• Title/Summary/Keyword: HSR

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Tolerance to Potato Soft Rot Disease in Transgenic Potato Expressing Soybean Ferritin Gene (대두 철분결합단백질 유전자 발현 형질전환 감자의 감자무름병 방어 증진효과)

  • Bae, Shin-Chul;Yeo, Yun-Soo;Heu, Sung-Gi;Hwang, Duk-Ju;Byun, Myung-Ok;Go, Seung-Joo
    • Journal of Plant Biotechnology
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    • v.29 no.4
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    • pp.229-233
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    • 2002
  • Ferritin is ubiquitous in bacteria, animals and plants. Ferritin is thought to play two main roles in living cells to provide iron for the synthesis of iron protein such as ferretoxin and cytochromes and to prevent damage from radicals produced by iron/dioxygen interaction. To enhance the resistance of potato to Erwinia carotovora, the soybean ferritin gene was introduced into the potato either under CaMV 35S or hsr203J promoter. Potato transgenic plants were screened by PCR analysis using specific primers to the ferritin gene. Expression of ferritin gene under CaMV 35S and hsr203J promoter in potato transgenic plants was confirmed by northern blot analysis. hsr203J promoter known to pathogen inducible in tobacco drives the induction upon Phytophthora infestan in potato and the transcript level of ferritin gene was extremely high after 24 hours post inoculation. One of transformants under CaMV 35S promoter was increased 2.5 fold than untransformant. Each one of transgenic potato containing gene promoter CaMV 35S and hsr203J-ferrtin fusion exhibited tolerance against potato soft rot.

Analysis of Safety and Mobility of Expressway Land Control System (길어깨차로제 시행에 따른 안전성 및 이동성 분석)

  • Park, Sung-ho;Lee, Yoseph;Kang, Sungkwan;Cho, Hyonbae;Yun, Ilsoo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.20 no.3
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    • pp.1-19
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    • 2021
  • The domastic hard shoulder running(HSR) System has been gradually expanding since its initial implementation in September 2007 with the aim of increasing capacity and resolving congestion. Hard Shoulder is used as a space for driver's visual comfort and a place for vehicles to evacuate in case of emergency, but it is replaced by a space for driving when the HSR System is implemented. Therefore, it was intended to determine the improvement effect before and after implementation of the HSR system through safety analysis and mobility analysis. The safety analysis analyzed the impact of traffic accidents by comparing HSR sections and similar sections. The mobility analysis was to determine the improvement effect by quantifying the speed and traffic volume changes before and after HSR System implementation. According to safety yanalysis, there is no effect of reducing traffic accidents when implementing the HSR System. In mobility analysis, the implementation of the HSR System significantly improved the speed of traffic during peak hours and significantly reduces slow and delay hours.

A HSR Performance Analysis for Distal Substation (디지털 변전소의 HSR 적용을 위한 성능 분석)

  • Lee, Byung-Hoon;Kwak, Jong-Kab;Choi, Gui-Yul;Jang, Hyuk-Soo;Yang, Hyo-Sik;Jang, Byung-Tae;Lee, Nam-Ho
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.284-285
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    • 2011
  • 변전소의 디지털화를 통한 변전소 자동화 시스템(SAS) 구축이 전 세계적 이슈이다. 변전소 디지털화는 기존의 변전소 기능에 정보 통신 기술을 적용시켜 변전소 운영을 효과적으로 수행할 수 있게 돕기 위해서이다. 디지털 변전소가 정상적으로 운영되기 위해 요구하는 높은 수준의 가용성과 전송능력 만족하기 위해 IEC TC 57 WG10은 IEC SC65 WG15의 IEC 62439 고 가용성 자동화 네트워크(Highly Available Automation Networks)를 디지털 변전소 네트워크로 차용하기로 결정했다. 그중 가용성과 신뢰성, 전송능력, 경제성을 이유로 IEC 62439의 중복 네트워크 프로토콜 중 HSR(High-availability Seamless Redundancy)을 디지털 변전소를 위한 네트워크 프로토콜로 이용하기 위한 많은 연구가 진행되고 있다. 특히 디지털 변전소 내부에서도 가장 높은 수준의 가용성과 네트워크 성능을 요구하는 프로세스 버스용 네트워크 구성 프로토콜로 적합하다. 본 논문은 실제 154Kv급 변전소 프로세스 버스에 HSR을 적용하였을 때 성능을 분석하였다.

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Estimation of Ride Comfort for Korean High Speed Train at High Speed (고속에서 한국형 고속철도 차량의 승차감 추정)

  • Kim, Young-Guk;Kim, Seog-Won;Mok, Jin-Yong;Kim, Sang-Su;Kim, Ki-Hwan
    • Journal of the Korean Society for Railway
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    • v.10 no.2 s.39
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    • pp.146-152
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    • 2007
  • The ride comfort is more important in the train speedup. Generally, it is defined as the vehicle vibration. There are many studies on evaluation method of ride comfort for railway. But the ride comfort for Korean high speed train(HSR 350x) has been assessed by statistical method according to UIC 513R. It is impossible for HSR 350x to run at constant speeds above 310km/h during 5 minutes required in UIC 513R because of the same operational condition as KTX and the design limitation of infrastructures. The ride indices data had been acquired from 80 to 310km/h through the on-line test. The present study has suggested the methodology for the estimation of the ride indices at high speed above 310km/h by using those obtained below 310km/h and reviewed the ride comfort for HSR 350x in these speeds.

Torque Measurement of Tripod Shaft for HSR-350x (한국형고속열차의 동력전달축 부하측정)

  • Kim, Sang-Soo;Kim, Young-Guk;Kim, Ki-Hwan;Park, Choon-Soo
    • Journal of the Korean Society for Railway
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    • v.10 no.6
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    • pp.660-664
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    • 2007
  • Korea High-speed Railway (HSR-350x) was developed by Korean government, several institutes, and related companies. HSR-350x was investigated its performance and tested at the high speed line. In 2004 December, HSR-350x was run over 350km/h and proved its running performance. And Korean Train Express (KTX) served the commercial traffic for 4 years. These high speed railways have 12 motors to transfer the traction effort or the braking force. To obtain the load of the transfer shaft, it is necessary to measure the transferred torque of the shaft. In this paper, authors propose the construction the measurement system fur monitoring the force transferred to the train from the motor The system was installed to the tripod shaft which is located between the reduction gears and it measures the mechanical load directly.

A Study on the Speed-instructions for Increasing Speed of the Train in High Speed Railway (고속철도 증속에 따른 폐색 표준 속도 수립에 대한 연구)

  • Bang, Yung;Kim, Hyun-Min;Cho, Shin-Young;Cho, Yong-Gi
    • Proceedings of the KSR Conference
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    • 2010.06a
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    • pp.1081-1099
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    • 2010
  • The TVM 430 system manufactured by Ansaldo STS in France is currently used in ground signalling system for Kyungbu HSR. It transmits the speed information to the on-board signalling system in the form of continuous signal via the track, and the rolling stock in Kyungbu HSR runs with 300km/h max. operating speed by using the corresponding information. Looking from the recent international trends in HSR, reducing the travelling time and increasing of the line capacity is promoting via the improvement of train speed. In case of TGV Est, they are realizing the normal operation with 320km/h max. operating speed by using TVM SEI signalling system, which is similar to TVM 430. Furthermore, in case Honam HSL, which is under construction, is looking over faster speed than the limited one of Kyungbu HSR(i.e. over 300km/h). In this paper, it is assumed that the existing TVM 430 ground signalling system is used and train speed is improved, therefore the number of block section to be increased depending on the increase of train speed and the standard speed to be used in this case is drawn via the simulation of the train model and described the method accordingly.

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Examination on the variation of ride comfort for korean high speed train through on-line test (시운전 시험을 통한 한국형 고속열차의 승차감 변화추세에 관한 연구)

  • Kim, Young-Guk;Mok, Jin-Yong;Kim, Seog-Won;Park, Chan-Kyeong;Kim, Ki-Hwan;Park, Tae-Won
    • Journal of Sensor Science and Technology
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    • v.14 no.3
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    • pp.144-149
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    • 2005
  • In this paper, we introduce the variation of ride characteristics for HSR 350x(Korea High Speed Train project) through on-line test during about 3 years. The concept of term "ride comfort" is equivocal. Generally it is evaluated as the vehicle vibration. The ride comfort for HSR 350x has been assessed by statistical method according to UIC 513R. The testing results show that HSR 350x has no problems from the viewpoint of the comfort ride on the high speed line and conventional line and that 1st and 2nd suspensions play an important role in the ride characteristics for high speed train.

Shear strength evaluation of RC solid piers of high-speed railway bridges in China

  • Guo, Wei;Fan, Chao;Cui, Yao;Zeng, Chen;Jiang, Lizhong;Yu, Zhiwu
    • Structural Engineering and Mechanics
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    • v.78 no.4
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    • pp.413-423
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    • 2021
  • Piers are the main lateral force-resisting members of high-speed railway (HSR) bridges used in China and are characterized by low axial load ratios, low longitudinal reinforcement ratios, low stirrup ratios, and high shear span ratios. It is well known that flexural, flexural-shear, and shear failures of piers may occur during an earthquake. In this study, a new shear strength model was developed to simulate the seismic failure of HSR solid piers accurately. First, low cyclic-loading test data of solid piers obtained in recent years were collected to set up a database for model verification. Second, based on the test database, the applicability of existing shear strength models was evaluated. Finally, a new shear strength model for HSR solid piers with round-ended cross-sections was derived based on the truss model and ultimate equilibrium theory. In comparison with existing models, it was demonstrated that the proposed model could be used to predict the shear strength of HSR piers more accurately.

Estimation of Willingness-to-pay for Mitigating Crowdedness in High-speed Rail Trains (고속철도 차량 내 혼잡도 완화에 대한 지불 용의액 추정 연구)

  • Lee, Jang-Ho;Kim, Dong-Sun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.37 no.2
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    • pp.379-387
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    • 2017
  • As the average load factor of High-Speed Rail (HSR) trains increased up to 98%, it caused uncomfortable crowdedness in the cabin and deteriorated the level of service to the HSR users. In this paper, we estimated the willingness-to-pay (WTP) of the HSR users for mitigating the crowdedness in the cabin. The contingent valuation method based on the stated preference data was used and the tobit model was applied for the estimation of WTP. It can be concluded that WTP for mitigating the crowdedness in the HSR trains is proportional to the congestion level of train and income level of user. Males, first-class users, not-assigned ticket users, and monthly pass users have relatively higher WTPs, while homemakers, travelers in weekends, commuters and leisure travelers have relatively lower values. The results can provide the fundamental information to estimate the benefits of mitigating crowdedness or the magnitude of governmental subsidy to railway operators for additional introduction of HSR trains.

Construction and analysis of painting probe for homogeneously staining regions in human neuroblastoma cell line IMR-32

  • Park, Sun-Hwa;Kim, Ho-Chung;Chun, Yong-Hyuck
    • Journal of Genetic Medicine
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    • v.1 no.1
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    • pp.45-50
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    • 1997
  • Neuroblastoma, a pediatric malignant neoplasm of neural crest origin, has a wide range of clinical virulence. The mechanisms contributing to the development of neuroblastomas are largely unclear, but non-random chromosomal changes identified over the past years suggest the involvement of genetic alterations. Amplification of the human N-myc proto-oncogene is frequently seen either in extrachromosomal double minutes or in homogeneously staining regions (HSRs) of aggressively growing neuroblastomas. N-myc maps to chromosome 2 band 24, but HSR have never been observed at this band, suggesting transposition of N-myc during amplification. We have constructed and analyzed the region-specific painting probe for HSR in neuroblastoma IMR-32 to determine the derivative chromosomes. Microdissection was performed on HSR using an inverted microscope with the help of microglass needles and an micromanipulator. We pretreated the microdissected fragments with Topoisomerase I which catalyzes the relaxation of supercolled DNA, and performed two initial rounds of DNA synthesis with T7 DNA polymerase followed by conventional PCR to enable the reliable preparation of Fluorescent in situ hybridization probe from a single microdissected chromosome. With this method, it was possible to construct the region-specific painting probe for HSR. The probe hybridized specifically to the HSRs of IMR-32, and to 2p24, 2p13 of normal chromosome. Our results suggest there was coamplification of N-myc together with DNA of the chromosome 2p24 and 2p13. Moreover, the fluorescent signals for the amplified chromosomal regions in IMR-32 cells were also easily recognized at a Thus this painting probe can be applied to detect the similar amplification of N-myc in neuroblastoma tissue, and the probe pool for HSR may be used to identify the cancer-relevant genes.

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