• 한국식품과학회지
• /
• 제45권6호
• /
• pp.770-776
• /
• 2013

본 연구에서는 호박잎 추출물의 총 폴리페놀 함량, 총 플라보노이드 함량, radical 소거활성 등과 같은 항산화성과, 암세포 증식억제 활성, 사멸유도 활성, 부착억제 활성 등과 같은 항암성을 in vitro 수준에서 검증하였다. 호박잎의 총 폴리페놀 함량은 263.4 mg gallic acid equivalent/100 g, 총 플라보노이드 함량은 73.6 mg quercetin equivalent/100 g으로 측정되었다. 호박잎 추출물은 300 ${\mu}g/mL$ 농도에서 69.4%의 ABTS radical 소거 활성이 있었는데, 이는 호박잎 추출물과 같은 농도에서의 L-ascorbic acid 활성(94.5%)의 73.4%에 해당되는 수준이었다. 한편 호박잎 추출물은 37.5, 75, 150 ${\mu}g/mL$ 농도에서 HCT116 대장암 세포와 H1299 폐암 세포의 증식을 60.6-87.9% 억제하는 농도 의존적 활성이 있었으며 이는 부분적으로 apoptosis를 유도하기 때문인 것으로 생각된다. 또한 호박잎 추출물은 150 ${\mu}g/mL$ 농도에서 HCT116 세포와 H1299 세포의 부착을 16.8-28.4% 억제하는 활성이 있었다. 이상의 결과를 통하여 호박잎 추출물은 in vitro 수준에서 항산화성 및 항암성을 가지는 것으로 생각되며, 이러한 연구 결과는 향후 관련 작용기전을 규명하기 위한 기초 자료로 이용될 수 있을 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권6호
• /
• pp.3975-3978
• /
• 2013

Background: Proteases play a regulatory role in a variety of pathologies including cancer, pancreatitis, thromboembolic disorders, viral infections and many others. One of the possible strategies to combat these pathologies seems to be the use of protease inhibitors. LC-pi I, II, III and IV (Lavatera cashmerian-protease inhibitors) have been found in vitro to strongly inhibit trypsin, chymotrypsin and elastase, proteases contributing to tumour invasion and metastasis, indicated possible anticancer effects. The purpose of this study was to check in vitro anticancer activity of these four inhibitors on human lung cancer cell lines. Material and Methods: In order to assess whether these inhibitors induced in vitro cytoxicity, SRB assay was conducted with THP-1 (leukemia), NCIH322 (lung) and Colo205, HCT-116 (colon) lines. Results: LC-pi I significantly inhibited the cell proliferation of all cells tested and also LC-pi II was active in all except HCT-116. Inhibition of cell growth by LC-pi III and IV was negligible. $IC_{50}$ values of LC-pi I and II for NCIH322, were less compared to other cell lines suggesting that lung cancer cells are more inhibited. Conclusion: These investigations might point to future preventive as well as curative solutions using plant protease inhibitors for various cancers, especially in the lung, hence warranting their further investigation.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
• /
• pp.254.2-254
• /
• 1999

No Abstract, See Full Text

• 한국자원식물학회지
• /
• 제28권3호
• /
• pp.297-304
• /
• 2015

The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation.

• 한국식품조리과학회지
• /
• 제31권1호
• /
• pp.91-97
• /
• 2015

The present study was performed to investigate antioxidant and anti-colon cancer activities of red cabbage (Brassica oleracea L. var. capitata f. rubra DC) according to the cooking conditions (raw, microwave, blanching and steaming). The contents of red cabbage extracts were determined as follow: total phenolic contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethy lbenzo-thiazoline-6-sulfonic acid) (ABTS), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis. The total contents of polyphenol and flavonoid of red cabbage were 20.27 mg GAE/g Dry weight ${\pm}0.03$ and $2.55{\pm}0.02mg$ RE/g Dry weight. In this study, the total contents of polyphenol were decreased to both microwave and steam cooking. Total antioxidant activity and growth inhibition of HCT116 human colon cancer cells were in the order of raw > microwaving > steaming cooking methods. These results indicate that red cabbage extracts might have antioxidant and anti-proliferative activity according to the cooking conditions.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권8호
• /
• pp.3395-3402
• /
• 2015

Background: Preoperative 5-fluorouracil (5-FU)-based chemoradiotherapy is a standard treatment for locally advanced colorectal cancer (CRC). However, CRC cells often develop chemoradiation resistance (CRR). Recent studies have shown that long non-coding RNA (lncRNA) plays critical roles in a myriad of biological processes and human diseases, as well as chemotherapy resistance. Since the roles of lncRNAs in 5-FU-based CRR in human CRC cells remain unknown, they were investigated in this study. Materials and Methods: A 5-FU-based concurrent CRR cell model was established using human CRC cell line HCT116. Microarray expression profiling of lncRNAs and mRNAs was undertaken in parental HCT116 and 5-FU-based CRR cell lines. Results: In total, 2,662 differentially expressed lncRNAs and 2,398 mRNAs were identified in 5-FU-based CRR HCT116 cells when compared with those in parental HCT116. Moreover, 6 lncRNAs and 6 mRNAs found to be differentially expressed were validated by quantitative real time PCR (qRT-PCR). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for the differentially expressed mRNAs indicated involvement of many, such as Jak-STAT, PI3K-Akt and NF-kappa B signaling pathways. To better understand the molecular basis of 5-FU-based CRR in CRC cells, correlated expression networks were constructed based on 8 intergenic lncRNAs and their nearby coding genes. Conclusions: Changes in lncRNA expression are involved in 5-FU-based CRR in CRC cells. These findings may provide novel insight for the prognosis and prediction of response to therapy in CRC patients.

• BMB Reports
• /
• 제51권10호
• /
• pp.532-537
• /
• 2018

Prostaglandin $E_2$ ($PGE_2$), a major product of cyclooxygenase-2 (COX-2), plays an important role in the carcinogenesis of many solid tumors, including colorectal cancer. Because $PGE_2$ functions by signaling through $PGE_2$ receptors (EPs), which regulate tumor cell growth, invasion, and migration, there has been a growing amount of interest in the therapeutic potential of targeting EPs. In the present study, we investigated the role of EP4 on the effectiveness of cordycepin in inhibiting the migration and invasion of HCT116 human colorectal carcinoma cells. Our data indicate that cordycepin suppressed lipopolysaccharide (LPS)-enhanced cell migration and invasion through the inactivation of matrix metalloproteinase (MMP)-9 as well as the down-regulation of COX-2 expression and $PGE_2$ production. These events were shown to be associated with the inactivation of EP4 and activation of AMP-activated protein kinase (AMPK). Moreover, the EP4 antagonist AH23848 prevented LPS-induced MMP-9 expression and cell invasion in HCT116 cells. However, the AMPK inhibitor, compound C, as well as AMPK knockdown via siRNA, attenuated the cordycepin-induced inhibition of EP4 expression. Cordycepin treatment also reduced the activation of CREB. These findings indicate that cordycepin suppresses the migration and invasion of HCT116 cells through modulating EP4 expression and the AMPK-CREB signaling pathway. Therefore, cordycepin has the potential to serve as a potent anti-cancer agent in therapeutic strategies against colorectal cancer metastasis.

• Biomolecules & Therapeutics
• /
• 제31권1호
• /
• pp.73-81
• /
• 2023

Sirtuins (SIRTs) belong to the nicotinamide adenine dinucleotide (NAD+)-dependent class III histone deacetylase family. They are key regulators of cellular and physiological processes, such as cell survival, senescence, differentiation, DNA damage and stress response, cellular metabolism, and aging. SIRTs also influence carcinogenesis, making them potential targets for anticancer therapeutic strategies. In this study, we investigated the anticancer properties and underlying molecular mechanisms of a novel SIRT1 inhibitor, MHY2251, in human colorectal cancer (CRC) cells. MHY2251 reduced the viability of various human CRC cell lines, especially those with wild-type TP53. MHY2251 inhibited SIRT1 activity and SIRT1/2 protein expression, while promoting p53 acetylation, which is a target of SIRT1 in HCT116 cells. MHY2251 treatment triggered apoptosis in HCT116 cells. It increased the percentage of late apoptotic cells and the sub-G1 fraction (as detected by flow cytometric analysis) and induced DNA fragmentation. In addition, MHY2251 upregulated the expression of FasL and Fas, altered the ratio of Bax/Bcl-2, downregulated the levels of pro-caspase-8, -9, and -3 proteins, and induced subsequent poly(ADP-ribose) polymerase cleavage. The induction of apoptosis by MHY2251 was related to the activation of the caspase cascade, which was significantly attenuated by pre-treatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, MHY2251 stimulated the phosphorylation of c-Jun N-terminal kinase (JNK), and MHY2251-triggered apoptosis was blocked by pre-treatment with SP600125, a JNK inhibitor. This finding indicated the specific involvement of JNK in MHY2251-induced apoptosis. MHY2251 shows considerable potential as a therapeutic agent for targeting human CRC via the inhibition of SIRT1 and activation of JNK/p53 pathway.

• Food Science and Biotechnology
• /
• 제18권3호
• /
• pp.749-754
• /
• 2009

The polysaccharide (BB-pol) extracted from Bifidobacterium bifidum BGN4 showed growth inhibitory effects on several colon cancer cell lines such as HT-29 and HCT-116. To increase the yield of polysaccharide, B. bifidum BGN4 was cultured in various culture media with different compositions. When B. bifidum BGN4 was cultured in modified MRS broth containing phytic acid, the cells showed increased branch formation and enlarged morphology. The content of total carbohydrate and the ability of adhesion to intestinal epithelial cells were also increased by phytic acid. The polysaccharide obtained from the cells grown in the presence of phytic acid inhibited the proliferation of cancer cell lines such as HT-29 and MCF-7 cells but not normal colon cell line, FHC. Taken together, Bifidobacterium grown in the presence of phytic acid may confer enhanced beneficial function for the host.

• 생명과학회지
• /
• 제24권9호
• /
• pp.967-972
• /
• 2014

본 연구는 한국 고유의 전통주인 막걸리 제조시 생성되는 주박 추출물과 유기용매 분획물을 총 85종 분리하여, 이들의 대장암 세포주에 대한 항성장 활성과 활성기전을 연구하였다. 이들 중 세포생존율 연구결과에 따라 3가지 종류의 분획물(KSD-E1-3, KSD-E2-3, KSD-E4-3)을 선별하였다. 이 중 가장 활성이 높은 KSD-E1-3 분획물에 의한 유전체 수준에서의 유전자 발현변화를 확인하기 위하여 oligo DNA microarray 실험을 수행하였다. 그 결과, 2배이상 발현이 증가된 유전자들 가운데 항암 유전자인 NAG-1, ATF3, DDIT3 그리고 p21을 선별하여 추가 실험을 진행하였다. 세포생존율 결과에 따라 선별된 3가지 종류의 분획물(KSD-E1-3, KSD-E2-3 그리고 KSD-E4-3)에 의한 4가지 종류의 유전자의 발현을 확인한 결과, KSD-E1-3에 의하여 모든 유전자의 발현이 높게 증가되었다. KSD-E1-3에 의한 유전자의 발현이 전사조절인자 p53에 의존적인지 확인하고자 p53 null HCT116 세포주를 이용하여 RT-PCR한 결과, NAG-1, ATF3, 그리고 DDIT3 유전자 p53에 비의존적이었으며, 반면 p21 유전자는 p53에 의해서만 발현이 증가되는 것을 확인하였다. 이러한 연구 결과는 주박 추출물이 다양한 기작에 의해 항암 유전자의 발현을 유도함으로써 암 세포에 대한 항성장 활성을 보여주고 있음을 나타낸다.