• Journal of Microbiology and Biotechnology
• /
• v.15 no.4
• /
• pp.844-854
• /
• 2005

We constructed a defined physical and genetic map of H. pylori strain 51, previously isolated from a Korean patient with a duodenal ulcer, by combining a restriction analysis by pulse-field gel electrophoresis with the construction of a BAC library. A Notl-digest of H. pylori strain 51 genome yielded seven fragments, from which the genomic size was estimated to be 1,698$\pm$24 kb. The BAC library was constructed from 50 to 200 kb fragments of HindIII-digested genomic DNA. From 700 BAC clones, an ordered overlapping maxi-set of 82 BAC clones was assembled that covered the entire genome. The positions of 15 genes were localized in the strain 51 genome with 4-22 kb of resolution and were compared with their orthologues in strain 26695 and strain J99. The arrangement of the 15 genes was identical in strain 51 and strain J99, except for flaA and hpaA. The plasticity zone of strain 51, like that of strain J99, was located in the single region, and was shorter than those of strain 26695 and strain J99. The strain 51 plasticity zone consisted of ORFs common only to strain 51 and J99 or to strain 51 and 26695, as well as strain 51-specific ORFs. Three genetic translocations and/or inversions were found between orthologue ORFs in strain 51 and strain J99. These results show that the chromosomal organization of strain 51 differs from Western strains such as strain 26695 and strain J99.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.40 no.4
• /
• pp.413-421
• /
• 2014

Corticotropin-releasing factor (CRF) is involved in the stress response and there is increasing evidence that stress influences skin disease such as hair loss. In cultured human hair follicles, CRF inhibits hair shaft elongation, induces premature regression and promotes the apoptosis of hair matrix keratinocytes. We investigated whether CRF influences the dermal papilla cells (DPC) that play pivotal roles in hair growth and cycling. Human DPCs were treated with CRF, adrenocorticotropic hormone (ACTH) and cortisol, key stress hormones along the hypothalamic-pituitary -adrenal (HPA) axis for 1-24 h. Interestingly, CRF modulated the expression of cytokines related to hair growth (KGF, Wnt5a, $TGF{\beta}-2$, Nexin) and increased cAMP production in cultured DPCs. CRF receptors were down-regulated by negative feedback systems. Pretreatment of CRF receptor antagonists or protein kinase A (PKA) inhibitor prevented the CRF-induced modulation. Since the CRF induces proopiomelanocortin (POMC) expression through the cAMP/PKA pathway, we analyzed POMC mRNA. CRF stimulated POMC expression in cultured human DPCs, yet we were unable to detect ACTH levels by western blot. These results indicate that CRF operates within DPCs through CRF receptors along the classical CRF signaling pathway and CRF receptor antagonists could serve as potential therapeutic and cosmetic agents for stress-induced hair loss.

• Journal of the Korean earth science society
• /
• v.34 no.7
• /
• pp.645-661
• /
• 2013

The synoptic, thermodynamic, and dynamic characteristics of a heavy snowfall event that occurred in Seoul metropolitan area on 27 to 28 December 2010 was investigated. During this period there was a distinctive case that was identified as a polar low. We analyzed surface and upper level weather charts, snowfall amount, sea surface temperature, satellite imagery, sounding, and the National Center for Environmental Prediction global $1^{\circ}{\times}1^{\circ}$ reanalysis data. The polar low developed in an area where there was strong baroclinicity in the lower level aided by strong conditional instability due to 925 hPa warm air advection and 700 hPa cold air advection. The development mechanism of polar low is due, in part, to the tropopause folding, which advected stratospheric air increasing potential vorticity in mid-level and inducing cyclonic vorticity and convergence in low-level. Eventually clouds developed and there were snowfall total of 10 cm in Seoul metropolitan area and as much as 20 cm in southern parts of Korea. During the snowfall development, there was a $-45^{\circ}C$ cold core at 500 hPa and shortwave maintained $3-5^{\circ}$ separation with surface trough, which favored the development of polar low located in the warm sector and cyclonic advection area. The height of the dynamical tropopause lowered to 700 hPa during the peak development and increase in potential vorticity allowed strong vertical motion to occur. Overall, there was a close relationship between the development of snowfall and tropopause undulation. The heaviest snowfall occurred east of the tropopause folding where strong cyclonic vorticity, vertical motion, and moisture advection all coincided while the polar low was passing through the Korean peninsula.

• Journal of Yeungnam Medical Science
• /
• v.11 no.1
• /
• pp.63-71
• /
• 1994

The purpose of this study was to evaluate the effect of stroke on hypothalamic-pituitary axis using dexamethasone suppression test. The effects were evaluated according to age, sex, type, size, and lesion site of stroke. These tests were performed in 62 patients with stroke(cerebral infarction, 42 cases : intracerebral hemorrage, 20 cases) and 21 disabled controlled patients without intracranial diseases at Yeungnam University Hospital from June 1992 to June 1993. The results summarized as follows. 1. Cerebral infarction showed significantly higher frequency of DST non-suppression in stroke patients than control(p<0.05). 2. Patients with left hemisphere stroke showed more frequent abnormal neuroendocrine test results(p<0.01) 3. Patients with large infarction revealed strongly non-suppressed DST results(p<0.01). 4. Sinificantly higher basal cortisol level in patients with cerebral infarction was noted(p<0.01). 5. There are no statistical significance between DST results and sex, age, motor impairment, type of cerebral infarction.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.34 no.11C
• /
• pp.1098-1105
• /
• 2009

In this paper, we propose an asynchronous UWB (Ultra Wide Band) Positioning scheme that can provide precise positioning performance with low complexity and low power consumption. We also present the residual test to improve the positioning performance in multipath channels having heavy NLoS (Non-Line of Sight) components. As compared to conventional ToA (Time of Arrival) positioning scheme that requires round-trip transmissions as many as the number of beacons, the proposed UWB positioning scheme effectively decrease power consumption and processing delay since a single round-trip transmission is only required. Also, as compared to conventional TDoA (Time Difference of Arrival) positioning scheme requiring precise synchronization among the beacons, asynchronous nature of the proposed scheme achieves very low system complexity. Through simulations in LoS (Line of Sight) channel models, we observe that the proposed scheme requires low system complexity, low power consumption, while providing positioning performance of almost the same accuracy as the conventional ToA and TDoA positioning schemes. In addition, the proposed scheme by employing the residual test achieves accurate positioning performance even in multipath channels having heavy NLoS components.

• BMB Reports
• /
• v.28 no.6
• /
• pp.527-532
• /
• 1995

Human taurine transporter has 12 transmembrane domains and its molecular weight is 69.6 kDa. The long cytoplasmic carboxy and amino termini might function as regulatory attachment sites for other proteins. Six potential protein kinase C phosphorylation sites have been reported in human taurine transporter. In this report, we studied the effects of phorbol 12-myristate 13-acetate (PMA) and glucocorticoid hormone on taurine transportation in the RAW 264.7, mouse macrophage cell line. When the cells were incubated with $[^{3}H]taurine$ in the presence or absence of $Na^+$ ion for 40 min at $37^{\circ}C$, the [$[^{3}H]taurine$ uptake rate was 780-times higher in the $Na^{+}-containing$ buffer than in the $Na^{+}-deficient$ buffer, indicating that this cell line expresses taurine transporter protein on the cell surface. THP1, a human promonocyte cell line, also showed a similar property. The $[^{3}H]taurine$ uptake rate was not influenced by the inflammatory inducing cytokines such as interleukin-1, gamma-interferon or interleukin-1+gamma-interferon, but was decreased by the PMA in the RAW 264.7 cell line. This suggests that activation of protein kinase C inhibits taurine transporter activity directly or indirectly. The inhibition of $[^{3}H]taurine$ uptake by PMA was time-dependent. Maximal inhibition occurred in one hr stimulation with PMA Increasing the treatment time beyond one h reduced the $[^{3}H]taurine$ uptake inhibition due to the depletion or inactivation of protein kinase C. The cell line also showed concentration-dependent $[^{3}H]taurine$ uptake under PMA stimulation. The phorbol-ester caused 23% inhibition at the concentration of 1 ${\mu}m$ PMA. The inhibition was significant even at a concentration as low as 10 nM PMA The reduced $[^{3}H]taurine$ uptake could be recovered by treatment with glucocorticosteroid hormone. Dexamethasone led to recover of the reduced taurine uptake induced by phorbol-ester, recovering maximally after one hr. This may suggest that macrophage cells require higher taurine concentration in a stressed state, for the secretion of glucocorticoid hormone is increased by hypothalamo-pituitary-adrenocortical (HPA) axis activation in the blood stream.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.31 no.4
• /
• pp.183-190
• /
• 2018

This paper presents optimization of the main spar of Human-Powered Aircraft (HPA) wing. Mass minimization was attempted, while considering large torsional deformation of the beam. Sequential Quadratic Programming (SQP) method was adopted as a relevant tool to conduct structural optimization algorithm. An inner diameter and ply thicknesses of the main spar were selected as the design variables. The objective function includes factors such as mass minimization, constant tip bending displacement, and constant tip twist of the beam. For estimation of bending and torsional deformation, the geometrically exact beam model, which is appropriate for large deflection, was adopted. Properties of the cross sectional area which the geometrically exact beam model requires were obtained by Variational Asymptotic Beam Sectional Analysis (VABS), which is a cross sectional analysis program. As a result, maintaining tip bending displacement and tip twist within 1.45%, optimal design that accomplished 7.88% of the mass reduction was acquired. By the stress and strain recovery, structural integrity of the optimal design and validity of the present optimization procedure were authenticated.

• Korean Journal of Remote Sensing
• /
• v.12 no.2
• /
• pp.126-138
• /
• 1996

The characteristics of rapid development of the low pressure system over the East Sea from 06 to 08 Nov., 1995 has been analyzed in detail by the synoptic numerical products and satellite observations. The Low system was initially triggered the development of the baroclinic leaf cloud over the border of the northern part of Korea and China and moved eastward and then developed explosively com-ma or lambda type cloud system over the East Sea. To forecast well the general development and movement of the coastal winter cyclone over the East Sea popularly in a numerical simulation by several scientists, the large baroclinicity, continuous support of water vapor, and sequential cold outbreak over the warm sea surface have been more commonly concerned about. The cyclone which the central surface pressure was dropped 40hPa within 24 hours has often accompanied strong wind and heavy snow- or rain-fall in the winter season. In all successive observations with 12-hourly satellite imagery and analyzed meteorological variables in this period, the centers of the sea-level pressure and 500hPa geopotential height associated with this cyclone were typically illustrated by moving farther eastward using GMS combined enhanced IR images. The maxi-mum wind sustained by this system with the intensity and central pressure of tropical storm was about 60 knots with the center pressure drop of 44hPa/day similar to the North American cyclonic bomb and Atlantic storm.

• Korean Journal of Poultry Science
• /
• v.30 no.4
• /
• pp.289-299
• /
• 2003

Effects of various combinations of corn oil (CO) and perilla oil (PO) as respective dietary sources of ${\omega}$-6 and ${\omega}$-3 polyunsaturated fatty acids on fatty acid profiles of immune organs were studied in young chicks. Seventy-five 1-day-old male (ISA Brown) chicks were assigned to five treatments with three replications. Semi-purified-type diets containing glucose and soybean meal as major ingredients were added with 8% CO, 6% CO+2% PO, 4% CO+4% PO, 2% CO+6% PO and 8% PO and fed for 7 weeks. There were no significant differences in body weight gain, feed intake and relative weights of liver and immune organs (g/100g weight) among dietary groups. Dietary fatty acid patterns were generally reflected in the fatty acid compositions of all immune organs such as spleen, thymus and bursa of Fabricius. The levels of a-linolenic acid(LNA), eicosapentaenoic acid (EPA) and docosahexaenoic acid in various immune organs increased with increasing levels of perilla oil in the diets, whilet the levels of linoleic acid (LA) and arachidonic acid (AA) decreased. Thymus appeared to have capacity to retain remarkably higher (P<0.05) levels of LA and LNA up to 37 and 22%, respectively, compared to the other organs. Thymic tissue contained ${\omega}$-3 fatty acid and ${\omega}$-6 fatty acid 10~36 times and 3~5 times higher than the other organs, respectively. Spleen tissue was specifically higher (P<0.05) in the levels of AA and EPA and the ratios of AA/LA and EPA/LNA, compared to the other organs, suggesting that the tissue might have high desaturase activity to convert LA or LNA to AA or EPA, respectively. BSA antibody production tended to increase by 18 ~ 32% with higher levels of perilla oil in diet, although the increase was not statistically significant. In conclusion, fatty acid compositions of immune organs very depending on the lipid composition of the diets and each organ appears to respond differently for its fatty acid profile to dietary lipids. Considering AA and EPA are precursors of many important eicosanoids, further studies are required to clarify the responses of the immune organs to the dietary fatty acids.

• Clean Technology
• /
• v.25 no.1
• /
• pp.91-97
• /
• 2019

Oxidative desulfurization (ODS) has received much attention in recent years because refractory sulfur compounds such as dibenzothiophenes can be oxidized selectively to their corresponding sulfoxides and sulfones, and these products can be removed by extraction and adsorption. In this work, The oxidative desulfurization of marine diesel fuel was performed in a batch reactor with hydrogen peroxide ($H_2O_2$) in the presence of various supported heteropoly acid catalysts. The catalysts were characterized by XRD, XRF, XPS and nitrogen adsorption isotherm techniques. Based on the sulfur removal efficiency of promising silica supported heteropoly acid catalysts, the ranking of catalytic activity was: $30\;H_3PW_{12}/SiO_2$ > $30\;H_3PMo_{12}/SiO_2$ > $30\;H_4SiW_{12}/SiO_2$, which appears to be related with their intrinsic acid strength. The $30\;H_3PW_{12}/SiO_2$ catalyst showed the highest initial sulfur removal efficiency of about 66% under reaction conditions of $30^{\circ}C$, $0.025g\;mL^{-1}$ (cat./oil), 1 h reaction time. However, through the recycle test of the $H_3PW_{12}/SiO_2$ catalyst, significant deactivation was observed, which was attributed to the elution of the active component $H_3PW_{12}$. By introducing cesium cation ($Cs^+$) into the $H_3PW_{12}/SiO_2$ catalyst, the stability of the catalyst was improved with changing the solubility, and the $Cs^+$ ion exchanged catalyst could be recycled for at least five times without severe elution.