• Title/Summary/Keyword: HP20

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Comparison of the Exopeptidase Activity of Fractions from Crude Extracts of Octopus Octopus vulgaris Cuvier Hepatopancreas Using Different Fractionation Methods

  • Kim, Min Ji;Kim, Hyeon Jeong;Kim, Ki Hyun;Heu, Min Soo;Kim, Jin-Soo
    • Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.181-187
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    • 2014
  • This study was performed to identify the optimum fractionation method and conditions to obtain exopeptidase-active fractions from octopus hepatopancreas (HP) crude extracts (CEs) using four techniques: solid ammonium sulfate fractionation, polyethylene glycol (PEG) fractionation, anion exchange chromatography, and gel filtration chromatography. The fractions with the highest total activity toward L-leucine-p-nitroanilide (Leu-pNA) were fraction IV from the ammonium sulfate and PEG fractionation, and fraction II in ion exchange and gel filtration chromatography. The total exoprotease activity of these fractions was highest in fraction IV (4,050.20 U) of ammonium sulfate fractionation, followed by fraction II (3,600.28 U) from gel filtration chromatography, fraction IV (2,861.30 U) from PEG fractionation, and fraction II (2,576.28 U) from ion exchange chromatography. These results suggest that ammonium sulfate fractionation using 60-80% ammonium sulfate was the most efficient method for separating the exoprotease active fractions from CEs of octopus HP.

An Experimental Study on Performance Characteristics of Two-Stage Compression Refrigeration Systems (2단압축 냉동장치의 성능특성에 관한 실험적 연구)

  • 김재돌;오후규;김성규;권옥배
    • Journal of Advanced Marine Engineering and Technology
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    • v.18 no.2
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    • pp.25-32
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    • 1994
  • The characteristics of the R-22 two-stage compression refrigeration systems were investigated. The apparatus consisted of 0.5HP and 1HP hermetic reciprocating compressors for the high and low stage sides respectively, a condenser, an evaporator, a heat exchanger, four expansion valves, and two intercoolers. The experiments covered a range of refrigerant flow rates from 24 to 84kg/h, and the inlet temperature of cooling water in the condenser and heat source water in the evaporator ranged from 20 to 30$^.\circ}C$The results Showed that the refrigerant flow rate had greater effect on the refrigerating capacities, the compression efficiency and the coefficient of performance of two-stage compression systems than the inlet temperature of heat source water. And all these values were decreased with increasing inlet temperatures of the cooling water. The pressure drops in the evaporator of two-stage compression systems were decreased in proportion to the increase in the inlet temperature of the heat source and cooling water, but it was increased by the refrigerant flow rate.

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Effect of Phosphate and Citrate Salts on the Emulsion Stability of Soy Protein Isolate in the Presence of Calcium (칼슘 존재하에서 인산과 구연산업이 분리대두단백질의 유화 안정성에 미치는 영향)

  • Kim, Yeong-Suk;Yeom, Dong-Min;Hwang, Jae-Gwan
    • The Korean Journal of Food And Nutrition
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    • v.7 no.3
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    • pp.177-182
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    • 1994
  • The effect of phosphate salt (NafHP04) and sodium citrate on the emulsion stability of soy protein isolate (SPI) in the presence of calcium was investigated in terms of salt concentration and addition order. Both phosphate and citrate salts decreased the solubility of SPI despite their pH enhancing effects. Addition of calcium chloride (CaCl2) significantly decreased ES, which showed nearly negligible at more than 3 mM CaCl2 concentration. When Na2HP04 were added in the presence of 5 mM Cac12, 55 greatly increased up to 20mM concentration, above which however ES decreased. It was found that the addition order of Na2HPO4 and CaCl2 affected ES. The addition of phosphate and subsequent CaCl2 exhibited the higher 55 than the reverse order. In both cases, the overall ES profile was found to be nearly similar to the solubility profile of SPI, indicating the positive relationship between solubility and emulsion stability of SPI in the presence of calcium. Similar trend to the phosphate effect on ES was also observed for sodium citrate in the presence of calcium.

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Evaluation on Dynamic Behavior of Friction Welded Joints in Alloy718 to SCM440 using Acoustic Emission Technique (Alloy718/SCM440 마찰용접재의 AE에 의한 동적 거동평가)

  • Kim, Dong-Gyu;Kong, Yu-Sik;Lee, Jin-Kyung
    • Journal of the Korean Society of Industry Convergence
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    • v.22 no.5
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    • pp.491-497
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    • 2019
  • Dissimilar friction welding were produced using 15 mm diameter solid bar in superalloy(Alloy718) to chrome molybdenum steel(SCM440) to investigate their mechanical properties. Consequently, optimal welding conditions were n=2000 rpm, HP=60 MPa, UP=120 MPa, HT=10 sec and UT=10 sec when the metal loss(Mo) is 3.5 mm. Acoustic Emission(AE) technique was applied to analyze the dissimilar friction welding of Alloy718 and SCM440. The relationship between the AE parameters and dissimilar friction welding of both material was discussed. In the case of heating time of 6 sec, 10 sec, 14 sec and 20 sec, 5 AE events per 0.5 seconds and energy about $2.7{\times}10^{10}$ were exhibited in heating time. In upsetting time, resulting in various numbers of events per second and very low energy. The frequency range of the signal generated during the heating time was about 200 kHz. However, the upsetting time resulted in a wide range of signals from very low frequency to high frequency of 500 kHz due to rapid plasticity of the material.

Artificial Intelligent Systems Based on Neural Networks (신경망을 기초로한 인공지능시스템 구현방법)

  • Lee, Gye-Sik
    • Proceedings of the KIEE Conference
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    • 1992.07a
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    • pp.46-48
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    • 1992
  • Through the last 20 years' study, it is a well-known fact that symbolic approach has limitations in generating a new concept from given concepts. Hence, neural networks having a role of associative memory based on dynamical activation of neurons attract AI scientists' attention. In this paper, recent trials for combining neural networks and Artificial Intelligent systems are systematically reviewed and a prototype ENEDB(Experimental Neuro Expert DataBase) system built on HP9000/300 workstation is introduced to show the possibility of using the trials for real applications.

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SIMULINK^{$\circledR}$ Modeling of Turboprop Engine for the Performance Analysis (성능해석을 위한 터보프롭 엔진의 SIMULINK^{$\circledR}$ Modeling)

  • 노홍석;기자영;공창덕
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2001.04a
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    • pp.76-79
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    • 2001
  • SIMULINK^{$\circledR}$를 이용하여 항공기용 터보프롭 엔진을 모델링한 후 현재 KT-1의 추진기관인 PT6A-62 분리축 터보프롭엔진의 성능을 해석하였다. SIMULINK^{$\circledR}$ 모델의 검증을 위하여 상용해석프로그램인 GASTURB 와 비교한 결과 최대오차율 1.07% 이내로 확인되었다. 지상정지 조건에서 블리드 공기유량을 0에서 5%, 보기류 구동에 따른 출력손실을 0에서 20 hp로 가정하고 해석한 결과 축마력은 최대 0.68%감소하며 비연료소모율은 거의 영향을 받지 않음을 알 수 있었다.

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APPLICATION OF HP-DISCONTINUOUS GALERKIN FINITE ELEMENT METHODS TO THE ROTATING DISK ELECTRODE PROBLEMS IN ELECTROCHEMISTRY

  • Okuonghae Daniel
    • Journal of applied mathematics & informatics
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    • v.22 no.1_2
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    • pp.1-20
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    • 2006
  • This paper presents the interior penalty discontinuous Galerkin finite element methods (DGFEM) for solving the rotating disk electrode problems in electrochemistry. We present results for the simple E reaction mechanism (convection-diffusion equations), the EC' reaction mechanism (reaction-convection-diffusion equation) and the ECE and $EC_2E$ reaction mechanisms (linear and nonlinear systems of reaction-convection-diffusion equations, respectively). All problems will be in one dimension.

Development of High frequency Multi-layered Ceramic Chip Inductor (고주파 적층형 칩 인덕터 개발)

  • 강남기;임욱;유찬세
    • Proceedings of the KAIS Fall Conference
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    • 2001.05a
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    • pp.148-150
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    • 2001
  • 본 논문에서는 소결 후 20 ㎛ 정도의 두께를 갖는 ceramic green sheet를 이용하여 초소형(1005) 칩 인덕터를 제작하였다. 인덕터의 패턴을 최적화함에 있어서 HP사의 HFSS(High Frequency Structure Simulator)를 이용하였고 이 과정에서 인덕터의 전기적 특성, 등가회로등을 추출하였다. 칩 인덕터를 제작함에 있어서 모든 적층 공정을 최적화하였다. 실제 제작한 인덕터와 simulation 결과의 관계성을 도출하고 이를 통해 목표 용량을 tuning하였다. 이와 같은 과정을 통해 1-39 nH의 인덕턴스를 갖는 1005크기의 칩 인덕터를 개발하였고, 이를 선진사의 제품과 비교할 때 우수한 전기적 특성을 나타내었다.

Inhibitory Effects of Extracts from Albizzia julibrissin on Gonadal Maturation in a Medaka (Oryzias latipes) (자귀나무 추출물이 송사리(Oryzias latipes) 생식소 성숙에 대한 억제효과)

  • Lee, Eun-Hee;Oh, Sang-Pil;Kim, Myong-Hee;Kim, Kwang-Hyun;Hong, Sang-Hoon;Han, Chang-Hee
    • Journal of Life Science
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    • v.23 no.3
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    • pp.333-340
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    • 2013
  • This study was performed to determine whether stem bark extract containing saponin of Albizzia julibrissin in the diet influences gonadal maturation and spawning in medaka (Oryzias latipes). The crude extraction containing saponin (HaBC) was partially purified from n-BuOH extraction of A. julibrissin stem bark by Diaion HP-20, Silica gel and Sephadex LH-20 chromatographies. We fed diets supplemented with HaBC to medaka. We then studied the effects of the HaBC supplement on the suppression of gonadal maturation and spawning in female medaka that were reared in aquaria with recirculation systems. In the experiment with immature female medaka, the periods of initiation of gonadal maturation and spawning were delayed in the fish that were fed diets supplemented with at least HaBC 20 mg/g-feed. In the experiment with mature female medaka, the fish that were fed diets supplemented with at least HaBC 20 mg/g-feed had lower GSIs than the control diet group did. The results showed that the growth of the immature medaka was not correlated with the amount of supplementation of HaBC in the diet. However, the condition factors (CF) in the medaka that were fed diets supplemented with at least HaBC 20 mg/g-feed were higher than in the medaka fed on the control diet. We concluded that extracts containing saponin from the stem bark of A. julibrissin have the potential to inhibit gonadal maturation in female medaka, but they did not act as growth stimulation. Further studies are required to determine the mechanism of the action.