One-day-old Cherry valley meat-strain ducks were used to investigate the effect of supplemental dried oregano powder (DOP) in feed on the productivity, antioxidant enzyme activity, and breast meat quality. One hundred sixty five ducks were assigned to 5 dietary treatments for 42 days. The dietary treatment groups were control group (CON; no antibiotic, no DOP), antibiotic group (ANT; CON+0.1% Patrol), 0.1% DOP (CON+0.1% DOP), 0.5% DOP (CON+0.5% DOP), and 1.0% DOP (CON+1.0% DOP). Upon feeding, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity of oregano extracts was higher than that of tocopherol, although it was less than that of ascorbic acid. As a result of in vivo study, DOP in the diet showed no effects on final body weight, feed intake, or feed conversion ratio. However, dietary 0.5% and 1% DOP supplementation caused a significant increase in the serum enzyme activity of superoxide dismutase (SOD) compared with CON and ANT, while glutathione peroxidase (GPx) in tissue was increased as compared to ANT (p<0.05). Cooking loss from ducks fed with DOP decreased compared with the control ducks. Thiobarbituric acid reactive substance (TBARS) values of duck breast meat at 5 d post slaughter was found to be significantly reduced in ducks whose diets were supplemented with 0.5% and 1% DOP (p<0.05). These results suggest that diets containing 0.5% and 1% DOP may beneficially affect antioxidant enzyme activity of GPx and SOD, improve meat cooking loss, and reduce TBARS values in breast meat at 5 d of storage in ducks.
Lee, Sang Hoon;Jang, Gwi Yeong;Hwang, In Guk;Kim, Hyun Young;Woo, Koan Sik;Kim, Kee Jong;Lee, Mi Ja;Kim, Tae Jip;Lee, Junsoo;Jeong, Heon Sang
Preventive Nutrition and Food Science
/
v.20
no.2
/
pp.110-118
/
2015
This study was performed to investigate changes in the content and purity, as well as physical characteristics of ${\beta}$-glucan extracted from acid hydrolyzed whole grain barleys. Waxy and non-waxy barleys (Hordeum vulgare) were hydrolyzed with different concentrations of HCl (0.1~0.5 N) for 1 h. As the HCl concentration increased, the contents of total and soluble ${\beta}$-glucan from acid hydrolyzed barley decreased. However the ratio of soluble/total ${\beta}$-glucan content and purities of ${\beta}$-glucan significantly increased. The ratio of ${\beta}-(1{\rightarrow}4)/{\beta}-(1{\rightarrow}3)$ linkages, molecular weight, and viscosity of soluble ${\beta}$-glucan of raw barleys were 2.28~2.52, $6.0{\sim}7.0{\times}10^5g/mol$, and 12.8~32.8 centipoise (cP). Those of isolated soluble ${\beta}$-glucan were significantly decreased to 2.05~2.15, $6.6{\sim}7.8{\times}10^3g/mol$, and 3.6~4.2 cP, respectively, with increasing acid concentration. The re-solubility of raw barley ${\beta}$-glucan was about 50%, but increased to 97% with increasing acid concentration. Acid hydrolysis was shown to be an effective method to produce ${\beta}$-glucan with high ratio of soluble ${\beta}$-glucan content, purity, water solubility, and low viscosity.
The effects of additions of lotus leaf (0.1 and 0.5%) and barley leaf powder (0.1 and 0.5%) on the lipid oxidation and microbiological analysis of raw minced pork were investigated after 1, 4, 7, and 10 d at chilled storage. Days of storage caused (p<0.05) decreases in pH values in samples with lotus leaf (LP) and barley leaf powder (BP). $L^*$ and $a^*$ values decreased, and $b^*$ values increased in the treatments with increasing lotus leaf and barley leaf powder contents, respectively. The decrease in $a^*$ values was lowest (p<0.05) in the treatment with 0.1% BP. Thiobarbituric acid reaction substance values and free fatty acids in 0.5% LP were lowest (p<0.05) on day 10. Thus, the addition of lotus leaf powder significantly improved lipid oxidative stability in the raw minced pork during chilled storage of 10 d. Furthermore, the raw minced pork treatments with LP and BP presented low peroxide values and total microbes as compared to control (-) (without LP and BP). These results indicate that LP and BP can be incorporated into raw minced pork as natural additives to retard oxidation.
Kim, Yu-Kyoung;Kang, Ho-Jun;Yang, Sang-Ho;Oh, Han-Jun;Lee, Shin-Chan;Kang, Seong-Keun;Kim, Hyoung-Sin
Korean Journal of Organic Agriculture
/
v.20
no.4
/
pp.687-702
/
2012
The objective of this study was to determine crop rotation effects on potato yield, soil chemical and microbiological properties from a short-term field experiment from 2010 to 2011 in Jeju Island, Korea. Potato cropping systems included continuous and rotation sequences of soybean (Glycine max(L.) Merr.), barley (Hordeum vulgare var. hexastichon), rapeseed (Brassica napus L.) and broccoli (Brassica oleracea var. italica). Crop rotations increased the yields of potato from 31% to 52% compared with continuous potato. Marketable yield of potato was highest under soybean plus rapeseed rotation by $20.97MT\;ha^{-1}$ and lowest under continuous cropping by $11.95MT\;ha^{-1}$. The incidence and severity of scab disease was significantly lower in tubers from crop rotation with soybean plus barley. Differences in marketable tuber yields among rotations were associated with potato scab disease. Especially, incidence and severity of potato scab were strongly correlated with soil pH, exchangeable calcium, and bacteria population of the soil. Crop rotations significantly increased soil pH, available phosphate, exchangeable K and Ca, especially in crop rotations with soybean plus barley or rapeseed. Soil microbial biomass C of crop rotations with soybean plus barley or rapeseed, was also significantly higher compared with monoculture. In conclusion, crop rotation may decrease the incidence of soil-born pathogen by increasing soil chemical properties and soil microbial biomass. Overall, potato crop productivity was generally maintained in rotations that contained soybean plus barley or rapeseed but declined under continuous cropping system.
Grazing rate (GR) and feces production (FP) of freshwater snail Cipangopaludina chinensis malleata on two hibernal diatom communities were examined in a laboratory. Snail with the similar size (4.2$\sim$5.8 cm) were collected from the Gunsan and Okgu district (Jeonbuk), transferred to the artificial management system in laboratory, and starved for 3 day before the experiment. The feeding experiments were conducted at various conditions such as passage of time (0, 1, 4 and 7 hr), snail density (0.0, 0.5, 1.0, 2.0 and 4.0 ind. $L^{-1}$) and kinds of prey (cold lake and stream water). One prey used in this study is the water of Lake Ilgam, the other is that of Han river. Lake Ilgam water was dominated by Synedra ulna (69.1%) and Scenedesmus sp. (6.6%), while Han river was by Asterionella formosa (69.4%) and Diatoma vulgare (27.7%). With the increment of snail density and time, the Chl-a concentration of two experimental waters were clearly decreased. Chl-a of Han river rapidly was decreased after 1 hour of snail treatment, while that of Ilgam lake was decreased after 4 hour. On the passage of time, a highest GR (1.94 L $g^{-1}\;h^{-1}$) showed at 1 hr, and then, decreased gradually to 0.04 L $g^{-1}\;h^{-1}$ by 7 hr. The highest FP (0.11 mg $L^{-1}$) showed at 7 hr. These results indicate that native snail Cipangopaludina chinensis malleata may be applied as a potential bio-filter to control diatom blooms in the cold lake and stream.
In order to evaluate the physicochemical properties of sludge compost and to identify the effects water-extracts from sludge compost in 2, 6, 8, 12 weeks of decomposing process on seed germination and root elongation in cabbage, lettuce, soybean and barley plants was investigated. The content of total nitrogen in sludge compost increased slightly in 6 weeks decomposing process, and then decreased gradually. Organic matter content decreased continuously overall decomposing process. As decomposition was processing, pH of sludge compost decreased slightly, and EC increase within 6 weeks decomposing process, and then decreased. The content of nitrogen in water-extracts from sludge compost increased within 8 weeks decomposing process and decreased in 12 weeks decomposing process. The content of ammonium nitrogen was similar with that of total nitrogen, and the ratio of ammonium and nitrate increased within 8 weeks, and then decreased. Cation content and EC decreased the late of decomposing process and pH didn't change. The water-extracts from sludge compost during decomposing process inhibited seed germination and root elongation in cabbage (Brassica campestris), lettuce (Lactuca sativa), barley (Hordeum vulgare) and soybean (Glycine max). The inhibition of root elongation in cabbage was greater than that of relative seed germination, whereas relative seed germination in lettuce was more inhibit than root elongation. Relative seed germination and root elongation in soybean were inhibited slightly, but those of in barley was inhibited strongly. In this study, we would identify the effects of water-extracts from sludge compost on seed germination and root elongation was different to the species of seed. The inhibition of seed germination and root elongation treated with the water-extracts which extracted from sludge compost in the early stage of decomposing process was greater than that of in the late stage of decomposing process.
In this study, we investigated the potential of Lactobacillus brevis AR1 fermented broth containing various grains (Fagopyrum esculentum, Scotch oat, Sesamum indicum, Glycine max Merr, Castanea crenata, Oryza sativa L., Hordeum vulgare L., Perilla frutescens var. japonica Hara, or Triticum aestivum L.) or Saccharina japonica as a source of collagen synthesis in cosmetic products. The treatment of Lb. brevis AR1 fermented broths containing F. esculentum or S. japonica water extracts was markedly increased the synthesis of collagen in fibroblasts. The collagen synthesis capacity of the S. japonica fermentation product was higher than that of β-glucan, which was used as a positive control. Under controlled conditions in broths containing F. esculentum or the S. japonica extracts with 4% monosodium glutamate (MSG), Lb. brevis AR1 produced γ-aminobutyric acid (GABA) at a concentration of 180 mM, with an 84.5% GABA conversion rate after 72 h. Both the F. esculentum and S. japonica fermentation broths produced by Lb. brevis AR1 reduced inflammatory responses on mouse skin and did not show cell cytotoxicity in fibroblasts. These results suggest that both the F. esculentum and S. japonica fermentation products of Lb. brevis AR1 could be used as functional materials in cosmetic products to combat wrinkles and skin inflammation.
Kim, S.Y.;De Datta, S.K.;Robles, R.P.;Kim, K.U.;Lee, S.C.;Shin, D.H.
Korean Journal of Weed Science
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v.14
no.1
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pp.34-41
/
1994
To better understand the allelopathic effect of sorghum(Sorghum vulgare L.), the inhibitory activities of water extracts of the stem, leaf and root, and of residues of the stem to major crops and weeds associated with them were evaluated. The allelopathic activity of sorghum plants was species specific, and depended on source and concentration. Germination, and shoot and root length of all test species were inhibited by the different concentrations of the stem extract. Among the crop species, radish showed the most inhibition, followed by wheat and rice. Maize was the least sensitive species. Of the weed species, Ipomoea triloba was most inhibited, followed by Echinochloa colona and Rottboellia cochinchinensis. The water extracts of leaves, stems, and roots significantly inhibited germination and seedling growth in E. colona and radish. The stem extract gave the greatest inhibitory effect on E. colona while all three extracts produced similar response in radish. In the greenhouse trial, sorghum stem residue placed on the soil surface as mulch significantly inhibited seedling growth in E. colona and radish, but not that in rice.
Lombardi, Valter RM;Eetcheverria, Ignacio;Fernandez-Novoa, Lucia;Diaz, Joaquin;Seoane, Silvia;Cacabelos, Ramon
Advances in Traditional Medicine
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v.5
no.3
/
pp.216-230
/
2005
Although the field of study in immune enhancing compounds is relatively new, natural products from plants represent a rich and promising source of novel molecules with immunomodulating properties, Microglial cells, the main immune effector cells of the brain, usually display a ramified morphology and low expression levels of immunologically relevant antigens such as MHC class I and class II. Since any compound which participates in activation of phagocytic cells contributes to the production of potentially toxic factors, the search for convenient in vitro test-systems and study of mechanisms of action of these agents are of great interest. Human blood polymorphonuclear (PMN) cells and primary microglial cells isolated from Sprague-Dawley rats were used as cellular screening tests for study of phagocytosis-stimulating action of immunomodulating agents. Numbers of phagocytic activity were evaluated by the phagocyte ingestion of yeast cells and NO-synthase activity, nitrite production, and nitroblue tetrazolium test were determined after phagocyte stimulation. It was possible to demonstrate that indexes of phagocytic activity can be used as quantitative indicators for measurement immunomodulating activity. As a positive control, Zymosan A-induced phagocytosis in both PMN cells and primary microglial cells was used. $IFN-{\gamma}$ (0.1 -1 U/ml) stimulated phagocytosis in PMN cells 1.2 times after 2 - 3 h incubation, although at higher concentrations (10 - 100 U/ml) it strongly inhibited phagocytosis. In a similar way, at higher concentrations, $IFN-{\gamma}$ (100 - 500 U/ml) suppressed phagocytosis in zymosan-A stimulated microglial cells. When Polypodium leucotomus, cambricum and vulgare extracts were tested alone, increased levels of phagocytosis were observed in PMN. In addition, microglial cells showed both increased phagocytosis and MHC class-II antigen expressions. Surprisingly, when PMN and microglia were treated with a combination of Polypodium and $IFN-{\gamma}$, phagocytosis was not inhibited. We did not find changes in NO-synthase activity and nitrite production in both microglia and PMN cells activated by different immunomodulating agents. These results indicate that primary microglial cell cultures as well as human PMN cells can provide reproducible quantitative results in screening phagocytic activity of different immunoactive compounds. Furthermore, both inhibitory or activation mechanisms might be studied using these in vitro experimental approaches.
Journal of The Korean Society of Grassland and Forage Science
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v.29
no.4
/
pp.345-354
/
2009
The barley (Hordeum vulgare L.) is now widely grown as a whole crop silage in Korea, but the quality of that silage does not examined from farms. Therefore, this experiment was conducted to evaluate the forage quality of whole crop barley that was participated in Silage Quality Contest in 2008. These data were classified by region, dry matter (DM) yield, with or not additive, planting and harvest date. Difference on the lactic acid content of barley silage was detected in the region, DM yield, additive and harvest date (p<0.05), however, there were partially significant differences in chemical composition. There were significant differences among moisture content in DM yield, pH in additive, and crude ash content in additive and planting date of barley silage. Crude protein content was significant difference in the region, and ether extract content was in region, DM yield and harvest date, however, non-fiber carbohydrate was significant difference in planting date of barley silage. Neutral detergent fiber, acid detergent fiber and relative feed value were significant difference only in the cultivation region of whole crop barley. According to this experiment, differences in the silage quality were observed among whole crop barley silages. Therefore, nutritive value as well as moisture and pH are important in silage quality evaluation of whole crop barley. Especially, lactic acid is an important factor for the evaluation of whole crop barley silage.
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