• 생명과학회지
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• 제8권3호
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• pp.279-284
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• 1998

한국인 환자의 혈청에서 분리한 HGV 5'-UTR영역의 염기서열을 결정하였다. 이들 염기서열을 이미 보고된 서열들과 비교한 결과, 한국 분리주들은 일본 분리주들과 더 높은 상동성을 나타내어 지리적 격리에 의해 HGV의 염기서열의 변이가 축적되었음을 알 수 있다. 흥미롭게도 동일 지역에서 분리된 HGV 분리주들 간에는 고도로 보존되어 있어 HGV의 분류에 이용가능한 세 개의 영역이 5'-UTR에서 발견되었다. 이들 그룹-특이적 영역에 기초하여, 24 HGV 분리주들을 5개의 그룹으로 분류할 수 있었다.

• 생명과학회지
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• 제8권4호
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• pp.360-365
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• 1998

The genetic of a recently described virus, hepatitis G virus(HGV) was investigated. HGV envelope 1 (E1) nucleotide sequences isolated from six Korean hepatitis b virus-positive patients by using a reverse transcription-poly-merase chain reaction procedure, were analysed and compared to the seven previously reported HGV isolates. Sequence homology among the Korean isolates was 88-97% whereas among the isolates from different geographic areas was 80-92%, indicating geographical divergence of HGV. Nucleotide substitutions spread uniformly throughiut the E1 fragment. Furthermore, compared to the prototype HGV sequence, frameshift mutations were observed in most of the Korean isolating that a different translating initiation site for the polyprotein exists in the Korean type HGV.

• 한국식품과학회지
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• 제46권6호
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• pp.743-749
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• 2014

본 연구에서는 수경재배 인삼의 전초를 이용하여 식초를 제조하여 진세노사이드의 패턴 및 함량 변화를 확인하고자 하였으며, 고지방 식이로 유도된 비만 쥐에 수경재배 인삼 식초를 투여하여 체중, 체지방 및 지질대사를 분석함으로써 항비만 식품으로서 가능성을 확인하고자 하였다. 실험 결과 수경재배 인삼으로 식초 제조 시 산도는 $5.60{\pm}0.24%$이었으며, 초산 발효 과정에서 진세노사이드 Re가 감소하고 진세노사이드 Rg2가 약 4배 수준 증가하는 것으로 확인되었다. 수경재배 인삼 식초의 항비만 효과를 확인하기 위해 4주령 C57BL/6J mice에 고지방 식이를 투여하면서 수경재배 인삼 식초 및 녹차추출물을 8주간 투여하였다. 실험식이 종료 후 체중 변화를 비교한 결과 수경재배 인삼초투여군(HGV) 및 녹차추출물투여군(GT)은 고지방식이군(HFD)과 비교하여 유의적인 체중감소효과가 관찰되었다. 체성분 분석결과 복부지방 및 피하지방의 함량이 낮은 것을 확인할 수 있었으며, 개복 후 복부 주위의 지방을 적출하여 무게 측정 시 고지방식이군(HFD)과 비교하여 수경재배 인삼초투여군(HGT) 및 녹차추출물 투여군(GT)은 각각 85.5, 83.3% 낮아진 것으로 확인되었다. 혈중지질프로파일 분석 결과 수경재배 인삼초투여군(HGV) 및 녹차추출물투여군(GT)은 중성지방, 총콜레스테롤, LDL 콜레스테롤이 고지방식이군(HFD)과 비교하여 유의적으로 낮아졌으며, HDL 콜레스테롤이 증가된 것으로 확인되었다. 고지방 식이에 의한 간 독성을 관찰하기 위해 AST 및 ALT 효소 활성을 측정하였으며, 수경재배 인삼초투여군(HGV)은 고지방식이군(HFD)과 비교하여 각각 67.3, 75.1%, 녹차추출물투여군(GT)은 각각 68.4, 75.1% 감소 효과가 관찰되었다. 이와 같은 결과를 통해 수경재배 인삼으로 식초 제조 시 흡수율이 우수한 proginseonside Rg2의 함량이 증가되어 생리활성 강화 효과를 기대할 수 있으며, 체지방 축적을 억제하고 혈청 지질 조성을 개선시킴으로써 항비만 식품소재로 가치가 있을 것으로 생각된다.

• Computers and Concrete
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• 제22권6호
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• pp.515-525
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• 2018

This paper presents a numerical study on the performance of reinforced concrete (RC) bridge structures subjected to heavy goods vehicle (HGV) collision. The objectives of this study are to investigate the dynamic response and failure modes of different types of bridges under impact loading as well as to give an insight into the simplified methods for modeling bridge structures. For this purpose, detailed finite-element models of HGV and bridges are established and verified against the full-scale collision experiment and a recent traffic accident. An intensive parametric study with the consideration of vehicle weight, vehicle velocity, structural type, simplified methods for modeling bridges is conducted; then the failure mode, impact force, deformation and internal force distribution of the validated bridge models are discussed. It is observed that the structural type has a significant effect on the force-transferring mechanism, failure mode and dynamic response of bridge structures, thus it should be considered in the anti-impact design of bridge structures. The impact force of HGV is mainly determined by the impact weight, impact velocity and contact interface, rather than the simplification of the superstructure. Furthermore, to reduce the modeling and computing cost, it is suggested to utilize the simplified bridge model considering the inertial effect of the superstructure to evaluate the structural impact behavior within a reasonable precision range.

• 대한바이러스학회지
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• 제28권2호
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• pp.175-182
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• 1998

We investigated the rate of hepatitis G virus infection among 50 patients who were not infected with the hepatitis C virus but showed symptoms of hepatitis. Viral RNA was extracted from the patients' sera and cDNA was synthesized and amplified by RT-PCR (reverse transcription-polymerase chain reaction) using random hexamer and 5 primers (470-20-1-77F, 470-20-1-211R, 470-20-1-211R-biotin, GV57-4512MF, GV57-4657MR). The amplified PCR products were confirmed by electrochemiluminescence (ECL), liquid hybridization (LH) and Southern blotting (SB). Among the 50 PCR products, by means of ECL, we found 4 samples to be positive and 5 samples to be indeterminate. The GV45-89M probe (5'-CYCGCTGRTITGGGGTGTACfGGAAGGC-3') was end-labelled with gamma-$^{32}P$ ATP and used for liquid hybridization with the PCR products. By using liquid hybridization, we detected specific bands from 4 positive sera and also from one indeterminate serum as determined by ECL. An 1.5% agarose gel electrophoresis of the 9 PCR products which were HGV positive or indeterminate as determined by ECL showed a 160bp band from 4 positive and one indeterminate serum. The 5 PCR products proved to be positive when SB was applied with the GV45-89M probe as well as when LH was applied. LH and SB were shown to have higher sensitivity and specificity than ECL. Two cases among 5 positive cases had relatively high SGOT, SGPT, ALP values when compared with other 48 cases. In summary, we confirmed hepatitis G virus infection in 5 cases among 50 Korean patients showing symptoms of viral hepatitis.

• 한국안전학회지
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• 제20권3호
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• pp.112-119
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• 2005

In road tunnel, in order to prevents an accident and minimize the damage of an accident in the case of fire, safety facilities and equipments are integral parts. The type and amount of safety facilities are based on tunnel type and length, traffic flow rate, etc. Therefore many countries use a tunnel classification system that categories tunnel into groups, and specifies the necessary emergency equipment for each group. In this study, for the purpose of classifying tunnel based on tunnel ist investigated the domestic and foreign standards and regulations for safety of road tunnel. As a results, we suggest the method of classification of tunnel by traffic performance, tunnel grade, the volume of traffic, fraction of HGV, rules or regulations for transports of dangerous good through tunnel.

• 한국항공우주학회지
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• 제48권9호
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• pp.731-743
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• 2020

극초음속 활공 비행체는 로켓 부스터에 의해 높은 고도로 올라가서 부스터에서 분리된 후 대기권 내에서 진행 방향을 바꾸면서 약 30~70km 고도에서 마하 5 이상의 극초음속으로 활공한다. 이는 포물선 궤적이 아닌 예측 불가능한 비행경로로 이동하므로 현재 미사일 방어 체계로 요격이 어렵다. 미국은 2010년대 초에 HTV-2와 AHW 비행시험을 수행하여 극초음속 활공 비행 가능성을 확인했고, 최근에 LRHW, ARRW 등 극초음속 활공 비행체 시스템을 개발하고 있다. 중국은 DF-ZF (WU-14) 활공 비행체를 2014년부터 비행시험을 수차례 수행했고 DF-17 미사일에 탑재하여 운용하고 있다. 러시아는 구소련 시절부터 극초음속 활공 비행체 연구를 수행했지만 실패를 거듭했고 근래에 Avangard (Yu-71) 활공 비행체를 SS-19 ICBM에 탑재하여 비행시험에 성공하였다. 본 논문에서는 미국, 중국, 러시아, 일본, 인도, 유럽에서 개발했거나 현재 개발 중인 극초음속 활공 비행체의 특성, 비행시험 사례 및 개발 동향을 고찰하였다.

• 대한바이러스학회지
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• 제29권1호
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• pp.45-53
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• 1999

We examined the hepatitis G virus infections among 227 Koreans who were healthy or were suspected of hepatitis and determined the phylogenetic relationship based on a part of the NS-5 region of 5 positive samples. Viral RNA was extracted from sera and cDNA was synthesized and subsequently amplified by RT-PCR (reverse transcription-polymerase chain reaction) or RT-nested PCR using random hexamer and NS-5 specific primers (470-20-1-77F, 470-20-1-211R, HGVNESTFO, HGVNESTRE). Five positives were found to belong to samples of patients showing symptoms of viral hepatitis. Primers used for PCR or nested PCR were derived from the NS-5 region. On the other hand, no amplification was detected using primers derived from the 5'-NCR (G-146F, G-401R). We performed TA cloning and sequencing of 5 amplified fragments, and their sequences were compared with those of foreign isolates of HGV. The phylogenetic analysis using MegAlign programme of DNAstar has shown that the Korean isolates are clustered on the phylogenetic tree. In summary, we confirmed the hepatitis G virus infection in 5 cases out of 12 patients showing the symptoms of viral hepatitis. The phylogenetic analysis of sequences of 5 amplified fragments showed that their relations to each other were closer than those to the foreign HGV isolates reported.

• 한국군사과학기술학회지
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• 제27권2호
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• pp.285-293
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• 2024

This paper describes the interception using fine fragments and particles to hypersonic vehicles which have a vulnerability in thermal and pressure during glide-phase flight. This interception concept is based on the fast relative velocity and the flight vulnerability of hypersonic vehicles. For the density calculation of fragmentation and particle in interception, error analysis of end-phase was performed including radar, intercept missile and target maneuvering errors. In relation to the vulnerability and error analysis, the penetration characteristics of fine fragments in high temperature were analyzed. Presented the interception in glide-phase could be applied to the concept of horizontal multi-layer defense to hypersonic vehicles.

• 한국도로학회논문집
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• 제19권6호
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• pp.13-21
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• 2017

PURPOSES : In this research, an SB3-level roadside barrier for a highway transition zone that meets the newly established guide Installation and Management Guide for Roadside Safety Appurtenance is developed. Its performance is evaluated by a numerical simulation and real-scale vehicle impact test. METHODS : The commercial explicit dynamic software LS-DYNA is utilized for impact simulation. An FE model of a passenger vehicle developed and released by the National Crash Analysis Center (NCAC) at George Washington University and a heavy goods vehicle (HGV) model developed by the TC226/CM-E Work Group are utilized for impact simulation. The original vehicle models were modified to reflect the conditions of test vehicles. The impact positions of the passenger vehicle and truck to the transition guardrail were set as 1/2 and 3/4 of the transition region, respectively, according to the guide. RESULTS : Based on the numerical simulation results of the existing transition barrier, a new structural system with improved performance was suggested. According to the result of a numerical simulation of the suggested structural system, two sets of transition barriers were manufactured and installed for real-scale vehicle impact tests. The tests were performed at a test field for roadside safety hardware of the Korea Highway Corporation Research Institute. CONCLUSIONS : The results of both the real-vehicle impact tests and numerical simulations of the developed transition barrier satisfied the performance criteria, and the results of numerical simulation showed good correlation with the test results.