• 동의생리병리학회지
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• 제22권5호
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• pp.1250-1255
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• 2008

Onchungeum(OCE), a herbal formula, has been used for treatment of anemia, discharging blood and skin diseases. In the previous study, we investigated the anti-cancer effect of OCE by G1 arrest of the cell cycle in human hepatocarcinoma cells, Hep3B cells. In this study, it was examined that the difference of anti-proliferative mechanisms by change in the ratio of OCE composition (OCE I) in Hep3B cells. Treatment of OCE I exhibited a relatively strong anti-proliferative activity and caused various morphological changes such as membrane shrinkage and cell floating. In addition, OCE-I arrests the cell cycle at G1 phase, which was associated with the down-regulation of cyclin D1 and Cdk6 expressions. The G1 arrest was also associated with the induction of Cdk inhibitors p27 and p21. Moreover, both p21 and p27 were detected by immunoprecipitation with anti-Cdk4 and anti-Cdk2 antibodies in OCE I-treated cells but in case of OCE, p21 did not make any complexes with Cdk4 and Cdk2. These results suggest that the change in the ratio of OCE composition might induce different mechanisms in anti-cancer efficacy of OCE, which may confer characteristic principles in oriental medical formula.

• 생약학회지
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• 제39권1호
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• pp.68-73
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• 2008

The present study represents the screening of the protective effects of herbal methanolic extracts from Baekdu mountain against tacrine-induced cytotoxicity in HepG2 cells. Tacrine is an acetylcholinesterase inhibitor, and used for the treatment of Alzheimer's disease. However, administration of tacrine for the treatment of Alzheimer's disease results in a reversible hepatotoxicity in 30-50% of patients, consequently limiting clinical use. Therefore, studies for natural products with protective effect on the tacrine-induced hepatotoxicity would be valuable as providing potential therapeutic use. 109 plant sources were collected in Baekdu mountain, and extracted with methanol. These extracts had been screened the protective effects against tacrine-induced cytotoxicity in HepG2 cells at the 100 and 300 ${\mu}g/ml$. Of these, ten methanolic extracts, roots of Ampelosis japonica, aerial parts of Berberis amurensis, aerial parts of Sedum aizoon, aerial parts of Lespedeza tomentosa, aerial parts of Lespedeza juncea, aerial parts of Hypenricum ascyron, stem barks of Syringa reticulata, fruits of Gleditsia japonica, aerial parts of Chamaenerion angustifolium, branches of Ginkgo biloba, showed significant protective effects against tacrine-induced cytotoxicity in HepG2 cells.

• 동의생리병리학회지
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• 제19권6호
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• pp.1594-1598
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• 2005

Scopoletin (6-methoxy-7-hydrorycournarin) is a phenolic coumarin and a member of the phytoalexins. In this study we investigated whether scopoletin causes apoptosis in human hepatoma HepG2 cells and, if so, by what mechanisms. We report that scopoletin induced apoptosis as confirmed by a chromatin condensation. The signal cascade acivated by scopoletin included the activation of caspase-3 as evidenced by increased pretense activity. Activation of caspase-3 resulted in the cleavage of 116 kDa poly(ADP-ribose) polymerase (PARP) to 85 kDa cleavage product in a dose-dependent fashion. Also, scopoletin-induced apoptotic mechanism of HepG2 cells involved the generation of hydrogen peroxide. Taken together, these results suggest that scopgletin induces hydrogen peroxide generation, which, in turn, causes activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death in HepG2 cells.

• Applied Biological Chemistry
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• 제40권6호
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• pp.577-582
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• 1997

감귤류에 포함되어있는 성분중, 생리활성물질로 거론되어지고 있는 citrus flavonoid 즉, hesperidin, hesperetin, naringin 및 naringenin 4종류를 가지고 간장triglyceride합성의 율속효소로 알려져 있는 phosphatidate phosphohydrolase 활성에 대하여 in vitro계에 미치는 영향과 이들 flavonoid가 HepG2 세포의 생존율 및 증식에 미치는 영향에 대해서도 함께 검토하였다. 그 결과 본 실험에 사용된 모든 flavonoid 는 $10{-4}M$ 농도까지는 세포생존율 및 증식이 최고에 도달하였으나, $10{-3}M$ 농도에서 aglycone flavonoid는 현저한 저해효과를 나타냈다. naringin 및 hesperidin의 glycoside flavonoid는 첨가한 모든 농도에서 PAP활성을 촉진시키는 경향을 나타내었다. 그러나, aglycone flavonoid인 hesperetin은 PAP활성을 농도 의존적으로 저해하였으며, naringenin은 $10{-2}M$ 농도에서 강한 저해효과를 나타내었다. 이러한 결과로 미루어 볼 때 aglycone flavonoid가 생체내에서 세포 생존율, 증식 및 지질대사에 강한 영향을 미칠 것으로 생각되어진다.

• 한국식품과학회지
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• 제44권3호
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• pp.362-366
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• 2012

수산물 추출물로부터 간세포내 지질축적억제효과 추출물의 탐색을 통해 지방간 형성 억제 활성물질을 밝힐 목적으로, 각 48종의 수산물 추출물의 사람 간암세포유래인 HepG2 세포주에서의 중성지질축적억제 효과를 조사하였다. 그 결과, 18개의 추출물에서 유의적인 지질축적억제효과를 보였고, 그 중 Pagrus major, Octopus minor, Enteroctopus dofleini, Undaria pinnatifida Sporophyll, Styela clava, Enteromorpha intenstinalis 등 6개의 추출물이 유효한 효과가 있음을 관찰하였다. 이상의 결과를 바탕으로 지질대사에 관여하는 단백질들의 발현 정도를 조사하고, 활성물질의 분리 및 정제 등에 관한 연구와 지방간 형성 억제 효과에 대한 기전 연구가 진행되어야 할 것으로 사료된다.

• 한국식품과학회지
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• 제43권4호
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• pp.432-437
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• 2011

발효기간에 따른 미나리 발효추출액의 품질 특성을 검토하고 간암세포주에 대한 증식 억제 효과를 알아보았다. 발효 숙성 기간 중에 총 플라보노이드 함량, 색도 및 생균수는 감소하였으며, 포도당과 과당과 같은 환원당 함량은 증가하였다. 정상 간세포주와 간암 세포주를 이용하여 농도별로 시료 처리를 한 후 각 세포의 증식에 대한 효과를 알아 본 결과, 미나리 발효액이 정상 간세포의 증식에는 영향을 미치지 않았으며, 인간유래 간암 세포주인 HepG2의 증식을 농도 의존적으로 억제하였다. 특히 미나리발효액 1년에서 현저한 억제 효과를 확인하였다. 본 연구 결과는 미나리 발효액의 발효 기간에 따른 품질의 변화 및 간암세포 성장 억제 효능을 확인함으로서, 천연의 미나리 발효 추출액을 다양한 식품 산업에 활용하기 위한 기초적 데이터를 처음으로 제시했다.

• 한국식품영양학회지
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• 제27권3호
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• pp.400-405
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• 2014

최근 알로에 베라 섭취에 의한 독성 간염이 보고되고 있으나, 아직까지 알로에 베라의 간에 대한 염증 효과가 명확히 밝혀지지 않았다. 본 연구는 알로에 베라 에탄올 추출물이 간세포의 염증 발현에 미치는 영향과 그 기전을 밝히기 위해 수행되었다. $0.001{\sim}100{\mu}g/mL$의 알로에 베라 추출물을 HepG2 세포에 처리하여 MTT assay를 시행한 결과, 모든 농도에서 세포사멸이 유도되지 않았다. 그러나 모든 농도에서 알로에 베라 추출물은 염증성 사이토카인인 IL-8의 분비를 15.7~25.8%까지 유의적으로 증가시켰다(p<0.05). 또 다른 사이토카인인 M-CSF도 알로에 베라 추출물에 의해 36.3~61.5%까지 유의적으로 분비가 증가되었다(p<0.05). 본 연구 결과는 알로에 베라 추출물이 IL-8과 M-CSF와 같은 염증성 사이토카인 분비기전에 의해 간에 염증을 유발할 수 있음을 보여준다. 또한 알로에 추출물에 의해 유발될 수 있는 간염기전을 제시함으로써, 향후 수행될 추가 실험을 위한 기초 자료로 그 가치가 높을 것으로 사료된다.

• 대한한의학회지
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• 제26권1호
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• pp.46-58
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• 2005

Objective : This study on Sipimikwanjung-tang was undertaken to evaluate its antioxidant capacities and antiperoxidation activities in rat liver tissues. Sipimikwanjung-tang which has been one of the prescriptions in sasang constitutional medicine is usually applied for the therapy of various liver diseases. It is elucidated that Sipimikwanjung-tang has antioxidants on liver tissue of rat and the cytotoxic effects on human hepatoblastoma Hep G2 cells. Methods: Sipimikwanjung-tang extract in antioxidant effects of Hep G2 cells is evaluated by MTT assay, DAPI staining, DNA fragmentation assays and FACS can analysis. Results: Sipimikwanjung-tang induced apoptosis in Hep G2 cells, and induced G1 and G2M arrest of the cell cycle as well as a significant increase in PARP and caspase-3 activity. It induced an increase in $H_2O_2$ generation and the subsequent $NF-{\kappa}B$ activation and also induced cell apoptosis through the caspase-3-dependent pathways in the low concentration of Sipimikwanjung-tang extracts. However, the high dose of Sipimikwanjung-tang extract in Hep G2 cells inhibited $TGF-{\beta}l-induced$ apoptosis via increase in cellular $H_2O_2$, formation and $NF-{\kappa}B$ activation in human hepatoblastoma Hep G2 cells. Conclusion: From this study, the possibility that Sipimikwanjung-tang extracts apply to antioxidant and apoptotic treatment of disease is revealed.

• Journal of Ginseng Research
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• 제20권3호
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• pp.241-247
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• 1996

The effects of ginseng total saponin, ginsenoside-Rb, and -Rb, on the reduction of chmlesterol level and the myNA expression rates of HMG CoA reductase and LDL receptor in Hep G2 were investigated and compared with that of lovastatin, a competitive HMG CoA reductase Inhibitor. The amounts of cholesterol in Hep G2 decreased in total saponin-and ginsenoside-treated groups as compared with that of control group, while there was no significant reduction in lovastatin-treated group. The mRNA expression rates of HMG CoA reductase increased in total saponin and gin- senoside groups except for ginsenoside-Rb, (10-3%) group and decreased in lovastatin group com- pared with that of control group. The mRNA expression rates of LDL receptor generally increased In all of the test groups except for total saponin (10-5%) group compared with that of control group. Because the ginseng components tested were more effective in the reduction of cholesterol level in Hep G2 than lovastatin and induced the gene expression of LDL receptor, we suggest the possibility that they could be used as a replacement agent for lovastatin which can not be prescribed especially to patients with hepatic diseases.

• Advances in Traditional Medicine
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• 제9권2호
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• pp.106-114
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• 2009

Hepatocellular carcinoma is the world's most common primary malignant tumor of the liver. In-Jin-ho-Tang (IJHT) has been used as a traditional Chinese herbal medicine since ancient times, and today it is widely used as a medication for jaundice associated with inflammation of the liver. In-Jin-Ho-Tang is a drug preparation consisting of three herbs: Artemisiae Capillaris Herba (Artemisia capillaries $T_{HUNS}$, Injinho in Korean), Gardeniae Fructus (Gardenia jasminodes $E_{LLIS}$, Chija in Korean) and Rhei radix et rhizoma (Rheum palmatum L., Daehwang in Korean). This study investigated whether or not methanol extract of IJHT could induce HepG2 cancer cell death. Cytotoxic activity of IJHT on HepG2 cells was measured using an XTT assay, with an $IC_{50}$ value of $700{\mu}g/ml$ at 24 h Apoptosis induction by IJHT in HepG2 cells was verified by the cleavage of poly ADP-ribose polymerase, and a decrease in procaspase-3, -8, -9. Treatment of IJHT resulted in the release of cytochrome c into cytosol, loss of mitochondrial membrane potential (${\Delta}{\Psi}_m$), decrease in anti-apoptotic Bcl-2, and an increase in pro-apoptotic Bax expression. Thus, IJHT induced apoptosis in HepG2 cells via activation of caspase and mitochondria pathway. These results indicate that IJHT has potential as an anti-cancer agent.