• Analytical Science and Technology
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• v.28 no.1
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• pp.1-7
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• 2015

4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a tobacco specific nitrosamine found only in tobacco products. The ability to monitor biomarker concentrations is very important in understanding environmental tobacco smoke (ETS). In this study, an efficient and sensitive method for the analysis of NNK in dust was developed and validated using liquid chromatography tandem mass spectrometry. Dust was collected with filter paper soaked in methanol. The standard solution and dust sample were diluted with 100 mM ammonium acetate and extracted using dichloromethane. Our calibration curves ranged from 25 to $10^4pg/mL$. Excellent linearity was obtained with correlation coefficient values between 0.9996 and 1.0000. The limit of detection (LOD) was 5 pg/mL ($S/N{\geq}3$) and the retention time was 10 min. The limit of quantification (LOQ) was 25 pg/mL, and the acceptance criteria was the rate of 98-103% (80-120% at levels up to $3{\times}LOQ$). The coefficient of variations (CV) was 2.8%. Accuracies determined from dust samples spiked with four different levels of NNK racurves ranged that from 25 to 104 pg/mL. Excellent linearity was obtained between 92.1% and 114%. The precision of the method was acceptable (5% of CV). The recovery rates of the whole analytical procedure at low, medium, and high levels were 105.7-116.5% for NNK. The carry-over effects during LC-MS/MS analysis were not observed for NNK. This manuscript summarizes the scientific evidence on the use of markers to measure ETS.

• Journal of the Korean Vacuum Society
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• v.20 no.4
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• pp.288-293
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• 2011

We investigated the effects of piezoelectric field on the electro-absorption characteristics in InGaN/GaN multiple-quantum well (MQW) green light emitting diodes (LED). Double crystal X-ray diffraction measurement was performed to study the crystalline property and indium (In) composition in the MQW active layer. To measure the electro-luminescence and electro-reflectance (ER) spectroscopy, we fabricated the $1{\times}1\;mm^2$ large-area green LED chip. The piezoelectric field inside the LED structure was evaluated from the Vcomp in active layer by the ER spectra. Finally, we analyzed the electro-absorption characteristics of the green LED by using the photo-current spectroscopy.

• Journal of the Korean Applied Science and Technology
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• v.31 no.1
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• pp.74-82
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• 2014

For this research, prepared ammonium dihydrogen phosphate aqueous solution and polyurethane dispersion. Use these resin, this article has been analyzed about change of mechanical properties by increasing amount of ammonium dihydrogen phosphate aqueous solution in polyurethan resin on coated leather and dried film. According to measure data for solvent resistance, DPU(polyurethane dispersion) resin and DPU-AD1, D2, D3(samples of polyurethaneresin with ammonium dihydrogen phosphate aqueous solution) had good property. As known in the results, increase of ammonium dihydrogen phosphate constant did not influence to big change of polyurethane resin properties. As test of tensile strength, DPU had highest tensile characteristic($3.114kg_f/mm^2$) and DPU-AD3 had lowest tensile characteristic($2.510kg_f/mm^2$). As same as tensile characteristic, abrasion test determined DPU(50.5 mg.loss) had highest properties. In elongation case, DPU had best properties(602 %) in this experiment.

• Journal of Korean Society of Forest Science
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• v.113 no.1
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• pp.31-39
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• 2024

This study was performed to determine the effects of Four seedling age classes ageon the characteristics of growth and nutrient uptake in Chamaecyparis obtusa container seedlings. Seedlings (1-1, 2-0, 2-1, and 2-2 seedlings) of C. obtusa grown in containers were harvested to measure specific leaf area, height (H)/root collar diameter (D) ratio, dry mass of aboveground (T)/root dry mass (R) ratio, and seedling quality index of seedlings. The specific leaf area was highest in 1-0 seedlings (30.48 cm² g^-1), whereas it decreased (from 28.62 cm² g^-1 to 23.59 cm² g^-1) with increasing seedling age. The H/D ratio increased with increasing seedling age (from 4.41 in 1-0 seedlings to 8.35 in 2-2 seedlings). The T/R ratio decreased as the seedling age increased (from 4.29 in the 1-0 seedling to 2.13 in the 2-1 seedling). The seedling quality index increased with increasing seedling age (from 0.10 for the 1-0 seedling to 3.06 for the 2-2 seedling). The carbon concentrations of seedling components (leaf, branches, stem, and roots) did not differ significantly with seedling age, whereas the nitrogen concentration of seedling components was the lowest in 2-1 seedlings, as no fertilizer was applied to discourage excessive growth of the seedlings. Phosphorus, potassium, and magnesium concentrations in 2-1 seedling components were not affected by the lack of fertilizer application. These results can be applied to determine the optimum morphological characteristics and nutrient management by seedling age in container- grown C. obtusa.

• Journal of the Korean Society of Radiology
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• v.7 no.3
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• pp.251-257
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• 2013

Spatial dose rates of high dose $^{131}I$ therapy patients were Measured Three dimensional (X, Y, Z) distributions. I have constructed geometrical an aluminum support structure for spatial dose meters placed in 5 different heights, 8 different azimuthal angles, 6 different time interval and distance 100 cm from High dose$^{131}I$ therapy patients. when the height of vertical plane Spatial dose distribution is 100 cm, the Spatial dose rates is max and the error range is low. the vertical plane Spatial dose rates was found to be 71.85 ${\mu}Sv/h$ on the average at a distance of 100 cm, height 100 cm, from the patients 24 hours after $^{131}I$ oral administration. I divided 12 patients into two groups. I have analysed group A (drinking 5 L water) and group B (drinking 3 L water) in order to measure decrease spatial dose rates. I have found the spatial distributions of patient dose rates is $44.9{\pm}7.2$ ${\mu}Sv/h$ in group A and $100.3{\pm}8.1$ ${\mu}Sv/h$ in group B by 24 after $^{131}I$ oral administration. the reduction factor was found to be approximately 54 % through drinking 5 L water during 24 hours.

• Journal of Microbiology
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• v.33 no.3
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• pp.244-251
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• 1995

An extracellular protease of Salmonella schottmulleri was purified from culture filtrate by using 0-75% ammonium sulfate precipitation, DEAE Sepharose Fast Flow ion exchange chromatography, Ultrogel HA chromatography and Sephacryl S-200 HR molecular sieve chromatography. To measure enzyme activity, synthetic dipeptide substrate (CBZ-arg-arg-AFC) with low molecular weight was employed as substrate. The molecular weight of the purified enzyme was approximately 80 kDa when determined by gel filtration on Sephacryl S-200 HR and 73 kDa when estimated by SDS-PAGE. The isoelectric point was 5.45. The activity of the purified enzyme was inhibited by metal chelating agesnts such as EDTA and 1.10-phenanthroline. The divalent cations, such as Ca$\^$2+/, Zn$\^$2+/, Fe$\^$2+/, Mg$\^$2+/ enhanced its activity. These results suggested that it was a metalloprotease. It had a narrow pH optimum of 6.5-7.5 with a maximum at pH 7.0 and a temperature optimum of 40.deg.C. It was stable at least for 1 week at 40.deg.C and maintained its activity for 24 hours at 50.deg.C, but it was rapidly inactivated at 65.deg.C. This protease was shown to be sensitive to sodium 50.deg.C, but it was rapidly inactivated at 65.deg.C. This protease was shown to be sensitive to sodium 50.deg.C, but it was rapidly inactivated at 65.deg.C. This protease was shown to be sensitive to sodium 50.deg.C, but it was rapidly inactivated at 65.deg.C. This protease was shown to be sensitive to sodium dodecyl sulfate (SDS) and was inactivated in a dose-dependent manner. However, it was resistant to Triton X-100 and the activity was enhanced to 32.3% with treatment of 0.025% Triton X-100.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.74.1-74.1
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• 2013

We have performed new narrow-band calcium photometry for Milky Way globular clusters (GCs) and detected multiple red giant branches (RGBs) in some massive GCs. Our new calcium filter was designed to avoid the CN contamination below $3883{\AA}$ and to measure only Ca II H&K lines. The fact that we are detecting multiple RGBs from the new filter is suggesting that they are indeed different in calcium abundance, which can only be produced by supernovae (SNe). Therefore, the presence of the multiple RGBs for the peculiar GCs in the calcium photometry is best understood if the later generation of stars are enhanced in some SNe products. In this talk, we will present our progress in the calcium photometry for the peculiar GCs showing the multiple RGBs.

• Journal of the Korean Society for Precision Engineering
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• v.10 no.3
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• pp.152-160
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• 1993

This paper presents experimental study of the static and dynamic fracture toughness behavior of a A1-6061 aluminum alloy reinforced alumina( .delta. -A1$_{2}$0$_{3}$) whiskers with 5%, 10%, 15% volume fraction. The static fracture tests using three-point bending specimen were performed by UTM25T. And drop weight impact tester performing dynamic fracture tests was used to measure dynamic locads applied to a fatigue-precracked specimes. The oneset of crack initiation was detected uwing a strain gage bonded near a crack tip. The value of static fracture toughness $K_{IC}$ and dynamic fracture toughness $K_{ID}$ were decided on the basis of linear elastic fracture mechanics. The effects of fiber volume fraction and loading on fracture toughness were investigated. The distribution of whiskers, bonding state and fracture interfaces involved in void, fiber pull-out state were investigated by optical microscopy(OM) and scanning electron microscopy(SEM)

• Natural Product Sciences
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• v.10 no.6
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• pp.262-267
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• 2004

A reversed-phase liquid chromatographic method for decursin and decursinol angelate of Angelicae gigantis Radix, an important crude drug in Korean traditional medicine, was developed and validated. Decursin and decursinol angelate, the structure isomer (pyranocoumarin) each other, are the main organic constituents in Angelicae gigantis Radix. This method was developed using a RP-18 column, UV detector at 280 nm and 50% acetonitrile solution containing 0.01 M sodium dodecyl sulfate and 25 mM sodium dihydrogen phosphate (pH 5.0) as the mobile phase. Various validation parameters were included and evaluated satisfactorily. Linearity was established in range 2-75 mg/ml of decursin and decursinol angelate (correlation coefficient = 0.9997 and 0.9995, respectively). This analytical method showed good accuracy (98.1% and 99.5%, respectively). Precision (repeatability) revealed a relative standard deviation value of 1.71% (decursin) and 3.19% (decursinol angelate). For intermediate precision measure the considered variables were equipment and days. A robustness test showing the influence of deferent counter-ion concentration in mobile phase was also performed.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.339-340
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• 1996

In the present study we examine physical characteristics of a thin and rigid magnetic flux tube with a steady flow inside, which is embedded vertically upward in the solar atmosphere. We found from this study that (1) The downward material flow gives rise to a dominant heating in the flux tube which works with the conductive heating in the same direction. However, the upflow flow creates a dominant cooling which works against the conductive heating, resulting in a steeper temperature gradient with a shallower transition region. (2) Since the thickness of the transition region determines the material content in the transition region, a broader transition region of the downflow tube produces a larger differential measure.