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Influence of Municipal Abattoir Conditions and Animal-related Factors on Avoidance-related Behaviour, Bleeding Times at Slaughter and the Quality of Lamb Meat

  • Njisane, Yonela Z.;Muchenje, Voster
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.10
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    • pp.1496-1503
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    • 2013
  • The objective of this study was to determine the effect of municipal abattoir conditions and animal factors on avoidance-related behaviour (AB) of sheep at slaughter, bleeding times (BT) and mutton quality. The behaviour of 66 castrates and 19 ewes of different age categories was observed at three stages of slaughter. Higher behaviour scores indicated higher levels of AB. Time intervals between the start of blood flow and the time the flow changed from a constant stream into drips were recorded as BT. Thirty two meat samples were obtained to measure quality variables. These were colour ($L^*$, $a^*$ and $b^*$), $pH_{24}$, temperature, cooking loss (CL) and tenderness. Correlations were determined between BT and meat quality variables. Animal behaviour at slaughter differed with breed, gender and age group. Avoidance behaviour was higher in the Dorper breed than in both the Merino breed and their crosses. It was also higher in younger (<10 months) lambs than in older sheep. Castrates were more aggressive or in panic than ewes. Castrates had longer ($72.6{\pm}0.53s$) BT than the ewes ($63.6{\pm}2.82s$). Ewes had higher CL ($39.8{\pm}1.04%$) values than castrates ($35.1{\pm}0.95%$). Meat from castrates was tougher ($32.6{\pm}1.95N$) than the meat from ewes ($24.3{\pm}1.16N$). There were no significant correlations obtained between BT and meat quality variables. It can therefore be concluded that abattoir conditions, breed, age and gender had an effect on AB at slaughter. Gender had an effect on BT and mutton quality.

Characterization of Hydrogen Gas Sensitivity of TiO2 Thin Films with Electron Beam Irradiation (전자빔 열처리에 따른 TiO2 박막의 수소가스 검출 특성 연구)

  • Heo, S.B.;Lee, H.M.;Jung, C.W.;Kim, S.K.;Lee, Y.J.;Kim, Y.S.;You, Y.Z.;Kim, D.
    • Journal of the Korean Society for Heat Treatment
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    • v.24 no.1
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    • pp.31-36
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    • 2011
  • $TiO_2$ films were deposited on a glass substrate with RF magnetron sputtering and then surface of $TiO_2$ films were electron beam irradiated in a vacuum condition to investigate the effect of electron bombardment on the thin film crystallization, surface roughness and gas sensitivity for hydrogen. $TiO_2$ films that electron beam irradiated at 450eV were amorphous phase, while the films irradiated at 900 eV show the anatase (101) diffraction peak in XRD pattern. AFM measurements show that the roughness is depend on the electron irradiation energy. As increase the hydrogen gas concentration and operation temperature, the gas sensitivity of $TiO_2$ and $TiO_2$/ZnO films is increased proportionally and $TiO_2$ films that electron beam irradiated at 900 eV show the higher sensitivity than the films were irradiated at 450eV. From the XRD pattern and AFM observation, it is supposed that the crystallization and rough surface promote the hydrogen gas sensitivity of $TiO_2$ films.

Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry

  • Park C. S.;Li Z. H.;Sung N. D.;Jin D. I.;Cong P. Q.;Kim E. S.;Song E. S.;Yi Y. J.
    • Reproductive and Developmental Biology
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    • v.29 no.4
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    • pp.253-257
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    • 2005
  • This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than $80\%$ of fresh sperm washed with mTLP-PVA medium at $20^{\circ}C$ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane $(36.4\~46.9\%)$ and nonfunctional mitochondrion $(55.1\~71.1\%)$ in the mTLP-PVA and BTS washing media at $20^{\circ}C$. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at $4^{\circ}C$ washing temperature than at $20^{\circ}C$ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

Characterization of Canthaxanthin Isomers Isolated from a New Soil Dietzia sp. and Their Antioxidant Activities

  • Venugopalan, Vijayalatha;Tripathi, Subhash K.;Nahar, Pradip;Saradhi, P. Pardha;Das, Rakha H.;Gautam, Hemant K.
    • Journal of Microbiology and Biotechnology
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    • v.23 no.2
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    • pp.237-245
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    • 2013
  • Canthaxanthin (cx) is a potent antioxidant that is chemically synthesized at the industrial scale and has imperative applications in the cosmetic and feed industries. An orange pigmented mesophilic bacterium, designated as K44, was isolated from soil samples of Kargil, India. Biochemical tests, 16S rRNA gene sequencing, and FAME analysis of the bacterium indicated it to belong in the genus Dietzia and is distinct from human isolates. The strain showed 98% 16S rRNA gene sequence homology with Dietzia maris DSM 43102. High-performance liquid chromatography profile of the pigments isolated from K44 showed two major peaks absorbing at 465.3 and 475 nm. The liquid chromatography-mass spectrometry (LC-MS) analysis of both these peaks revealed their m/z to be 564. The molecular weights, LC-MS/MS fragmentation patterns, and ${\lambda}_{max}$ of these fractions corresponded to all-trans- (475 nm) and 9-cis-(465.3 nm) cx isomers. The antioxidant activities of cis- and trans-cx isomers isolated from this bacterium were found to differ, where the cis-isomer showed higher free radical, superoxide radical, and reactive oxygen species scavenging activities than the alltrans- isomer, suggesting that 9-cis-cx is more effective as an antioxidant than the all-trans-cx.

THE CHANGE IN SURFACE CONVERSION AND DISCOLORATION IN DENTAL RESTORATIVE COMPOSITE RESIN UNDER DIFFERENT POLISHING METHODS; THE CORRELATION BETWEEN SURFACE CONVERSION AND SURFACE DISCOLORATION (수복용복합레진의 표면처리방법에 따른 표면중합률 및 변색정도의 변화와 그 상관관계에 대한 연구)

  • Park, Sung-Ho;Noh, Byung-Duk;Kim, Mo-Ran;Ahn, Hyun-Jung
    • Restorative Dentistry and Endodontics
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    • v.25 no.3
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    • pp.482-486
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    • 2000
  • The purpose of the present study was first, to evaluate the relationship between composite surface conversion and surface discoloration, second, to know if there was difference in surface discoloration between celluloid-strip-finished composite surface and polished surface. In addition, the discoloration of composite surface was also evaluated with visual inspection or digital camera with high resolution monitor, Z100, Tetric Ceram, Spectrum, and Aelitfil were used. The composite surfaces were celluloid-strip finished (group 1), polished (group 2), celluloid-strip finished under nitrogen gas purging (group 3) or only light cured without finishing or polishing under nitrogen gas purging (group 4). The microhardness of each samples were also measured in each group. The samples of each group were also divided into 4 subgroup whether they were immediately placed in disclosing solution (0.2% Elythrosin, pH 7.0) (subgroup1), 1 day after light curing(subgroup 2), 3day after light curing(subgroup 3) or 7 day after light curing(subgroup 4). The computer controlled spectrophotometer was used to determine CIELAB coordinates ($L^*$, $a^*$, $b^*$). The amounts of color difference were compared. The results were as follows; 1. There was no difference in discoloration between celluloid strip finished composite surface and polished surface. 2. The samples discolored more when they were placed in disclosing solution immediately after polymerization than other groups. 3. When the samples were light cured under nitrogen gas purging and without polishing process, they discolored more than other groups even though they showed higher micro hardness. 4. With visual inspection or digital camera, only a limited information was available in detecting composite surface discoloration.

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Underwater E-plane Attenuation Model of Omnidirectional Antenna Using Half Power Beam Width (HPBW) (반전력빔폭을 이용한 전방향성 안테나의 수중 환경 수직 평면 감쇠 모델)

  • Kwak, Kyungmin;Park, Daegil;Kim, Younghyeon;Chung, Wan Kyun;Kim, Jinhyun
    • Journal of Institute of Control, Robotics and Systems
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    • v.21 no.11
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    • pp.1050-1056
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    • 2015
  • In this paper, we use the characteristics of electromagnetic waves underwater attenuation for estimating linear distance between a transmitting node and receiving node, and research underwater vertical plane attenuation model for constructing the underwater localization system. The underwater localization of 2 dimensional with the plane attenuation model in the horizontal plane (H-plane) was proposed previous research. But for the 3 dimensional underwater localization, the additional vertical plane (E-plane) model should be considered. Because the horizontal plane of omnidirectional antenna has the same attenuation tendency in x-y plane according to the distance, whereas in vertical plane shows an irregular pattern in x-z plane. For that reason, in the vertical plane environment, the attenuation should be changed by the position and inclination. Hence, in this paper the distance and angle between transmitting and receiving node are defined using spherical coordinate system and derive an antenna gain pattern using half power beam width (HPBW). The HPBW is called a term which defines antenna's performance between isotropic and other antennas. This paper derives omnidirectional antenna's maximum gain and attenuation pattern model and define vertical plane's gain pattern model using HPBW. Finally, experimental verifications for the proposed underwater vertical plane's attenuation model was executed.

THE BIMA PROJECT: O-C DIAGRAMS OF ECLIPSING BINARY SYSTEMS

  • HAANS, G.K.;RAMADHAN, D.G.;AKHYAR, S.;AZALIAH, R.;SUHERLI, J.;IRAWATI, P.;SAROTSAKULCHAI, T.;ARIFIN, Z.M.;RICHICHI, A.;MALASAN, H.L.;SOONTHORNTHUM, B.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.205-209
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    • 2015
  • The Eclipsing Binaries Minima (BIMA) Monitoring Project is a CCD-based photometric observational program initiated by Bosscha Observatory - Lembang, Indonesia in June 2012. Since December 2012 the National Astronomical Research Institute of Thailand (NARIT) has joined the BIMA Project as the main partner. This project aims to build an open-database of eclipsing binary minima and to establish the orbital period of each system and its variations. The project is conducted on the basis of multisite monitoring observations of eclipsing binaries with magnitudes less than 19 mag. Differential photometry methods have been applied throughout the observations. Data reduction was performed using IRAF. The observations were carried out in BVRI bands using three different small telescopes situated in Indonesia, Thailand, and Chile. Computer programs have been developed for calculating the time of minima. To date, more than 140 eclipsing binaries have been observed. From them 71 minima have been determined. We present and discuss the O-C diagrams for some eclipsing binary systems.

Genetic Analysis and Serological Detection of Novel O-Antigen Gene Clusters of Plesiomonas shigelloides

  • Wang, Xiaochen;Xi, Daoyi;Li, Yuehua;Yan, Junxiang;Zhang, Jingyun;Guo, Xi;Cao, Boyang
    • Journal of Microbiology and Biotechnology
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    • v.31 no.4
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    • pp.520-528
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    • 2021
  • Plesiomonas shigelloides, a member of the family Vibrionaceae, is a gram-negative, rod-shaped, facultative anaerobic bacterium with flagella. P. shigelloides has been isolated from such sources as freshwater, surface water, and many wild and domestic animals. P. shigelloides contains 102 O-antigens and 51 H-antigens. The diversity of O-antigen gene clusters is relatively poorly understood. In addition to O1 and O17 reported by other laboratories, and the 12 O serogroups (O2, O10, O12, O23, O25, O26, O32, O33, O34, O66, O75, and O76) reported previously by us, in the present study, nine new P. shigelloides serogroups (O8, O17, O18, O37, O38, O39, O44, O45, and O61) were sequenced and annotated. The genes for the O-antigens of these nine groups are clustered together in the chromosome between rep and aqpZ. Only O38 possesses the wzm and wzt genes for the synthesis and translocation of O-antigens via the ATP-binding cassette (ABC) transporter pathway; the other eight use the Wzx/Wzy pathway. Phylogenetic analysis using wzx and wzy showed that both genes are diversified. Among the nine new P. shigelloides serogroups, eight use wzx/wzy genes as targets. In addition, we developed an O-antigen-specific PCR assay to detect these nine distinct serogroups with no cross reactions among them.

Assessment of the physico-chemical quality and extent of algal proliferation in water from an impounding reservoir prone to eutrophication

  • Ballah, Mohun;Bhoyroo, Vishwakalyan;Neetoo, Hudaa
    • Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.22-30
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    • 2019
  • Background: Piton du Milieu (PdM) impounding reservoir is suspected to be eutrophic based on the elevated level of orthophosphate and nitrate. Water supplies from three adjacent rivers are primarily thought to contribute to the nutrient enrichment of the reservoir. It is also suspected that there is leaching of orthophosphate, nitrate and organic matter into the rivers during rainfall events and also as a result of anthropogenic activities within the catchment area. The aim of this study was to ascertain the impact of nutrient loading on the water quality of PdM water and on the population of freshwater microalgae in the reservoir. The enumeration and identification of algae from PdM were performed by differential interference contrast microscopy. Dissolved oxygen (DO) and pH were determined by electrometric methods, whereas nutrient levels, silica and total organic carbon (TOC) were determined by instrumentation techniques. Results: Annual mean orthophosphate, nitrate and total organic carbon input from the three feeders within the catchment area of PdM reached levels as high as 0.09 mg/L, 0.4 mg/L and 2.62 ppm respectively. Over a 12-month period, mean TOC concentration in the reservoir was 2.32 ppm while the mean algal cell count was 4601 cells/mL. The dominant algal species identified were Oscillatoria, Cyclotella, Navicula and Cosmarium. Conclusion: This study highlights the trophic state of the reservoir water and clearly points to the need for constant monitoring in order to avoid the occurrence of an impending harmful algal bloom.

SEOUL NATIONAL UNIVERSITY AGN MONITORING PROJECT. I. STRATEGY AND SAMPLE

  • Woo, Jong-Hak;Son, Donghoon;Gallo, Elena;Hodges-Kluck, Edmund;Jeon, Yiseul;Shin, Jaejin;Bae, Hyun-Jin;Cho, Hojin;Cho, Wanjin;Kang, Daeun;Kang, Wonseok;Karouzos, Marios;Kim, Minjin;Kim, Taewoo;Le, Huynh Anh N.;Park, Daeseong;Park, Songyoun;Rakshit, Suvendu;Sung, Hyun-il
    • Journal of The Korean Astronomical Society
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    • v.52 no.4
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    • pp.109-119
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    • 2019
  • While the reverberation mapping technique is the best available method for measuring black hole mass in active galactic nuclei (AGNs) beyond the local volume, this method has been mainly applied to relatively low-to-moderate luminosity AGNs at low redshift. We present the strategy of the Seoul National University AGN Monitoring Project, which aims at measuring the time delay of the $H{\beta}$ line emission with respect to AGN continuum, using a sample of relatively high luminosity AGNs out to redshift z ~ 0.5. We present simulated cross correlation results based on a number of mock light curves, in order to optimally determine monitoring duration and cadence. We describe our campaign strategy based on the simulation results and the availability of observing facilities. We present the sample selection, and the properties of the selected 100 AGNs, including the optical luminosity, expected time lag, black hole mass, and Eddington ratio.