• Title/Summary/Keyword: HP1

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Production of Hydantoinase from Streptomyces sp. (방선균으로부터 Hydantoinase의 생산)

  • 권태종;이주경;이상훈
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.476-483
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    • 1990
  • In order to investigate hydantoinase-producing strain of the genus Streptomyces, 523 strains of Streptomycee sp. isolated from soils were cultivated in various media and conversion activity of the enzyme was measured to DL-5-phenylhydantoin. A number of strains producing hydantoinase were detected and among them, the strain of Streptomyces sp. Y-183 was selected as a most powerful strain to producing the enzyme. The optimal culture conditions for the production of hydantoinase of the strain were studied, and it was found that almost all hydantoinase activity was produced in the cell fraction. The maximum activity of the enzyme, 17.8 unitstg of dried cells weight, was obtained when the strain was cultured at $30^{\circ}C$ for 72 hr in a medium containing 1.0% of glycerol. 0.5% of yeast extract. 0.5% of soytone, 0.5% of beef extract, 0.6% of KCI, 0.002% of $K_2HP0_4, 0.25% \;of \;CaC0_3, \;0.0002% \; of \; ZnSO_4, \; 0.0002%\; of\; FeS0_4$, and 0.4% of uracil as an inducer, and the pH of culture broth was adjusted ranging from 7.0 to 7.5.

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Expressed Sequence Tag Analysis for Identification and Characterization of Sex-Related Genes in the Giant Tiger Shrimp Penaeus monodon

  • Preechaphol, Rachanimuk;Leelatanawit, Rungnapa;Sittikankeaw, Kanchana;Klinbunga, Sirawut;Khamnamtong, Bavornlak;Puanglarp, Narongsak;Menasveta, Piamsak
    • BMB Reports
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    • v.40 no.4
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    • pp.501-510
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    • 2007
  • Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <$10^{-4}$) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >$10^{-4}$). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. everal full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P<0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).

Identification of Volatile Essential Oil, and Flavor Characterization and Antibacterial Effect of Fractions from Houttuynia cordata Thunb -I. Identification of Volatile Essential Oil Compounds from Houttuynia cordata Thunb -I. Identification of Volatile Essential Oil Compounds from Huttuynia cordata Thunb- (어성초 휘발성 정유성분의 동정과 분획물의 향특성 및 항균활성 -I. 어성초의 휘발성 정유성분의 동정-)

  • Kang, Jung-Mi;Cha, In-Ho;Lee, Young-Kuen;Ryu, Hong-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.26 no.2
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    • pp.209-213
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    • 1997
  • Since Houttuynia cordata is well known as a medicinal herb, due to its antibacterial activity on various microorganisms, present investigation was performed to identify the flavor compounds for volatile essential oil. Volatile essential oil was collected by simultaneous distillation-extraction(SDE), and then the oil components were separated on HP-5 capilliary column$(25m{\times}0.25mm\; i.d.)$ and identified those components by GC-MS. Fifty two compounds were isolated from the volatile essential oil of Houttuynia cordata and forty four were positively identified by GC-MS. The volatile compounds were composed mainly of terpenoids(25 classes), aldehydes(7 classes), alcohols(4 classes), ketones(3 classes), acids(1 class) and miscellaneous compounds(4 classes). Of these, the major compounds were ${\beta}-myrcene$, ${\beta}-ocimene$, decanal, 2-undecanone and geranyl propionate.

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A Position Control System of SRM using Digital Hysteresis Controller (디지털 히스테리시스 제어기를 이용한 SRM의 위치제어시스템)

  • 김민회;백원식;김남훈;최경호;김동희
    • The Transactions of the Korean Institute of Power Electronics
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    • v.7 no.3
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    • pp.253-261
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    • 2002
  • This paper presents an implementation of position control system of Switched Reluctance Motor (SRM) using digital hysteresis controller by TMS320F740 DSP. Although SRM possess several advantages including simple structure and high efficiency, but the control thrive system using power semiconductor device is required to drive this motor. The control drive system increases overall system cost. To overcome this problem and increase the application of SRM, it is needed to develope the servo dave system of SRM. So, the position control system of 1 Hp SRM is developed and evacuated by adaptive switching angle control. The position/speed response characteristics and voltage/current waveforms are presented to prove the capability of SRM for a servo drive application. Moreover, digital hysteresis current controller is developed and evaluated by experimental testing for the purpose of system developmental cost reduction.

Nutrient Requirements for Growth of Lambs under Hot Semiarid Environment

  • Karim, S.A.;Santra, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.665-671
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    • 2003
  • A factorial experiment was conducted to assess nutrient utilization by growing lambs maintained on three levels each of digestible energy (high: HE, medium: ME, low: LE) and protein (high: HP, medium: MP, low: LP) in nine combinations (HEHP, HEMP, HELP, MEHP, MEMP, MELP, LEHP, LEMP, LELP). The experiment was conducted during the hot season in a semiarid location. Daily dry matter intake (DMI) was similar in all the groups in terms of unit body weight or metabolic body size. Digestibility of DM and nitrogen free extract increased (p<0.01) from low to medium and high energy regimen while the CF digestibility followed a reverse trend. The digestibility of crude protein (CP) decreased from high to medium and low protein regimens while it was similar in terms of energy variation. Nitrogen intake was higher in high followed by medium and low protein regime while fecal and urinary nitrogen loss were similar in all the treatment groups. Lambs in all the three levels of protein were in positive N balance and percent N retention was higher (p<0.01) in high followed by medium and low protein levels whereas it was similar in terms of energy variation. Initial body weight was similar in all the groups while final weight, total gain in the experiment and average daily gain (ADG) were higher in high than medium and low energy regimens. It is concluded that crossbred lambs required 75.1 g DM, 9.6 g CP, 6.3 g DCP and 711 KJ DE/kg W $^{0.75}$or 11.0 g CP/MJ DE or 7.2 g DCP/MJ DE for 93 g average daily gain in a hot semiarid environment.

Cooling Performance Deficiency of Air Conditioning System According to Air Quantity Included in Refrigerant (냉매 내 공기혼입에 따른 에어컨 시스템의 냉각성능 저하)

  • Moon, Seong-Won;Min, Young-Bong;Chung, Tae-Sang
    • Journal of Biosystems Engineering
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    • v.34 no.6
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    • pp.470-475
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    • 2009
  • This study was performed to present the diagnosis basis of cooling performance deficiency according to air quantity included in refrigerant of air-conditioner by detecting the temperatures and pressures of refrigerant pipeline. The car air-conditioner of SONATA III (Hyundai motor Co., Korea) was tested by maximum cooling condition at 1500 rpm of engine speed in the room with controlled air condition at $33\sim35^{\circ}C$ and 55~57% RH. Measured variables were temperature differences between inlet and outlet pipe surface of the compressor (Tcom), condenser (Tcon), receive dryer (Trec) and evaporator (Teva), and high pressure (HP) and low pressure (LP) in the refrigerant pipeline, and temperature difference (Tcoo) between inlet and outlet air of the cooling vent of evaporator. Control variables were the refrigerant charging weight and the vacuum degree in the refrigerant pipeline before charging refrigerant. From the test, it was represented that the measuring values of (Tcom), LP and (Tcoo) were enabled to make the diagnosis of cooling performance deficiency according to quantity included in refrigerant of air-conditioner. The ranges of Tcom, LP and Tcoo to make the diagnosis of cooling performance deficiency were respectively less than $55^{\circ}C$, more than 166.7 kPa-g(1.7 kgf/$cm^2$) and less than $13.7^{\circ}C$. In the case of using only external sensors and the condition under the normal performances of air conditioner, it was considered that the ranges of LP and Tcoo to make the diagnosis of cooling performance deficiency were respectively more than 166.7 Pa and less than $12^{\circ}C$.

The Effect of Postural Correction and Subsequent Balloon Inflation in Deformity Correction of Acute Osteoporotic Vertebral Fractures

  • Liu, Hai-Xiao;Xu, Cong;Shang, Ping;Shen, Yue;Xu, Hua-Zi
    • Journal of Korean Neurosurgical Society
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    • v.55 no.6
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    • pp.337-342
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    • 2014
  • Objective : To determine deformity correction by postural correction and subsequent balloon inflation in acute vertebral compression fractures (OVCFs) and to examine the effect of bone mineral density on deformity correction. Methods : A totol of 50 acute OVCFs received balloon kyphoplasty. Lateral radiographs were taken and analyzed at five different time points : 1) preoperative, 2) after placing the patient in prone hyperextended position, 3) after balloon inflation, 4) after deposition of the cement, and 5) postoperative. All fractures were analyzed for height restoration of anterior (Ha), middle (Hm) and posterior (Hp) vertebra as well as Cobb angle and Kyphotic angle. The bone mineral density (BMD) of lumbar spine was measured by dual-energy X-ray absorptiometry. According to the T-score, the patients were divided into two groups which were osteoporosis group and osteopenia group. Results : Postoperative measurements of Ha, Hm and the Cobb angle demonstrated significant reduction of 4.62 mm, 3.66 mm and $5.34^{\circ}$ compared with the preoperative measurements, respectively (each p<0.05). Postural correction significantly increased Ha by 5.51 mm, Hm by 4.35 mm and improved the Cobb angle by $8.32^{\circ}$ (each p<0.05). Balloon inflation did not demonstrate a significant improvement of Ha, Hm or the Cobb angle compared with baseline prone hyperextended. Postural correction led to greater improvements of Ha, Hm and Cobb angle in osteoporosis group than osteopenia group (each p<0.05). Conclusion : In acute OVCFs, the height restoration was mainly attributed to postural correction rather than deformity correction by balloon inflation. BMD affected deformity correction in the process of postural correction.

Measurement of Backscattering Coefficients of Rice Canopy using a Polarimetric Scatterometer System (Polarimetric Scatterometer 시스템을 이용한 벼 군락의 후방산란계수 측정)

  • Hong, Suk-Young;Hong, Jin-Young;Kim, Yi-Hyun;Oh, Yi-Sok
    • Proceedings of the KSRS Conference
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    • 2007.03a
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    • pp.153-157
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    • 2007
  • 본 논문은 지표면 현상의 관측에 날씨의 영향을 거의 받지 않는 마이크로파 L-밴드(1.95 GHz)와 C-밴드(5.3 GHz) scatterometer 시스템을 이용하여 농업과학기술원 내의 논에서 자라는 추청벼를 대상으로 2006년 5월 29일부터 10월 9일까지 생육에 따른 군락의 후방산란계수를 관측한 데이터와 작물의 생육과의 관계를 살펴보고 또한,측정 시스템의 개요,측정 시스템의 보정 방법들을 기술하고자 한다. Scatterometer 시스템의 송 수신기로 HP 8753D 벡터 네트워크 분석기를 사용하며,타워 위에 안테나를 설치하여 3.4 m의 높이에서 측정하도록 하였다. L-밴 드와 C-밴드 scatterometer는 VV-, VH-, HV-, HH-편파를 측정하여 fully polarimetric한 데이터를 얻도록 설계된 레이더시스템으로 입사각을 $30^{\circ}{\sim}60^{\circ}$에서 $10^{\circ}$간격으로 각각 30개의 독립적인 샘플을 측정하여 통계적으로 후방산란계수를 얻었다. 타워에서 발생하는 전파 잡음과 안테나 패턴의 부엽에 의한 지면에서의 수직반사(coherent 성분) 전파를 제거하기 위해 네트워크 분석기의 time gating 기능을 사용하며,55 cm 크기의 trihedral 전파반사기를 보정용 반사기로 사용하고, STCT(single target calibration technique) 방법을 이용하여 시스템을 보정하였다. 측정 결과를 분석하여 주파수, 입사각도, 편파의 변화에 대한 벼의 후방산란 특성과 벼의 생육상태과의 관계를 살펴보았다. L-밴드와 C-밴드 모두 벼의 생육과 밀접한 결과를 나타내었으나,입사각이 작을 때는 C-밴드와의 상관이 높게 나타났고 입사각이 커질수록 L-밴드와의 상관이 높게 나타났다. 편파는 L-밴드 와 C-밴드 모두 hh 편파가,입사각은 50도에서 가장 생육의 변이를 잘 설명하는 것으로 나타났다. 생육 데이터 모두를 이용한 경우보다는 유수형성기 또는 출수기 등 벼 생육의 질적인 변화를 보이는 시기에 따라 나누어 분석하는 것이 변화추이를 더 잘 설명하는 것으로 나타났다.

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Analysis of the Effect of Expected Housing Prices and Liquidity on the Housing Market (유동성과 주택가격의 기대심리가 실질 주택가격에 미치는 영향에 관한 연구)

  • Jeon, Hyeonjin;Kwon, Sunhee
    • Journal of Digital Convergence
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    • v.18 no.11
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    • pp.43-49
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    • 2020
  • The purpose of this study was to analyze factors affecting the housing market by setting household loans and M2, which are liquidity indicators, and the industrial production index reflecting economic fluctuations, as variables, and to determine the effect of expected housing prices. An empirical analysis was conducted based on the data from January 2005 to May 2020, and the HP filter was applied to the real house price as the expected house price variable. As a result of the analysis, it was found that real household loans, real M2, and so on, had an effect on house prices, and expectations for past house prices and house prices increased the house prices in the present period. These results show that even though the liquidity expansion is aimed at revitalizing the economy, it can affect housing prices as well.

Osteopromotive effect of Titanium Reinforced-ePTFE membrane (티타늄강화 차폐막의 골유도 재생 효과)

  • Lee, Jean;Kwon, Young-Hyuk;Park, Joon-Bong;Herr, Yeek;Chung, Jong-Hyuk;Kim, Chong-Kwan
    • Journal of Periodontal and Implant Science
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    • v.34 no.4
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    • pp.711-722
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    • 2004
  • The purpose of this study is to evaluate the regenerated bone histollogically using titanium reinforced ePTFE(TR-ePTFE) membrane and to investigate cell occlusiveness, wound stabilization and tissue integration of TR-ePTFE membrane. Adult male rabbits (mean BW 2kg) and TR9W (W.L.Gore&Associate.INC,USA) were used in this study. Intramarrow penetration defects were surgically created with round carbide bur(HP long #6) on calvaria of rabbits. TR-ePTFE membrane was applied to defect. Then guided bone regeneration was carried out using TR-ePTFE membrane and resorbable suture. At 2,4,8,12 weeks after the surgery, animals were sacrificed. Nondecalcified specimens were processed for histologic analysis. The result and conclusion of this study were as follows: 1. TR-ePTFE membrane had good ability of biocompatibility and cell occlusiveness. 2. space making for guided bone regenerayion was good at TR-ePTFE membrane. 3. Tissue integration was not good at TR-ePTFE membrane. So, wound stabilization was not good. 4. At 8 weeks, 12 weeks after GBR procedure, bone formation was seen. From the above results, TR-ePTFE membrane fixed tightiy on alveolar bone might be recommended for the early bone formation.