• Title/Summary/Keyword: HOST

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Ultrastructural Study on the Haustorial Cells of Cuscuta australis R. Brown in the Region of the Host Parenchyma (기주식물의 유조직 속에서 생장하는 실새삼(Cuscuta australis R. Brown) 흡기세포의 미세구조)

  • 이규배
    • Journal of Plant Biology
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    • v.34 no.2
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    • pp.129-136
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    • 1991
  • Two cell types, tip cells and hyphal cells, were found at the front of Cuscuta australis endophyte growing into the stem parenchyma of the host plant, Trifolium repens. Each tip cell developed into an elongate, filamentous hypha. The cells of both types possessed a dense cytoplasm including abundant organelles and enlarged nuclei with the deeply lobed envelope. The unevenly thick walls were observed in certain tip cells. The wall penetrated through the middle lamellae of the host cells and engulfed the debris of broken host cells. Some front cells had the plasmalemma-wall invaginations, which increased the surface area and would facilitate material uptake from the host No plasmodesmata between the host and parasite cells were found; instead, an apoplastic continuity was established by fused cell walls at the interface of the two partners. The apoplast was thought to be the main route for water and nutrients transport.nsport.

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Infectivity of Orientia tsutsugamushi to Various Eukaryotic Cells and Their Cellular Invasion Mechanism (Orientia tsutsugamushi의 유핵세포내 감염능 분석 및 기전)

  • Ihn, Kyung-Soo;Han, Seung-Hoon;Kim, Hang-Rae;Seong, Seung-Yong;Kim, Ik-Sang;Choi, Myung-Sik
    • The Journal of the Korean Society for Microbiology
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    • v.34 no.5
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    • pp.435-443
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    • 1999
  • Orientia tsutsugamushi is obligate intracellular bacterium that grows within the cytoplasm of the eukaryotic host cells. Therefore capability of the attachment, entry into the host cell and intracellular survival should be critical process for oriential infection. In this study we investigated the cellular invasion mechanism of Orientia tsutsugamushi and the role of transmembrane heparan sulfate proteoglycan, which binds diverse components at the cellular microenvironment and is implicated as host cell receptors for a variety of microbial pathogens. First of all Orientia tsutsugamushi can invade a wide range of nonprofessional phagocytic cells including fibroblast, epithelial cells and endothelial cells of various host species, including Band T lymphocytes. Thus, it was postulated that the attachment of O. tsutsugamushi requires the recognition of ubiquitous surface structures of many kinds of host cells. Treatments with heparan sulfate and heparin inhibited the infection of Orientia tsutsugamushi in dose-dependent manner for L cell, mouse fibroblast, whereas other glycosaminoglycans such as chondroitin sulfate had no effect. Collectively, these findings provide strong evidence that initial interaction with heparan sulfate proteoglycan is required for the oriential invasion into host cells.

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Interactions between secreted GRA proteins and host cell proteins across the parasitophorous vacuolar membrane in the parasitism of Toxoplasma gondii

  • Ahn, Hye-Jin;Kim, Sehra;Kim, Hee-Eun;Nam, Ho-Woo
    • Parasites, Hosts and Diseases
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    • v.44 no.4 s.140
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    • pp.303-312
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    • 2006
  • Interactions between GRA proteins of dense granules in Toxoplasma gondii and host cell proteins were analyzed by yeast two-hybrid technique. The cMyc-GRA fusion proteins expressed from pGBKT7 plasmid in Y187 yeast were bound to host cell proteins from pGADT7-Rec-HeLa cDNA library transformed to AH109 yeast by mating method. By the selection procedures, a total of 939 colonies of the SD/-AHLT culture, 348 colonies of the $X-\alpha-gal$ positive and PCR, 157 colonies of the $X-\beta-gal$ assay were chosen for sequencing the cDNA and finally 90 colonies containing ORF were selected to analyze the interactions. GRA proteins interacted with a variety of host cell proteins such as enzymes, structural and functional proteins of organellar proteins of broad spectrum. Several specific bindings of each GRA protein to host proteins were discussed presumptively the role of GRA proteins after secreting into the parasitophorous vacuoles (PV) and the PV membrane in the parasitism of this parasite.

Design and Implementation of a Host Interface for a Regular Expression Processor (정규표현식 프로세서를 위한 호스트 인터페이스 설계 및 구현)

  • Kim, JongHyun;Yun, SangKyun
    • KIISE Transactions on Computing Practices
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    • v.23 no.2
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    • pp.97-103
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    • 2017
  • Many hardware-based regular expression matching architectures have been proposed for high-performance matching. In particular, regular expression processors, which perform pattern matching by treating the regular expressions as the instruction sequence like general purpose processors, have been proposed. After instruction sequence and data are provided in the instruction memory and data memory, respectively, a regular expression processor can perform pattern matching. To use a regular expression processor as a coprocessor, we need the host interface to transfer the instruction and data into the memory of a regular expression processor. In this paper, we design and implement the host interface between a host and a regular expression processor in the DE1-SoC board and the application program interface. We verify the operations of the host interface and a regular expression processor by executing the application programs which perform pattern matching using the application program interface.

Design and implementation of outbound traffic controller for the prevention of ICMP attacks (ICMP 공격 방지를 위한 outbound traffic controller의 설계 및 구현)

  • Yoo, Kwon-jeong;Kim, Eun-gi
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.3
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    • pp.549-557
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    • 2017
  • ICMP(Internet Control Message Protocol) is a main protocol in TCP/IP protocol stack. ICMP compensates the disadvantages of the IP that does not support error reporting. If any transmission problem occurred, a router or receiving host sends ICMP message containing the error cause to sending host. However, in this process, an attacker sends a fake ICMP messages to the host so that the communication can be terminated abnormally. An attacker host can paralyzes system of victim host by sending a large number of messages to the victim host at a high rate of speed. To solve this problem, we have designed and implemented outbound traffic controller that prevents various ICMP attacks. By preventing the transmission of attack messages in different ways according to each case, various network attacks can be prevented. In addition, unnecessary network traffic can be filtered before transmitted.

Multicast Methods in Support of Internet Host Mobility (인터넷 상에서 호스트 이동성을 지원하는 멀티캐스트 방안)

  • Bang, Sang-Won;Jo, Gi-Hwan;Kim, Byeong-Gi
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.5
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    • pp.1231-1242
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    • 1997
  • This paper deals with an IP mukticast protocol in support of host mobility. Most of the previous multicast schemes have utilized an underlying logical strucuture of network topology,in order to provide a certain degree of order and predictability.On the other hand,mobility implies that a host location relaative to the rest of the net-work changes with time;the physical connectivity of the entire network is thus modified as move.In this case.some multicast datagrams nay not delivered properly,or may delivered twice or more,to a mobile host because the destinations will keep moving whlist datagrams are dekivered with different time delay.This paper first describes the relation between host mobility and multicast, by exploring the possible interactions,and presents a multicast scheme in support of Internet host mobility.A revised scheme is then proposed to adapt the multicast semantic and to optimize the communication overhead.

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Geographical Distribution and Host plants of Corythucha marmorata (Uhler) (Hemiptera: Tingidae) in Korea (해바라기방패벌레(Corythucha marmorata (Uhler)) (Hemiptera: Tingidae)의 발생지역 및 기주식물)

  • Kim, Dong Eon;Kil, Jihyon
    • Korean journal of applied entomology
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    • v.53 no.2
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    • pp.185-191
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    • 2014
  • Corythucha marmorata (Uhler) belonging to the family Tingidae (Hemiptera) has been reported in Korea. In 2013, surveys were conducted to study its morphological characteristics, geographical distribution, and host plants. The adult is brown white and 2.77-3.28 mm in length, and the mature nymph is yellowish brown. Heavily infested trees appear yellowish white because they are fed on by the nymphs on the under surface of host plants. C. marmorata were found between early June and late October, and their numbers peaked in early July to late August. In 2013, the occurrence of C. marmorata was confirmed in 27 cities and counties nationwide, and 8 families and 24 species of host plants were identified. C. marmorata feeds on the following major host plants: Conyza canadensis, Aster pilosus, Helianthus annuus, Artemisia princeps, Rudbeckia bicolor, and Solanum melongena.

Study on Electrochemical Detection of Cyclodextrin-molecule Interactions for Sensor Applications (센서 응용을 위한 사이클로덱스트린-분자 상호작용의 전기화학적 검출)

  • Park, Minji;Kim, Sooyeoun;Bae, Joonwon
    • Applied Chemistry for Engineering
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    • v.29 no.5
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    • pp.519-523
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    • 2018
  • Cyclodextrins are a class of oligosaccharides having an extremely low toxicity, so that they have been used for the formation of host-guest complexes and removal of toxic gases or molecules. In this study, the subtle phenomenon associated with the formation of host-guest complexes between cyclodextrin and toxic molecules (methyl paraben) was experimentally investigated. First, the formation of cyclodextrin-methyl paraben complexes was monitored by UV/Vis spectroscopy as a function of the cyclodextrin concentration. Secondly, the electrochemical measurement was performed with the surface engineered Au electrode having cyclodextrin molecules on the Au substrate. The sensing signal derived from the addition of methyl paraben solution into the Au surface was measured delicately. This study can be informative for future applications such as sensors.

Cis-acting Elements in the 3' Region of Potato virus X are Required for Host Protein Binding

  • Kwon, Sun-Jung;Kim, Kook-Hyung;Hemenway Cynthia
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.139-146
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    • 2006
  • The 3' region of Potato virus X (PVX) has the 74 nt 3'-nontranslated region (NTR) that is conserved among all potexviruses and contains several cis-acting elements for minus-strand and plus-strand RNA accumulation. Three stem-loop structures (SL1-SL3), especially formation of SL3 and U-rich sequence of SL2, and near upstream elements in the 3' NTR were previously demonstrated as important cis-acting elements. To Investigate the binding of these cis-acting elements within 3' end with host protein, we used the electrophoretic mobility shift assays (EMSA) and UV-cross linking analysis. The EMSA with cellular extracts from tobacco and RNA transcripts corresponding to the 150 nt of the 3' end of PVX RNA showed that the 3' end of PVX formed complexes with cellular proteins. The specificity of protein binding was confirmed through competition assay by using with 50-fold excess of specific and non-specific probes. We also conducted EMSA with RNAs containing various mutants on those cis-acting elements (${\Delta}10$10, SL3B, SL2A and ${\Delta}21$; J Mol Biol 326, 701-720) required for efficient PVX RNA accumulation. These analyses supported that these cis-acting elements are required for interaction with host protein(s). UV-cross linking analysis revealed that at least three major host proteins of about 28, 32, and 42 kDa in mass bound to these cis-elements. These results indicate that cis-acting elements from 3' end which are important for minus and plus-strand RNA accumulation are also required for host protein binding.

Mineralogy and Chemical Composition of the Residual Soils (Hwangto) from South Korea (우리 나라 황토(풍화토)의 구성광물 및 화학성분)

  • 황진연;장명익;김준식;조원모;안병석;강수원
    • Journal of the Mineralogical Society of Korea
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    • v.13 no.3
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    • pp.147-163
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    • 2000
  • The mineralogy and chemical composition of reddish to brownish yellow residual soils, so called "Hwangto" have been examined according to representative host rocks. The result of the study indicates that Hwangto consists of 40-80% clay minerals and various minerals such as quartz, feldspar, hornblende, goethite, and gibbsite. Clay minerals include kaolinite, halloysite, illite, hydroxy interlayered vermiculite (HIV), mica/vermiculite interstratifield mineral and chlorite. The mineralogical constituents and contents of Hwangto were different depending on the types of host rocks. Moreover, the Jurassic granitic rocks contain relatively more kaolin minerals, whereas the Cretaceous granitic rocks contain more HIV and illite. In addition, reddish Hwangto contains relatively more kaolinite and HIV, and yellowish Hwangto contains more illite and halloysite. It is suggested that feldspars and micas of host rocks were chemically weathered into illite, halloysite, illite/vermiculite interstratified minerals, and HIV, and finally into kaolinite. Compared with their host rocks, the major chemical compositions of Hwangto tend to contain more $Al_2O_3,\;Fe_2O_3,\;H_2O$ in amount and less Ca, Mg, and Na. Hwangto contains relatively high amount of trace elements, P, S, Zr, Sr, Ba, Rb, and Ce including considerable amount of Li, V, Cr, Zn, Co, Ni, Cu, Y, Nb, La, Nd, Pb, Th in excess of 10 ppm. Relatively high amount of most trace elements were detected in the Hwangto. The major and minor chemical compositions of the Hwangto were different depending on the types of host rocks. However, their difference was in the similar range compared with the compositions of host rocks.

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