• 동의생리병리학회지
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• 제19권5호
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• pp.1317-1322
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• 2005

The aim of this study was to find out the effectiveness on germinated seed of Rhynchosia Volubilis for Female Bone Morphogenesis. For this purpose, We compared two methods. water extract and alcohol extract using germinated Rhynchosia Volubilis(GRV) according to germinating days were conducted to measure the polyphosphate contents and investigate HOS-TE85 propagation rate. Both water and alcohol extract two methods were not toxicant. And if not excessively treated, alcohol extract rate were more about $5{\sim}15$ times than water extract rate. So usually water extract were better than alcohol extract. but in case of osteoporosis, alcohol extract were effective.

• 동의생리병리학회지
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• 제18권6호
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• pp.1635-1642
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• 2004

The aim of this study was to find out the effectiveness on germinated Rhynchosia Volubilis for Female Bone Morphogenesis. For this purpose, experiments using germinated Rhynchosia Volubilis(GRV) according to germinating days were conducted to measure the polyphosphate contents and to examine the effects of the transcription activity of gene related to bone morphogenesis on the formation of bone in female. The quantitative analysis of the polyphosphate contents showed that 1 day geminated Rhynchosia Volubilis(GRV) group is treble better contents of polyphosphate than non-germinated Rhynchosia Volubilis, 2 day and 3 day GRV groups. The active of the COL1, OTN, MGP, BMP genes was less than the increase of the polyphosphate contents.

• 대한한의학회지
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• 제23권2호
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• pp.190-197
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• 2002

Objectives : This study was performed to examine the effect of Yukmigiwhang-tang kamibang, a mixture of oriental herbal extracts, on the transcription of bone fonnation genes, BMP4 (bone morphogenetic protein 4) and TG2 (transglutaminase-2). Methods : Bone-related cells, MG-63 (human male osteosarcoma), HOS-TE85 (human female osteosarcoma), and KG-l (bone marrow) were cultured with portions of Yukmigiwhang-tang kamibang and the transcription activities of bone-related genes, BMP4 (bone morphogenetic protein 4) and TG2 (transglutaminase-2), were determined by Reverse-Transcription Polymerase Chain Reaction (RT-PCR). Results : Transcription of BMP4 gene in HOS-TE85 cell increased up to 40% at 0.3% (v/v) of Yukmigiwhang- tang kamibang extract and that of TG2 gene in MG-63 cells also increased up to 40% at 0.3-0.4% of the same extract. Although it was less significant when compared to those in other cells, the transcription of BMP4 gene in KG-l cells also increased up to 10 to 25%. Conclusions : These results clearly demonstrated that Yukmigiwhang-tang kamibang have an effect on transcription activity of bone-related genes, TG2 and BMP4, suggesting that it may play an important role in bone formation.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.233.2-233.2
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• 2003

This experiment study was carried out to prove the efficacy of Cheongpajeon(CPJ) extract in ovariectomized rats. 40 rats were divided into 4 groups, administrated saline after sham operation group(sham-op), administered saline after ovariectomy group(control), administered CPJ 1g/kg after ovariectomy group and administered Livial 0.042mg/kg after ovariectomy group(positive control). We examined the water extract of CPJ that is capable of affecting osteoblast proliferation using MG-63 and HOS-TE85. (omitted)