• Title/Summary/Keyword: H1N1

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The Present and the Future of Biogas Purification and Upgrading Technologies (바이오가스 정제 및 고질화 기술 현황 및 전망)

  • Heo, Namhyo;Park, Jaekyu;Kim, Kidong;Oh, Youngsam;Cho, Byounghak
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.172-172
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    • 2011
  • Anaerobic digestion(AD) has successfully been used for many applications that have conclusively demonstrated its ability to recycle biogenic wastes. AD has been successfully applied in industrial waste water treatment, stabilsation of sewage sludge, landfill management and recycling of biowaste and agricultural wastes as manure, energy crops. During AD, i.e. organic materials are decomposed by anaerobic forming bacteria and fina1ly converted to excellent fertilizer and biogas which is primarily composed of methane(CH4) and carbon dioxide(CO2) with smaller amounts of hydrogen sulfide(H2S) and ammonia(NH3), trace gases such as hydrogen(H2), nitrogen(N2), carbon monoxide(CO), oxygen(O2) and contain dust particles and siloxanes. The production and utilisation of biogas has several environmental advantages such as i)a renewable energy source, ii)reduction the release of methane to the atomsphere, iii)use as a substitute for fossil fuels. In utilisation of biogas, most of biogas produced from small scale plant e.g. farm-scale AD plant are used to provide as energy source for cooking and lighting, in most of the industrialised countries for energy recovery, environmental and safety reasons are used in combined heat and power(CHP) engines or as a supplement to natural. In particular, biogas to use as vehicle fuel or for grid injection there different biogas treatment steps are necessary, it is important to have a high energy content in biogas with biogas purification and upgrading. The energy content of biogas is in direct proportion to the methane content and by removing trace gases and carbon dioxide in the purification and upgrading process the energy content of biogas in increased. The process of purification and upgrading biogas generates new possibilities for its use since it can then replace natural gas, which is used extensively in many countries, However, those technologies add to the costs of biogas production. It is important to have an optimized purification and upgrading process in terms of low energy consumption and high efficiency giving high methane content in the upgraded gas. A number of technologies for purification and upgrading of biogas have been developed to use as a vehicle fuel or grid injection during the passed twenty years, and several technologies exist today and they are continually being improved. The biomethane which is produced from the purification and the upgrading process of biogas has gained increased attention due to rising oil and natural gas prices and increasing targets for renewable fuel quotes in many countries. New plants are continually being built and the number of biomethane plants was around 100 in 2009.

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Effect of IRES Controlled Reporter Gene on Screening and Production of Recombinant Human EPO Proteins from Cultured CHO Cells

  • Lee Hyun Gi;Park Jin-Ki;Kim Sung-Woo;Ko Eun-Mi;Kim Byoung-Ju;Jo Su-Jin;Byun Sung-June;Yang Boh-Suk;Chang Won-Kyong;Lee Hoon-Taek;Lee Poong-Yeon
    • Reproductive and Developmental Biology
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    • v.30 no.2
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    • pp.81-85
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    • 2006
  • This study was conducted to examine the effect of IRES controlled reporter gene on screening and production of recombinant human erythropoietin (EPO) proteins from cultured CHO cells. The cDNA was cloned for EPO from human liver cDNA Using site-directed mutagenesis, we generated recombinant human EPO (rhEPO) with two additional N-glycosylations (Novel erythropoiesis-stimulating protein: NESP). Wild type hEPO and NESP were cloned into expression vectors with GFP reporter gene under regulatory control of CMV promoter and IRES so that the vectors could express both rhEPO and GFP. The expression vectors were transfected to cultured CHO-K1 cells. Under microscopy, expression of GFP was visible. Using supernatant of the culture, ELISA assay, immunocytochemistry and in vitro assay using EPO dependant cell line were performed to estimate biological activity to compare the production characteristics (secretion levels, etc.) between rhEPO and NESP. The activity of NESP protein, obtained by mutagenesis, was described and compared with its rhEPO counterpart produced under same conditions. Although NESP had less secretion level in CHO cell line, the biological activity of NESP was greater than that of rhEPO. These results are consistent with previous researches. We also demonstrated that rhEPO and GFP proteins expressed simultaneously from transfected CHO cell line. Therefore we conclude that use of GFP reporter gene under IRES control could be used to screen and produce rhEPO in cultured CHO cells.

In Sacco Evaluation of Rumen Protein Degradation Characteristics and In vitro Enzyme Digestibility of Dry Roasted Whole Lupin Seeds (Lupinus albus)

  • Yu, P.;Egan, A.R.;Leury, B.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.3
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    • pp.358-365
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    • 1999
  • The effects of dry roasting whole lupin seeds (lupinus albus, WLS) at 110, 130 or $150{^{\circ}C}$ for 15, 30 or 45 minutes on the in sacco rumen degradation characteristics, optimal heating conditions of time and temperature and in vitro enzyme digestibility were determined. Ruminant degradation characteristics (RDC) of crude protein (CP) of WLS were determined by in sacco technique in dairy cows. Measure ROC were soluble (S), undegradable (U), potentially degradable (D) fractions, lag time (TO) and rate of degradation (Kd) of insoluble but degradable fraction. Based on measured ROC, percentage bypass CP (%BCP) and bypass CP (BCP in g/kg, DM) were calculated. Degradability of CP was significantly reduced by dry roasting (p<0.001). The interaction of dry roasting temperature and time had significant effects on D (p<0.05), Kd (p<0.01), U (p<0.01), %BCP (p<0.001) and BCP (p<0.001) but not on S (p=0.923>0.05). With increasing time and temperature, S, D, Kd and U varied from 31.8%, 67.4%, 10.3%/h and 0.8% in the raw WLS (RWLS) to 27.1 %, 35.8%, 3.6%/h, 38.4% in $150{^{\circ}C}/45\;min$, respectively. All these effects resulted in increasing %BCP from 25.9 in RWLS to 61.0% in the $150{^{\circ}C}/45\;min$. Therefore BCP increased form 111.2 to 261.2 g/kg DM, respectively. Both %BCP and BCP at $150{^{\circ}C}/45\;min$ increased nearly 2.5 times over the RWLS. The effects of dry roasting on %BCP and BCP seemed to be linear up to the highest value tested. Although ROC had been altered by dry roasting, the In vitro perpsin-cellulase digestibility was generally unchanged. It was concluded that dry roasting was effective in shifting CP degradation from rumen to the lower gastrointestinal tract to potential reduce unnecessary N loss in the rumen. It might be of great value in successfully synchronizing the rhythms of release of nitrogen and energy in the rumen, thus achieving a more efficient fermentation of diets with high proportions of lignocellulosic resources. To determine the optimal dry roasting conditions, the digestibility of each treatment in the cows will be measured in the next trial using mobile bags technique.

Natural Environment of Silk Road (실크로드의 자연환경)

  • Son, Myoung-Won
    • Journal of the Korean association of regional geographers
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    • v.11 no.1
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    • pp.29-39
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    • 2005
  • Silk Road is the term involving all traffic roads connecting the East to the West, and ancient Korean culture has became globalized through Silk Road. In this paper, natural environment of Silk Road is considered as fundamental data in order to understand the Western culture introduced through Silk Road. Silk Road region is classified into Loess Plateau district; arid basin district; and high mountains district; Hesi Corridor(河西回廊) is the transition zone that the southeastern part of $W{\breve{u}}w{\bar{e}}i$(武威) is Loess Plateau district; other northwestern part is arid basin district Loess Plateau district, composed of homogeneous fines, has experienced dramatic soil loss by bringing under cultivation. At recent times, reforestation and construction of check-dam have been in operation to cope with this problems, Arid basin district; although at midlatitude, is desert climate because it is far from the ocean and is surrounded by high mountains. Oasis of Hesi Corridor is continuous linearly, but that of arid basin district is sporadic. In $H{\bar{a}}mi$ and $T{\breve{u}}l{\breve{u}}f{\bar{a}}n$(吐魯番) fruit-growing using karez flourishes, And glacier of $Ti{\bar{a}}nshian$(天山) Mts. and $Q{\bar{i}}lian$(祁連) Mts. is very important to arid basin district because of supplying water resource. In the tundra zone surrounding alpine glacier, are distributed widely solifluction lobes and earth hummocks.

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Emulsifying Properties of Surface-Active Substances from Defatted Rapeseed Cake by Supercritical Carbon Dioxide Extraction (초임계 $CO_2$ 유체 추출법으로 얻은 탈지 유채박 중 표면활성 물질의 유화 성질 연구)

  • Kim, Jeong-Won;Jeong, Yong-Seon;Lee, Eui-Seok;Gil, Na-Young;Kim, San-Seong;Kim, Hyun-Hyo;Kim, Tae Young;Lee, Yong-Hwa;Jang, Young-Seok;Lee, Ki-Teak;Hong, Soon-Taek
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.4
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    • pp.635-648
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    • 2013
  • This study was carried out to investigate the emulsifying properties of surface-active substances from defatted rapeseed cake by supercritical $CO_2$ extraction. Based on the interfacial tension data, a supercritical fluid extract (SFE) with the lowest value of 14.16 mN/m was chosen for evaluation which was obtained from No. 2 extraction condition (150 bar, $65^{\circ}C$, 250 g). For emulsions with SFE, some physicochemical properties (i.e., fat globule size, creaming stability, zeta potential etc) were investigated according to changes in SFE concentration, pH, and NaCl addition in an emulsion. It was found that fat globule size was decreased with increasing SFE concentration in emulsion, with showing a critical value at 0.5 wt%, thereby resulting in less susceptibility to creaming behavior. The SFE emulsion also showed instability at acidic conditions (pH<7.0) as well as by NaCl addition. This was coincided with zeta potential data of emulsion. In addition, SSL (sodium stearoyl lactylate) found to be suitable as a co-surfactant, as it helped considerably in decreasing fat globule size in emulsions and its optimum concentration to be over 0.03 wt%, based on 0.1 wt% SFE in emulsion.

Removal of Ammonia-Nitrogen Contained in Landfill Leachate by Ammonia Stripping(I) (암모니아 탈기공정을 이용한 침출수의 암모니아성 질소제거(I))

  • Lee, Byung-Jin;Cho, Soon-Haing
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.10
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    • pp.1893-1904
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    • 2000
  • Nitrogen compounds are one of the major pollutants which cause eutrophication problems of the river or lake and red tides problems of the ocean. Currently available technologies for the removal of nitrogen compounds are mostly biological treatment. However, biological treatment is only effective for the wastewater which contains low concentration of nitrogen compounds. Leachate from solid waste landfill or industrial wastewater which contains high concentration of nitrogen can not be effectively treated by most of the currently available biological treatment technologies. With this connection. the objective of this study is to examine the applicability of ammonia stripping technology for the removal of high concentration of ammonia nitrogen compounds of the leachate from solid waste landfill. It can be concluded that ammonia stripping technology which was placed before the biological treatment process was very effective for the removal of high concentration of ammonium compounds. The chemical cost for the ammonia stripping was 16 percent higher than MLE process, so other methods like sludge recycling are needed for the reduction of operation cost. Further details are discussed in this paper.

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포도 ‘켐벨얼리’의 예냉 및 저온 유통 기술 연구

  • 정대성;최선태;이미영
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.132-132
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    • 2003
  • 포도 중 캠벨얼리 품종은 색이 검붉고 당도가 높아 우리나라 대표적인 품종 중에 하나로 알려져있다. 그러나 수확 후 저장 유통 중 품질이 저하되기 쉽다. 따라서 예냉 조건 및 수송, 저장, 유통조건에 따른 품질조사를 비교실험하여 가장 안전한 저장 유통 방법을 개발하고자 하였다. 실험재료는 2003년 8월20일경에 포도 주산지인 영천 농가에서 수화 한 후 바로 예냉처리를 하였다. 이때 사용한 예냉기는 원예연구소에서 개발한 시제작품을 사용하였다. 대조구로 무예냉을 두었으며 예냉 후 수송온도도 관행적인 상온과 저온(2$^{\circ}C$)로 구분 처리하였으며 저온온도별로도 함께 실험하였다. 포장단위도 2kg 와 5kg로 각각 처리하여 수행하였다. 예냉이 품질에 미치는 영향을 조사하기 위하여 예냉처리에 따른 호흡패턴 변화와 이화학적 특성변화를 조사하였다. 또한 저장 중 중량감소율, 부패율 및 탈립율을 조사하였다. 포도 ‘켐벨얼리’의 수확 시 품온 29.5$^{\circ}C$를 적정 저장 온도인 2$^{\circ}C$로 저하시키는데 일반 저온 저장고에서 10시간 정도 걸리는 데 반하여 차압예냉기 이용시 3시간으로 단축시킬 수 있었으며 예냉 포도의 처리구별 호흡량은 초기 6.65$m\ell$/kg/hr 였으나 상온무예냉 및 예냉 처리구의 경우에는 저장해가면서 급격히 증가되었고 저온에서는 무예냉의 경우에는 계속 증가되는 반면 예냉 처리구의 경우에는 포장 규격과는 상관없이 다른 처리구 보다 호흡억제 효과가 있었다. Ethylene 발생량 역시 호흡과 비슷한 경향을 보여 주어 예냉 시 과실의 품질 저하 요인 발생량을 억제시킬 수 있었다. 경도는 저장 기간이 경과됨에 감소되나 저온 예냉 처리구의 경우가 초기 1.26N을 높게 유지하였고 자연중량 감모율은 상온저장 2주째 무예냉처리구 8.40%로 감소된데 비하여 예냉 저온 수송 구의 경우에는 0.25%로 매우 낮았다. 포도 ‘켐벨얼리’의 저온 저장중 부패율은 저장 2주째 상온 무예냉 처리구의 경우 19.8%이었으나 저온 예냉 처리구의 경우는 부패과가 발생하지 않았고 탈립율 역시 상온무예냉처리구의 경우 53.5%인데 반하여 저온 예냉 처리구는 저장 4주경과 시까지 탈림과가 없었다. 당도 및 pH변화 역시 차압 예냉 후 저온 수송 처리구가 초기당도(12.0Bx)$^{\circ}$값과 초기 pH(3.8)를 저장 25일 경과시까지 유지하였다. 수확 시 높은 품온을 갖고 있는 과일을 산지에서 예냉 처리를 한 후 저온 냉장차를 이용하여 유통한다면 관행 유통 구조보다 고품질의 포도를 유통시킬 수 있는 것으로 사료되며 앞으로는 완숙된 고 당도(12.0~15.0Bx)$^{\circ}$ 포도를 수확 한 즉시 예냉 처리하고 저온 유통한다면 보다 신선한 과일을 소비자에게 전달 할 수 있을 것이다.

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Characteristics of Hydrogen Storage in Ti-Cr-Mo and Ti-Cr-V bcc Alloys (Ti-Cr-Mo계 및 Ti-Cr-V계 bcc 합금의 수소저장특성에 관한 연구)

  • You, J.H.;Cho, S.W.;Park, C.N.;Choi, J.
    • Journal of Hydrogen and New Energy
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    • v.16 no.2
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    • pp.122-129
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    • 2005
  • The characteristics of hydrogen storage have been investigated in the Ti-Cr-Mo and Ti-Cr-V ternary alloys with bcc structure. The alloys were melted by arc furnace and remelted 4-5 times for homogeneity. The lattice parameters, microstructures and phases of the alloys were examined by SEM, EDX and XRD, and the Pressure-Composition isotherms of the alloys were measured. From these data the relationship of the maximum and effective hydrogen storage capacities vs. chemical composition, lattice parameter and the radius of tetrahedral site were analyzed and discussed. The results showed that all of these alloy, in the range of the this study, had mainly bcc solid solutions with small amount of Ti segregation due to a lower melting point of Ti compared with other elements. Lattice parameters of the alloys were very near to the atomic average values of lattice parameters of the constituent elements. It was also found that maximum hydrogen storage capacities of the Ti-Cr-Mo alloys increased with increasing Ti content and the radius of tetrahedral site but the effective hydrogen storage capacities decreased after showing the maximum. The hydrogen storage capacities of the Ti-Cr-V alloys were almost same even though the V contens were quite different from alloy to alloy and this could be attributed to the almost same Ti/Cr ratio of the alloys. The maximum effective hydrogen storage capacity of the Ti-Cr-Mo alloys was revealed at Ti content of about 40${\sim}$50 at% and radius of tetrahedral site of 0.43${\sim}$0.45 nm. The Ti-Cr-V alloys showed the hydrogen storage capacities of 3.0 wt% and effective hydrogen storage capacities of 1.5 wt%.

Biosynthesis of Isoprenoids: Characterization of a Functionally Active Recombinant 2-C-methyl-D-erythritol 4-phosphate Cytidyltransferase (IspD) from Mycobacterium tuberculosis H37Rv

  • Shi, Wenjun;Feng, Jianfang;Zhang, Min;Lai, Xuhui;Xu, Shengfeng;Zhang, Xuelian;Wang, Honghai
    • BMB Reports
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    • v.40 no.6
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    • pp.911-920
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    • 2007
  • Tuberculosis, caused by Mycobacterium tuberculosis, continues to be one of the leading infectious diseases to humans. It is urgent to discover novel drug targets for the development of antitubercular agents. The 2-C-methyl-Derythritol-4-phosphate (MEP) pathway for isoprenoid biosynthesis has been considered as an attractive target for the discovery of novel antibiotics for its essentiality in bacteria and absence in mammals. MEP cytidyltransferase (IspD), the third-step enzyme of the pathway, catalyzes MEP and CTP to form 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) and PPi. In the work, ispD gene from M. tuberculosis H37Rv (MtIspD) was cloned and expressed. With N-terminal fusion of a histidine-tagged sequence, MtIspD could be purified to homogeneity by one-step nickel affinity chromatography. MtIspD exists as a homodimer with an apparent molecular mass of 52 kDa. Enzyme property analysis revealed that MtIspD has high specificity for pyrimidine bases and narrow divalent cation requirements, with maximal activity found in the presence of CTP and $Mg^{2+}$. The turnover number of MtIspD is $3.4 s^{-1}$. The Km for MEP and CTP are 43 and $92{\mu}M$, respectively. Furthermore, MtIspD shows thermal instable above $50^{\circ}C$. Circular dichroism spectra revealed that the alteration of tertiary conformation is closely related with sharp loss of enzyme activity at higher temperature. This study is expected to help better understand the features of IspD and provide useful information for the development of novel antibiotics to treat M. tuberculosis.

자화수에서 염류의 용해속도 변화와 자화수에 의한 NaCl, KCl 및 석고의 결정화 양상에 대한 연구

  • Jeon, Sang Il;Kim, Dong Ryul;Lee, Suk Keun
    • Journal of the Korean Chemical Society
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    • v.45 no.2
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    • pp.116-120
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    • 2001
  • In order to know the physicochemical properties of magnetized water, the experimental methods of column assay, crystalization of saltsand gypsom have been explored to elucidate the effects of magnetized water on the solubility speed of salts, crystal pattern from salt squeous solutions, and gypsom crystal pattern, respectively. In the column assay for salt solubility the magnetized water showed the decreased initial solubility speed of NaCl and slightly increased initial solubility spped of KCI, however, the maximum solugilities of NaCl and KCI in the magnetized aster were almost same in the double distilled water, respectively. The column assay also indicated that the magnetized water showed the decreased initial solubility speed of urea (CH$_4$N$_2$O), sodium citrate (HOC(CO$_2$Na)-(CH$_2$CO$_2$Na)$_2$-2H$_2$O) and (NH$_4$)$_2$compared to the double distilled water, while slightly increased solubility speed of glycine (NH$_2$CH$_2$COOH), boric acid (H$_3$BO$_3$), MgSO$_4$. Crystalization of 1% or 5% salt aqueous solutions by rapid evaporation disclosed that the magnetized water produced more condensed and bigger crystal structure than the control water. The pattern of gypsom crystal formation also indicated that the magntized water enhanced the crystal formation in the hydration reaction of gypsom plaster compared to the double distilled water. Taken together, it was presumed that the magnetized water showed the different physicochemical properties in the interaction with various salts, especially showed the contrast results between NaCl and KCI.

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