• Korean Journal of Veterinary Research
• /
• v.61 no.2
• /
• pp.19.1-19.7
• /
• 2021

Feline panleukopenia virus (FPV) causes leukopenia and severe hemorrhagic diarrhea, killing 50% of naturally infected cats. Although intact FPV can serve as an antigen in the hemagglutination inhibition (HI) test, an accidental laboratory-mediated infection is concern. A non-infectious diagnostic reagent is required for the HI test. Here, we expressed the viral protein 2 (VP2) gene of the FPV strain currently prevalent in South Korea in a baculovirus expression system; VP2 protein was identified by an indirect immunofluorescence assay, electron microscopy (EM), Western blotting (WB), and a hemagglutination assay (HA). EM showed that the recombinant VP2 protein self-assembled to form virus-like particles. WB revealed that the recombinant VP2 was 65 kDa in size. The HA activity of the recombinant VP2 protein was very high at 1:2¹⁵. A total of 143 cat serum samples were tested using FPV (HI-FPV test) and the recombinant VP2 protein (HI-VP2 test) as HI antigens. The sensitivity, specificity, and accuracy of the HI-VP2 test were 99.3%, 88.9%, and 99.3%, respectively, compared to the HI-FPV test. The HI-VP2 and HI-FPV results correlated significantly (r = 0.978). Thus, recombinant VP2 can substitute for intact FPV as the serological diagnostic reagent of the HI test for FPV.

• Korean Journal of Veterinary Service
• /
• v.27 no.1
• /
• pp.81-87
• /
• 2004

This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.

• Proceedings of the Korea Concrete Institute Conference
• /
• 2008.11a
• /
• pp.9-12
• /
• 2008

The semi-rigid joint is the shape of middle that can supplement the defect of pin joints and accept the good point of rigid joints. Recently, a study on the pin joints is activated in the country, but because the study on semi-rigid joints is not many, this study tried to prove with producing test model of three shape. The test models are rigid joint HI-R, semi-rigid joint HI-S, pin joint HI-P. As a result of the test, respectively HI-R, HI-S, HI-P appeared shear failure of joint, flexure failure of the top fixing, flexure failure of the lower part slipping stair slab, and the maximum strength is measured to 51.74, 51.4, 24.63kN, the stiffness is appeared 1.58, 1.19, 0.37 respectively, The yield strength is respectively kept 44.5, 47.3, 24kN, and ductility ratio is appeared to 3.31, 2.32, 1.54, when is based on KBC code, sag of the acting service load is appeared that HI-P model is over the standard. When is based on distribution of bars strain ratio, HI-S seems similar behavior incipiently, but after the yield, the semi-rigid joint was able to be judged better than pin joint because of the stress allotment of joint internal elements.

• Korean Journal of Clinical Laboratory Science
• /
• v.56 no.1
• /
• pp.32-42
• /
• 2024

This study compared the results of hemolyzed samples and re-collected samples to investigate a hemolysis influence and an acceptable hemolysis index (HI). Before and after hemolysis, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), amylase (Amy), direct bilirubin (D-bil), total bilirubin (T-bil), creatine phosphokinase (CK), gamma glutamyl transferase (GGT), iron, potassium (K), lactate dehydrogenase (LDH), magnesium (Mg), phosphorus (Phos), total protein (TP), and uric acid (UA) showed significant results in the paired t-test. LDH, K, iron, AST, CK, GGT, TP, Amy and Phos had a high correlation between the degree of hemolysis and the results of samples. When comparing Roche's cut-off HI with HI^QChigh obtained using quality control (QC) high standard deviation (SD), AST, D-bil, CK, and LDH were similar, but Amy, GGT, K, iron, Phos, and TP were lower than the cut-off HI of Roche, while ALP and ALT were higher. Some analytes which showed no significant results in the paired t-test, were found to have significant results in HI>200. Hence, it is suggested that the hemolyzed sample should be rejected if HI>200. Based on this study that some analytes were affected when HI<100, we recommend to set the standard of hemolysis starting from HI>50.

• Korean Journal of Veterinary Service
• /
• v.26 no.3
• /
• pp.215-219
• /
• 2003

This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA) titers and sample to positive ratio (S/P ratio) of Newcastle disease(ND) virus. To perform this study, the 372 sera of broiler chicks and 120 sera of layers and breed chicks were collected from slaughter house and farms, respectively. As a result of HI test out of different chicks, the positive percentage of ND antibody titer of broiler, layer and breeder, when a standard positive HI titer were '2', was 84.4%, 100% and 100%, respectively. The positive percentage of ND antibody titer by ELISA was shown 38.4%, 100% and 100% and S/P ratio were also shown 81.5%, 98.2% and 99.2%, respectively. The results of comparative survey with same sera by two experimental methods were as follows; In low HI titer, ELISA titer was not similar to HI titer, but S/P ratio was similar to it. In high HI titer, ELISA titer was not similar to HI titer. Therefore, HI titer was more similar to S/P ratio than ELISA titer.

• Korean Journal of Veterinary Research
• /
• v.56 no.3
• /
• pp.189-192
• /
• 2016

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

• The Journal of The Korea Institute of Intelligent Transport Systems
• /
• v.9 no.4
• /
• pp.90-101
• /
• 2010

The existing HiPassPlusCard system is composed of the HiPassPlusCard, a Purchase SAM, a Purchase terminal, a settlement system and OBU. However, this system is confronted by the problem with its incompatibility. This problem occurred because the technology companies made cards in their own ways in respect of the parts which were not yet set by KS standard. Thus if existing HiPassPlusCard user wants to use any other public transportations, user would have to buy other cards and have to bring all them. Also if the HiPlusCard wants to extend their business to apply its technology into infrastructure of the other companies, the HiPlusCard has to construct infra independently so that it causes the such problems of duplicated investment. To solve these problems, we have analyzed and upgraded the existing HiPassPlusCard system, and we designed and developed the Nation-wide Interoperable HiPassPlusCard system. Then the conformity test demonstrated that it is efficiency-oriented and technically performs well. Also we, the HiPlusCard, the Korea Financial Telecommunications & Clearings and the KORAIL NETWORKS, run the Test-bed in Gwangju for 6 months. The test-bed indicated that Nation-wide Interoperable HiPassCard system practically works well.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.27 no.10
• /
• pp.623-629
• /
• 2014

For the semiconductor device safety from over current in the digital electronic circuit system must be surely designed that it's surface mount type micro fuse device. In this paper, We has analysed to the fusing character of micro fuse as a function changed thickness of thermosetting ink epoxy. To the change of thermosetting ink epoxy thickness with in production lot, in the electrically character (fusing test in the 2 multiple over current and 10 multiple over current, surface temperature test in the 1.25 multiple over current) of micro fuse has been tested. According to the electrically character result, changed thickness of thermosetting ink epoxy in designed micro fuse withheld direct effect in both end resistance changes. Also, because high thermal energy in the micro fuse test of over current was occurred to effect such as thermal runaway and explosion. Therefore, screen printing process in the design of micro fuse using thermosetting ink epoxy is very important for production quality improvement.

• Journal of the Korean Geotechnical Society
• /
• v.22 no.4
• /
• pp.11-19
• /
• 2006

For the last decade, the hi-directional testing method has been advantageous over the conventional pile load testing method in many aspects. However, because the hi-directional test uses a loading mechanism entirely different from that of the conventional pile load testing method, many investigators and practicing engineers have been concerned that the hi-directional test would give inaccurate results, especially about the pile head settlement behavior. Therefore, a hi-directional load test and the conventional top-down load test were executed on 1.5 m diameter cast-in-situ concrete piles at the same time and site. Strain gauges were placed on the piles. The two tests gave similar load transfer curves at various depth of piles. However, the top-down equivalent curve constructed from the hi-directional load test results predicted the pile head settlement under the pile design load to be about one half of that predicted by the conventional top-down load test. To improve the prediction accuracy of the top-down equivalent curve, a simple method that accounts for the pile compression is proposed. It was also shown that the strain gauge measurement data from the hi-directional load test could reproduce almost the same top-down curve.

• Journal of Power System Engineering
• /
• v.17 no.4
• /
• pp.109-114
• /
• 2013

In this study, the microstructure, mechanical properties and high temperature oxidation characteristics of HiSiMo and HiSiMoM ductile iron for exhaust manifold were investigated. The HiSiMoM ductile iron was developed by optimization of alloying element addition and casting design. The exhaust manifold prototype was fabricated using the HiSiMoM iron and this resulted in the weight saving of 0.73kg. The microstructures of the HiSiMo and HiSiMoM irons were similar each other and graphite nodularity was 89% and 93% respectively. Tensile strengths of them were 663.5 and 674.4 MPa and Brinell hardness were 235.3 and 243.9 respectively. Both irons showed parabolic weight gain behavior in high temperature oxidation atmosphere. Oxidation layer was divided into external and internal layers. The weight gain of the HiSiMoM iron was lower than that of the HiSiMo iron after isothermal oxidation test at $900^{\circ}C$. This should be rationalized by higher Si enrichment at the interface of the matrix and internal layer of the HiSiMoM iron.