• Title/Summary/Keyword: H1-H2 measure

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Evaluation of the thermal environments and the workload of farmers during the spraying pesticide in the rice field (농약 방제 작업자의 작업 환경 및 노동 부담 평가)

  • 최정화;이주영
    • Journal of the Korean Society of Clothing and Textiles
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    • v.26 no.11
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    • pp.1672-1681
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    • 2002
  • To evaluate the thermal environments and the workload of farmers in the rice field in summer, this study investigated rice farmers' physiological, psychological responses, work postures, work clothes, air temperature and air humidity during the spraying pesticide in the rice field. Five career farmers (3 males, 2 females) volunteered as the subjects. During the spraying pesticide in the rice field, physiological responses were monitored continuously. The results were as follows. l. Farmers wore only raincoats not pesticide-proof clothing. 2. The value of WBGT, rectal temperature($T_{re}$), mean skin temperature(${\={T}}_{sk}$) were $24.9∼28.9^{\circ}C,\;37.8({\pm}0.3)^{\circ}C\;and\;33.6({\pm}0.6)^{\circ}C$, respectively. Clothing microclimate temperature($T_{cl}$) on the chest and back were $32.5({\pm}2.6)^{\circ}C\;and\;33.6({\pm}2.6)^{\circ}C$, respectively(p<0.00l). Humidity inside of the clothing ($H_{cl}$) was over 80%RH and heart rate(HR) was 112(${\pm}27$)bpm. We evaluated that the spraying pesticide was 'heavy work' by the Tre and HR. To four subjective questionnaires, all farmers expressed 'hard, hot, humid and uncomfortable' without individual difference at the end of works. We suggested that 1) the spraying pesticide in the rice field was a heavy work, 2) because the workload of farmers in the raincoat/pesticide-proof clothing can't be evaluated by only WBGT, assessors should measure physiological, psychological responses as well as thermal environments, 3) to alleviate farmers' heat strain, clothing manufacturers must consider not only the improvement of textile materials and clothing weight but also the designing of personal cooling equipment.

Effects of Reactive Oxygen Species and Nitrogen Species on the Excitability of Spinal Substantia Gelatinosa Neurons

  • Park, Joo Young;Park, Areum;Chun, Sang Woo
    • International Journal of Oral Biology
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    • v.41 no.3
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    • pp.141-147
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    • 2016
  • Reactive oxygen species (ROS) and nitrogen species (RNS) are both important signaling molecules involved in pain transmission in the dorsal horn of the spinal cord. Xanthine oxidase (XO) is a well-known enzyme for the generation of superoxide anions ($O_2^{\bullet-}$), while S-nitroso-N-acetyl-DL-penicillamine (SNAP) is a representative nitric oxide (NO) donor. In this study, we used patch clamp recording in spinal slices of rats to investigate the effects of $O_2^{\bullet-}$ and NO on the excitability of substantia gelatinosa (SG) neurons. We also used confocal scanning laser microscopy to measure XO- and SNAP-induced ROS and RNS production in live slices. We observed that the ROS level increased during the perfusion of xanthine and xanthine oxidase (X/XO) compound and SNAP after the loading of 2',7'-dichlorofluorescin diacetate ($H_2DCF-DA$), which is an indicator of intracellular ROS and RNS. Application of ROS donors such as X/XO, ${\beta}-nicotinamide$ adenine dinucleotide phosphate (NADPH), and 3-morpholinosydnomimine (SIN-1) induced a membrane depolarization and inward currents. SNAP, an RNS donor, also induced membrane depolarization and inward currents. X/XO-induced inward currents were significantly decreased by pretreatment with phenyl N-tert-butylnitrone (PBN; nonspecific ROS and RNS scavenger) and manganese(III) tetrakis(4-benzoic acid) porphyrin (MnTBAP; superoxide dismutase mimetics). Nitro-L-arginine methyl ester (NAME; NO scavenger) also slightly decreased X/XO-induced inward currents, suggesting that X/XO-induced responses can be involved in the generation of peroxynitrite ($ONOO^-$). Our data suggest that elevated ROS, especially $O_2^{\bullet-}$, NO and $ONOO^-$, in the spinal cord can increase the excitability of the SG neurons related to pain transmission.

Development of control system for complex microbial incubator (복합 미생물 배양기의 제어시스템 개발)

  • Hong-Jik Kim;Won-Bog Lee;Seung-Ho Lee
    • Journal of IKEEE
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    • v.27 no.1
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    • pp.122-126
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    • 2023
  • In this paper, a control system for a complex microbial incubator was proposed. The proposed control system consists of a control unit, a communication unit, a power supply unit, and a control system of the complex microbial incubator. The controller of the complex microbial incubator is designed and manufactured to convert analog signals and digital signals, and control signals of sensors such as displays using LCD panels, water level sensors, temperature sensors, and pH concentration sensors. The water level sensor used is designed and manufactured to enable accurate water level measurement by using the IR laser method with excellent linearity in order to solve the problem that existing water level sensors are difficult to measure due to foreign substances such as bubbles. The temperature sensor is designed and used so that it has high accuracy and no cumulative resistance error by measuring using the thermal resistance principle. The communication unit consists of two LAN ports and one RS-232 port, and is designed and manufactured to transmit signals such as LCD panel, PCT panel, and load cell controller used in the complex microbial incubator to the control unit. The power supply unit is designed and manufactured to supply power by configuring it with three voltage supply terminals such as 24V, 12V and 5V so that the control unit and communication unit can operate smoothly. The control system of the complex microbial incubator uses PLC to control sensor values such as pH concentration sensor, temperature sensor, and water level sensor, and the operation of circulation pump, circulation valve, rotary pump, and inverter load cell used for cultivation. In order to evaluate the performance of the control system of the proposed complex microbial incubator, the result of the experiment conducted by the accredited certification body showed that the range of water level measurement sensitivity was -0.41mm~1.59mm, and the range of change in water temperature was ±0.41℃, which is currently commercially available. It was confirmed that the product operates with better performance than the performance of the products. Therefore, the effectiveness of the control system of the complex microbial incubator proposed in this paper was demonstrated.

Clinical assessment of whitening efficacy and safety of in-office tooth whitening system containing 15% hydrogen peroxide with or without light activation (15% 과산화수소 함유 전문가용 치아 미백제의 광활성화 여부에 따른 미백효과 및 안전성에 관한 임상평가)

  • Noh, Young-Suk;Rho, Young-Jee;Yoo, Yeon-Jee;Lee, Hyang-Ok;Lim, Sang-Min;Kweon, Hyun-Jeong;Kim, Ye-Un;Park, Seong-Yeon;Yoon, Hee-Young;Lee, Jung-Hyun;Lee, Chan-Hee;Oh, So-Ram;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
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    • v.36 no.4
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    • pp.306-312
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    • 2011
  • Objectives: This clinical study evaluated the effect of light activation on the whitening efficacy and safety of in-office bleaching system containing 15% hydrogen peroxide gel. Materials and Methods: Thirty-three volunteers were randomly treated with (n = 17, experimental group) or without light activation (n = 16, control group), using Zoom2 white gel (15% $H_2O_2$, Discus Dental) for a total treatment time of 45 min. Visual and instrumental color measurements were obtained using Vitapan Classical shade guide and Shadepilot (DeguDent) at screening test, after bleaching, and 1 month and 3 month after bleaching. Data were analyzed using t-test, repeated measure ANOVA, and chi-squared test. Results: Zoom2 white gel produced significant shade changes in both experimental and control group when pre-treatment shade was compared with that after bleaching. However, shade difference between two groups was not statistically significant (p > 0.05). Tooth shade relapse was not detected at 3 months after bleaching. The incidence of transient tooth sensitivity was 39.4%, with being no differences between two groups. Conclusions: The application of light activation with Zoom2 white gel system neither achieved additional whitening effects nor showed more detrimental influences.

The Effect of Ginkgo Biloba Extract on the Fractionsted Radiation Therapy in C3H Mouse Fibrosarcoma (Ginkgo Biloba Extract가 C3H 마우스 섬유육종의 분할 방사선치료에 미치는 영향)

  • Kim, Jong-Hoon;Ha, Sung-Whan;Park, Charn-Il
    • Radiation Oncology Journal
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    • v.20 no.2
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    • pp.155-164
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    • 2002
  • Purpose : A ginkgo biloba extract (GBE) has been known as a hypoxic cell radiosensitizer. Its mechanisms of action are increase of the red blood cell deformability, decrease the blood viscosity, and decrease the hypoxic cell fraction in the tumor. The aims of this study were to estimate the effect of GBE on fractionated radiotherapy and to clarify the mechanism of action of the GBE by estimating the blood flow in tumor and normal muscle. Materials and Methods : Fibrosarcoma (FSall) growing in a C3H mouse leg muscle was used as the tumor model. When the tumor size reached 7 mm in diameter, the GBE was given intraperitoneally at 1 and 25 hours prior to irradiation. The tumor growth delay was measured according to the various doses of radiation (3, 6, 9, 12 Gy and 15 Gy) and to the fractionation (single and fractionated irradiation) with and without the GBE injection. The radiation dose to the tumor the response relationships and the enhancement ratio of the GBE were measured. In addition, the blood flow of a normal muscle and a tumor was compared by laser Doppler flowmetry according to the GBE treatment. Results : When the GBE was used with single fraction irradiation with doses ranging from 3 to 12 Gy, GBE increased the tumor growth delay significantly (p<0.05) and the enhancement ratio of the GBE was 1.16. In fractionated irradiation with 3 Gy per day, the relationships between the radiation dose (D) and the tumor growth delay (TGD) were TGD $(days)=0.26{\times}D$ (Gy)+0.13 in the radiation alone group, and the TGD $(days)=0.30{\times}D$ (Gy)+0.13 in the radiation with GBE group. As a result, the enhancement ratio was 1.19 ($95\%$ confidence interval; $1.13\~1.27$). Laser Doppler flowmetry was used to measure the blood flow. The mean blood flow was higher in the muscle (7.78 mL/100 g/min in tumor and the 10.15 mL/100 g/min in muscle, p=0.005) and the low blood flow fraction (less than 2 mL/100 g/min) was higher in the tumor $(0.5\%\;vs.\;5.2\%,\;p=0.005)$. The blood flow was not changed with the GBE in normal muscle, but was increased by $23.5\%$ ( p=0.0004) in the tumor. Conclusion : Based on these results, it can be concluded that the GBE enhanced the radiation effect significantly when used with fractionated radiotherapy as well as with single fraction irradiation. Furthermore, the GBE increased the blood flow of the tumor selectively.

Effect of Forced Oral Administration of Excessive Tap water on the Water Content and Histological Changes of Some Muscles of Korean Cattle (강제급수(强制給水)가 우근육(牛筋肉)의 함수율(含水率)과 조직학적성상(組織學的性狀)에 미치는 영향(影響))

  • Choi, H.I.;Hong, B.W.
    • Korean Journal of Veterinary Research
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    • v.12 no.1
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    • pp.127-132
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    • 1972
  • Experimental studies on the water content of muscles and the histological changes of muscles, digestive tract and kidney, influenced by forced oral administration of excessive tap water, were performed by using 10 Korean bulls. Experimental animals were divided into two groups, five heads for test group and five for control group. All of the bulls used in this experiment were weighing between 250 kg and 300 kg and 3 and 4 years of age. In each test animal, a total of 140 litters of tap water was passed in the forestomach by means of catheterization within 12 to 18 hours. And each of them was slaughtered immediately after the animals showing symptoms of respiratory distress. In control group, the animals were allowed to drink tap water normally. From test and control animals after slaughter, each 10 gm of M. biceps femoris, M. satorius, M. adductor, M. gluteous supercialis, M. iliocostalis lumborum, and M. transversus costarum were taken from definite parts in order to measure water content. In the histopathological studies tissues of rumen, reticulum, omasum, abomasum, duodenum, colon and kidney were taken as wall as the above mentioned muscles. The results obtained were as follows; 1. Increase of water content in the muscles of test group were 4.6% in M. satorius, 4.24% in M. transversus costarum, 4.14% in M. gluteous supercialis, 4.02% in M. adductor, 3.88% in M. biceps femoris, and 2.46% in M. iliocostalis lumborum respectively. The highest increase was found in M. satorius and the lowest in M. iliocostalis lumborum. 2. In test animal, average increasing value of water content in muscles was 3.9% and shown highly significant (p<0.01). 3. On the microscopical findings of carcass, marked watery edema was observed all of the subcutaneous tissues and intermuscular connective tissues. 4. Microscopically, the epimysium, perimysium and endomysium were widened, and the muscle fibers were lacerated. The cells of stratum lucidum in the epithelium of rumen and reticulum were shown marked vacuolization. In the kidney, dilatation of Bowman's spaces and proximal tubles was observed.

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Evaluation of sensitivity of soil respiration to temperature in different forest types and developmental stages of maturity using the incubation method

  • Lee, Eun-Hye;Suh, Sang-Uk;Lee, Chang-Seok;Lee, Jae-Seok
    • Journal of Ecology and Environment
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    • v.35 no.1
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    • pp.1-7
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    • 2012
  • To calculate and predict soil carbon budget and cycle, it is important to understand the complex interrelationships involved in soil respiration rate (Rs). We attempted to reveal relationships between Rs and key environmental factors, such as soil temperature, using a laboratory incubation method. Soil samples were collected from mature deciduous (MD), mature coniferous (MC), immature deciduous (ID), and immature coniferous (IC) forests. Prior to measure, soils were pre-incubated for 3 days at $25^{\circ}C$ and 60% of maximum water holding capacity (WHC). Samples of gasses were collected with 0, 2, and 4 h interval after the beginning of the measurement at soil temperatures of 5, 15, 25, and $35^{\circ}C$ (at 60% WHC). Air samples were collected using a syringe attached to the cap of closed bottles that contained the soil samples. The $CO_2$ concentration of each gas sample was measured by gas chromatography. Rs was strongly correlated with soil temperature (r, 0.93 to 0.96; P < 0.001). For MD, MC, ID, and IC soils taken from 0-5 cm below the surface, exponential functions explained 90%, 82%, 92%, and 86% of the respective data plots. The temperature and Rs data for soil taken from 5-10 cm beneath the surface at MD, MC, ID, and IC sites also closely fit exponential functions, with 83%, 95%, 87%, and 89% of the data points, respectively, fitting an exponential curve. The soil organic content in mature forests was significantly higher than in soils from immature forests (P < 0.001 at 0-5 cm and P < 0.005 at 5-10 cm) and surface layer (P = 0.04 at 0-5 cm and P = 0.12). High soil organic matter content is clearly associated with high Rs, especially in the surface layer. We determined that the incubation method used in this study have the possibility for comprehending complex characteristic of Rs.

Effects of injection of hydrolysis plasma protein solution on the antioxidant properties in porcine M. Longissimus Lumborum

  • Seo, Hyun-Woo;Seo, Jin-Kyu;Yang, Han-Sul
    • Journal of Animal Science and Technology
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    • v.58 no.8
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    • pp.31.1-31.8
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    • 2016
  • Background: Plasma protein hydrolysates have been shown to possess antioxidant activity. However, no report has yet to examine the antioxidant effects of injection of plasma protein hydrolysates on meat quality. Therefore, in this study, the effects of injection of hydrolysis plasma protein solution on meat quality and storability were investigated in porcine M. longissimus lumborum. Methods: Twelve pigs were randomly selected at a commercial slaughter plant and harvested. Dissected loins were injected with one of five solutions: C- control (untreated), T1- 10 mM phosphate buffer solution (PBS), T2- 10 mM PBS with 0.01 % butylated hydroxytoluene, T3- 10 mM PBS with 5 % plasma proteins, and T4- 10 mM PBS with 5 % hydrolysis plasma proteins. Results: T3 and T4 induced greater reduction in protein content of the loin muscle than other treatments. T2 resulted in the lowest pH as well as highest cooking loss. After a storage period of 3-7 days, both lightness and redness of meat were unaffected by all injection treatments. However, yellowness was significantly elevated by treatment with T4 relative to the control. T4 also resulted in the lowest shear force (a measure of meat toughness), suggesting improvement of texture or tenderness. Further, T4 resulted in the most stable TBARS values during storage, indicating that this treatment might retard rancidity in meat. Conclusion: Injection of porcine M. longissimus lumborum with hydrolysis plasma protein solution could improve overall pork quality, including tenderness and storability.

Experimental Study of Flow Fields around a Perforated Breakwater

  • Ariyarathne, H.A. Kusalika S.;Chang, Kuang-An;Lee, Jong-In;Ryu, Yong-Uk
    • International Journal of Ocean System Engineering
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    • v.2 no.1
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    • pp.50-56
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    • 2012
  • This study investigates flow fields and energy dissipation due to regular wave interaction with a perforated vertical breakwater, through velocity data measurement in a two-dimensional wave tank. As the waves propagate through the perforated breakwater, the incoming wave energy is reflected back to the ocean, dissipated due to very turbulent flows near the perforations and inside the chamber, and transmitted through the perforations of the breakwater. This transmitted energy is further reduced due to the presence of the perforated back wall. Hence most of the energy is either reflected or dissipated in the vicinity of the structure, and only a small amount of the incoming wave energy is transmitted through the structure. In this study, particle image velocimetry (PIV) technique was employed to measure two-dimensional instantaneous velocity fields in the vicinity of the structure. Measured velocity data was treated statistically, and used to calculate mean flow fields, turbulence intensity and turbulent kinetic energy. For investigation of the flow pattern, time-averaged mean velocity fields were examined, and discussed using the cross-sections through slot and wall for comparison. Flow fields were obtained and compared for various cases with different regular wave conditions. In addition, turbulent kinetic energy was estimated as an approach to understand energy dissipation near the perforated breakwater. The turbulent kinetic energy was distributed against wave height and wave period to see the dependence on wave conditions.

Lysimetric Analysis for Transpiration and Carbon Accumulation of Cnidium officinale Makino in Hot Weather Conditions (고온조건에서 라이시미터를 이용한 천궁의 증산 및 탄소축적량 분석)

  • Seo, Young Jin;Kim, Kwang Seop;Kim, Dong Chun;Nam, Hyo Hoon;Kim, Jun Hyung;Lee, Bu Yong
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.6
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    • pp.463-470
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    • 2020
  • Background: Evaluation of transpiration is required for agricultural and environmental management applications, as crop yields and plant growth are primarily water limited. This study aimed to determine the transpiration and carbon accumulation of Cnidium officinale. Methods and Results: The transpiration of C. officinale was evaluated using weighing lysimeter. The relationship between transpiration and factors such as solar radiation, air temperature, and leaf area was assessed. Transpiration increased as the leaf area increased with the growth stage. Furthermore, daily transpiration per unit leaf area was 0.69 ± 0.16 g·cm-2·day-1 and there were no significant differences in daily transpiration during the cultivation period. The maximum transpiration was 620.6 g m-2·h-1 and diurnal changes in transpiration were highly correlated with solar radiation although the maximum transpiration was observed at the air temperatures of 20℃ - 26℃. The ratio of carbon accumulation to transpiration was 0.12%. Conclusions: Our results indicated that the transpiration of C. officinale is primarily regulated by solar radiation energy on clear days and that 97% of the water is discharged through transpiration for heat dissipation. Therefore, weighing lysimeters can measure transpiration accurately and may be useful in interpreting plant growth.