• Title/Summary/Keyword: H143

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Effects of Soil Amendment Blended with Soldier Fly Casts and Coco Peat on Physicochemical Properties of Sand Soil (동애등에분변토와 코코피트가 혼합된 토양개량제가 모래의 이화학성에 미치는 영향)

  • Kim, Young-Sun;Lee, Sang-Beom;Ham, Suon-Kyu;Lim, Hye-Jung;Choi, Young-Cheol;Park, Kwan-Ho
    • Weed & Turfgrass Science
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    • v.3 no.2
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    • pp.143-149
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    • 2014
  • This study was conducted to evaluate the effects of the mixture ratio of soil amendments blended with coco peat (Coco) and American soldier fly cast (SFC) on the physicochemical properties on the sand green. pH and EC of soil were significantly related to mixture ratio of SFC, Mix1, Mix2 and Mix3. Capillary porosity, air-capillary porosity and total porosity of root zone mixed SFC, Coco, Mix1, Mix2 and Mix3 were met to the USGA green specification. The mixture ratios of Mix1 and Mix2 in root zone were positively related capillary porosity and total porosity, and air-capillary porosity was negatively related capillary porosity and hydraulic conductivity. Capillary porosity of Mix1, Mix2 and Mix3 blended SFC and Coco was affected by SFC and Coco, and total porosity by Coco. These results showed that soil amendments blended SFC and Coco was developed capillary porosity and hydraulic conductivity on the USGA sand green than these of SFC.

Survey of Yogurt Powder Storage in Ambient Export Countries A Safety Evaluation Standard Compliance and Comparative Analysis

  • Kim, Na-Kyeong;Park, Jung-Min;Lee, Jung-Hoon;Kim, Ha-Jung;Oh, Sejong;Imm, Jee-Young;Lim, Kwang-Sei;Kim, Jin-Man
    • Food Science of Animal Resources
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    • v.35 no.1
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    • pp.143-148
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    • 2015
  • Yogurt powder is fermented milk processed in the form of dry yogurt, and has advantages such as stability, storability, convenience, and portability. China and Vietnam are important export target countries because of the increased demand for dairy products. Therefore, we surveyed dairy product standardization in order to establish an export strategy. Lactic acid bacteria counts are unregulated in Korea and Vietnam. In China, lactic acid bacteria counts are regulated at $1{\times}10^6$ colonyforming units (CFU)/mL and detected at $6.24{\pm}0.33\;Log\;CFU/mL$. All three countries have regulated standards for total bacterial counts. In China, total bacterial counts of milk powder are regulated to n=5, c=2, m=50,000, M=200,000 and detected at $6.02{\pm}0.12\;Log\;CFU/mL$, exceeding the acceptable level. Lactic acid bacterial counts appeared to exceed total bacterial counts. Coliform group counts, Staphylococcus aureus, Listeria monocytogenes, and Salmonella species were not detected. Acidity is not regulated in Korea and Vietnam. In China, acidity was regulated to over $70^{\circ}T$ and detected $352.38{\pm}10.24^{\circ}T$. pH is unregulated in all three countries. pH was compared to that of general fermented milk, which is 4.2, and that of the sample was $4.28{\pm}0.01$. Aflatoxin levels are not regulated in Korea and China. In Vietnam, aflatoxin level is regulated at 0.05 ppb. Therefore, all ingredients of the yogurt powder met the safety standards. This data obtained in this study can be used as the basic data in assessing the export quality of yogurt powder.

Dosimetry of $^{222}Rn$ Component in Outdoor Natural Background Radiation (자연 방사선량 중 $^{222}Rn$기여 성분 정량 해석)

  • Jun, Jae-Shik;Oh, Hi-Peel;Chae, Ha-Suk;Lee, Byung-Young;Kim, Do-Sung
    • Journal of Radiation Protection and Research
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    • v.14 no.2
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    • pp.10-17
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    • 1989
  • A study for the assessment of dose given by outdoor radon to respiratory system has been carried out by making use of radon-cups containing CR-39 plastic track detectors. Detection efficiencies were determined by irradiation of the radon-cups in a standard radon chamber of known concentration. Thus determined detection factors of CR-39 plastic track detector in bare, open cup and filtered cup geometry are found to be 0.273, 0.0813 and 0.0371 $trmm^{-2}$/(37$Bqm^{-3}{\cdot}d$), respectively, which are chemically etched in 30% NaOH solution of $70^{\circ}C$ for 220 minutes. The outdoor radon concentrations measured at Taejeon(Chungnam National University) from May 1988 to March 1989 are in the range of 27.4 - 135.8 Bq/$m^3$(0.74 - 3.67pCi/l)by open cup and 16.7 - 143.9 Bq/$m^3$(0.45 - 3.89 pCi/l) by filtered cup, which yield overall annual average value of outdoor radon concentration of $70.8Bq/m^3$(1.91 pCi/l). Corresponding effective dose equivalent rate to respiratory system of ICRP standard man is assessed to be 520 nSv /h.

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Structure and Arterial Relaxing Activity of Substance P-related Peptides and Substance P analogs (Substance P-related peptide들과 유도체의 구조 및 혈관이완 활성)

  • KIM Eun Jung;KIM Chan-Hee;GO Hye-Jin;KIM In Hae;AN Sang Hyun;SOHN Hee-Young;PARK Hee Yeon;YOON Ho Dong;CHANG Young-Chae;HONG Yong-Ki;PARK Nam Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.3
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    • pp.143-147
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    • 2005
  • Relationship between structure and biological activity was performed using the five substance P-related peptides (SPRPs) [mammalian-SP (M-SP), cod-SP (C-SP), trout-SP (T-SP), lungfish-SP (L-SP) and Ranakinin] and four SP analogs [[$His^5$]-SP (5H-SP), [$Gly^5$]-SP (5G-SP), ($Ile^8$)-SP (8I-SP) and ($Trp^8$)-Ranakinin (8W-Ranakinin)]. The circular dichroism (CD) spectra showed that all of the peptides took an unordered structure in buffer solution and artificial liposomes. However, 8W-Ranakinin undergoes conformational changes by being transferred into neutral and acidic liposomes from an unordered structure to more ordered structure. The arterial relaxing effect of the peptides was also studied with guinea-pig aorta (GPA), As a result of the studies, L-SP was about 14-fold more potant than M-SP. The order of potency compared to $EC_{50}$ value was $L-SP{\gg}M-SP>5G-SP{\ge}8I-SP>5H-SP>T-SP$, C-SP, Ranakinin, 8W-Ranakinin.

The Effect of Job Environmental Factors on Job Satisfaction and Turnover Intention of Container Shipping Workers -Focused on the Difference between Land and Maritime Workers of 'H' Liner Shipping Company- (컨테이너 선사 종사자의 직무환경 요인이 직무만족도와 이직의도에 미치는 영향 - H사의 육상직과 해상직간 차이를 중심으로-)

  • Lee, Won-woo;Ryu, Hee-chan;Lee, Choong-bae
    • Journal of Korea Port Economic Association
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    • v.38 no.1
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    • pp.143-158
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    • 2022
  • The shipping industry has experienced significantly fluctuated while decline in long-term and boom in short-term period due to the continuous recession of the shipping industry since the 2010s and during the recent COVID-19 pandemic. This study aims to suggest implications for securing manpower and personnel policy by analyzing the effects of job environmental factors of a liner shipping company on job satisfaction and turnover intention and differences between land and marine workers. For the analysis, the job environmental factors of a liner shipping company were divided into work character, relational factors, personnel characters, and remuneration factors, and then structural equation model and t-test were conducted to test the relationship between job satisfaction and turnover intention. As a result of the study, it was found that job environmental factors, such as work, relationship, personnel, and remuneration, had a positive (+) effect on job satisfaction, and job satisfaction had a negative (-) effect on turnover intention. In a comparative analysis between groups such as land and sea workers, it was found that the maritime workers group had higher job satisfaction and lower turnover intention than the land workers.

Cultivation characteristics and yield of Sparassis latifolia depend on the substrate mixture (꽃송이버섯(Sparassis latifolia) 생육배지 조성에 따른 재배특성 및 수량)

  • Heo, Byong-Soo;Choi, Kyu-Hwan;Jo, Yeong-Min
    • Journal of Mushroom
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    • v.20 no.2
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    • pp.61-68
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    • 2022
  • We investigated the standard cultivation substrate for Sparassis latifolia "Neowul" bred in Jeollabuk-do Agricultural Research and Extension Services. Cultivation characteristics and yield were assessed by varying the kind of sawdust and additives, and the mixing ratio. The cultivation period in larch sawdust was the shortest at 97 days. The yield was excellent (143.6 g). The findings indicated that larch is a tree species appropriate for the cultivation of S. latifolia. When the additives were varied, the yield and productivity (53.1%) were highest (116.6 g) for the wheat bran treatment. Thus, wheat bran would be an additive appropriate for culturing S. latifolia. Concerning the use of different mixing ratios, larch sawdust:beet-pulp:wheat bran ratios of 80:15:5 and 85:10:5 resulted in yields of 114.4 g and 111.4 g, and productivity of 52.5% and 51.8%, respectively. These yield and productivity values were not statistically different. Thus, the standard cultivation substrate for S. latifolia can comprise larch sawdust, beet pulp, and wheat bran at a ratio of 80:15:5 or 85:10:5. The carbon/nitrogen (C/N) ratio assumed to be appropriate for the cultivation of S. latifolia was 184-223. Pinheading would be difficult below a C/N substrate ratio of 105. Thus, the C/N ratio of the media, as well as the pH, would be vital factors affecting pinheading during S. latifolia cultivation.

Surface Plasmon Resonance Imaging Analysis of Hexahistidine-tagged Protein on the Gold Thin Film Coated with a Calix Crown Derivative

  • Chung, Bong-Hyun;Baek, Seung-Hak;Shin, Yong-Beom;Kim, Min-Gon;Ro, Hyeon-Su;Kim, Eun-Ki
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.2
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    • pp.143-146
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    • 2004
  • A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

A Study on Soil Pollution Quality of Chungbuk Province by Soil Network (토양측정망에 의한 충북지역 토양오염도)

  • 김재용;박선희
    • Journal of environmental and Sanitary engineering
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    • v.16 no.4
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    • pp.77-89
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    • 2001
  • The research is intended to evaluate the soil pollution quality of Chungbuk province with Soil Network which is executed every year. The survey executed with Soil Network in 1999 represented that the soil quality of Chungbuk province was relatively good as the average concentrations of contaminants in soil were Cd: 0.146 mg/17g, Cu: 3.143 mgHg, As: 0.636 mg/kg, Hg: 0.012 mg/kg, Pb): 4.505 mg/kg, $Cr^{+6}$ not detected, and the average pH was 6.1. The reckoned soil Pollution score (SPS) of Chungbuk province has been shown much low SPS distribution in the range from 1.6 to 141.7, average 31.0. However, it has been represented relatively high SPS distribution at Chungju, Tanyang, and Chinchon, respectively, It also huts represented high SPS distribution not only at agricultural area and waste area for the Pollution sources but at a paddy field and dry field for land use. The classification of soil pollution class(SPC) based on soil pollution score(SPS) was SPC 1 under 100 of SPS at 212 areas which were 98.6% of total 215 areas and SPC 2 over 100 of SPS at only three area left. There was no area exceeding soil pollution value among the whole Soil Network areas investigated, and the whole average for each items was much lower level th퍼n soil Pollution value was. When the average concentration of heavy metals and the average pH in soil of Chungbuk province are compared with the natural contents of that in Korea, the research is concluded that the average concentration of heavy met is relatively similar to the natural contents of that and the average pH in soil of Chungbuk province is a little higher than the natural contents of that. From this method, soil quality of most of the Soil Network area was estimated to be healthy.

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A Novel Oxidative Stress-inducible Peroxidase Promoter and Its Applications to Production of Pharmaceutical Proteins in Transgenic Cell Cultures

  • Lee, Ok-Sun;Park, Sun-Mi;Kwon, Suk-Yoon;Lee, Haeng-Soon;Kim, Kee-Yeun;Kim, Jae-Whune;Kwak, Sang-Soo
    • Journal of Plant Biotechnology
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    • v.4 no.4
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    • pp.143-150
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    • 2002
  • A strong oxidative stress-inducible peroxidase promoter (referred to as SWPA2 promoter) was cloned from tell cultures of sweetpotato (Ipomoea batatas) and characterized in transgenic tobacco cultured cells in terms of biotechnological applications. Employing a transient expression assay in tobacco protoplasts, with five different 5'-deletion mutants of the SWPA2 promoter fused to the $\beta$-glucuronidase (GUS) reporter gene, the 1314 bp deletion mutant showed approximately 30 times higher GUS expression than the CaMV 35S promoter. The expression of GUS activity in suspension cultures of transgenic cells derived from transgenic tobacco leaves containing the -1314 bp SWPA2 promoter-GUS fusion was strongly expressed following 15 days of subculture compared to other deletion mutants, suggesting that the 1314 bp SWPA2 promoter will be biotechnologically useful for the development of transgenic cell lines engineered to produce key pharmaceutical proteins. In this respect, we developed transgenic cell lines such as tobacco (Nicotiana tabacum L. BY-2), ginseng (Panax ginseng) and Siberian ginseng (Acanthopanax senticosus) using a SWPA2 promoter to produce a human lactoferrin (hLf) and characterized the hLf production in cultured cells. The hLf production monitored by ELISA analysis in transgenic BY-2 cells was directly increased proportional to cell growth and reached a maximal level (up to 4.3% of total soluble protein) at the stationary phase in suspension cultures. The SWPA2 promoter should result in higher productivity and increased applications of plant cultured cells for the production of high-value recombinant proteins.

Making of Dongchimi Naengmyeun Broth Which Has Enhanced Antioxidant Activity Using Purple Sweet Potato (자색고구마를 사용한 항산화 활성이 강화된 동치미 냉면육수 제조)

  • Seo, Weon-Taek;Kim, Han-Gon;Lee, Jin-Sung;Cho, Kye-Man
    • Korean Journal of Microbiology
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    • v.47 no.2
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    • pp.143-150
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    • 2011
  • Lactic fermented dongchimi naengmyeun broth which has enhanced antioxidative activity was developed. During lactic fermentation of dongchimi naengmyeun broth at $25^{\circ}C$, changes of total lactic acid bacteria, pH, acidity, soluble phenolics, and anthocyanins were investigated. After 72 h of fermentation, the stronger antioxidant activities were observed in dongchimi naengmyeun broth supplemented with purple sweet potato than those of control dongchimi naengmyeun broth which showing 96.80% in DPPH radical scavenging activity, 100.82% in $ABTs^{+{\cdot}}$ scavenging activity, 7.77 in reducing power, and 6.89 in ferric reducing/antioxidant power, respectively. These high antioxidant activities related with higher contents of soluble phenolics and anthocyanins in dongchimi naengmyeun broth supplemented with purple sweet potato. The results suggest that the making of functional dongchimi naengmyeun broth by using high soluble phenolics and anthocyanins supplements such as purple sweet potato powder was possible.