• Animal Systematics, Evolution and Diversity
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• v.9 no.2
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• pp.251-259
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• 1993

The soil hypotrichs collected from the moss-covered soils in the campus of Seoul National University were identified as Holosticha multistylata Kahl, 1928 and H. sylvatica Foissner, 1982. These two species are discovered for the first time from Korea and redescribed with illustrations. The description was based on the observation of living specimens, protargol impregnated specimens and biometric analysis. They are distinguished from congeneric species by their following characteristics : 4 frontal cirri in H. multistylata and 7-8 in H. sylvatica, 3 buccal cirri in H. multistylata and 1 in H. sylvatica, 3 dorsal kineties in H. multistylata and 5 in H. sylvatica.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.65-71
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• 2003

This research aims the production of anti-tumor substances through in vitro culture of hairy roots transformed by Agrobacterium rhizogenes in Artemisia sylvatica MAX and the effect of culture conditions on optimum growth of hairy roots. We investigated the optimum medium, pH, carbon source, sucrose, light, Fe and polyamine conditions of various lines of hairy roots (NK3, NK4, YX. NK3-10) induced from Artemisia sylvatica to increase the optimum growth of hairy roots. MS medium was the best for optimum growth of hairy root clone, NK3-S10. The optimum culture period was 4 weeks for NK3-S10. The optimum sucrose concentration was 3.5%. The optimum concentration of FeSO$_4$, spermine and spermidine was 0.1 mM, 10 mM and 100 mM, respectively.

• Korean Journal of Environment and Ecology
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• v.17 no.2
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• pp.144-152
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• 2003

The purpose of this study was to investigate the effects of lime fertilization and light intensity on beech regeneration strategy in a mature beech forest(ca. 150 years old) gaps, which was located in the soiling areas Germany, where the soil is an acid brown earth. For the experiment canopy gaps of 30m diameter were cut, and one canopy gap remained as untreated while the other was limed(3t dolomite h $a^{-1}$). Soil chemistry, soil water potential, light intensity(PAR), ground vegetation, beech seedlings and mycorrhiza were investigated along transect from the stands into gaps to the north. The results of this study are followings: 1) In the limed gap, soil chemistry was greatly improved and a vigorous herb layer developed as compared to the unlimed gap. 2) Development and growth of the beech seedlings in the unlimed gap were generally deficient. Specially, developments of lateral roots were very lack. 3) Through the lime fertilization, the minerals contents of beech leaves such as calcium(Ca) and magnesium(Mg) were increased, while manganese(Mn) was decreased. There was antagonism between potassium(K) and calcium(Ca) or magnesium(Mg). 4) The relative mycorrhiza frequency(RM) in beech seedlings of the southern edge was very higher than that of the middle part, and through the lime fertilization extension of the species Laccaria amethystina appeared clearly. The results of this study showed that lime fertilize in forest, where the soil is acid, will be necessary..

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.67.1-67.1
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• 2003

Three new guaianolide type of sesquiterpene lactones, 8${\alpha}$-angeloyloxy-1${\alpha}$-hydroxy-3${\alpha}$,4${\alpha}$-epoxy-5${\alpha}$, 7${\alpha}$H-10(14), 11(13)-guaiadien-12,6${\alpha}$-olide (1), 8${\alpha}$-methylbutyryloxy-1${\alpha}$-hydroxy-3${\alpha}$, 4${\alpha}$-epoxy-5${\alpha}$, 7${\alpha}$H-10(14),11(13)-guaiadien-12,6${\alpha}$-olide (2), and 8${\alpha}$-isovaleryloxy-1${\alpha}$-hydroxy-3${\alpha}$, 4${\alpha}$-epoxy-5${\alpha}$, 7${\alpha}$H-10(14),11 (13)- guaiadien-12,6${\alpha}$-olide (3), together with six known sesquiterpenes, artemisolide (4), 3-methoxytanapartholide (5), deacetyllaurenobiolide (6), moxartenolide (7), arteminolide B (8), and arteminolide D (9) were isolated by bioassay-guided fractionation using the NF-kB mediated reporter gene assay system. (omitted)

• Journal of the Korean Wood Science and Technology
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• v.34 no.6
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• pp.36-43
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• 2006

Using fluidized bed type fast pyrolysis system (capacity 400 g/h) bio-oils were produced from beech (Fagus sylvatica) and softwood mixture (spruce and larch, 50:50). The pyrolysis was performed for 1~2 s at the temperature of $470{\pm}5^{\circ}C$. Pyrolysis products consisted of liquid form of bio-oil, char and gases. In beech wood bio-oil was formed to ca. 60% based on dry biomass weight and the yield of bio-oil was 49% in soft wood mixture. The moisture contents in both bio-oils were ranged between 17% and 22% and the bio-oil's density was measured to $1.2kg/{\ell}$. Bio-oils were composed of 45% carbon, 47% oxygen, 7% hydrogen and lower than 1% nitrogen,which was very similar to those of original biomass. In comparison with oils from fossil resources, oxygen content was very high in bio-oils, while no sulfur was found. More than 90 low molecular weight components, classified to aromatic and non aromatic compounds, were identified in bio-oils by gas chromatographic analysis, which amounted to 31~33% based on the dry weight of bio-oils.