• Title/Summary/Keyword: H.332

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Adsorption and Movement of Fenoxaprop-P-ethyl in Soils (토양중 fenoxaprop-P-ethyl의 흡착성 및 이동성)

  • Han, Soo-Gon;Ahn, Byung-Koo;Moon, Young-Hee
    • Korean Journal of Weed Science
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    • v.18 no.4
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    • pp.325-332
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    • 1998
  • This study was carried out to investigate the adsorption and the movement of herbicide fenoxaprop-P-ethyl in the silty clay soil(SiC) and the sandy loam soil(SL). Fifteen percent of the added herbicide was adsorbed within 30 min after shaking, and a quasi-equilibrium was reached after 8 to 14 h. The time required for 50% adsorption was 15.8 h in the SiC and 19.3 h in the SL. The equilibrium adsorption isotherm was followed by the Freundlich equation and the Kd was 3.86 in the SiC and 2.32 in the SL. The herbicide in the soil columns flooded with 3 cm water depth and eluted at 0.8 cm/day was leached to 6 cm and 8 cm depth at 7 and 21 days after the treatment, respectively. However, the movement was widened with increased amount of leaching water. The herbicide in the field soils was moved up to 6 cm and 8 cm depth at 14 and 56 days after the treatment, respectively. However, the large amount of the applied herbicide was distributed in 0~2 cm profile in all of the soils examined. Half-life of the chemical in soils was shorter than 7 days and the time to 90% degradation was about 4 weeks. The results indicate that the herbicide has relatively small mobility and short persistence.

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Isolation and Characterization of a Paenibacillus incheonensis YK5 with Antimicrobial Activity aginst MRSA (항MRSA 활성을 보이는 Paenibacillus incheonensis YK5의 분리 및 특성)

  • Yoon, Young-Jun;Kim, Hye-Yoong;Lee, Tae-Soo;Kim, Jung-Wan
    • Korean Journal of Microbiology
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    • v.44 no.4
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    • pp.326-332
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    • 2008
  • Various bacteria were isolated from Korean soil samples based on their capability inhibiting the growth of MRSA strains. Among them, strain YK5 with the highest activity was a Gram positive sporulative bacillus with motility. It did not produce indole and no acid was formed from mannitol by the bacterium. The 16S rRNA sequence of the strain showed $95{\sim}98%$ homology with those of Paenibacillus spp.. The bacterial isolate shared the highest homology with that of P. elgii (98%), but was named as Paenibacillus incheonensis YK5 due to differences in physiological properties. Butanol extract of the P. incheonensis YK5 culture grown in SST medium at $37^{\circ}C$ for 96 hr showed a broad antimicrobial activity against Gram-positive (MRSA and Streptococcus pneumoniae) and negative (Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Escherichia coli, Klebsiella pneumoniae) pathogenic bacteria and fungi (Cryptococcus neoformans and Trichophyton). The antimicrobial activity in the crude extract was stable in a broad range of temperature and pH, $20{\sim}100^{\circ}C$ and $3.0{\sim}6.0$, respectively. Therefore, the antimicrobial activity of P. incheonesis YK5 had potential as a novel antibiotics for pathogens including MRSA.

Study of characteristics of $AgGaS_2$/GaAs epilayer by hot wall epitaxy (HWE 방법에 의한 $AgGaS_2$/GaAs epilayer 성장과 특성)

  • Hong, K.J.;Jeong, J.W.;Bang, J.J.;Jin, Y.M.;Kim, S.H.;Yoe, H.S.;Yang, H.J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.08a
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    • pp.84-91
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    • 2002
  • The stochiometric composition of $AgGaS_2$/GaAs polycrystal source materials for the $AgGaS_2$/GaAs epilayer was prepared from horizontal furnace. From the extrapolation method of X-ray diffraction patterns it was found that the polycrystal $AgGaS_2$/GaAs has tetragonal structure of which lattice constant an and Co were 5.756 $\AA$ and 10.305 $\AA$, respectively. $AgGaS_2$/GaAs epilayer was deposited on throughly etched GaAs(100) substrate from mixed crystal $AgGaS_2$/GaAs by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were $590^{\circ}C$ and $440^{\circ}C$ respectively. The crystallinity of the grown $AgGaS_2$/GaAs epilayer was investigated by the DCRC (double crystal X-ray diffraction rocking curve). The optical energy gaps were found to be 2.61 eV for $AgGaS_2$/GaAs epilayer at room temperature. The temperature dependence of the photocurrent peak energy is well explained by the Varshni equation, then the constants in the Varshni equation are given by $\alpha=8.695{\times}10^{-4}$ eV/K, and $\beta=332K$. From the photocurrent spectra by illumination of polarized light of the $AgGaS_2$/GaAs epilayer, we have found that crystal field splitting ${\Delta}Cr$ was 0.28 eV at 20 K. From the PL spectra at 20 K, the peaks corresponding to free and bound excitons and a broad emission band due to D-A pairs are identified. The binding energy of the free excitons are determined to be 0.2676 eV and 0.2430 eV and the dissociation energy of the bound excitons to be 0.4695 eV.

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Feeding Habits of Fat Greenling Hexagrammos otakii off the Incheon Coast of Korea (인천 연안에 출현하는 쥐노래미(Hexagrammos otakii)의 식성)

  • Tae-Hyoung Roh;Byeong-Il Youn;Su-Jin Koh;Kyeong-Ho Han;Seung-Hwan Lee
    • Korean Journal of Ichthyology
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    • v.35 no.4
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    • pp.325-332
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    • 2023
  • The feeding habits of the Fat Greenling Hexagrammos otakii were studied using 376 specimens (17.4~41.4 cm in total length) collected off the Incheon coast, Korea between March 2020 and February 2021. H. otakii ate mainly gastropoda and teleostei, incuding caridea, brachyura, polychaeta, cephalopoda and amphipoda. The diet composition of H. otakii showed changes in season. H. otakii ate mainly cephalopoda in spring and ate mainly polychaeta in summer. The teleostei feeding rate was highest in autumn, whereas the proportion of gastropoda was higher in winter than in other seasons. The mean number of preys per stomach (mN/ST, One-way ANOVA, F=2.026, P>0.05) and mean weight of preys per stomach (mW/ST, One-way ANOVA, F=1.075, P>0.05) didn't show a statistically significant difference among size classes.

Quality Characteristics of Makgeolli using Angelica gigas Nakai Water Extracts (당귀 열수 추출물을 이용한 막걸리의 품질 특성)

  • Lee, Jang Mi;Lee, Ha Na;Chang, Yun Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.23 no.3
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    • pp.332-340
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    • 2013
  • The aim of this study was to investigate the quality characteristics of makgeolli that was produced by using different percentages (control, 3%, 5%, 7%, 9% by rice weight) of Angelica gigas Nakai water extracts during fermentation at $25^{\circ}C$ for 7 days. Yeast cell count in makgeolli steadily increased and showed the highest value at day 4 of fermentation, since then, the count decreased consistently. The final alcohol contents were higher than 15% in all samples. Soluble contents and antioxidation activity were significantly increased in percentage as Angelica water extracts increased (p<0.05). As the amount of Angelica water extracts increased, total polyphenol contents increased as well. Soluble contents and total polyphenol contents were increased according to the fermentation period. Total acidity increased up to day 3, but slowly decreased afterwards. However, the pH rapidly decreased by day 3, but then increased steadily. Reducing sugar amount was increased sharply by day 2 of fermentation and then, diminished gradually. Hunter's color value was not significantly different among samples however, they were significantly different between day 2 samples of fermentation and day 3 or more samples (p<0.05). In sensory evaluations, the sample produced using 5% Angelica water extracts received the highest score in herbal flavor, herbal taste, sourness, sweetness, and overall acceptance. Makgeolli using Angelica gigas Nakai water extracts showed excellent characteristics in antioxidative activity and higher total polyphenol content. Makgeolli produced using 5% Angelica water extracts received the highest score in sensory evaluation. Therefore, makgeolli produced using 5% Angelica water extracts was considered to be the most suitable for manufacturing makgeolli.

Partition of Amino Acids Requirement for Maintenance and Growth of Broilers I. Lysine

  • Kim, J.H.;Cho, W.T.;Yang, C.J.;Shin, I.S.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.2
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    • pp.178-184
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    • 1997
  • Purified diets containing five graded levels of lysine were fed to a total of 125 growing chicks (25 chicks per treatment) to evaluate lysine requirements for growth and maintenance. A model was developed to separate lysine requirement for maintenance from requirement for growth. Based on weight gain response, the daily lysine requirement for growth was 12.06 mg/g gain and the daily lysine requirement for maintenance was 0.332 times metabolic body size ($W^{0.75}$). Similarly, the lysine requirement for growth was 0.457 mg/mg nitrogen gain and the daily lysine requirement for maintenance was 0.344 times metabolic body size. The plateau of plasma lysine concentration was reached at 354.75 mg intake/day. The total lysine requirement was 414.27 mg/day or 1.0% of the diet, 420.11 mg/day or 1.01% of the diet based on weight gain response and N gain response, respectively. Previous lysine requirements for growing chicks of 1-28 days old were in close agreement with these estimates. As a percentage of protein, lysine requirement was calculated to be 7.3% and the reported lysine content of chick muscle crude protein of 7.46% was closely related.

Antioxidant Activities of Various Extracts from Korean Yam (Dioscorea batatas DECNE.)

  • Duan, Yishan;Kim, Gyeong-Hwuii;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.324-332
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    • 2016
  • The objective of this study was to investigate the antioxidant activity of Korean yam (Dioscorea batatas $D_{ECNE.}$) by different extraction solvents including 70% methanol, 70% ethanol and chloroform-methanol mixture (CM, 2:1, v/v). Raw yam was analyzed for its color property, total phenol content and antioxidant activity. Yam possessed high $L^*$ value and $H^{\circ}$ value, which were $81.64{\pm}2.59$ and $83.36{\pm}0.15$, respectively. Raw yam was found to have great antioxidant activity evaluated through ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, and ferric reducing antioxidant power. Total phenol contents of various extracts from raw yam increased in the following order: 70% methanol extract ($37.62{\pm}0.88mg\;CAE/g$), 70% ethanol extract ($43.38{\pm}0.66mg\;CAE/g$) and CM extract ($67.17{\pm}0.12mg\;CAE/g$), respectively. The same trend was also could be found in the ABTS radical scavenging activity, DPPH radical scavenging activity and ferric reducing antioxidant power assays. These results implied that Korean yam might play an important role in antioxidation and serve as the bio-health functional food to take a good part in prevention of human diseases and aging.

Expression of Glucose Isomerase Gene from Bacillus licheniformis in Escherichia coli. (Bacillus licheniformis 포도당 이성화 효소 유전자의 Excherichia coli에 발현)

  • 신명교;고영희
    • Korean Journal of Microbiology
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    • v.23 no.2
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    • pp.138-146
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    • 1985
  • A Bacillus licheniformis ATCC31667 gene coding for a glucose isomerase has been cloned and expressed in glucose isomerase negative mutant of Escherichia coli. A recombinant plasmid, constructed by ligation of a EcoRI fragment of B.licheniformis chromosomal DNA to vector plasmid pBR322, was expressed glucose isomerase positive in E.coli LE392-6 with growth on minimal medium containing xylose as a sole carbon source. This recombinant plasmid, designated pBGI6, had the insery of 4.1Kb of Bacillus gene in EcoRI site, and restriction map of the plasmid was established. The plasmid pBG16 was very stable after 10days of serial transfer to a fresh medium. The activity of glucose isomerase from the transformed cell containing pBGI6 was increased about 20 fold than its wild type of host.

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Selection of Molecular Biomarkers Relevant to Abnormal Behaviors of Medaka Fish (Oryzias latipes) Caused by Diazinon (다이아지논에 의해 야기된 송사리의 이상행동 연관 분자생물지표의 선발)

  • Koh, Sung-Cheol;Shin, Sung-Woo;Cho, Hyun-Duk;Chon, Tae-Soo;Kim, Jong-Sang;Lee, Sung-Kyu
    • Environmental Analysis Health and Toxicology
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    • v.24 no.4
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    • pp.321-332
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    • 2009
  • 본 연구의 목적은 다이아지논(Diazinon; O, O-diethyl O-[6-methyl-2 (1-methylethyl)-4-pyrimidinyl] phosphorothioate)에 노출된 모델 생물체(송사리)의 행동변화와 관련된 분자생물학적 기전 규명을 통하여 비정상적 행동의 모니터링을 위한 생물지표(biomarker)를 개발하는데 있다. 이를 위해 우선 suppression subtractive hybridization (SSH) 및 DNA microarray 기법을 활용하여 다양한 유전자를 스크리닝하였다. 다이아지논에 노출시킨 송사리에서 발현의 차이가 나는 상향 조절된 유전자 97개 (알려지지 않은 유전자 27개 포함)와 하향 조절된 유전자 99개 (알려지지 않은 유전자 60개 포함)를 동정 하였고 이들 중 이상행동과 관련되는 것으로 보이는 유전자 10개 (상향조절 5개, 하향조절 5개)를 선발하였다. 이들 중에서 primer 제작이 잘된 beta-1, Orla C3-1, parvalbumin 및 apolipoprotein E을 선발하여 그 유전자 발현을 real-time PCR 기법을 사용하여 정량적으로 모니터링 하였다. Orla C3-1, parvalbumin 및 apolipoprotein E는 고농도의 다이아지논 처리(1000 ppb; 24 h)에서 그 발현이 억제됨이 관찰되었다. 다이아지논 처리 시 신경질환 (알츠하이머 병 및 다운신드롬)에 관련된 apolipoprotein E와 근육세포의 유연화에 작용하는 parvalbumin 등의 발현억제는 송사리의 인지능력 교란 및 근육세포의 경직 등을 각각 유도하여 송사리의 비정상적 행동을 야기하는 것으로 판단되었다. 따라서 이들 생물지표는 신경독성물질에 의한 송사리 및 기타 어류의 이상행동의 변화의 감지에 활용될 수 있을 것으로 사료된다.

Changes in the Fruit Quality of Organic and Low-level Agrochemical-grown Kiwifruit during Storage (유기농 및 저농약 재배한 참다래의 저장 중 품질변화)

  • Park, Yong-Seo;Lim, Dong-Guen;Heo, Buk-Gu
    • Food Science and Preservation
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    • v.16 no.3
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    • pp.327-332
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    • 2009
  • Kiwifruits conventionally grown (CG), grown with low levels of chemicals (LCG), and organically grown (OG), were kept in cold storage for 24 weeks. Firmness gradually decreased with increasing storage time, regardless of cultivation mode, and the rate of softening was slightly higher in OG than in CG or LCG fruit. Neither dry matter level nor sensory values differed with varying types of cultivation. Soluble solid content increased with storage time, whereas acidity decreased in all fruit. Reducing sugar content increased notably until 12 weeks of storage, whereas starch content significantly decreased. The rate of OG fruit decay abruptly increased mid-storage and reached 35% 24 weeks after storage. Most fruit decayed due to infection with Botritis cinerea, regardless of cultivation type. Respiration and ethylene content peaked at mid-storage and were both slightly higher in OG fruit than in CG or LCG fruit. The shelf life of kiwifruit was reduced in OG fruit by increased fruit decay and softening during storage.