• Title/Summary/Keyword: HPV

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Immortalization of Rat Kidney Glomerular Mesangial Cell and Its Coculture with Glomerular Epithelial Cell

  • Toshinobu Kida;Sachi Fujishima;Masatoshi Matsumra;Wang, Pi-Chao
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.2
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    • pp.92-98
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    • 2000
  • Mesangial cell has several key roles in thee control of glomerular function: it partocipates in the regulation of glomerular filtration rate, macromolecular clearance, and as both a source and target of numerous hormones and autocrines. Many of these insights into mesangial cell function have been obtained by studying mesangial cells in culture. However, no suitble cell lines have established yet. We here reported the immortalization of rat kidney glomeruar mesangial cell by transfection of E6 and E7 genes of human papillomavirus type 16 (HPV-16) via electroporation and lipofection. The reslts showed that only electroporation could transfect the genes to mesangial cells and the transfected cells maintained the viability for longer than 6 months. Fluorescence microscopic observation showed that cellular contractility and phagocytosis, which are the two main phenotypes of mesangial cells with rat glomerular epithelial cells showed that the growth of mesangial dells was suppressed by epithelial cell, but the growth of epithelisl cells was enhanced by mesangial cells. Moreover, Such results may imply that the glomerular cell-cell interaction plays an important role in the regulation of cell proliferation and differentiation.

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Automatic Reading System for On-off Type DNA Chip

  • Ryu, Mun-Ho;Kim, Jong-Dae;Kim, Jong-Won
    • Journal of Information Processing Systems
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    • v.2 no.3 s.4
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    • pp.189-193
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    • 2006
  • In this study we propose an automatic reading system for diagnostic DNA chips. We define a general specification for an automatic reading system and propose a possible implementation method. The proposed system performs the whole reading process automatically without any user intervention, covering image acquisition, image analysis, and report generation. We applied the system for the automatic report generation of a commercialized DNA chip for cervical cancer detection. The fluorescence image of the hybridization result was acquired with a $GenePix^{TM}$ scanner using its library running in HTML pages. The processing of the acquired image and the report generation were executed by a component object module programmed with Microsoft Visual C++ 6.0. To generate the report document, we made an HWP 2002 document template with marker strings that were supposed to be searched and replaced with the corresponding information such as patient information and diagnosis results. The proposed system generates the report document by reading the template and changing the marker strings with the resultant contents. The system is expected to facilitate the usage of a diagnostic DNA chip for mass screening by the automation of a conventional manual reading process, shortening its processing time, and quantifying the reading criteria.

Lactic Acid Bacteria as Oral Antigen Protein Carriers (유산균을 이용한 겸구용 항원 단백질 수송능 연구)

  • Cho, Hee-Jeong;Choi, Han-Gon;Kim, Jung-Ae;Oh, Yu-Kyoung
    • Journal of Pharmaceutical Investigation
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    • v.35 no.2
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    • pp.75-80
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    • 2005
  • A promising application of Lactococcus lactis is its use as live vehicles for production and delivery of heterologous proteins of vaccines and therapeutic substances. Because L. lactis has GRAS ('generally regarded as safe') status, we tested whether L. lactis could function as the carrier of the Ll protein of human papillomavirus (HPV) type 16. The RNA level expression of Ll gene was detected in L. Lactis. The Ll protein was expressed in L. lactis with Ll gene. The growth of strains L. lactis with an empty plasmid (pAMJ328) and L. lactis with Ll-encoding plasmid (pAMJ328-Ll) was slightly decreased in comparison with the growth of strains L. lactis (wild type). However, all the three strains of L. lactis maintained the ability to ferment sugars primarily into lactic acid, indicating that Ll protein did not affect the biochemical property of L. lactis. These results suggest that L. lactis, capable of carrying Ll protein, might be further developed as a biocompatible oral protein delivery system.

Differencies in Risk Factors for Cervical Dysplasia with the Applied Diagnostic Method in Serbia

  • Antic, Ljiljana Gojko;Vukovic, Dejana Srboljub;Vasiljevic, Mladenko Drago;Antic, Dragan Zivojin;Aleksopulos, Hristos Georgios
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.16
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    • pp.6697-6701
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    • 2014
  • Background: In the etiology of cervical cancer not only HPV infection is important, but also other factors such as demographic influences andsexual and reproductive health attitudes, as well as others related to preventive measure usage (or non usage). The aim of this study was to examine factors associated with cervical dysplasia in asymptomatic women who were examined by routine cytology and cervical biopsy for early detection of cervical cancer. Materials and Methods: Socio-demographic and other characteristics were obtained from medical files of 85 examinees with pathologic cytologic findings (Pap test) and histopathologic (HP) findings after biopsy. Results: ccording to the Pap test result, a greater probability for development of cervical dysplasia was noted with examinees having a larger number of sexual partners (OR= 5.01, 95% CI 1.04-24.10), and those who are afraid of the Pap finding. Risk factors for development of cervical dysplasia according to the bioptic finding were early beginning with sexual activities, presence of any STD in personal medical history and fear of the Pap test finding. Conclusions: The only risk factor found to be important for both methods was fear of the Pap testing finding.

Development and Application of Artificial Skin Using Tissue Engineering (조직배양공학을 이용한 인공피부의 개발 및 응용)

  • Yang, Eun-Kyung;Park, Sue-Nie;Park, Jung-Keug
    • Proceedings of the KOSOMBE Conference
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    • v.1995 no.11
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    • pp.14-17
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    • 1995
  • An in vitro construct of three dimensional artificial skin equivalent has been engineered using human cervical epithelial cells and human foreskin fibroblasts with a matrix of bovine type I collagen. Two cell lines were established from cervical uteri cancer tissues which have the HPV(human papillomavirus)18 genome. These two cell lines came from the same origin but have slight differencies in growth rate and tumorigenicity. The organotypic raft culturing of epithelial cells were accomplished at air-liquid interface. The differentiation related characteristics were examined by immunohistochemistry using monoclonal antibodies against EGFreceptor, cytokeratin 5/6/18 as proliferation markers and against filaggrin, involucrin, and cytokeratin 10/13 as differentiation marker. We have obtained the stratification and the differentiation in the artificial skin equivalent, and differentiation-related proteins were expressed more in the C3-artificial skin, and proteins of proliferation were expressed more in the C3N-artificial skin, relatively. We found that reconstituted artificial skin have the same characteristics of differentiation proteins of original tissue or cells of human body.

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Evidence-based Screening, Early Diagnosis and Treatment Strategy of Cervical Cancer for National Policy in Low-resource countries: Example of India

  • Saxena, Upma;Sauvaget, Catherine;Sankaranarayanan, Rengaswamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1699-1703
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    • 2012
  • Cervical cancer remains the most frequent cancer in women from the developing world. More than 88% of deaths occur in low-income countries, and it is predicted to climb to 91.5% by 2030. Although Pap-based screening programmes have shown to be effective in reducing the disease burden in high-resource countries, implementation and sustention of cytology-based programmes is quite challenging in low-resource settings. The present paper reviews evidence-based alternatives of screening methods, triaging algorithm, treatment of cervical precancerous lesions, and age-group at screening appropriate for low-income countries. Evidence shows that visual inspection methods using diluted acid acetic or Lugol's iodine, and HPV-DNA testing are more sensitive tests than the Pap-smear screening test. Visual inspection allows an immediate result and, when appropriate, may be immediately followed by cryotherapy, the so called "screen-and-treat" approach, achieved in a single visit, by trained nurses and midwives. Examples of cervical cancer prevention programmes in India and selected low-income countries are given.

Rigid Bronchoscopic Treatment for an Adult Case of Multiple Squamous Papillomatosis in the Trachea (성인에서 발생한 다발성 기관 유두종증에 대한 경직성 기관지경을 이용한 치험 1 예)

  • Jung, Bock-Hyun;Lim, Jae-Min;Kim, Mi-Hye
    • Korean Journal of Bronchoesophagology
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    • v.15 no.2
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    • pp.81-86
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    • 2009
  • A 57 year old human immune virus(HIV)-positive male presented with a progressive dyspnea for 6 months. Chest CT showed multiple polypoid masses arising from upper tracheal wall. Bronchoscopic examination revealed that multiple large cauliflower-like polypoid tumors was obstructing tracheal lumen. They were diagnosed as multiple squamous papillomas and were removed by Nd:YAG laser photocoagulation and rigid bronchoscopic treatment. The tumors were histologically diagnosed as squamous papilloma infected with human papilloma virus(HPV) type 6 and 11 in in-situ hybridization. Rigid bronchoscopy might be safer and more efficient than flexible bronchoscopy for the treatment of multiple tracheal papillomatosis obstructing tracheal lumen because of easy establishment of airway patency and direct use of rigid bronchoscope itself for tumor resection.

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Properties of Starches in Chinese Yam, Dioscorea oppsita Thunb. Irradiated with Proton Beam

  • Kim, Sang-Kuk;Choi, Hong-Jib;Kim, Kye-Ryung;Kim, Hak-Yoon
    • Korean Journal of Plant Resources
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    • v.24 no.3
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    • pp.304-308
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    • 2011
  • The survival rate and thermal, crystal, and physicochemical properties of starches in chinese yam tubers irradiated to proton beam were determined. Survival rate was decreased with increased proton beam irradiation. Amylose content of D. opposita starches from different proton beam ranged from 13.2% to 17.8%. D. opposita starch at 5 Gy showed the highest ${\Delta}H_{gel}$ values (12.0 J/g) while D. opposita starch at 25 Gy showed the lowest values (10.1 J/g). Several parameters such as PKV (peak viscosity), HPV (Hot peak viscosity) and CPV (Cooling peak viscosity) decreased with the increase in irradiation dose. The degrees of crystallinity of the D. opposita starches at 5, 10, 15, 20 and 25 Gy were 37.2, 38.3, 38.9, 39.2 and 39.9%, respectively. It might be deduced that proton beam irradiation causes changes of starch, especially at high dose irradiation.

Incidence and Mortality from Mucosal Head and Neck Cancers amongst Australian States and Territories: What It Means for the Northern Territory

  • Singh, Jagtar;Jayaraj, Rama;Baxi, Siddhartha;Ramamoorthi, Ramya;Thomas, Mahiban
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.5621-5624
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    • 2013
  • Mucosal head and neck cancers are squamous cell carcinomas that develop in the upper-aero digestive epithelium. Together they constitute the sixth most common cancer with an estimated 900,000 new cases and 350,000 deaths each year reported worldwide. The risk factors are tobacco, alcohol and human papillomavirus (HPV). Our research team initially reported a high incidence rate of HNC in the indigenous population of the Northern Territory. Mortality rates also vary in the Australian States and Territories, with particularly high mortality observed in the Northern Territory. There is a paucity of incidence studies of HNC for the Australian States and Territories. Therefore this review primarily focuses on variation in incidence and mortality iacross the country and highlights specifically the high incidence and mortality in the Northern Territory. Attention is also given to sex-specific incidence and mortality rates.

Autophagy in Cervical Cancer: An Emerging Therapeutic Target

  • Pandey, Saumya;Chandravati, Chandravati
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.10
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    • pp.4867-4871
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    • 2012
  • Cervical cancer is a leading cause of morbidity and mortality in women worldwide. Although the human papillomavirus (HPV) is considered the major causative agent of cervical cancer, yet the viral infection alone is not sufficient for cancer progression. The etiopathogenesis of cervical cancer is indeed complex; a precise understanding of the complex cellular/molecular mechanisms underlying the initiation, progression and/or prevention of the uterine cervix is therefore essential. Autophagy is emerging as an important biological mechanism in targeting human cancers, including cervical cancer. Furthermore, autophagy, a process of cytoplasm and cellular organelle degradation in lysosomes, has been implicated in homeostasis. Autophagic flux may vary depending on the cell/tissue type, thereby altering cell fate under stress conditions leading to cell survival and/or cell death. Autophagy may in turn govern tumor metastasis and subsequent carcinogenesis. Inflammation is a known hallmark of cancer. Vascular insufficiency in tumors, including cervical tissue, leads to depletion of glucose and/or oxygen perturbing the osmotic mileu causing extracellular acidosis in the tumor microenvironment that may eventually result in autophagy. Thus, targeted manipulation of complex autophagic signaling may prove to be an innovative strategy in identification of clinically relevant biomarkers in cervical cancer in the near future.