• Title/Summary/Keyword: H1*-H2* measure

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Histone Deacetylation Is Involved in Activation of CXCL10 Upon IFNγ Stimulation

  • Guo, Jin-Jun;Li, Qing-ling;Zhang, Jun;Huang, Ai-Long
    • Molecules and Cells
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    • v.22 no.2
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    • pp.163-167
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    • 2006
  • Histone deacetylase (HDAC) activity is commonly associated with transcriptional repression. However, there is also evidence for a function in transcriptional activation. Previous studies have demonstrated a fundamental role of deacetylase activity in $IFN{\alpha}$-responsive gene transcription. In the case of type II IFN ($IFN{\gamma}$) results are controversial: some genes require HDAC activity, while transcription of others is repressed by HDAC. To investigate the effect of HDAC on transcription of an $IFN{\gamma}$-activated gene, real-time PCR was used to measure CXCL10 mRNA in Hela cells stimulated with $IFN{\gamma}$ in the presence or absence of the HDAC inhibitor TSA. Chromatin imunoprecipitation combined with real-time PCR was used to check acetylation of histone H4 and recruitment of the STAT1 complex to the ISRE locus of the CXCL10 gene. Activation of CXCL10 transcription in response to $IFN{\gamma}$ was paralleled by a decrease in histone H4 acetylation and an increase in recruitment of the STAT1 complex to the CXCL10 ISRE locus. The transcription of CXCL10 and histone H4 deacetylation were blocked by TSA, but the latter had no obvious affect on recruitment of the STAT1 complex. Our data indicate that $IFN{\gamma}$ and STAT-dependent gene transcription requires the participation of HDAC, as does the $IFN{\alpha}$-STAT pathway.

Practical and Effective Method for the Solubilization and Characterization of Mammalian ${\beta}$-adrenergic receptor

  • Shin, Chan-Young;Kim, Hee-Jin;Lee, Sang-Bong;Ko, Kwang-Ho
    • Biomolecules & Therapeutics
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    • v.1 no.2
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    • pp.188-195
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    • 1993
  • In order to understand the machanism of action and regulation of ${\beta}$-adrenergic receptor in terms of molecular level, the purification of receptor protein has a fundamental importance. Moreover, species differences among avian, amphibian and mammalian ${\beta}$-adrenergic receptors make it more important to purify mammalian ${\beta}$-adrenergic receptor. Because ${\beta}$-adrenergic receptor is an integral membrane protein, it must be solubilized from the membrane for the purification. The purpose of the present study was to solubilize and characterize the mammalian $\beta$-adrenergic receptor from guinea pig lung in quantities by more efficient and practical method eventually to purify receptor. Guinea pig lung membrane preparation was solubilized by sequential treatment of buffers containing low and high concentration of digitonin which are 0.2 and 1.2% respectively. About 50% of the total receptor pool was released by this double extraction procedure. The $\beta$-adrenoceptors in the digitonin extract were identified using the ${\beta}$-adrenergic antagonist, (-)-[$^3H$]-dihydroalprenolol ([$^3H$]DHA). The solubilized receptor retained all of the essential characteristics of membrane-bound receptor, namely saturability; stereoselectivity; high affinity to ${\beta}$-adrenergic drugs. For the measurement of soluble receptor activity, Sephadex G-50 chromatography method has been widely used. Inspite of its accuracy and wide acceptance, this technique employed troublesome column work which required long time to assay the activity of receptor. We employed another methods to measure receptor activity. When using 0.5% polyethylenimine pretreated GF/B glass fiber filter, filtration technique could be used to measure soluble receptor activity. This technique enabled us to reduce the total amount of time to assay by a factor of 4 as well as to detect soluble receptor. In the present study, we could establish more efficient and practical solubilization method of mammalian $\beta$-adrenergic receptor. The rapidity and high yield of this solubilization scheme, together with the favorable recovery of the receptor activity, are significant steps toward the ultimate purification of the mammalian $\beta$-adrenergic receptor. The result of this study together with more convenient purification method could provide large amount of purified receptor with ease for various research purposes.

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INTELLIGENT VIDEO ENCODING FOR MPEG-1/2 VIDEO

  • Arachchi, H.Kodikara;Fernando, W.A.C.
    • Proceedings of the IEEK Conference
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    • 2002.07a
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    • pp.607-610
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    • 2002
  • Even though digital video coding techniques have been developed very rapidly, rate controlling is still an unsolved problem. This paper presents a new scene adaptive video encoding scheme for MPEG-1 and MPEG-2 video encoders. Degradation of picture quality at scene changes due to poor prediction of global complexity measure is addressed. The proposed scheme determines the picture types adaptively based on scene contents of each video frame. The effective placement of reference and inter-reference frames is studied at sudden scene changes and gradual scene changes respectively. Results show that the proposed scheme demonstrates a significant improvement in performance compared to TM5〔1〕.

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Calibrating the stellar velocity dispersion in near-IR

  • Kang, Wol-Rang;Woo, Jong-Hak
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.52.2-52.2
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    • 2011
  • The correlation between black hole mass and galaxy stellar velocity dispersion gives an important clue on the black hole growth and galaxy evolution. In the case of AGN, however, it is extremely difficult to measure stellar velocity dispersions in the optical spectra since AGN continuum dilutes stellar absorption features. In contrast, stellar velocity dispersions of active galaxies can be measured in the near-IR, where AGN-to-star flux ratio is much smaller, particularly with the laser-guide-star adaptive optics. However, it is crucial to test whether the stellar velocity dispersion measured from the near-IR spectra is consistent with that measured from the optical spectra. Using the TripleSpec at the Palomar 5-m Telescope, we obtained high quality spectra ranging from 1 to 2.4 micron for a sample of 35 nearby galaxies, for which dynamical black hole masses and optical stellar velocity dispersion measurements are available, in order to calibrate the stellar velocity dispersion in the near-IR. In this poster, we present the initial results based on 10 galaxies, with the stellar velocity dispersion measured in the H-band.

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Fabrication of PZT Film by a Single-Step Spin Coating Process

  • Oh, Seung-Min;Kang, Min-Gyu;Do, Young-Ho;Kang, Chong-Yun;Nahm, Sahn;Yoon, Seok-Jin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.02a
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    • pp.193-193
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    • 2011
  • To obtain ceramic films, the sol-gel coating technique has been broadly used with heat treatment, but crack formation tend to occur during heat treatment in thick sol-gel films. We prepared PZT thin films by sol-gel method with single-step spin coating process. The PZT solution have been synthesized using lead acetate ($Pb(CH_3COO)_2$), zirconium acetylacetonate ($Zr(OC_3H_7^n)_4$), and titanium diisopropoxide bis(acetylacetonate) 75wt% in isopropanol ($Ti(OC_3H_7^i)_2(OC_3H_7^n)_2$) as starting materials and n-propanol was selected as a solvent. The poly(vynilpyrrolidone) (PVP) was added with 0, 0.25, 0.5, 0.75, and 1 molar ratios to control viscosity of solution. We investigated influence of the viscosity on thickness, microstructure, and electrical properties of final PZT films. Thermo-gravimetric analysis and differential scanning calorimeter (TGA/DSC) was carried out from room temperature to $800^{\circ}C$ in order to measure pyrolysis temperature. Structural characteristics were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Ferroelectric and dielectric properties were measured by RT66A (Radiant) and impedance analyzer (Agilent), respectively. The thicknesses of PZT films depended on incorporation of an excess amount of PVP. Finally, we obtained PZT films of good quality without crack formation via single-step spin coating.

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Development of a Method for Detecting Iodide and Chloride Ions in Infant Formula using an Ion Selectivity Electrode (이온선택성 전극법을 이용한 조제분유중의 염소 및 요오드 이온함량 분석방법 연구)

  • Ko, Jin-Hyouk;Kwak, Byung-Man;Ahn, Jang-Hyuk;Jeon, Tae-Hong
    • Food Science of Animal Resources
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    • v.28 no.3
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    • pp.301-305
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    • 2008
  • This study was carried out to develop an accurate and rapid analysis method to measure the contents of iodide and chloride in infant formula using an ion selectivity electrode. The infant formula SRM 1846 (Standard Reference Material 1846, NIST, USA) was used as a CRM (Certified reference material). Samples were dissolved in water with 3% acetic acid and filtered through filter paper and adjusted to pH 7.0 with 0.1N NaOH. At pH 7.0, the iodide content of the CRM was $1.04{\pm}0.03\;mg/kg$ compared to the specification of $1.11{\pm}0.1\;mg/kg$ of CRM, and the recovery was $93.7{\pm}2.7%$. The chloride content was $5114.3{\pm}240\;mg/kg$ compared to the specification of $4,920{\pm}300mg/kg$ of CRM, and the recovery was $103.9{\pm}4.9%$. Ion selectivity electrodes could be successful1y used to determine the concentration of iodide and chloride ions in infant formula by a simple and rapid pretreatment of sample matrixes.

Comparison of Spray Angles of Multihole Port Fuel Gasoline Injector with Different Measuring Methods (측정방법에 따른 흡기포트 분사식 다공 가솔린인젝터의 분무각 비교)

  • Kim, J.H.;Rhim, J.H.;No, S.Y.;Moon, B.S.
    • Journal of ILASS-Korea
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    • v.5 no.3
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    • pp.17-26
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    • 2000
  • The main parameter commonly used to evaluate spray distribution is spray angle. Spray angle is important because it influences the axial and radial distribution of the fuel. Spray angles were measured and compared for the two non-air assisted injectors such as 2hole-2stream 4hole-1stream injectors used for port fuel injection gasoline engines with n-heptane as a fuel by three different measuring techniques, i.e., digital image processing, shadowgraphy, and spray patternator, respectively. Fuel was injected with the injection pressures of 0.2-0.35 MPa into the room temperature and atmospheric pressure environment. In digital image processing approach, the selection of the transmittance level is critical to obtain the edge of spray and hence to measure the spray angle. From the measurement results by the shadowgraphy technique, it is dear that the spray angle is varied during the spray injection period. The measurement results from spray patternator show that the different spray angles exist in different region. Spray angle increases with the increase in the injection pressure. it is suggested that the spray angle and stream separated angle should be specified when spray is characterized for 2hole-2stream injector, because spray angle is much different though stream separated angle is same. It was also considerably affected by the measurement techniques introduced in this experimental work. However, the optimal axial distance for measuring the spray angle seems to be at least 60-80 mm from the injector tip for two non-air assisted injectors.

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Analysis of the axle load of an agricultural tractor during plow tillage and harrowing

  • Hong, Soon-Jung;Park, Seung-Je;Kim, Wan-Soo;Kim, Yong-Joo;Park, Seong-un
    • Korean Journal of Agricultural Science
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    • v.43 no.4
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    • pp.665-669
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    • 2016
  • Analysis of the load on the tractor during field operations is critical for the optimal design of the tractor. The purpose of this study was to do a load analysis of an agricultural tractor during plowing and harrowing. First, a load measurement system was developed and installed in a 71 kW agricultural tractor. Strain-gauges with a telemetry system were installed in the shaft located between the axles and the wheels, and used to measure the torque of the four driving axles. Second, field experiments were conducted for two types of field operations (plowing, harrowing), each at two gear levels (M2, M3). Third, load analysis was conducted according to field operation and gear level. At M2 gear selection for plowing, the maximum, minimum, and average (S. D.) torque values were 13,141 Nm; 4,381 Nm; and 6,971 Nm (${\pm}397.8Nm$, respectively). For harrowing, at M2 gear selection, torque values were, 14,504 Nm; 1,963 Nm; and 6,774 Nm (${\pm}459.4Nm$, respectively). At M3 gear selection for plowing, the maximum, minimum, and average (S. D.) torque values were,17,098 Nm; 6,275 Nm; and 8,509 Nm (${\pm}462.4Nm$, respectively). For harrowing at M3 gear selection, maximum, minimum, and average (S. D.) torque values were, 20,266 Nm; 2,745 Nm; and 9,968 Nm (${\pm}493.2$). The working speed of the tractor increased by approximately 143% when shifted from M2 (7.2 km/h) to M3 (10.3 km/h); while during plow tillage and harrowing, the load of the tractor increased approximately 1.2 times and 1.5 times, respectively.

The Antioxidative Activities of Torreya nucifera Seed Extracts (비자(Torreya nucifera) 추출물의 생리활성)

  • Jeon, Ho-Sung;Lee, Yang-Suk;Kim, Nam-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.1
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    • pp.1-8
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    • 2009
  • This study was investigated to analyze the contents of flavonoid and polyphenol compounds, and inhibitory activities of tyrosinase and antioxidation to measure physiological effect of reflux water extraction (WE), reflux ethanol extraction (EE) and hot water extract under high pressure (HWE) of Torreya nucifera seed. HWE yields the highest contents of flavonoid compounds (176.34 mg/g) and polyphenol compounds (112.95 mg/g). The tyrosinase inhibitory rates were $5.62{\sim}28.71%$ at 2.0 mg/mL and HWE showed the highest inhibition rate. The nitrite scavenging abilities of all extracts were over 90% at pH 1.2 and 3.0 at the concentration of 2.0 mg/mL. The superoxide dismutase (SOD)-like activities of HWE was the highest value of 33.58%. The electron donating abilities (EDA) were $66.46{\sim}89.72%$ and HWE was the highest when the extracts were tested at 0.1 mg/mL. The EDA of all extracts were decreased with an increment of the extracts concentrations. The xanthine oxidase inhibitory rate of HWE was the highest value of 89.29% at the concentration of 2.0 mg/mL and the WE and HWE were over 75% rate of xanthine oxidase inhibition at 0.5 mg/mL.

A Forward Speed Control of Head-feed Combine Using Continuously Variable V-belt Transmission -Combine Load Characteristics- (V-벨트 무단변속기(無斷變速機)를 이용(利用)한 자탈형(自脫型) 콤바인의 주행속도(走行速度) 제어(制御)(I))

  • Choi, K.H.;Ryu, K.H.;Cho, Y.K.;Park, P.K.
    • Journal of Biosystems Engineering
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    • v.16 no.2
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    • pp.124-132
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    • 1991
  • This study was carried out to obtain the information needed in the development of forward speed control system and the improvement of combine performance. The effects of variety, grain moisture content and forward speed on the combine load characteristics were investigated through experiments. The results of this study are summarized as follows. 1. A data acquisition system was developed to measure the engine speed and the torques and speeds of the threshing cylinder, dean-grain auger and tailings-return auger. The system consisted of transducers, signal conditioner, interface board and microcomputer. The system accuracy is better than ${\pm}2.3%$ full scale. 2. Linear regression equations were obtained for the torque, speed and power requirement of threshing cylinder for different paddy varieties, grain moisture contents and feed rates. 3. The maximum value of relative frequency for threshing cylinder torque decreased as the increase in feed rate and moisture content. The range of torque fluctuation was 1.2~3.7 and 1.2~1.9 times the average and maximum torque, respectively. The maximum value of power spectrum density (PSD) appeared to be about 11 Hz regardless of paddy variety, grain moisture content and feed rate. 4. The speed of tailings return thrower decreased rapidly at below 900rpm, and it fell to near zero about 3 seconds after that time. When the travelling of combine harvester was stopped immediately after sensing the overload, it took about 7 seconds for a full recovery of the no-load speed of tailings return thrower.

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