-1937 reduced the cell viability and suppressed colony formation and migration of SNU-638 cells. Furthermore, LDD-1937 induced $G_2/M$ phase arrest in the SNU-638 cells by decreasing the expression levels of cyclin B1 and CDC2. Cleaved-PARP, an apoptosis-related protein, was up-regulated in cells treated with LDD-1937. Overall, this study suggests that LDD-1937 may be a novel small-molecule with therapeutic potential for selectively inhibiting c-Met and c-Met downstream pathways in human gastric cancers overexpressing c-Met.

• Journal of Information Technology Applications and Management
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• v.25 no.4
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• pp.41-65
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• 2018

Corporate Social Responsibility (CSR) activities are important tool in corporate management strategies. However, the way that companies conduct CSR activities might bring different effect on consumers. The purpose of this study is to verify the effect of CSR fit (similarity between the firm's CSR activities and the firm's core products or technologies) on the consumers' evaluation on product performance. In addition to the main effects of CSR fit on perceived product performance, and we also examined how this effect changes when CSR information source and consumer expertise are involved as control variables. Study 1 of this research was conducted with US consumers and study 2 was with Chinese consumers. Experimental results show that consumers' perceive product performance is better when the fit between CSR activities and firm's core products or technologies is low than the fit is high. When CSR information sources are neutral sources, consumers perceive product performance to be better when the fit between the firm's CSR activities and the firm's core products or technologies is low than the fit is high. However when CSR information was delivered by company's source like nadvertisement, such difference disappears. This study also show that the higher the customer's expertise, the better the product performance was perceived for high-fit CSR activities. On the other hand, the lower the consumer's expertise, the better perceived product performance was perceived for low-fit CSR activities.