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Evaluation of Arabinofuranosidase and Xylanase Activities of Geobacillus spp. Isolated from Some Hot Springs in Turkey

  • Sabriye, Canakci;Inan, Kadriye;Murat, Kacagan;Belduz, Ali Osman
    • Journal of Microbiology and Biotechnology
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    • v.17 no.8
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    • pp.1262-1270
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    • 2007
  • Some hot springs located in the west of Turkey were investigated with respect to the presence of thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 16 of the isolates belonged to the genus Geobacillus and grew optimally at about $60^{\circ}C$ on nutrient agar. 16S rRNA gene sequence analysis showed that these isolates resembled Geobacillus species by ${\ge}97%$, but SDS-PAGE profiles of these 16 isolates differ from some of the other species of the genus Geobacillus. However, it is also known that analysis of 16S rRNA gene sequences may be insufficient to distinguish between some species. It is proposed that recN sequence comparisons could accurately measure genome similarities for the Geobacillus genus. Based on recN sequence analysis, isolates 11, IT3, and 12 are strains of G stearothermophilus; isolate 14.3 is a strain of G thermodenitrificans; isolates 9.1, IT4.1, and 4.5 are uncertain and it is required to make further analysis. The presence of xylanase and arabinofuranosidase activities, and their optimum temperature and pH were also investigated. These results showed that 7 of the strains have both xylanase and arabinofuranosidase activities, 4 of them has only xylanase, and the remaning 5 strains have neither of these activities. The isolates 9.1, 7.1, and 3.3 have the highest temperature optima ($80^{\circ}C$), and 7.2, 9.1, AO4, 9.2, and AO17 have the highest pH optima (pH 8) of xylanase. Isolates 7.2, AO4, AC15, and 12 have optimum arabinofuranosidase activities at $75^{\circ}C$, and only isolate AC15 has the lowest pH of 5.5.

Chemical Characterization of Neutral Extracts Prepared by Treating Pinus radiata Bark with Sodium Bicarbonate

  • MUN, Ji Sun;KIM, Hwan Chul;MUN, Sung Phil
    • Journal of the Korean Wood Science and Technology
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    • v.48 no.6
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    • pp.878-887
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    • 2020
  • A neutral extract (NE), that is soluble in cold water and has excellent antioxidant activity, from Pinus radiata pine bark was prepared by sodium bicarbonate treatment, and its chemical characteristics were investigated. NE was prepared by treating P. radiata bark with 0.8% NaHCO3 aqueous solution with a 5 : 1 liquor-to-bark ratio at boiling temperature for 1 h, resulting in 44% yield and final pH of 6.66. The yield of NE was 11% higher than that of the hot water extract (HWE) due to the increase in the solubility of polyphenols, the main component in the bark, by NaHCO3 treatment. NE was characterized through FT-IR, NMR, and MALDI TOF MS analyses. The results indicated that NE is mostly composed of proanthocyanidins (PAs) consisting of procyanidin (PC) units. The acetylated neutral extract (Ac-NE) had weight average molecular weight (${\bar{M}}w$) of 5,300 Da. The Ac-NE had wide molecular weight distribution and its polydispersity (${\bar{M}}w/{\bar{M}}n$) was 6 times higher than that of pure PA. The antioxidant activity of NE was determined by 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and showed that NE had comparable antioxidant activity with pure PA.

Equilibrium Removal of Pb (II) Ions from Aqueous Solution onto Oxidized-KOH-Activated Carbons

  • Fathy, Nady A.;El-Sherif, Iman Y.
    • Carbon letters
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    • v.12 no.1
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    • pp.1-7
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    • 2011
  • In the present study, the removal of Pb (II) ions on oxidized activated carbons (ACs) was investigated. ACs were derived from activation of indigenous cotton stalks waste with potassium hydroxide (KOH) in two-stage process. The KOH-ACs were subjected to liquid-phase oxidation with hot $HNO_3$ and one untreated sample was included for comparison. The obtained carbons were characterized by Fourier transform infrared (FTIR), slurry pH and $N_2$-adsorption at 77 K, respectively. Adsorption capacity of Pb (II) ions on the resultant carbons was determined by batch equilibrium experiments. The experimental results indicated that the oxidation with nitric acid was associated with a significant increase in mass of yield as well as a remarkable reduction in internal porosity as compared to the untreated carbon. The AC-800N revealed higher adsorption capacity than that of AC-800, although the former sample exhibited low surface area and micropore volume. It was observed that the adsorption capacity enhancement attributed to pore widening, the generation of oxygen functional groups and potassium containing compounds leading to cation-exchange on the carbon surface. These results show that the oxidized carbons represented prospective adsorbents for enhancing the removal of heavy metals from wastewater.

Analysis on Electric Shock Current in DC Electricity (직류환경에서 인체에 흐르는 감전전류 분석)

  • Lee, Jin-Sung;Kim, Hyosung
    • The Transactions of the Korean Institute of Power Electronics
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    • v.21 no.3
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    • pp.254-259
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    • 2016
  • Recently, DC distribution systems have become a hot issue because of the increase in digital loads and DC generation systems according to the expansion of renewable energy technologies. To obtain the practical usage of DC electricity, safety should be guaranteed. The main concerns for safety are twofold: one side is human protection against electric shocks, and the other is facility protection from short faults. "Effects of current on human beings and livestock" (IEC 60479) defines a human body impedance model in electric shock conditions that consists of resistive components and capacitive components. Although the human body impedance model properly works in AC electricity, it does not well match with the electric shock behavior in DC electricity. In this study, the contradiction of the human body impedance model defined by IEC 60479 in case of DC electricity is shown through experiments for the human body. From the analysis of experimental results, a novel unified human body impedance model in electric shock conditions is proposed. This model consists of resistive components, capacitive components, and an inductance component. The proposed human impedance model matches well for AC and DC electricity environments in simulation and experiment.

Corrosion Mechanisms of New Wrought Mg-Zn Based Alloys Alloying with Si, Ca and Ag

  • Ben-Hamu, G.;Eliezer, D.;Shin, K.S.;Wagner, L.
    • Corrosion Science and Technology
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    • v.7 no.3
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    • pp.152-157
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    • 2008
  • New wrought magnesium alloys have increasingly been developed in recent years for the automotive industry due to their high potential as structural materials for low density and high strength/weight ratio demands. However, their poor mechanical properties and low corrosion resistance have led to a search for new kinds of magnesium alloys with better strength, ductility, and high corrosion resistance. The main objective of this research is to investigate the corrosion behaviour of new magnesium alloys: Mg-Zn-Ag (ZQ), Mg-Zn-Mn-Si (ZSM) and Mg-Zn-Mn-Si-Ca (ZSMX). These ZQ6X, ZSM6X1, and ZSM651+YCa alloys were prepared using hot extrusion. AC, DC polarization and immersion tests were carried out on the extruded rods. Microstructure was examined using optical and electron microscopy (SEM) and EDS. The addition of silver decreased the corrosion resistance. The additions of silicon and calcium also affected the corrosion behaviour. These results can be explained by the effects of alloying elements on the microstructure of Mg-Zn alloys such as grain size and precipitates caused by the change in precipitation and recrystallisation behaviour.

An Electrochemical Evaluation on the Corrosion Resistance of a Al Alloy (주조용 Al합금의 내식성에 관한 전기화학적 평가)

  • Youn Dae-Hyun;Lee Myung-Hoon;Kim Ki-Joon;Moon Kyung-Man
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.5
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    • pp.495-501
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    • 2005
  • Al is a active metal that owes its resistance to a thin, protective, barrier oxide surface layer, which is stable in air and neutral aqueous solution. Thus Al alloys are widely used in architectural trim. cold & hot-water storage vessels and piping. However Al and most of its alloy may corrode with some forms such as pitting corrosion, intergranular corrosion and galvanic corrosion in the case of exposure to various industrial and marine atmosphere. Therefore a correct evaluation of corrosion resistance for their Al and Al alloys may be more important in a economical point of view. In this study. a relative evaluation of corrosion resistance for three kinds of Al alloys such as ALDC2, ALDC3, and ALDC8 series was carried out with electrochemical method. There is a tendency that corrosion potential is shifted to positive or negative direction by alloying components regardless of corrosion resistance. Moreover the data of corrosion properties obtained from cathodic Polarization curve, cyclic voltammogram and AC. DC impedance respectively showed a good correspondence each other against the corrosion resistance but variation of corrosion potential. passivity current density of anodic polarization curve and corrosion current density by Tafel extrapolation and Stern-Geary method didn't correspond with not only each other but also considerably the data of corrosion properties discussed above. Therefore it is suggested that an optimum electrochemical evaluation for corrosion resistance of Al alloy is to calculate the diffusion limiting current density of cathodic polarization curve, impedance of AC or DC and polarization resistance of cyclic voltammogram.

Evaluation of the Characteristics of the Aluminum Alloy Casting Material by Heat Treatment (AC8A 알루미늄합금 주조재의 열처리에 의한 특성 평가)

  • Lee, Syung Yul;Park, Dong Hyun;Won, Jong Pil;Kim, Yun Hae;Lee, Myung Hoon;Moon, Kyung Man;Jeong, Jae Hyun
    • Corrosion Science and Technology
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    • v.11 no.6
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    • pp.280-285
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    • 2012
  • Aluminum is on active metal, but it is well known that its oxide film plays a role as protective barrier which is comparatively stable in air and neutral aqueous solution. Thus, aluminum alloys have been widely applied in architectural trim, cold & hot-water storage vessels and piping etc., furthermore, the aluminum alloy of AC8A have been widely used in mold casting material of engine piston because of its properties of temperature and wear resistance. In recent years, the oil price is getting higher and higher, thus the using of low quality oil has been significantly increased in engines of ship and vehicle. Therefore it is considered that evaluation of corrosion resistance as well as wear resistance of AC8A material is also important to improve its property and prolong its lifetime. In this study, the effect of solution and tempering heat treatment to corrosion and wear resistance is investigated with electrochemical method and measurement of hardness. The hardness decreased with solution heat treatment compared to mold casting condition, but its value increased with tempering heat treatment and exhibited the highest value of hardness with tempering heat treatment temperature at $190^{\circ}C$ for 24hrs. Furthermore, corrosion resistance increased with decreasing of the hardness, and decreased with increasing of the hardness reversely. As a result, it is suggested that the optimum heat treatment to improve both corrosion and wear resistance is tempering heat treatment temperature at $190^{\circ}C$ for 16hrs.

AC-DC Transfer Characteristics of a Bi-Sb Multijunction Thermal Converter (Bi-Sb 다중접합 열전변환기의 교류-직류 변환 특성)

  • 김진섭;이현철;함성호;이종현;이정희;박세일;권성원
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.35D no.11
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    • pp.46-54
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    • 1998
  • A planar Bi-Sb multijunction thermal converter, which is consisted of a linear or bifilar thin film NiCr-heater and a thin film Bi-Sb thermopile, has been fabricated, and its ac-dc transfer characteristics were examined in a frequency range from 10 Hz to 10 KHz. In order to increase the thermal sensitivity and to decrease the ac-dc transfer error of a thermal converter, the heater and the hot junctions of a thermopile were prepared on a Si$_3$N$_4$/SiO$_2$/Si$_3$N$_4$-diaphragm which acts as a thermal isolation layer, and the cold junctions on the Si$_3$N$_4$/SiO$_2$/Si$_3$N$_4$-thin film supported with the silicon rim which functions as a heat sink. The respective thermal sensitivities in air and in a vacuum of the converter with a built-in bifilar heater were about 14.0 ㎷/㎽ and 54.0 ㎷/㎽, and the ac-dc voltage and the current transfer difference ranges in air were about $\pm$0.60 ppm and $\pm$0.11 ppm, respectively, indicating that the ac-dc transfer accuracy of the converter are much higher than that of a commercial 3-dimensional multijunction thermal converter. However, the output thermoelectric voltage fluctuation of the converter was rather high.

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Development of equipment for tumor cauterization

  • Hoshino, Hirokazu;Ochiai, Makoto;Sakasegawa, Aya;Hayakawa, Yoshinori
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.474-477
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    • 2002
  • Equipment to cauterize tumors by an electrically heated Kanthal wire is under development. The wire( alloy of iron, chromium and Aluminum) keeps sufficient strength up to 1400 degrees in Celsius. Although AC 50Hz current source is used in the prototype experiment, RF current will be used in future. The diameter of the Kanthal wire was 0.3 mm which was connected to Kanthal wire of 0.8 mm. The thicker wire was used as a leading wire. The possibility of application of the heating wire in combination with an ultrasound endoscope was determined, where ultrasound endoscope is to be used to monitor the location on the wire and an extent of a tumor in digestive organs. This procedure requires the wire to be applied inside ultrasound transmitting media. First, the wire was applied in the degassed water in which a chicken liver sample was submerged. The wire, however, burned out in water soon after it became red-hot at 12 A. The reason is that large current is required for the wire to become red-hot due to strong convection. Starch paste of 3 weight percent was employed instead of water. This made the wire red-hot approximately at 6 A, showing the increased viscosity of the starch decreased the convection and the wire was cover by the steam. The liver sample was cauterized successively, while the location of the wire and the liver was monitored by an ultrasound diagnosis equipment outside the plastic vessel of the starch paste.

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Output Control Simulation of PV-AF Generation System under Various Weather Conditions (다양한 기상조건하에서의 AF기능을 갖는 태양광발전시스템의 출력제어 시뮬레이션)

  • Seong, Nak-Gueon;Park, Min-Won;Yu, In-Keun
    • Proceedings of the KIEE Conference
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    • 2002.07b
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    • pp.1364-1366
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    • 2002
  • The Photovoltaic(PV) generation system is a promising source of energy for the future. Since the need for renewable energy has been increased, the research of PV generation system has also been progressed. Recently, cost down of PV generation system has been accomplished and practical technologies of the solar energy developed, Moreover, grid connected PV generation system are becoming actual and general. Operational technology of the grid connected PV generation system is being a hot issue. Power output of PV system is directly affected by wether conditions. When AC power supply is needed, power conversion by an inverter and a MPPT control are necessary. In this paper, for stability improvement of PV generation system. Active filter(AF) function is added to PV generation system, and simulations of PV-AF system under various weather conditions are performed.

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