• Biomolecules & Therapeutics
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• 제31권5호
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• pp.550-558
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• 2023

Hair loss is a common condition that can have a negative impact on an individual's quality of life. The severe side effects and the low efficacy of current hair loss medications create unmet needs in the field of hair loss treatment. Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1), one of the components of the extracellular matrix, has been shown to play a role in maintaining its integrity. HAPLN1 was examined for its ability to impact hair growth with less side effects than existing hair loss treatments. HAPLN1 was predominantly expressed in the anagen phase in three stages of the hair growth cycle in mice and promotes the proliferation of human hair matrix cells. Also, recombinant human HAPLN1 (rhHAPLN1) was shown to selectively increase the levels of transforming growth factor-β receptor II in human hair matrix cells. Furthermore, we observed concomitant activation of the ERK1/2 signaling pathway following treatment with rhHAPLN1. Our results indicate that rhHAPLN1 elicits its cell proliferation effect via the TGF-β2-induced ERK1/2 pathway. The prompt entering of the hair follicles into the anagen phase was observed in the rhHAPLN1-treated group, compared to the vehicle-treated group. Insights into the mechanism underlying such hair growth effects of HAPLN1 will provide a novel potential strategy for treating hair loss with much lower side effects than the current treatments.

• 대한화장품학회지
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• 제48권4호
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• pp.385-392
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• 2022

본 연구에서는 녹용에 함유한 단백질인 케라틴을 Fervidobacterium islandicum AW-1로 고온 혐기 발효를 통하여 케라틴 펩타이드를 생산하고, 인체 모발 관련한 인자를 확인하여, 화장품 원료로서 케라틴 펩타이드의 가능성을 확인하였다. 모유두 세포주에서 녹용 발효 케라틴 펩타이드를 농도에 따라 세포 독성 및 증식을 확인한 결과, 세포 독성은 나타나지 않았고, 세포 증식 효과를 보였다. 인체 모유두 세포에 대하여 녹용 발효 케라틴 펩타이드에 따른 성장인자의 증가를 확인하여 모발에 영향을 준다고 판단하였다. 이러한 녹용 발효 케라틴 펩타이드를 함유한 화장품을 제조하여 피부 안전성 및 탈모 증상 완화 인체 효력 시험을 수행한 결과, 사용 12 주 후에 제품 사용 전 대비 전체 모발 수의 증가 및 대조군과 비교시 전체 모발 개수의 차이가 통계적으로 유의하게 나타났다. 이를 통해 녹용 발효 케라틴 펩타이드는 기능성 화장품 원료뿐만 아니라 건강 기능 식품 소재로의 가능성을 확인하였다.

• 생명과학회지
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• 제18권4호
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• pp.542-549
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• 2008

Cisplatin은 임상적으로 다양한 종류의 종양 치료에 사용되는 중요한 항암제 중의 하나이다. 그러나 cisplatin은 이독성, 신장독성, 골수독성, 위장독성 및 말초신경독성 등의 심각한 부작용으로 인하여 사용이 제한적이다. Cisplatin에 의한 청각장애에서 organ of Corti 외측 유모세포(outer hair cells) 손상이 유발한다. Cisplatin에 의한 세포독성에 대한 연구가 진행 중이지만 pro-inflammatory cytokine과 관련된 청각세포사멸에 대한 연구는 미비하다. 이 연구에서 cisplatin은 청각세포주 HEI-OC1 세포와 렛트 cochlear explant에서 염증성 사이토카인인 $TNF-{\alpha}$, $IL-1{\beta}$ 및 IL-6의 유전자 발현과 분비를 현저히 증가시켰다. 이들 염증성 사이토카인은 organ of Corti 청각유모세포에 직접적인 세포독성을 나타내어 외측 및 내측 유모세포와 배열을 파괴하였다. 염증반응에서 중요한 $TNF-{\alpha}$를 cisplatin을 처리한 실험군에서 immunocytochemistry를 통하여 관찰 한 결과 organ of Corti에서의 발현이 현저히 증가됨을 관찰하였다. 염증성 사이토카인에 대한 중화항체를 처리하여 cisplatin에 의한 세포독성이 현저히 감소됨을 HEI-OC1 세포와 청각유모세포에서 확인하였다. 또한 GSH, NAC와 같은 항산화제를 처리하여 세포독성이 현저히 감소됨을 확인하였다. 이상의 결과는 cisplatin에 의한 청각유모세포의 죽음에서 $TNF-{\alpha}$, $IL-1{\beta}$ 및 IL-6와 같은 염증성 사이토카인이 병리 생리학적으로 중요한 역할을 하고 있음을 시사한다.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.74-94
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• 2016

Objectives : YangHyulEum Gami-Bang(YHEG) is a hair care extracts which is composed of fourteen plant extracts used in oriental medicine. The purpose of this study is to investigate the effect of YangHyulEum Gami-Bang(YHEG) on the alopecia and hair growth.Methods & Results : The herbal extracts from YangHyulEum Gami-Bang(YHEG) was tested using in vivo and in vitro test models. 1. The YHEG extracts showed effect on the DNA proliferation of the hair dermal papilla cells measured by [3H]thymidine incorporation. 2. YHEG showed promoting on the expression of growth factors such as IGF-1, KGF-1 and inhibiting on the expression of inhibitory hair growth factor such as TGF-β1, BMP-2 estimated by qPCR. 3. The YHEG extracts showed effect on the activation of β-catenin in the dermal papilla cells. 4. YHEG showed inhibitory effects of NO synthesis at 0.2% concentrations. 5. YHEG showed effects in the expression of IL-1β, TNF-α, IL-6, COX-2 and iNOS gene in the LPS stimulated RAW 264.7 cells. 6. The hair growth index of the YHEG extracts ranked at over 2 when compared to control group which was ranked at 0. 7. The hair follicle number, length and size of the experimental group were remarkably higher than the control group in the histological observation.Conclusions : These results suggest that YangHyulEum Gami-Bang(YHEG) has hair growth promoting activity and it can be used as a potent treatment agent for preventing hair loss and stimulating hair growth for treatment of alopecia.

• 한국미생물·생명공학회지
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• 제51권1호
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• pp.43-52
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• 2023

The purpose of this study is to confirm the antioxidant, black hair, and hair growth effects of the N. jatamansi, O. basilicum, and C. sativus mixed extracts that pharmacological efficacy has been verified. Accordingly, four samples (NOC-1 to-4) produced under different extraction conditions were prepared and the results are as follows. First, all samples showed an increase in antioxidant content in a concentration-dependent manner from the results of antioxidant efficacy. In particular, NOC-4 extracted by steaming and ultrasonic methods showed the highest antioxidant effect among the four samples. As a result of analysis of the amount of melanin production in mouse melanoma cells, NOC-4 with concentration of 500 ㎍/ml showed higher melanin production compared to the control group, so the black hair efficacy was the best. Also, in the hair growth test results, it was found that the hair growth was the best at 0.94 ± 0.10 mm at experimental group orally administered with 500 mg/kg of NOC-4. In addition, as a result of cytotoxicity analysis in mouse melanoma cells, the safety of samples was demonstrated by maintaining cell viability of 95% or more at all concentrations. These results suggest that the steaming and ultrasonic extraction method increased the extraction yield of active ingredients for antioxidant, melanin, and hair generation, thereby affecting physiological activity. Based on these results, if the steaming and ultrasonic extraction methods are applied to the mixed extraction of N. jatamansi, O. basilicum, and C. sativus, it is judged that the practical potential as a natural material for black hair and hair growth agents will increase.

• 치위생과학회지
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• 제23권4호
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• pp.369-377
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• 2023

Background: In this study, we investigated the potential use of keratin for oral tissue regeneration. Keratin is well-known for its effectiveness in skin regeneration by promoting keratinization and enhancing the elasticity and activity of fibroblasts. Because of its structural stability, high storability, biocompatibility, and safety in humans, existing research has predominantly focused on its role in skin wound healing. Herein, we propose using keratin proteins as biocompatible materials for dental applications. Methods: To assess the suitability of alpha-keratin protein as a substrate for cell culture, keratin was extracted from human hair via PEGylation. Viabilities of primary human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) were assessed. Fluorescence immunostaining and migration assays were conducted using a fluorescence microscope and confocal laser scanning microscope. Wound healing and migration assays were performed using automated software to analyze the experimental readout and gap closure rate. Results: We confirmed the extraction of alpha-keratin and formation of the PEG-g-keratin complex. Treatment of HGFs with keratin protein at a concentration of 5 mg/ml promoted proliferation and maintained cell viability in the test group compared to the control group. HOKs treated with 5 mg/ml keratin exhibited a slight decrease in cell proliferation and activity after 48 hours compared to the untreated group, followed by an increase after 72 hours. Wound healing and migration assays revealed rapid closure of the area covered by HOKs over time following keratin treatment. Additionally, HOKs exhibited changes in cell morphology and increased the expression of the mesenchymal marker vimentin. Conclusion: Our study demonstrated the potential of hair keratin for soft tissue regeneration, with potential future applications in clinical settings for wound healing.

• 생약학회지
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• 제49권1호
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• pp.7-14
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• 2018

We isolated gallotannin, 2,6-digalloyl-1,5-anhydroglucitol, known as acertannin (1), from the barks and xylems of Acer ginnala Maxim. It is a genus of Acer species of shrubs in the family Aceraceae. A. ginnala grows in Korea, Japan and Mongolia. We accomplished the structure elucidation by confirming that the result of $^1H$,$^{13}C-NMR$,MS spectrum data was similar to previous references. We measured DPPH and ABTS radical scavenging activity in vitro to evaluate anti-oxidative activities on acertannin isolated from A. ginnala. Acertannin from A. ginnala exhibited potent DPPH and ABTS radical scavenging activities. We examined the antioxidant and apoptosis modulative effects. This examination shows that A. ginnala has not only 1,1-diphenyl-2-picryhydrazyl(DPPH) radical scavenging activity and ABTS radical scavenging activity, but also human hair dermal papilla cell protection effects. These results indicate that the barks and xylems of A. ginnala might be developed as a potent anti-oxidant, hair growth agent, and ingredient for related new functional cosmetic materials.

• 대한의용생체공학회:의공학회지
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• 제14권2호
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• pp.125-136
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• 1993

The study of Cochlear biomechanics is to clearly define three biomechanical principles of the Cochlea : Activity, Nonlinearity and Feedback. In this article, the Cochlea is linearly and actively modelled in one dimensional time domain. The sharp tunning of the Basilar Membrane displacement is shown when the amplifying activity of hair cells is added to the model. The amplified energy of the travelling displacement wave is emitted throughout the Cochlear fluid, so that the model becomes unstable. A new technique is introduced to reduce strong echos fro the Helicotrema. It makes the model less unstable. Both pure and click tones are used as input stimuli onto the ear durm. When the model is normal, the click response of the model shows that the backward emission of the amplified fluid pressure has mainly the echos from the Helicotrema. However, when the linear and active model is assumed to be abnormal, that is, some of hair cells are damaged not to produce the active process, the effect of the hair cell damage is resulted in the Oto-acoustic emission. The frequency response of the abnormally emitted sound pressure shows that the Oto-acoustic emission has the information about the characteristic frequency of the damaged hair cell. The main aim of this paper is to demonstrate the active biomechanics of the Chchlea in the time domain.

• Applied Microscopy
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• 제38권3호
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• pp.265-273
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• 2008

본 연구는 국내에 거주하는 흑인종, 백인종, 황인종 여성의 모발을 채취하여 전자현미경으로 모발의 미세구조적 차이를 규명하였다. 흑인종 모발의 횡단면은 납작한 타원형의 형태를 하고 있었으며 황인종과 백인종 모발은 각각 원형과 타원형의 형태를 하고 있었다. 흑인종 모발은 두께가 $90{\sim}115{\mu}m$로 굵기가 일정하지 않았다. 황인종모발은 두께가 $100{\mu}m$이었으며 백인종 모발은 두께가 $80{\mu}m$로 측정되었다. 모발의 두께는 황인종 모발이 백인종 모발보다 크게 나타났다. 흑인종 모발은 황인종과 백인종 모발 보다 큐티클층이 많이 손상되어 있었다. 특히 큐티클세포의 내큐티클에는 많은 구멍들이 형성되어 있어서 쉽게 부서지는 특성이 나타났다. 백인종 모발의 피질에 있는 페오멜라닌은 내부에 동심원상의 나선구조를 하고 있었다.

• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1467-1474
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• 2023

Due to the continuous increase in patients with androgenetic alopecia (AGA) and psychological disorders such as depression and anxiety, the demand for hair loss treatment and effective hair growth materials has increased. Terminalia bellirica (Gaertn.) Roxb. (TBE) reportedly exerts anti-inflammatory, hepatoprotective, and antidiabetic effects, among others, but its effects on testosterone (TS)-inhibited hair growth remains unclear. In this study, we evaluated the effects of TBE on TS-induced hair growth regression in human follicle dermal papilla cells (HFDPCs) and C57BL/6 mice. Oral administration of TBE increased TS-induced hair growth retardation. Interestingly, effects were greater when compared with finasteride, a commercial hair loss treatment product. Histological analyses revealed that oral TBE administration increased hair follicles in the dorsal skin of C57BL/6 mice. Additionally, western blotting and immunofluorescence showed that oral TBE administration recovered the TS-induced inhibition of cyclin D1, proliferating cell nuclear antigen (PCNA), and Ki67 expression in vivo. Using in vitro proliferation assays, TBE promoted HFDPC growth, which was suppressed by TS treatment. Thus, TBE may be a promising nutraceutical for hair health as it promoted hair growth in AGA-like in vitro and in vivo models.