• Journal of Ginseng Research
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• v.22 no.3
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• pp.216-221
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• 1998

We examined the anti-invasive activity of ginsenosides Rhl, Rha on the highly metastatic HT1080 human fibrosarcoma cell line. In vitro invasion assay showed ginsenoside Rhr reduced tumor cell invasion through a reconstituted basement membrane in a transwell chamber more than ginsenoside Rh1. Significant down-regulation of matrix metalloproteinase-9 (MMP-9) by ginsenosides Rh, and Rh2 was detected by Northern blot analysis. However, the expression of MMP-2 was not affected by Rh, and Rhr. The expression of tissue inhibitor of metalloproteinase-2 (TIMP-2) was increased by Rhl after 0.5, 1 or 3 day-treatment but reduced after 6 day-treatment. However, the expression of TIMP-2 was not changed by treatment with Rh2. Plasminogen activator inhibitor (PAI) and urokinase-type plasmlnogen activator (uPA) were not changed by treatment with Rh1 and Rh2 for 3 and 6 days. Quantitative gelatin-based zymography confirmed a markedly reduced expression of MMP-9 but MMP-2 after treatments with ginsenosides Rhl and Rha. These results suggest that down-regulation of MMP-9 contributes to the anti-invasive activity of ginsenosides Rhl and Rhr in the HT1080 cells.

• Journal of Ginseng Research
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• v.10 no.1
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• pp.27-35
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• 1986

This study was devised to observed the growth inhibition and change of disaccharidase activities of human colon cancer cells cultured in medium containing the ginseng extract. Three species of human colon cancer cell lines, HRT-18, HCT-48 and HT-29, were used for the experiment. The activities of sucrease, lactase, maltase and trehalase in the cancer cells were determined. The results obtained are summarized as follows; 1. The doubling times of the HRT-18, HT-29 and HCT-48 were about 20,22 and 24 hours, respectively. 2. The growth rates of the HRT-18 and HCT-48 in culture medium containing the ginseng extract were inhibited gradually according to increase of the concentration of ginseng extract and extension of the incubation time. 3. The activities of disaccharidase in HRT-18 and HCT-48 cultured in the medium containing the ginseng extract were increased compared with control group as follows;

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.21 no.2
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• pp.79-84
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• 2015

Pre-drying of 'Fuyu' persimmon was performed right after harvesting from a farm. Pre-drying conditions were varied with room temperature (RT) for 1 day to 7 days, low temperature (LT, at $20{\sim}30^{\circ}C$) for 1 day to 4 days, high temperature (HT, at $30{\sim}40^{\circ}C$) for 3 h to 12 h, and ultra-high temperature (UT, at $50{\sim}60^{\circ}C$) for 30 min to 120 min. Weight loss of pre-dried persimmon was increased from 1.62% up to 2.96% with increased pre-drying temperature and time. Pre-drying at RT resulted more significant weight loss of persimmon compared to that of pre-drying at HT. Minimum firmness loss of persimmon stored at $0^{\circ}C$ for 100 days was obtained at the condition of HT for 6 h. Rate of peel blackening was decreased from 31.5% to 16.4% and 10.9% by pre-drying at HT for 6 h and 9 h, respectively.

• Tribology and Lubricants
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• v.36 no.4
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• pp.244-252
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• 2020

Materials manufactured by powder metallurgy (PM) are widely used in various applications such as water pump, shock absorber, and airplane components due to the reduction in the cost and weight. In this study, tribological properties of carbon steel subjected by surface treatment were investigated. The main purpose is to increase the strength and improve the tribological properties by reducing pores that formed by PM. Moreover, the surface treatment was carried out at room and high temperatures (RT and HT). The surface roughness of the untreated (NON) and treated (AFTER) samples was measured. It was found that the surface roughness was reduced after both the RT AFTER and HT AFTER compared to RT NON sample. The tribological properties of the samples were performed against bearing steel ball under dry conditions. The friction coefficient of the RT NON samples was reduced by 22% and 56% RT AFTER and HT AFTER, respectively. The wear volume of the RT NON sample was also reduced by 43% and 87% RT AFTER and HT AFTER, respectively. Tribocorrosion tests were also performed and it was found that the surface of the RT AFTER, HT AFTER samples was less corroded compared to RT NON sample. The HT AFTER sample demonstrated a relatively higher corrosion potential in comparison with the RT AFTER samples. Hence, it was confirmed that after surface modification the surface roughness and hardness of the samples were significantly improved resulting in improvement in tribological and tribocorrosion behaviors of PM carbon steel.

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.19-32
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• 2005

Objectives : to evaluate the analgesic effect of bee venom acupuncture on Choksamni (ST36) in the collagen-induced arthritis rats and investigate the role played by serotonergic receptor subtypes (5-HT1a, 5-HT2a) in the antinociceptive effect of bee venom acupuncture in a thermal hyperalgesia test Methods : Experiments were performed on 5 week-aged 60 male Sprague-Dawley rats according to National Institute of Health guidelines and the ethical guidelines of the International Association for the Study of Pain (IASP). Arthritis was induced with arthrogenic collagen emulsion (Bovine type II collagen ${\mu}g$ with incomplete Freund's adjuvant $100\;{\mu}g$). The onset of arthritis was considered to be present when erythema and swelling were detected in at least one joint. The thermal hyperalgesia was evaluated weekly with tail flick test in the rats of severity grade 3 without any injury at tail and foot (including inflammation, ulceration, snap). In the fourth week after first immunization, the analgesic effect of bee venom acupuncture (Choksamni, ST36) was measured with consecutive tail flick latency after intraperitoneal injection of spiroxatrine (1mg/kg) and spiperone (1mg/kg). Results : Chronic inflammatory pain was induced as time elapsed after the immunization of arthrogenic collagen and the maximum value was reached from third to fifth week. Chronic inflammatory pain induced by CIA was inhibited by bee venom acupuncture on the left ST36. The analgesic effect of bee venom acupuncture was inhibited by intraperitoneal injection of 5-HT1a antagonist spiroxatrine and 5-HT2a antagonist spiperone. Conclusions : Therefore, a conclusion. that the analgesic effect of bee venom acupuncture in the chronic inflammatory pain is partially mediated by 5-HT1a and 5-HT2a receptors can be made.

• The Journal of Internal Korean Medicine
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• v.22 no.3
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• pp.321-330
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• 2001

Objectives: This study was undertaken to investigate the action mechanism of Holotrichia (HT) at parameter related to dementia. Methods: HT was tested for the effects on acetylchonine esterase and monoamine oxidase activities, lipid peroxidation, antioxidation in brain of lead acetate-treated rats. Lead acetate were treated firstly into samples for 10 days, and then lead acetate and HT were set with them for 20 days. Results: The level of lipid peroxide, acetylcholine esterase and monoamine oxidase activities, enzyme activities and ratio of type conversion of xanthine oxidase increased in lead acetate-treated rats were decreased as highly as normal group by HT. Superoxide dismutase, catalase and glutathione peroxidase activities, the level of acetylcholine decreased in lead acetate-treated rats were increased as lowly as normal group by HT. Conclusions : These results suggest that HT might have an effect on treatment of dementia according to decreasing the activities of acetylcholine esterase, monoamine oxidase and level of lipid peroxide in brain.

• The Journal of Korean Physical Therapy
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• v.16 no.3
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• pp.22-31
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• 2004

The purpose of this study was to demonstrate the effects of silver spike point (SSP) low frequency electrical stimulation on plasma ${\beta}-endorphin$ activities measured by radio- immunoassay from normal volunteer and the effects of ${\beta}-endorphin$ on 5-hydroxytryptamine (5-HT, serotonin)-induced contraction investigated by isometric tension methode in rats. The current of 3 Hz continue type, but not 100 Hz continue type, of SSP low frequency electrical stimulation significantly increased in plasma ${\beta}-endorphin$ from normal volunteer. The endothelial cell-dependent 5-HT-induced contractions were inhibited by ${\beta}-endorphin$ $1{\mu}M$. These results suggest that the ${\beta}-endorphin$ regulates nociceptive-like substance, such as 5-HT, in part and that the SSP low frequency electrical stimulation, specifically current of low frequency of 3 Hz continue type, significantly increases plasma ${\beta}-endorphin$ from normal volunteer.

• Journal of Microbiology
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• v.35 no.2
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• pp.123-126
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• 1997

The aim of the present study was to optimize culture condition for the expression of lipocortin-1$_{1-185}$ in a recombinant of Escherichia coli using batch system. Plasmid (pHT22) carrying lipocortin-1$_{1-185}$ gene was well maintained in the recombinant with the addition of amplicillin as a selection pressures. Optimum temperature was 28$^{\circ}C$ for seed culture and 4$0^{\circ}C$ for main culture and the optimum pH was 7.0. The production of Lipocortin-1$_{1-185}$ was closely associated with cell growth and related to plasmid amplification.

• 한국약용작물학회:학술대회논문집
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• 2012.05a
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• pp.231-232
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• 2012

• Journal of the Korean Society of Industry Convergence
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• v.22 no.2
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• pp.191-199
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• 2019

In this paper, dissimilar friction welding were produced using 15 mm diameter solid bar in chrome molybedenum steel(SCM440) to stainless steel(STS316L) to investigate their mechanical properties. Consequently, optimal welding conditions were n=2000 rpm, HP=70 MPa, UP=140 MPa, HT=10 sec and UT=10 sec when the metal loss(Mo) is 8.6 mm. In addition, an acoustic emission technique was applied to evaluate the optimal friction welding condition. AE parameters including the cumulative count, amplitude and energy showed a various changes according to the friction condition. A continuous type waveforms and low frequency spectrum was presented in friction time. On the other hand, a burst type waveform and high frequency spectrum was exhibited in pressing time.