• 농약과학회지
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• 제9권3호
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• pp.191-198
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• 2005

신규 살충제 표적 단백질인 AnCE의 활성부위 잔기들과 상호작용 가능한 약리단 (pharmacophore)을 세 개의 펩타이드로 이루어진 ACE 기질 Hippuryl-L -histidyl-L-leucine (Hip-L-His-L-Leu, HHL) 분자의 구조를 모델로 하여 예측하였다. HHL의 분자구조, 용액장 내에서의 구조변화 그리고 약리단을 구성하는 원자들의 전하밀도 분석을 위해 순이론적인 양자화학 계산방법을 이용하여 구조 최적화, NMR 화학적 이동 및 NPA 계산을 수행하였다. 이론적인 NMR 화학적 이동 값들은 실험 결과와 잘 일치함을 보였고 전하밀도 계산 결과는 해당원자의 약리단을 분석하는데 사용되었다. 결과적으로 HHL 분자 구조를 통해 소수성(aromatic, aliphatic), 수소결합 주게, 수소결합 받게, 금속 아연 결합부위의 5개 약리단을 추출할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.952-959
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• 2007

His-His-Leu (HHL), a tripeptide derived from a Korean soybean paste, is an angiotensin-I-converting enzyme (ACE) inhibitor. We report here a method of producing this tripeptide efficiently by expressing tandem multimers of the codons encoding the peptide in E. coli and purifying the HHL after hydrolysis of the peptide multimers. The HHL gene, tandemly multimerized to a 40-mer, was ligated with ubiquitin as a fusion gene (UH40). UH40 was inserted into vector pET29b; the UH40 fusion protein was then produced in E. coli BL21. The recombinant UH40 protein was purified by cation-exchange chromatography with a yield of 17.3mg/l and analyzed by matrixassisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry and protein N-terminal sequencing. Leucine aminopeptidase was used to cleave a 405-Da HHL monomer from the UH40 fusion protein and the peptide was purified using reverse-phase high-performance liquid chromatography (HPLC) on a C18 HPLC column, with a final yield of 6.2mg/l. The resulting peptide was confirmed to be HHL with the aid of MALDI-TOF mass spectrometry, glutamine-TOF mass spectrometry, N-terminal sequencing, and measurement of ACE inhibiting activity. These results suggest that our production method is useful for obtaining a large quantity of recombinant HHL for functional antihypertensive peptide studies.

• 한국자기공명학회논문지
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• 제10권1호
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• pp.115-125
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• 2006

Hippuryl-L-histidyl-L-leucine(HHL) is widely used as a substrate of angiotensin converting enzyme(ACE) cleaving the neurotransmitter angiotensin(I) to the octapeptide angiotensin(II). The structure of the substrate molecules should provide information regarding the geometric requirements of the ACE active site. For the purpose of determination of in vivo reaction, metallo(Cu, Zn)-HHL complexes were synthesized and the degree of complex formation were identified by MALDITOF, ESI mass spectrometric analysis. Tn addition, the pH-dependent species distribution curves were obtained by potentiometric titration. Nitrogen atoms of imidazole ring and oxygen atom of caboxylate groups in the peptide chain were observed to be participated in the metal complex formation. After purification of complexes further structural characterization were made by utilizing UV-Vis, electrochemical methods and NMR. Complete NMR signal assignments were carried out by using 2D-spectrum techniques COSY, TOCSY, NOESY, HETCOR. A complex that two imidazole and carboxylate groups are asymmetrically participating to coordination mode was predicted to the solution-state structure of $Cu(II)-HHL_2$ based on $^{13}C-NMR$ signal assignment and NOE information.

• 말소리와 음성과학
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• 제7권1호
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• pp.107-116
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• 2015

The present experiment investigated the effect of word length on the length of f0 intervals for North Kyungsang children. In order to find out the lengths of the f0 intervals, the f0 values at the midpoints of vowels in words were measured. F0 estimates were computed as intervals consistent with the logarithmic scale corresponding to the number of syllables in the words. The results indicated that the mean f0 intervals in words of different lengths showed a significant difference for the HH in HH vs. HHL and the LH in LH vs. LLH for North Kyungsang children. Adult speakers from the North Kyungsang region significantly differed only within the HH in HH vs. HHL. Adult speakers made a noticeable contribution in this characteristic from the children. The result of the adult study was presented to confirm whether the children used a North Kyungsang dialect. With respect to individual speaker differences, the North Kyungsang children showed more or less consistent patterns in quantile-quantile plots for the HH vs. HHL, but for the HL vs. LHL and LH vs. LLH, there were more variations than for the HH vs. HHL. The individual speakers' variation was the largest for the HL vs. LHL and the smallest for HH vs. HHL. Considering these results, the effect of word length on f0 intervals tended to show pitch accent-type-specific characteristics in the process of prosodic acquisition.

• 한국결정성장학회지
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• 제12권6호
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• pp.269-269
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• 2002

희박 자성 반도체 ZnMnTe를 장벽층으로 사용한 양질의 ZnTe/ZnMnTe 단일 양자우물 구조를 열벽적층 성장법으로 성장하였다. 고분해능 X-선 회절측정 결과 ZnTe 우물층이 강하게 스트레인을 받고 있음을 알 수 있었다. 광발광 측정으로부터 무거운 양공 엑시톤 (el-hhl)과 가벼운 양공 엑시톤 (el-lhl)의 매우 뾰족한 발광 크들이 나타남을 관측하였다. 또한 우물층의 두께가 증가함에 따라 (el-hhl)과 (el-lhl)의 엑시톤 관련 피크들은 낮은 에너지 쪽으로 이동하였다. 광발광 피크 세기의 온도에 따른 변화는 운반자들의 열적 활성화로 설명할 수 있었다.

• 한국결정성장학회지
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• 제12권6호
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• pp.267-271
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• 2002

희박 자성 반도체 ZnMnTe를 장벽층으로 사용한 양질의 ZnTe/ZnMnTe 단일 양자우물 구조를 열벽적층 성장법으로 성장하였다. 고분해능 X-선 회절측정 결과 ZnTe 우물층이 강하게 스트레인을 받고 있음을 알 수 있었다. 광발광 측정으로부터 무거운 양공 엑시톤 (el-hhl)과 가벼운 양공 엑시톤 (el-lhl)의 매우 뾰족한 발광 크들이 나타남을 관측하였다. 또한 우물층의 두께가 증가함에 따라 (el-hhl)과 (el-lhl)의 엑시톤 관련 피크들은 낮은 에너지 쪽으로 이동하였다. 광발광 피크 세기의 온도에 따른 변화는 운반자들의 열적 활성화로 설명할 수 있었다.

• 음성과학
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• 제15권2호
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• pp.21-30
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• 2008

The current study investigated distributional properties of the Korean Accentual Phrase and their implication to word segmentation. The properties examined were the frequency of various AP tonal patterns, the types of tonal patterns that are imposed upon content words, and the average number and temporal location of content words within the AP. A total of 414 sentences from the Read speech corpus and the Radio corpus were used for the data analysis. The results showed that the 84% of the APs contained one content word, and that almost 90% of the content words are located in AP-initial position. When the AP-initial onset was not an aspirated or tense consonant, the most common AP patterns were LH, LHH, and LHLH (78%), and 88% of the multisyllabic content words start with a rising tone in AP-initial position. When the AP-initial onset was an aspirated or tense consonant, the most common AP patterns were HH, HHLH, and HHL (72%), and 74% of the multisyllabic content words start with a level H tone in AP-initial position. The data further showed that 84.1% of APs end with the final H tone. The findings provide valuable information about the prosodic pattern and structure of Korean APs, and account for the results of a previous study which showed that Korean listeners are sensitive to AP-initial rising and AP-final high tones (Kim, 2007). This is in line with other cross-linguistic research which has revealed the correlation between prosodic probability and speech processing strategy.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1688-1694
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• 2009

A novel strain of fluorescent pseudomonad (PB-St2) was isolated from surface-sterilized stems of sugarcane grown in Pakistan. The bacterium was identified as Pseudomonas aurantiaca on the basis of 16S rRNA gene sequence analysis and results from physiological and biochemical characteristics carried out with API50 CH and QTS 24 bacterial identification kits. Assays using substrate-specific media for enzymes revealed lipase and protease activities but cellulase, chitinase, or pectinase were not detected. The bacterium was unable to solubilize phosphate or produce indole acetic acid. However, it did produce HCN, siderophores, and homoserine lactones. In dual culture assays on agar, the bacterium showed antifungal activity against an important pathogen of sugarcane in Pakistan, namely Colletotrichum falcatum, as well as for pathogenic isolates of Fusarium oxysporium and F. lateritium but not against F. solani. The antifungal metabolites were identified using thin-layer chromatography, UV spectra, and MALDI-TOFF spectra and shown to be phenazine-1-carboxylic acid (PCA), 2-hydroxyphenazine (2-OH-PHZ), and N-hexanoyl homoserine lactone (HHL) (assessed using only TLC data). The capacity of this bacterium to produce HCN and 2-OH-PHZ, as well as to inhibit the growth of C. falcatum, has not been previously reported.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.263-270
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• 1998

The purpose of this study was to investigate the effect of Lycii fructus on the serum lipid in rats fed high fat diet. We compared the effects of L. fructus and L.fructus water extract both adminstered with high fat diets on rats that had previously been on high fat or standard diets. Two separate experiments were conducted for 6 weeks. respectively. In experiment I, 4 groups of rats were fed experimental diets consisting of either \circled1 6 weeks of a standard diet(control), \circled2 6 weeks of a high-fat diet(HHC), \circled3 3 weeks of a high-fat diet followed by 3 weeks of a high-fat diet containing L. fructus(HHL) or \circled4 6 weeks of a high-fat diet with L. fructus extract in place of water for the last 3 weeks (HHT). In the second set of experiments, a high-fat diet (SHC), high-fat diet containing L.fructus(SHL) or high-fat diet with L. fructus extract in place of water (SHT) were fed for 3 weeks after 3 weeks of standard diet feeding. Rats fed L. fructus diet consumed more diets than high-fat diets. THe results of experiment I showed significant decreases(p<0.05) in serum triglyceride(TB) and low density lipoprotein-cholesterol (LDL-C) levels with L. fructus feedings, but did not show andy changes in total cholesterol (TC) level. High density lipoprotein-cholesterol (HDL-C) level was increased significantly(p<0.05) with L. fructus feedings. Therefore, the ration of LDL-C to HDL-C(LDL-C/HDL-C) which is used as an atherosclerosis index was significantly (p<0.05) low, while the HDL-c/TC ration was significantly(p<0.05) high with L.fructus intake. However, no significant were found in serum cholesterols and TG levels in experimentII. The results of these experiments indicate that , regardless of the feeding from, L. fructus can be beneficial in lowering serum TG and LDL-C levels for habitual high-fat diet intakers. L.fructus also seems to be effective in elevating serum HDL-C level, theregy having beneficial effects on atherosclerosis by influencing the serum lipoprotein profile.

• 한국산업정보학회논문지
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• 제14권2호
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• pp.72-80
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• 2009

본 논문에서는 무선 센서 노드에 사용 가능한 저가형 플래시 메모리를 위한 하드웨어 추상화 구조(Hardware Abstraction Architecture: HAA)를 제안한다. 제안된 HAA는 3개 의 계층으로 이루어져 있으며, 세 개의 계층은 HIL(Hardware Interlace Layer), HAL(Hardware Adaption Layer), HPL(Hardware Presentation Layer)로 구성된다. 여기서 HIL은 상위 계층의 어플리케이션에 대해 플랫폼 독립적인 인터페이스를 제공하고, HAL은 하드웨어 추상계층에서 가장 핵심적인 부분으로서 하드웨어 자원 제어, 상태관리,논리적 명령어를 생성하며, HPL은 하드웨어 초기화 및 플래시 메모리와의 통신 부분을 담당한다. 제안된 HAA는 무선 센서노드에 가장 많이 사용되고 있는 Atmel사의 AT45DB 계열의 플래시 메모리에 적용되었으며, 4,384 바이트의 프로그램 메모리와 195 바이트의 데이터 메모리를 사용한다. 따라서 본 논문에서 제안된 HAA 구조는 3계층으로 설계되었기 때문에 소프트왜어 개발 측면에서 높은 유연성, 확장성, 재사용성을 제공하며, 낮은 메모리를 시용하기 때문에 무선 센서 노드용으로 적합하다 할 수 있다.