• Journal of Plant Biotechnology
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• 제41권1호
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• pp.19-25
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• 2014

도라지는 초롱꽃과에 속하는 채소용 또는 약용으로 그 생산량이 해마다 증가하는 추세에 있다. 특히 국내의 경남지역에서는 토종도라지의 보존과 육성을 위한 정책을 추진하고 있고 도라지의 단가도 급등함에 따라 그 재배면적이 크게 증가하였다. 본 연구에서는 토종 도라지의 순계를 보존하기 위한 조직배양 기술의 확립과 함께 분자생물학적 기술을 이용한 토종 도라지의 판별 기술을 개발하기 위한 기초연구를 수행하였다. 먼저 경남지역과 충남아산시 등의 야산에서 채종하여 수 십년간 자가 채종하여 재배하여온 농가에서 수집한 종자를 중심으로 5종의 primer를 이용하여 RAPD분석을 수행한 결과 총 48개의 밴드를 생산하였으며, 이중 21개 밴드는 다형성을 보이는 밴드로 40.3%의 다형성을 나타내었다. 특히 primer HD3는 총 13개의 밴드 중 10개가 계통간 다형성을 보여서 76.9%의 높은 다형성빈도를 보였다. 향후 핵 DNA 보다는 핵외 DNA를 이용한 SNP 마커의 확보 등 보다 진보된 기술을 이용한 보완연구가 수행되어야 할 것으로 사료되었다.

• 한국환경보건학회지
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• 제48권6호
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• pp.324-330
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• 2022

Background: Lung injuries due to exposure to humidifier disinfectants (HDs) were reported in 2011 in South Korea. As a result of the government's epidemiological investigation and toxicity test study, it was found that HDs caused health damage such as lung disease. Objectives: The purpose of this study was to classify HD exposure ratings and analyze the affecting factors that could identify the relationship with lung disease. Methods: Exposure assessment for HDs was conducted using a questionnaire during face-to-face interviews with the applicants. Ratings of high exposure (Class 1) and low exposure (Class 2) were cross-tabulated with clinical ratings (acceptable and unacceptable). Logistic regression analysis was carried out by setting the clinical rating of lung disease as a dependent variable and the socio-demographic and exposure characteristics obtained through the questionnaire as independent variables. Results: The concentration in air of polyhexamethylene guanidine (PHMG) was 71.96±107.47 ㎍/m³, and the exposure concentration was 15.21±23.28 ㎍/m³ . The exposure rating was overestimated with 97.1% of affected subjects having high exposure using margin of exposure (MOE), but only 9.9% matching the clinical class. In the overestimated group, it could be explained by the fact that the exposure time was long and the subjects had already recovered from damage symptoms. As a result of logistic regression analysis, ten variables were found to be significant influencing factors. Conclusions: A new exposure rating could be calculated based on the MOE, and factors affecting lung disease could be estimated through comparative evaluation with the clinical rating.

• 방송공학회논문지
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• 제17권2호
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• pp.363-373
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• 2012

본 논문에서는 자유시점 또는 오토스테레오스코픽 비디오나 홀로그래픽 비디오 서비스 등을 목표로 중간시점 깊이영상을 생성하는 방법을 제안한다. 이 방법에서 좌/우 깊이영상이 주어졌다고 가정하고, 이 깊이영상들은 카메라 보정과 영상보정이 수행되었다고 가정한다. 제안한 방법은 깊이 당 변위증분을 계산하여 사용하며, 이 변위증분은 일반적인 상황을 고려하여 좌/우 깊이영상을 스테레오 정합하여 계산한다. 계산된 변위증분을 사용하여 해당 깊이값의 위치를 원하는 중간시점 깊이영상에서 찾는다. 중간시점 깊이영상은 좌영상과 우영상으로부터 각각 생성하며, 잡음을 제거하고 홀 필링을 수행한 후 두 영상을 결합하여 최종 중간시점 깊이영상을 생성한다. 제안한 방법을 구현하여 여러 테스트 시퀀스에 적용한 결과, 생성된 중간시점 깊이영상의 화질은 평균 PSNR 33.84dB이었으며, HD급 중간시점 깊이영상을 초당 한 프레임 정도 생성하는 속도를 보였다. 이 화질은 테스트 시퀀스들의 비균질성을 고려할 때 상당히 좋은 화질이라 판단되며, 향후 수행속도를 더욱 향상시키면 제안한 방법은 중간시점 깊이영상을 생성하는 좋은 방법이 될 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1085-1091
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• 2009

A phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. Therefore, this study used a degenerate PCR and TAIL PCR to clone a phytase gene from Erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. The full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kDa with a 50% amino acid identity to the Klebsiella pneumoniae phytase. The phytase contained the active site RHGXRXP and HD sequence motifs that are typical of histidine acid phosphatases. The enzyme was expressed in Escherichia coli, purified, and displayed the following characteristics: a high catalytic activity at low temperatures (retaining over 24% activity at $5^{\circ}C$) and remarkably thermal lability (losing >96% activity after incubation at $60^{\circ}C$ for 2 min). The optimal phytase activity occurred at pH 5.5 and ${\sim}49^{\circ}C$, and the enzyme activity rapidly decreased above $40^{\circ}C$. When compared with mesophilic counterparts, the phytase not only exhibited a high activity at a low temperature, but also had a low $K_m$ and high $k_{cat}$. These temperature characteristics and kinetic parameters are consistent with low-temperature-active enzymes. To our knowledge, this would appear to be the first report of a low-temperature-active phytase and its heterogeneous expression.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.971-979
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• 2012

Four, lactating dairy crossbreds ($50%{\times}50%$ Holstein Friesian${\times}$Native Zebu cattle) were randomly assigned according to a $2{\times}2$ factorial arrangement (two protein levels and two levels of mangosteen peel pellets (Mago-pel)) in a $4{\times}4$ Latin square design to receive four dietary treatments. All cows received concentrate at a proportion of 1 kg concentrate per 2 kg of milk yield, and urea-treated 5% rice straw (UTRS) was given ad libitum. It was found that total dry matter intakes, nutrient digestibility, ruminal pH and $NH_3$-N concentrations were not affected (p>0.05) by treatments. Concentrations of ruminal pH and $NH_3$-N were not affected by dietary treatments although the concentration of BUN varied significantly (p<0.05) between protein levels (p<0.05). The populations of rumen bacteria and fungal zoospores did not differ among treatments (p>0.05); however, the population of protozoa was decreased (p<0.05) when cows received Mago-pel supplementation. The composition of the population of bacteria, identified by real-time PCR technique, including total bacteria, methanogens, Fibrobacter succinogenes and Ruminococcus albus was similar (p>0.05) among dietary treatments (p>0.05); however, copy numbers of Ruminococcus flavefaciens was increased when protein level increased (p<0.05). Microbial protein synthesis, in terms of both quantity and efficiency, was enriched by Mago-pel supplementation. Milk yield was greatest in cows fed UTRS based diets with concentrate containing protein at 16% CP with Mago-pel, but were lowest without Mago-pel (p<0.05). In addition, protein level and supplementation of Mago-pel did not affect (p>0.05) milk composition except solids-not-fat which was higher in cows fed the diet with 19% CP. Therefore, feeding a concentrate containing 16% CP together with 300 g/hd/d Mago-pel supplementation results in changes in rumen fermentation and microbial population and improvements in milk production in lactating dairy crossbreds fed on UTRS.

• 천문학회보
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• 제35권1호
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• pp.57-57
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• 2010

We report the discovery of a bright mid-infrared (MIR) source with prominent crystalline silicate emission using the space telescope AKARI and Spitzer. This source, IRAS 15099-5856, has a spectacular morphology with a bright central compact source (CCS) surrounded by knots, spurs, and several extended (~4') arc-like filaments. The source is seen only in infrared at ${\geq}10{\mu}m$. The Spitzer MIR spectrum of the CCS shows prominent emission features from Mg-rich crystalline silicates and strong [Ne II] 12.88 ${\mu}m$ and several other faint ionic lines. We model the MIR spectrum as thermal emission from several independent dust components and compare their properties to those of the Herbig Be star HD 100546 which shows very similar MIR spectrum. Our molecular line observations reveal two molecular clouds around the source, but no associated dense molecular cores. We discuss two possible origins for IRAS 15099-5856; a deeply embedded massive young stellar object on the other side of the Galaxy and a disrupted, protoplanetary disk being photoevaporated by the UV radiation from the nearby O star Muzzio 10.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2010년도 춘계학술발표대회
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• pp.53.2-53.2
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• 2010

지금까지 능동 구동 디스플레이의 TFT backplane에 사용하고 있는 채널 물질로는 수소화된 비정질 실리콘(a-Si:H)과 저온 폴리실리콘(low temperature poly-Si)이 대표적이다. 수소화된 비정질 실리콘은 TFT-LCD 제조에 주로 사용되는 물질로 제조 공정이 비교적 간단하고 안정적이며, 생산 비용이 낮고, 소자 간 특성이 균일하여 대면적 디스플레이 제조에 유리하다. 그러나 a-Si:H TFT의 이동도(mobility)가 1 cm2/Vs이하로 낮아 Full HD 이상의 대화면, 고해상도, 고속 동작을 요구하는 UD(ultra definition)급 디스플레이를 개발하는데 있어 한계 상황에 다다르고 있다. 또한 광 누설 전류(photo leakage current)의 발생을 억제하기 위해서 화소의 개구율(aperture ratio)을 감소시켜야하므로 패널의 투과율이 저하되고, 게이트 전극에 지속적으로 바이어스를 인가 시 TFT의 문턱전압(threshold voltage)이 열화되는 문제점을 가지고 있다. 문제점을 극복하기 위한 대안으로 근래 투명 산화물 반도체(transparent oxide semiconductor)가 많은 관심을 얻고 있다. 투명 산화물 반도체는 3 eV 이상의 높은 밴드갭(band-gap)을 가지고 있어 광 흡수도가 낮아 투명하고, 광 누설 전류의 영향이 작아 화소 설계시 유리하다. 최근 다양한 조성의 산화물 반도체들이 TFT 채널 층으로의 적용을 목적으로 활발하게 연구되고 있으며 ZnO, SnO2, In2O3, IGO(indium-gallium oxide), a-ZTO(amorphous zinc-tin-oxide), a-IZO (amorphous indium-zinc oxide), a-IGZO(amorphous indium-galliumzinc oxide) 등이 그 예이다. 이들은 상온 또는 $200^{\circ}C$ 이하의 낮은 온도에서 PLD(pulsed laser deposition)나 스퍼터링(sputtering)과 같은 물리적 기상 증착법(physical vapor deposition)으로 손쉽게 증착이 가능하다. 특히 이중에서도 a-IGZO는 비정질임에도 불구하고 이동도가 $10\;cm2/V{\cdot}s$ 정도로 a-Si:H에 비해 월등히 높은 이동도를 나타낸다. 이와 같이 a-IGZO는 비정질이 가지는 균일한 특성과 양호한 이동도로 인하여 대화면, 고속, 고화질의 평판 디스플레이용 TFT 제작에 적합하고, 뿐만 아니라 공정 온도가 낮은 장점으로 인해 플렉시블 디스플레이(flexible display)의 backplane 소재로서도 연구되고 있다. 본 실험에서는 rf sputtering을 이용하여 증착한 a-IGZO 박막에 대하여 열처리 조건 변화에 따른 a-IGZO 박막들의 광학적, 전기적 특성변화를 살펴보았고, 이와 더불어 a-IGZO 박막을 TFT에 적용하여 소자의 특성을 분석함으로써, 열처리에 따른 Transfer Curve에서의 우리가 요구하는 Threshold Voltage(Vth)의 변화를 관찰하였다.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 1999년도 추계학술발표회 초록집
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• pp.15-15
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• 1999

In chip plating, several parameters must be taken into consideration. Current density, solution concentration, pH, solution temperature, components volume, chip and media ratio, barrel geometrical shape were most likely found to have an effect to the process yields. The 3 types of barrels utilized in chip plating industry are the conventional rotating barrel. vibrational barrel (vibarrel), and the centrifugal type. Conventional rotating barrel is a close type and is commonly used. The components inside the barrel are circulated by the barrel's rotation at a horizontal axis. Process yield has known to have higher thickness deviation. The vibrational barrel is an open type which offers a wide exposure to electrolyte resulting to a stable thickness deviation. It rotates in a vertical axis coupled with multi-vibration action to facilitate mixed up and easy transportation of components, The centrifugal barrel has its plated work centrifugally compacted against the cathode ring for superior electrical contact with simultaneous rotary motion. This experiment has determined the effect of barrel vibration intensity to the plating thickness distribution. The procedures carried out in the experiment involved the overall plating process., cleaning, rinse, Nickel plating, Tin-Lead plating. Plating time was adjusted to meet the required specification. All other parameters were maintained constant. Two trials were performed to confirm the consistency of the result. The thickness data of the experiment conducted showed that the average mean value obtained from higher vibrational intensity is nearer to the standard mean. The distribution curve shown has a narrower specification limits and it has a reduced variation around the target value, Generally, intensity control in vi-barrel facilitates mixed up and easy transportation of components, However, it is desirable to maintain an optimum vibration intensity to prevent solution intrusion into the chips' internal electrode. A cathodic reaction can occur in the interface of the external and internal electrode. $2HD{\;}+{\;}e{\;}{\rightarrow}20H{\;}+{\;}H_2$ Hydrogen can penetrate into the body and create pressure which can cause cracks. At high intensity, the chip's motion becomes stronger, its contact between each other is delayed and so plating action is being controlled. However, the strong impact created by its collision can damage the external electrode's structure thereby resulting to bad plating condition. 1 lot of chip was divided into two equal partion. Each portion was loaded to the same barrel one after the other. Nickel plating and tin-lead plating was performed in the same station. Portion A maintained the normal barrel vibration intensity and portion B vibration intensity was increased two steps higher. All other parameters, current, solution condition were maintained constant. Generally, plating method find procedures were carried out in a best way to maintained the best plating condition. After plating, samples were taken out from each portion. molded and polished. Plating thickness was investigated for both. To check consistency of results. 2nd trial was done now using different lot of another characteristics.

• 대한핵의학회지
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• 제37권3호
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• pp.171-177
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• 2003

목적: 악성 림프종 환자에서 골수 침범 여부를 평가하는 데 있어 FDG PET 스캔의 유용성을 장골 골수 생검에 비교하여 평가하고자 한다. 대상 및 방법: 73명의 림프종 환자(남:녀=43:30, 평균 연령 47세)를 대상으로 하였다. 이 중 69명은 비호지킨성 림프종이었으며 4명은 호지킨성 림프종이었다. FDG PET은53명의 환자에서 병기 결정을 목적으로, 20명에서는 치료후 효과 평가를 위해 시행되었다. 골수 침범에 대한 최종 판정은 두 검사의 일치성으로 판단되었으며 서로 다른 경우엔 장골 골수 생검, 방사선 검사 소견, 그리고 추적 검사에 의해 결정되었다. 결과: 73명중 54명(74%)에 있어 FDG PET과 장골 골수 검사 결과가 일치하였다. 두 검사 모두 음성인 환자는 54명중 47명이었으며 모두 양성인 환자는 7명이었다. 두 검사가 불일치 하는 경우는 19명 이었으며 이 중 6명은 골수 검사는 음성이었으나 FDG PET에서 양성 소견을 보였다. 반대로 12명에서는 FDG PET은 음성이었으나 장골 골수 생검상 골수 침범이 확인되었다. 불일치 결과를 보인 19명 중 마지막 1명의 환자에서는 골수 생검상 음성이었으나 FDG PET에서 위양성 소견을 보였다. 결론: FDG PET은 림프종 환자의 병기 결정에 있어 장골 골수 검사상 발견되지 않은 골수 침윤을 발견하는 데 도움이 되어 골수 생검에 보조적인 역할이 있었다. PET 검사상 횡격막 상하 림파절 침범과 다수의 골수 병변이 있는 환자에 있어 골수 생검의 필요성에 대해서는 치료 방침이나 예후를 변화시킬 수 있는지의 여부를 보는 연구가 필요하리라 생각된다. 치료후 효과를 판정하는 데 있어 FDG PET은 골수의 현미경학적 잔존 질환을 평가하는 데 있어 골수 생검을 대체하기는 어려울 것으로 사료된다.

• 한국자동차공학회논문집
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• 제24권5호
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• pp.538-548
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• 2016

The purpose of this study is to identify the possibility of effective tuning works, understand the characteristics of tuning engine, and analyse the basic data of engine tuning inspection corresponding to the safe operation and environment of a driving gasoline car. The effects of tuning on the characteristics of performance and exhaust emissions under a wide range of engine speeds are experimentally investigated by the actual driving car with a four-cycle, four-cylinder DOHC, turbo-intercooler, water-cooled gasoline engine operating at four types of non-tuning, tuning 1, 2 and 3. The tuning parts in the gasoline engine are the intake manifold, intake pipe and air filter. In the experiment, the output, torque and air-fuel ratio of the five-speed automatic transmission vehicles were measured at the chassis dynamometer(Dynojet 224xLC) with one person on board. The exhaust emissions of $NO_X$, THC, CO, $O_2$ and $CO_2$, and excess air ratio(${\lambda}$) at the other chassis dynamometer(DASAN-MD-ASM-97-KR-HD) were also measured by the idle/constant-speed mode(ASM2525 mode) test method. It is found that the actual air-fuel ratios of non-tuning and tuning engines were shown to be lower than the stoichiometric air-fuel ratio with increasing engine speed, and the actual air-fuel ratio of non-tuning engine was slightly higher than those of tuning engines when the engine speed is more than 4000 rpm. The output was significantly increased by the tuning whereby the maximum output of tuning engine was more increased to approximately 117.64% than that of non-tuning engine. In addition, CO, THC and $NO_X$ emissions of non-tuning and tuning engines measured by the constant-speed test mode were all satisfied with the inspection standards. CO emission was increased, while THC and $NO_X$ emissions were reduced by tuning.