• Korean Journal of Microbiology
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• v.43 no.1
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• pp.19-22
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• 2007

Acetohydroxyacid synthase (E.C.2.2.1.6., AHAS) is the enzyme that catalyses the first step in the synthesis of the branched-chain amino acids valine, leucine and isoleucine. The AHAS gene (TIGR access code HI2585) from Heamophilus influenzae was cloned into the bacterial expression vector pET-28a and expressed in the Escherichia coli strain BL21(DE3). The expressed enzyme was purified by $Ni^{2+}-charged$ HiTrap chelating HP column. The purified enzyme appears as a single band on SDS-PAGE with a molecular mass of about 63.9 kDa. The enzyme exhibits absolute dependence on the three cofactors FAD, $MgCl_{2}$ and thiamine diphosphate for activity. Specific activity of purified enzyme has 3.22 unit/mg and optimum activity in the pH 7.5 at $37^{\circ}C$. This enzyme activity has an effect on the buffer. When comparing the enzyme activity against the organic solvent, it followed in type and the difference it is but even from the aqueous solution where the organic solvent is included with the fact that the enzyme activity is maintained.

• Proceedings of the Korea Society for Simulation Conference
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• 2002.05a
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• pp.125-129
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• 2002

실시간 운영체제(Real-Time Operating System: 이하 RTOS라 함) 개발환경에서 제공하는 도구 중에 하나인 RTOS 시뮬레이터는 타겟 하드웨어가 호스트에 연결되어 있지 않아도 호스트에서 응용프로그램의 개발과 디버깅을 가능하게 해주는 타겟 시뮬레이션 환경을 제공해 줌으로서, 개발자로 하여금 빠른 시간 내에 응용프로그램을 개발할 수 있도록 지원하며 하드웨어 개발이 완료되기 전에도 응용프로그램을 개발할 수 있게 해 준다. 그러한 이유로 현재 대부분의 상용 RTOS 개발환경에서는 RTOS 시뮬레이터를 제공하고 있다. 그러나 현재 상용 RTOS 시뮬레이터들은 대부분 RTOS의 기능적인 부분들만 호스트에서 동작하도록 구현되어 있어서 RTOS나 RTOS 응용프로그램이 실제 타겟에서 실행될 때의 실질적인 시간 추정이 불가능하다. 이러한 문제점은 실시간 시스템이 정해진 시간 내에 결과를 출력해야 하는 시스템임을 감안한다면 RTOS 시뮬레이터의 가장 큰 결점이 되기 때문에 실행시간 추정 기능을 가지면서 실용화도 가능한 RTOS 시뮬레이터가 필요하다. 본 연구에서는 이러한 문제점을 해결하여 RTOS와 RTOS 응용프로그램이 실제 타겟에서 처리될 때의 실행시간 추정이 가능하고 상용화가 가능한 기계 명령어 기반(machine instruction-based)의 RTOS 시뮬레이터를 연구 개발하였다. 나아가 실행시간의 주요 요소인 파이프라인과 캐쉬의 영향도 고려함으로서 실행시간 추정의 정확도를 향상시켰다 본 연구에서 사용된 RTOS는 한국전자통신연구원(ETRI)에서 2000년에 개발된 Q+이고, Q+가 동작하는 타겟 하드웨어는 ARM 계열의 StrongARM SA-110 마이크로프로세서와 21285 주제어기가 장착된 EBSA-285 보드이다. 측정하면서 수행하였다. 검증 결과 random 상태에서는 문헌자료에 부합되는 예측결과를 보여주었으나, intermediate와 constant 상태에서는 문헌보다 다소 낮은 속도를 보여주었다 이러한 속도차는 추후 현장 데이터를 수집하여 보다 실질적인 검증을 통하여 조정되어야 할 것으로 판단된다.지발광(1.26초)보다 구애발광(1.12초)에서 0.88배 감소하였고, 암컷에서 정지발광(2.99초)보다 구애발광(1.06초)에서 0.35배 감소하였다. 발광양상에서 발광주파수는 수짓의 정지발광에서 0.8 Hz, 수컷 구애발광에서 0.9 Hz, 암컷의 정지발광에서 0.3 Hz, 암컷의 구애발광에서 0.9 Hz로 각각 나타났다. H. papariensis의 발광파장영역은 400 nm에서 700 nm에 이르는 모든 영역에서 확인되었으며 가장 높은 첨두치는 600 nm에 있고 500에서 600 nm 사이의 파장대가 가장 두드러지게 나타났다. 발광양상과 어우러진 교미행동은 Hp system과 같은 결과를 얻었다.하는 방법을 제안한다. 즉 채널 액세스 확률을 각 슬롯에서 예약상태에 있는 음성 단말의 수뿐만 아니라 각 슬롯에서 예약을 하려고 하는 단말의 수에 기초하여 산출하는 방법을 제안하고 이의 성능을 분석하였다. 시뮬레이션에 의해 새로 제안된 채널 허용 확률을 산출하는 방식의 성능을 비교한 결과 기존에 제안된 방법들보다 상당한 성능의 향상을 볼 수 있었다., 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.8
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• pp.1068-1075
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• 2000

A study involving nutrient balances and radioisotope labeling techniques was undertaken to study energy and protein metabolism, and glucose kinetics of female crossbred Etawah goats, using 12 weaned (BW $14.0{\pm}2.0kg$), 12 late pregnant (BW $27.8{\pm}1.8kg$) and 12 first lactation does (BW $25.0{\pm}5.0kg$). Each class of animal was randomly allotted into 3 dietary treatment groups R1, R2 and R3, that received 100%, 85%, and 70% of ad libitum feed. The rations offered were pellets containing 21.8% CP and 19.3 MJ GE/kg, except for the lactating does who received pellets (17.2% CP and 18.9 MJ GE/kg) and fresh Penisetum purpureum grass. Energy and nitrogen balance studies were conducted during a two-week trial. Daily heat production (HP, estimated by the carbon dioxide entry rate technique), glucose pool and flux were measured. Equations were found for metabolizable energy (ME) and protein intake (IP) requirements for growing goats: ME (MJ/d)=1.87+0.55 RE-0.001 ADG+0.044 RP $(R^2=0.89)$ and IP (g/d)=48.47+2.99 RE+0.029 ADG+0.79 RP $(R^2=0.90)$; for pregnant does: ME (MJ/d)=5.92+0.96 RE-0.002 ADG+0.003 RP $(R^2=0.99)$ and IP (g/d)=58.34+5.41 RE+0.625 ADG-0.30 RP $(R^2=0.98)$; and for lactating does: ME (MJ/d)=4.23+0.713 RE+0.003 ADG+0.006 RP+0.002 MY $(R^2=0.86)$; IP (g/d)=84.05-5.36 RE+0.055 ADG-0.16 RP+0.068 MY $(R^2=0.45)$, where RE is retained energy (MJ/d), ADG is average daily gain in weight (g/d), RP is retained protein (g/d) and MY is milk yield (ml/d). ME and IP requirements for maintenance for growing goats were 0.46 MJ/d.kg $BW^{0.75}$ and 7.43 g/d.kg $BW^{0.75}$, respectively. Values for the pregnant and lactating does were in the same order, 0.55 MJ/d.kg $BW^{0.75}$ and 11.7 g/d.kg $BW^{0.75}$, and 0.50 MJ/d.kg $BW^{0.75}$ and 10.8 g/d.kg $BW^{0.75}$, respectively. Milk protein ranged from 3.06 to 3.5% and milk fat averaged 5.2%. Glucose metabolism in Etawah crossbred female goat is active, but glucose flux is low compared to temperate ruminant breeds which may implicate its role to support production.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.205-210
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• 1999

16brio cholerne is an important pathogenic organism that causes dimhea in human beings. V ciaoleroe KNIH002 was isolated from patients suffering with dian.heal disease in Korea. From Southern hybridization using the amplified PCR product of 307 bp as a probe. which was obtained from PCR reaction using primer detecting cholera toxin gene, we have found that the c b gene located in 4.5-kb fragmenl double digested with Pstl and BgllI of the chromosome. Therefore, we made mini-libraries of the isolate using PstI and Bgm restriction endonuclease and pBluescript SKU(+) vector. As a result. we cloned 4.5-kb PstI-BglII fragment containing the c a gene encoding a cholera toxin from the constructed mini-libraries of V olzolerae KNlH002 by colony hybridization using the same probes. This recombinant plasmid was named pCTX75. E. coii XL1- Blue harboring pCTX75 showed the cytotoxicity on Chinese Hamster Ovary cells. From the sequencing of he cloned recombinant plasmid, we confinned that it has virulence gene cassette consisting of ace, zot, ctx.4 and cf"~B gene. The ace and zot genes were composed of 291 hp and 1.200 bp with ATG initiation codon and TGA lennination codon, respectively. Nucleotide sequence of the ace gene exhibited 100% identity with that of V cholera E7946 El Tor Ogawa strains. But, nucleolide and amino acid sequence comparison of the zot gene exhibited 99% and 98.8% identity with that of V cholerae 395 Classical Ogawa stram, respectively. Specially. the Ala-100, Ala-272 and Ala-281 sites of Zoi polypeptide presented in V choleme 395 Classical Ogawa strain are replaced by Val in V cholerae KNIH002.

• Korean Journal of Weed Science
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• v.16 no.1
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• pp.42-53
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• 1996

The tolerant and susceptible rice cultivars to oxyfluorfen were selected from 400 rice cultivars in a laboratory, and tested in comparison with barnyardgrass, a typical oxyfluorfen susceptible weed. The responses to oxyfluorfen in the different levels of calli, cells and protoplasts of the rice cultivars were investigated. $I_{50}$ value of the tolerant rice cultivars was about $10^{-4}M$, whereas that of the susceptible rice cultivars and barnyardgrass was about $10^{-6}M$, showing significant difference between the two groups. The growth rate of calli segregated from the tolerant rice cultivars was higher than that from the susceptible rice cultivars and barnyardgrass by treatment of oxyfluorfen to the calli. The growth rate of suspension-cultured cells of the tolerant rice cultivars was higher than that of the susceptible rice cultivars and barnyardgrass after treatment of oxyfluorfen. The viability of protoplasts from the tolerant rice cultivars was higher than that from the susceptible rice cultivars and barnyardgrass after one or two hours of oxyfluorfen treatment. The intactness of protoplasts from the tolerant rice cultivars was also higher than that form the susceptible rice cultivars and barnyardgrass.

• Proceedings of the Safety Management and Science Conference
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• 2003.05a
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• pp.43-51
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• 2003

제품 설계 및 디자인의 과정이 고도로 높은 기술력을 바탕으로 이루어지고 있으며, 국내의 기업체도 우수한 기술력과 높은 품질로 경쟁력을 키우며 다양한 고객의 요구에 대응하여 고객만족을 꾀하여야 한다 이의 기반이 되는 제품의 품질과 사양은 설계에서 시작되는데 아직 국내의 많은 기업들은 설계 및 제작 단계에서 많은 시간과 비용을 낭비하고 있다. 3D Modeling Solution은 설계오류가 적으며 시각적인 설계를 할 수 있어 최소의 인력으로 제품을 설계할 수 있는 장점이 있지만 너무 많은 기능으로 인해 사용자가 쉽게 적용하고 사용하기 어려운 단점을 가지고 있다. 본 연구에서는 이러한 산업현장의 어려움을 덜기 위해 3D 전용 Modeling Solution에 사용자가 쉽게 부품을 조림할 수 있는 엔진을 접목시켜 누구나 사용가능하고 신속한 신제품 개발이 이루어지도록 하였다. 본 연구에서는 Autodesk사의 Inventor와 Microsoft Visual Basic으로 Inventor에서 제공하고 있는 API함수를 이용하여 조립자동화를 위한 조립조건 생성, 조립자동화, 부품 재질변경, 수동조립 그리고 부품의 DB화를 구현하였다. 이 프로그램은 조립조건 설정 폼을 이용하여 부품의 조립속성을 생성하고 부품조립 폼을 이용하여 조립자동화를 실행할 수 있도록 하였다. 또한 모든 부품을 Database화 하여 부품을 손쉽게 탐색할 수 있으며, 추후에도 언제든지 재사용이 가능하여 제품설계 효율성을 극대화 할 수 있다. 현장 적용 시 신속한 신제품 개발과 품질의 우수성으로 고객만족을 꾀할 수 있으며, 시간과 비용을 동시에 줄여 경쟁사와의 경쟁우위를 높이는 해결책이 될 수 있다.-110 마이크로프로세서와 21285 주제어기가 장착된 EBSA-285 보드이다. 측정하면서 수행하였다. 검증 결과 random 상태에서는 문헌자료에 부합되는 예측결과를 보여주었으나, intermediate와 constant 상태에서는 문헌보다 다소 낮은 속도를 보여주었다 이러한 속도차는 추후 현장 데이터를 수집하여 보다 실질적인 검증을 통하여 조정되어야 할 것으로 판단된다.지발광(1.26초)보다 구애발광(1.12초)에서 0.88배 감소하였고, 암컷에서 정지발광(2.99초)보다 구애발광(1.06초)에서 0.35배 감소하였다. 발광양상에서 발광주파수는 수짓의 정지발광에서 0.8 Hz, 수컷 구애발광에서 0.9 Hz, 암컷의 정지발광에서 0.3 Hz, 암컷의 구애발광에서 0.9 Hz로 각각 나타났다. H. papariensis의 발광파장영역은 400 nm에서 700 nm에 이르는 모든 영역에서 확인되었으며 가장 높은 첨두치는 600 nm에 있고 500에서 600 nm 사이의 파장대가 가장 두드러지게 나타났다. 발광양상과 어우러진 교미행동은 Hp system과 같은 결과를 얻었다.하는 방법을 제안한다. 즉 채널 액세스 확률을 각 슬롯에서 예약상태에 있는 음성 단말의 수뿐만 아니라 각 슬롯에서 예약을 하려고 하는 단말의 수에 기초하여 산출하는 방법을 제안하고 이의 성능을 분석하였다. 시뮬레이션에 의해 새로 제안된 채널 허용 확률을 산출하는 방식의 성능을 비교한 결과 기존에 제안된 방법들보다 상당한 성능의 향상을 볼 수 있었다., 인삼이 성장될 때 부분적인 영양상태의 불충분이나 기후 등에 따른 영향을 받을 수 있기 때문에 앞으로 이에 대한 많은 연구가 이루어져야할 것으로 판단된다.

• Hip & pelvis
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• v.34 no.1
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• pp.25-34
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• 2022

Purpose: Mortality rates following hip fracture surgery have been well-studied. This study was conducted to examine mortality rates in asymptomatic patients presenting for treatment of acute hip fractures with concurrent positive COVID-19(+) tests compared to those with negative COVID-19(-) tests. Materials and Methods: A total of 149 consecutive patients undergoing hip fracture surgery during the COVID-19 pandemic at two academic medical centers were reviewed retrospectively. Patients were divided into two groups for comparative analysis: one group included asymptomatic patients with COVID-19+ tests versus COVID-19- tests. The primary outcome was mortality at 30-days and 90-days. Results: COVID-19+ patients had a higher mortality rate than COVID-19- patients at 30-days (26.7% vs 6.0%, P=0.005) and 90-days (41.7% vs 17.2%, P=0.046) and trended towards an increased length of hospital stay (10.1±6.2 vs 6.8±3.8 days, P=0.06). COVID-19+ patients had more pre-existing respiratory disease (46.7% vs 11.2%, P=0.0002). Results of a Cox regression analysis showed an increased risk of mortality at 30-days and 90-days from COVID-19+ status alone without an increased risk of death in patients with pre-existing chronic respiratory disease. Conclusion: Factors including time to surgery, age, preexisting comorbidities, and postoperative ambulatory status have been proven to affect mortality and complications in hip fracture patients; however, a positive COVID-19 test result adds another variable to this process. Implementation of protocols that will promote prompt orthogeriatric assessments, expedite patient transfer, limit operating room traffic, and optimize anesthesia time can preserve the standard of care in this unique patient population.

• Food Science and Preservation
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• v.22 no.2
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• pp.218-224
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• 2015

Crickets have been used as crude drug for treating fever and hypertension in East Asia. This study was carried out to investigate the quality characteristics such as the microbial and nutrient contents of crickets (Gryllus bimaculatus) prepared with various processing conditions for use as food. These conditions included the lyophilization process (LP), hot-air process (HP, $90^{\circ}C$ for 7 hr), roasting process (RP, $160^{\circ}C$ for 40 min), and frying process (FP, $180^{\circ}C$ for 30 sec). The total bacterial population of the crickets was 5~7 log CFU/g, but Coliform and E. coli were not detected. The major fatty acids in all the samples were palmitic acid (C16:0), oleic acid (C18:1), and linoleic acid (C18:2). The level of polyunsaturated fatty acids was highest (63.55 g/100 g) in the LP-processed crickets. As for the amino acid content, the glutamic acid level was highest in all the samples, and the RP and FP decreased by 12.01% and 53.88%, respectively, compared to that of the LP. The mineral contents were highest in the LP-processed crickets. Hg was detected at about 1.0 ppb in all the samples, but its level was lower than the residue tolerance level in the Korean Food Code. Such conditions should be considered to better understand the quality characteristics of crickets in food processing.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.131.1-131.1
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• 2010

This study was conducted for engineering optimization for the gasification process which is the key factor for success of Taean IGCC gasification plant which has been driven forward under the government support in order to expand to supply new and renewable energy and diminish the burden of the responsibility for the reduction of the green house gas emission. The gasification process consists of coal milling and drying, pressurization and feeding, gasification, quenching and HP syngas cooling, slag removal system, dry flyash removal system, wet scrubbing system, and primary water treatment system. The configuration optimization is essential for the high efficiency and the cost saving. For this purpose, it was designed to have syngas cooler to recover the sensible heat as much as possible from the hot syngas produced from the gasifier which is the dry-feeding and entrained bed slagging type and also applied with the oxygen combustion and the first stage cylindrical upward gas flow. The pressure condition inside of the gasifier is around 40~45Mpg and the temperature condition is up to $1500{\sim}1700^{\circ}C$. It was designed for about 70% out of fly ash to be drained out throughout the quenching water in the bottom part of the gasifier as a type of molten slag flowing down on the membrane wall and finally become a byproduct over the slag removal system. The flyash removal system to capture solid particulates is applied with HPHT ceramic candle filter to stand up against the high pressure and temperature. When it comes to the residual tiny particles after the flyash removal system, wet scurbbing system is applied to finally clean up the solids. The washed-up syngas through the wet scrubber will keep around $130{\sim}135^{\circ}C$, 40~42Mpg and 250 ppmv of hydrochloric acid(HCl) and hydrofluoric acid(HF) at maximum and it is turned over to the gas treatment system for removing toxic gases out of the syngas to comply with the conditions requested from the gas turbine. The result of this study will be utilized to the detailed engineering, procurement and manufacturing of equipments, and construction for the Taean IGCC plant and furthermore it is the baseline technology applicable for the poly-generation such as coal gasification(SNG) and liquefaction(CTL) to reinforce national energy security and create new business models.

• Journal of the Korea Society for Simulation
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• v.29 no.1
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• pp.57-69
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• 2020

Many researchers have recommended that DBR scheduling would be an efficient method to maintain the balance of their workload among many processes in the general flow shop. However, as product variety has increased in recent years, the process has become more complex and requires the re-entrance of raw materials and work in process. The re-entrant line has known for the complex manufacturing process that raw materials are repeatedly processed on the same machine. This study reviews the applicability of DBR against the re-entrant manufacturing line due to the distinguishing characteristics and the higher complexity caused by multiple visits of a job into the identical process. In order to apply the DBR method to the re-entrant process, the main idea is to reconstruct re-entrant process into a virtual flow process(loop) that has a single bottleneck. This study discusses the following two questions. First, DBR is also superior to traditional scheduling methods against re-entrant manufacturing line. And how we structure and detect the system bottleneck (or sub-bottleneck) through drum-buffer-rope concepts. To answer the above questions, we experimented and analyzed the effects of the applicability of DBR under the general re-entrant process model(TRC, Technology Research Center). As a result, we have identified a balance between loops for cycle time and work in process.