• Proceedings of the Korean Society of Life Science Conference
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• 2001.06a
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• pp.67-86
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• 2001

A strain producing strongly fibrinolytic enzyme was isolated from soil and was identified to be Bacillus subtilis by biochemical and physiological characterization. The optimal culture conditions for the production of fibrinolytic enzyme was determined to be 1.0% tryptone, 1.5% soluble starch, 0.5% Peptone, 0.5% NaCl, $(NH_{4})_{3}PO_4.3H_{2}O, and MgSO_{4}.7H_{2}O.$ Initial pH and temperature were pH 8.0 and $30^{\circ}C$ , respectively, The highest enzyme production was observed at 30 hours of cultivation at $30^{\circ}C$ The fibrinolytic enzyme was purified to homogeneity by DEAE Sephadex A-50 ion exchange column chromatography, 70% ammonium sulfate precipitation, Sephadex G-200 and G-75 gel filtration column chromatography. The molecular weight of the purified enzyme was 28,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A gene encoding the fibrinolytic enzyme was cloned into a plasmid vector pBluescript, transforming E.coli XL-1 Blue. The clone was able to degrade fibrin, This indicated that the gene could encode a fibrinolytic enzyme. The nucleotide sequence of the 2.7 kb insert was determined in both direction. One open reading frame composed of 1023 nucleotides was found to be a potential protein coding region. There was the putative Shine-Dalgano sequence and TATA box upstream of the open reading frame. The homology search data in the genome database showed that both the 2.7 kb insert and 1 kb open reading frame carried no significance in the nucleotide sequence of known fibrinolytic enzyme from Bacillus serovars. The recombinant cell harboring the novel gene involved in fibrinolysis was subjected to protein purification. The molecular mass of the purified fibrinolytic enzyme was determined to be 31864 Dalton, which was highly in accordance with the molecular mass(33 kDa) of the fibrinolytic gene deduced from the insert. The fibrinolytic enzyme was Purified 50.5 folds to homogeneity in overall yield of 10.7% by DEAE Sephadex A-50 ion exchange, 85% ammonium sulfate precipitation, Sephadex G-50, Superdex 75 HR FPLC gel filtration. In conclusion, a novel fibrinolytic gene from Bacillus subtilis was identified and characterized by cloning a genomic library of Bacillus subtilis into pBleuscript. For the soybean fermented by this strain, it is found that there increased assistant protein about 20% compared to the soybean not fermented and increased about 30% according to amino acid analysis and, in particular, essential amino acid increased about 40%. When keeping this fermented soybean powder at room temperature for about 70days, it showed very high stability maintaining almost perfect activity and, therefore, it gave us great suggestion its possibility of development as a new functional food.

• The Korean Journal of Food And Nutrition
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• v.18 no.4
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• pp.309-315
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• 2005

The objective of this study was to investigate an influence of chitosan addition on quality attributes of sponge cake. The cake was made with various chitosan concentration (1,000, 2,000, and 3,000 ppm), and it was stored for 120 hr at three temperatures (5, 15, and $25^{\circ}C$). By analyzing the moxogram data of wheat flour-chitosan composite flour, the ascending angle of the samples containing chitosan was sharply increased and the descending angle had lower values compared to that of control as the chitosan addition concentration was increased. The loaf weights and volumes of the samples with chitosan were increased compared to those of the control. Crumb firmness of the control showed relatively high value compared to those ($5\~6$ N) of other samples containing chitosan regardless of storage temperature and storage period, and the firmness after 5 days storage at $5^{\circ}C$ showed the highest value (about 9.3 N). Sensory evaluation attributes of sponge cakes did not show significant differences by chitosan addition up to concentration of 2,000 ppm.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.441-441
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• 2015

북한의 수자원 현황을 분석하고 미래를 전망하는 일은 수자원 분야 전문가들의 공통된 관심사 중 하나이다. 그러나 많은 노력에도 불구하고 제한된 자료로 인해 이에 대한 체계적인 성과가 구축되지 못한 것이 사실이다(안재현 등, 2010). 북한 수자원 현황과 관련해서 1994년 한국수자원공사의"북한 수자원 현황 및 개발 동향"에 대한 연구와 1996년 한국과학기술단체총연합회의"남북한 수자원 비교평가 연구"가 수행된 바 있다. 본 연구에서는 2012년 7월 북한의 홍수피해에 대하여 북한지역의 빈약한 강우량 정보를 활용하여 위성으로부터 유도된 강우자료를 보정하여 북한지역의 홍수량을 산정하는데 활용할 수 있는 시계열 자료를 생성하는 것을 목적으로 하였다. 2012년 북한 홍수피해는 조선 중앙통신 보도에 의하면 6월 말부터 7월말까지 169명 사망, 400여명 실중, 144명 상해의 인명피해와 평안남북도, 자강도, 함경남북도 등 4만 3770여 가구가 침수되었으며, 농경지 피해는 6만 5280정보가 유실, 매몰, 침수(평안북도 2만 3400정보, 평안남도 2만 1900정보, 함경남도 5679정보, 함경북도 7220정보)의 피해를 입은 것으로 보도하고 있다(http://www.hani.co.kr/arti/politics/defense/545795.html). 한편, 북한의 기상수문국 통보에 의하면 2012년 장마가 시작된 이후 20일 동인 평균 강우량의 2배 가까운 비가 내렸으며, 특히 7월 19일 오후 9시부터 21일 오후 3시 사이 자강도 동신군에 413mm, 송원군에 383mm, 희천시에 322mm, 평안북도 태천군에 380mm, 향산군에 312mm, 동창군에 304mm의 폭우가 쏟아졌다고 조선중앙통신은 덧붙였다. 본 연구에서는 위성으로부터 유도된 강수자료 중 GSMaP_NRT자료를 활용하여 북한의 기상수문국에서 통보한 같이 기간의 강수량 자료를 분석한 결과 자강도 동신군 지역의 경우 총강우는 208.8mm로 분석되었으며, 평균강우는 9.49mm, 최대강우강도는 17.7mm/hr로 분석되었다. 두 자료의 총강우량에 대한 상대오차는 약 49.5% 정도의 오차가 있는 것으로 분석되었다. 위성강우 보정 방법으로 Immerzeel(2001) 등은 위성으로 관측된 시계열 자료와 지상계측된 시계열 자료를 분석하여 두 자료간의 평균과 표준편차를 이용하여 평균 및 표준편차 계수를 산정하여 위성자료에 대한 보정강우를 생성하는 방법을 제시한 바 있다. 본 연구에서는 위성으로부터 유도된 강우량 자료는 시계열 자료이나 지점계측 강우는 총강우만 존재하여 총강우에 대한 시계열 자료를 위성강수자료를 무차원화하여 지점계측의 위성 보정강우를 생성하였다.

• Journal of Korea Water Resources Association
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• v.54 no.spc1
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• pp.1131-1141
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• 2021

In this study, the artificial neural network model is applied for real-time dam inflow prediction and then evaluated for the prediction lead times (1, 3, 6 hr) in dam basins in Korea. For the training and testing the model, hourly precipitation and inflow are used as input data according to average annual inflow. The results show that the model performance for up to 6 hour is acceptable because the NSE is 0.57 to 0.79 or higher. Totally, the predictive performance of the model in dry seasons is weaker than the performance in wet seasons, and this difference in performance increases in the larger basin. For the 6 hour prediction lead time, the model performance changes as the sequence length increases. These changes are significant for the dry season with increasing sequence length compared to the wet season. Also, with increasing the sequence length, the prediction performance of the model improved during the dry season. Comparison of observed and predicted hydrographs for flood events showed that although the shape of the prediction hydrograph is similar to the observed hydrograph, the peak flow tends to be underestimated and the peak time is delayed depending on the prediction lead time.

• Journal of Life Science
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• v.28 no.11
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• pp.1321-1331
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• 2018

The aim of this study was to separate the photosensitizer that induces apoptosis of U937 and SK-HEP-1 cells from Nostoc commune. Dried N. commune was extracted with $CH_2Cl_2/MeOH$ (1:1) to separate the photosensitizer using various chromatographic techniques. The isolated compound was identified as pheophytin a ($C_{55}H_{74}N_4O_5$) with a molecular weight of 870. Its photodynamic activities were assessed under different irradiation conditions (light and non-light) at the same concentration range of $1.15-23.0{\mu}M$. The apoptosis inducing activity in U937 or SK-HEP-1 cells appeared only in the light. The mechanisms underlying the pheophytin a-mediated photodynamic inhibition of cancer cells were further investigated by examining cell morphology changes, cytotoxicity, caspase-3/7 activity, fluorescence staining, flow cytometry analysis, and DNA fragmentation in these two cell lines. The positive control and the light irradiation group showed typical apoptotic responses, including morphological changes, cytotoxicity, caspase activity, nucleus shrinkage owing to chromatin condensation, DNA laddering, and the presence of apoptotic bodies. Cytotoxicity markedly increased in a dose-dependent manner after a 12 hr exposure. Caspase-3/7 activity was higher in U937 cells than in SK-HEP-1 cells. Apoptosis induction therefore appeared to be both concentration- and light-dependent. In conclusion, pheophytin a, isolated from the blue green alga N. commune, had a photodynamic apoptosis-inducing effect on U937 and SK-HEP-1 cells. The findings reported here can be used as basic data for the development of next-generation photosensitizers from N. commune.

• Journal of Digital Convergence
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• v.17 no.8
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• pp.283-291
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• 2019

The aim of investigated the role of FABP5 in the hepatic lipogenesis and lipid metabolisms. Mice were overexpressed and silenced liver FABP5 using virus particles. Mice were fed a Western-type diet or regular chow for 1week and then sacrificed mouse after 24hr fasted. Liver homogenates were used for protein analysis by Western blot and mRNA levels by RT-PCR. Hepatic and serum lipids were analysed by thin-layer chromatography. Mice fed a Western-type or high saturated fat diet revealed large increases in FABP5 expression. However, FABP5 mRNA levels were drastically reduced under fasted. Hepatic TG was significantly increased FABP5-OEAV mice, but a significantly decreased hepatic free cholesterol under fed. The discovered a substantial decrease in hepatic TG mass with FABP5 silencing. In these data, presented evidence for an important role of FABP5 in hepatic lipogenesis and hepatic TG storage. FABP5 may also be a potential target in the treatment of NAFLD, metabolic syndrome, and obesity. Furthermore, studies to which transcription factors are involved in FABP5 expression and regulation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.159-170
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• 2021

As the main purpose of R&D changes from the center of knowledge creation to the center of economic value creation through technology transfer and commercialization, public technologies can also secure economic feasibility as well as make a social contribution. Korea has been focusing on fostering core human resources who can lead the commercialization of basic and original research results by launching the 'Support project for exploring startups linked with public technology-based markets' since 2015 in order to promote public technology startup. This study is based on the results of a survey for the purpose of analyzing the performance of this project. In addition, this study derived four factors related to the I-corps project performance from the results of this survey and verified the relationship between these factors through structural equation model analysis. In summary, it was confirmed that 'Application Level' and 'Business Model,' which are positively affected directly from 'Entrepreneurship Learning,' have positive effects on 'Financial Resources'. Furthermore, the indirect effect of 'Entrepreneurship Learning' on 'Financial Resources' was verified. In particular, the high level of impact of 'Entrepreneurship Learning' on 'Application Level,' and the impact of 'Application Level' on 'Business Model' and 'Financial Resources' were also positive.

• Atmosphere
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• v.33 no.4
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• pp.367-385
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• 2023

The variabilities of precipitation and particulate matters (i.e., PM₁₀ and PM_2.5) and the scavenging efficiency of PMs by precipitation were quantified using long-term measurements in Seoul, Korea. The 21 years (2001~2021) measurements of precipitation and PM₁₀ mass concentrations, and the 7 years (2015~2021) of PM_2.5 mass concentrations were used. Statistical analysis was performed for each period (i.e., year, season, and month) to identify the long-term variabilities of PMs and precipitation. PM₁₀ and PM_2.5 decreased annually and the decreasing rate of PM₁₀ was greater than PM_2.5. The precipitation intensity did not show notable variation, whereas the annual precipitation amount showed a decreasing trend. The summer precipitation amount contributed 61.10% to the annual precipitation amount. The scavenging efficiency by precipitation was analyzed based on precipitation events separated by 2-hour time intervals between hourly precipitation data for 7 years. The scavenging efficiencies of PM₁₀ and PM_2.5 were quantified as a function of precipitation characteristics (i.e., precipitation intensity, amount, and duration). The calculated average scavenging efficiency of PM₁₀ (PM_2.5) was 39.59% (35.51%). PM₁₀ and PM_2.5 were not always simultaneously scavenged due to precipitation events. Precipitation events that simultaneously scavenged PM₁₀ and PM_2.5 contributed 42.24% of all events, with average scavenging efficiency of 42.93% and 43.39%. The precipitation characteristics (i.e., precipitation intensity, precipitation amount, and precipitation duration) quantified in these events were 2.42 mm hr^-1, 15.44 mm, and 5.51 hours. This result corresponds to 145% (349%; 224%) of precipitation intensity (amount; duration) for the precipitation events that do not simultaneously scavenge PM₁₀ and PM_2.5.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.5B
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• pp.485-493
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• 2008

The Artificial Neural Network (ANN) model was suggested for predicting probability of precipitation (PoP) using RDAPS NWP model, observation at AWS and upper-air sounding station. The prediction work was implemented for flood season and the data period is the July, August of 2001 and June of 2002. Neural network input variables (predictors) were composed of geopotential height 500/750/1000 hPa, atmospheric thickness 500-1000 hPa, X & Y-component of wind at 500 hPa, X & Y-component of wind at 750 hPa, wind speed at surface, temperature at 500/750 hPa/surface, mean sea level pressure, 3-hr accumulated precipitation, occurrence of observed precipitation, precipitation accumulated in 6 & 12 hrs previous to RDAPS run, precipitation occurrence in 6 & 12 hrs previous to RDAPS run, relative humidity measured 0 & 12 hrs before RDAPS run, precipitable water measured 0 & 12 hrs before RDAPS run, precipitable water difference in 12 hrs previous to RDAPS run. The suggested ANN has a 3-layer perceptron (multi layer perceptron; MLP) and back-propagation learning algorithm. The result shows that there were 6.8% increase in Hit rate (H), especially 99.2% and 148.1% increase in Threat Score (TS) and Probability of Detection (POD). It illustrates that the suggested ANN model can be a useful tool for predicting rainfall event prediction. The Kuipers Skill Score (KSS) was increased 92.8%, which the ANN model improves the rainfall occurrence prediction over RDAPS.

• Journal of the Korean Society of Food Culture
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• v.22 no.6
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• pp.833-840
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• 2007

The purpose of this study was to obtain baseline data of nutritional management for women's bone health according to age. This study was conducted through questionnaire, bone mineral density(BMD) measurement by DEXA and dietary survey by 24hr recalls to 134 adult women. Subjects were divided into three groups: young women group who aged 20-29(n=48), middle aged women group who aged over 30 and were pre-menopause(n=36), postmenopausal women group(n=48). The body fat percent of postmenopausal women group was the highest among the three groups. BMD of lumbar spine(L1-L2) were $0.93\;g/cm^2$ for young women, $0.97\;g/cm^2$ for middle aged women, and $0.88\;g/cm^2$ for postmenopausal women, respectively(p<0.05). BMD of femoral neck(p<0.05), trochanter(p<0.05), and ward's (p<0.001) were significantly decreased as age increasement. Drinking alcohol and skipping meals were significantly higher in young women groups than in the other groups, however frequency of doing regular exercise was significantly lower in young women groups. Young women group consumed the significantly higher amount of cereals(p<0.05), sugar and sweeteners(p<0.001), meats(p<0.01), eggs(p<0.01), milks(p<0.05) and oils(p<0.001) than middle aged and postmenopausal women. Also, energy(p<0.001), animal protein, (p<0.01), plant oil(p<0.001), animal fat(p<0.001), retinol(p<0.001), vitamin E(p<0.01), and cholesterol(p<0.001) intakes of young women were highest among the three groups. However, young women group consumed the significantly lower amount of dietary fiber(p<0.05), vitamin C(p<0.01), folate(p<0.05) and fruits(p<0.01) than the other groups. To summarized the our results, young women who were in still undergoing bone formation activity to develope peak bone mass, had more dietary habit and lifestyle problems than middle aged and postmenopausal women. The results of this study revealed that nutritional management and education for bone health should be emphasized in young women not only aged women.