• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.783-790
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• 2007

The immobilization of proteins and their molecular interactions on various polymer-modified glass substrates [i.e. 3-aminopropyltriethoxysilane (APTS), 3-glycidoxypropyltrimethoxysilane (GPTS), poly (ethylene glycol) diacrylate (PEG-DA), chitosan (CHI), glutaraldehyde (GA), 3-(trichlorosilyl)propyl methacrylate (TPM), 3'-mercaptopropyltrimethoxysilane (MPTMS), glycidyl methacrylate (GMA) and poly-l-lysine (PL).] for potential applications in a nanoarray protein chip at the single-molecule level was evaluated using prismtype dual-color total internal reflection fluorescence microscopy (dual-color TIRFM). A dual-color TIRF microscope, which contained two individual laser beams and a single high-sensitivity camera, was used for the rapid and simultaneous dual-color detection of the interactions and colocalization of different proteins labeled with different fluorescent dyes such as Alexa Fluor® 488, Qdot® 525 and Alexa Fluor® 633. Most of the polymer-modified glass substrates showed good stability and a relative high signal-to-noise (S/N) ratio over a 40-day period after making the substrates. The GPTS/CHI/GA-modified glass substrate showed a 13.5-56.3% higher relative S/N ratio than the other substrates. 1% Top-Block in 10 mM phosphate buffered saline (pH 7.4) showed a 99.2% increase in the blocking effect of non-specific adsorption. These results show that dual-color TIRFM is a powerful methodology for detecting proteins at the single-molecule level with potential applications in nanoarray chips or nano-biosensors.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.30 no.6
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• pp.559-566
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• 2017

This paper proposes a new composite deckplate system reinforced with inverted triangular truss girders(called 'D Deck'), which does not require the use of temporary supports at construction stage. The proposed system retains increased stiffness and strength while keeping the absolute floor height change to a minimum level and can be utilized as floor systems of various types beam members such as the conventional wide-flange and U-shaped composite beams. In order to evaluate the performance of the proposed system, five specimens with a span of 5.5 m were fabricated and tested under field loading conditions consisting of several intermediate steps. The load-deflection curves of each specimen were plotted and compared with the nonlinear three-dimensional finite element analysis results. The comparison showed that the effective load sharing between the truss girders and floor deck occurs and the maximum deflection under construction stage loading is well below the limit estimated by the provisions in Korea Building Code.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.7 no.2
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• pp.59-68
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• 1987

Several series of fracture tests were conducted to explore the fracture characteristics and to determine the fracture energy of concrete. A stable three-point bend test was employed to generate the load-deflection curves. The fracture energy may then be calculated from the area under the complete load-deflection curve. The initial notch-to-beam depth ratio (${\alpha}_0$/H) was varied from zero to 0.6. The prediction formula for the fracture energy of concrete is also derived and is found to depend on the tensile strength and aggregate size. The proposed fracture energy formula can be used for the fracture analysis of concrete structures. The present study also devises an equivalent crack length concept to predict the maximum failure loads of concrete beams. A simple formula for the equivalent crack length is proposed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.4
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• pp.293-300
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• 2012

In this study, the delamination analysis has been implemented to investigate the initiation and propagation of crack in composite laminates composed of orthotropic materials. A simple modeling was achieved by moving nodal technique without re-meshing work when crack propagation occurred. This paper aims at achieving two specific objectives. The first is to suggest a very simple modeling scheme compared with those applied to conventional h-FEM based models. To verify the performance of the proposed model, analysis of double cantilever beams with composite materials was implemented and then the results were compared with reference values in literatures. The second one is to investigate the behavior of interior delamination problems using the proposed model. To complete these objectives, the full-discrete-layer model based on Lobatto shape functions was considered and energy release rates were calculated using three-dimensional VCCT(virtual crack closure technique) based on linear elastic fracture mechanics.

• Steel and Composite Structures
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• v.22 no.6
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• pp.1417-1443
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• 2016

Cellular and castellated steel beams are used to obtain higher stiffness and bending capacity using the same weight of steel. In addition, the beam openings may be used as a pass for different mechanical fixtures such as ducts and pipes. The aim of this study is to investigate the effect of different parameters on both elastic and inelastic critical buckling stresses of steel web plates with openings. These parameters are plate aspect ratio; opening shape (circular or rectangular); end distance to the first opening; opening spacing; opening size; plate slenderness ratio; steel grade; and initial web imperfection. The web/flange interaction has been simplified by web edge restraints representing simply supported boundary conditions. A numerical parametric study has been performed through linear and nonlinear finite element (FE) models, where the FE results have been verified against both experimental and numerical results in the literature. The web plates are subject to in-plane linearly varying compression with different loading patterns, ranging from uniform compression to pure bending. A buckling stress modification factor (${\beta}$-factor) has been introduced as a ratio of buckling stress of web plate with openings to buckling stress of the corresponding solid web plate. The variation of ${\beta}$-factor against the aforementioned parameters has been reported. Furthermore, the critical plate slenderness ratio separating elastic buckling and yielding has been identified and discussed for two steel grades of DIN-17100, namely: ST-37/2 and ST-52/3. The FE results revealed that the minimum ${\beta}$-factor is 0.9 for web plates under uniform compression and 0.7 for those under both compression and tension.

• Journal of Korean Society of Steel Construction
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• v.19 no.5
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• pp.483-492
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• 2007

Pre-Engineering Building (PEB) system is one of the most economical structural systems. Tapered members can resist a maximum stress at a single location, whereas stresses of the rest of the members are considerably low. This results in appreciable savings both in terms of materials and construction costs. However, it was appreciated that special consideration would be required for certain aspects of this structural form. In particular, because of their slenderness, webs would buckle laterally and torsionally under the combined action of excessive axial, bending and shear forces. In this study, a total of four large-scale rafters with simple ends were tested. The main parameters were the width-thickness ratio of the web, the stiffener, and the flange brace. The purpose of this experiment is to evaluate the structural stability and to offer back-data on PEB design.

• Journal of the Korean Society for Nondestructive Testing
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• v.20 no.6
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• pp.481-489
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• 2000

A time-frequency analysis method was developed to analyze the dispersive waves caused by impact loads in structural members such as beams and plates. Stress waves generated by ball drop and pencil lead break were recorded by ultrasonic transducers and acoustic emission (AE) sensors. Wavelet transform (WT) using Gabor function was employed to analyze the dispersive waves in the time-frequency domain, and then to find the arrival time of the waves as a function of frequency. The measured group velocities in the beam and the plate were compared with the predictions based on the Timoshenko beam theory and Rayleigh-Lamb frequency equations, respectively. The agreements were found to be very good.

• Earthquakes and Structures
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• v.18 no.6
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• pp.677-689
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• 2020

A building structural system of moment resisting frame (MRF) with concrete filled steel tubular (CFST) columns and wide flange H beams, is one of the most conveniently constructed structural systems. However, there were few studies on evaluating seismic performance of full-scale CFST columns under high axial compression. In addition, some existing famous design codes propose various limits of width-to-thickness ratio (B/t) for steel tubes of the ductile CFST composite members. This study was intended to investigate the seismic behavior of CFST columns under high axial load compression. Four full-scale square CFST column specimens with a B/t of 42 were carried out that were subjected to horizontal cyclic-reversal loads combined with constantly light, medium and high axial loads and with a linearly varied axial load, respectively. Test results revealed that shear strength and deformation capacity of the columns significantly decreased when the axial compression exceeded 0.35 times the nominal compression strength of a CFST column, P₀. It was obvious that the higher the axial compression, the lower both the shear strength and deformation capacities were, and the earlier and faster the shear strength degradation occurred. It was found as well that higher axial compressions resulted in larger initial lateral stiffness and faster degradation of post-yield lateral stiffness. Meanwhile, the lower axial compressions led to better energy dissipation capacities with larger cumulative energy. Moreover, the study implied that under axial compressions greater than 0.35P₀, the CFST column specimens with B/t limits recommended by AISC 360 (2016), ACI 318 (2014), AIJ (2008) and EC4 (2004) codes do not provide ultimate interstory drift ratio of more than 3% radian, and only the limit in ACI 318 (2014) code satisfies this requirement when axial compression does not exceed 0.35P₀.

• Smart Structures and Systems
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• v.24 no.5
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• pp.631-639
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• 2019

Finite element analysis is one of the important methods to study the structural performance. Due to the simplification, discretization and error of structural parameters, numerical model errors always exist. Besides, structural characteristics may also change because of material aging, structural damage, etc., making the initial finite element model cannot simulate the operational response of the structure accurately. Based on Bayesian methods, the initial model can be updated to obtain a more accurate numerical model. This paper presents the work on the field test, modal identification and model updating of a Chinese reinforced concrete pagoda. Based on the ambient vibration test, the acceleration response of the structure under operational environment was collected. The first six translational modes of the structure were identified by the enhanced frequency domain decomposition method. The initial finite element model of the pagoda was established, and the elastic modulus of columns, beams and slabs were selected as model parameters to be updated. Assuming the error between the measured mode and the calculated one follows a Gaussian distribution, the posterior probability density function (PDF) of the parameter to be updated is obtained and the uncertainty is quantitatively evaluated based on the Bayesian statistical theory and the Metropolis-Hastings algorithm, and then the optimal values of model parameters can be obtained. The results show that the difference between the calculated frequency of the finite element model and the measured one is reduced, and the modal correlation of the mode shape is improved. The updated numerical model can be used to evaluate the safety of the structure as a benchmark model for structural health monitoring (SHM).

• Steel and Composite Structures
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• v.46 no.6
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• pp.773-791
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• 2023

Connections with damage concentrated to pre-selected components can enhance seismic resilience for moment resisting frames. These pre-selected components always yield early to dissipate energy, and their energy dissipation mechanisms vary from one to another, depending on their position in the connection, geometry configuration details, and mechanical characteristics. This paper presents behaviour insights on two types of beam-to-beam connections that the angles were designed as energy dissipation components, through the results of experimental study and finite element analysis. Firstly, an experimental programme was reviewed, and key responses concerning the working mechanism of the connections were presented, including strain distribution at the critical section, section force responses of essential components, and initial stiffness of test specimens. Subsequently, finite element models of three specimens were established to further interpret their behaviour and response that were not observable in the tests. The moment and shear force transfer paths of the composite connections were clarified through the test results and finite element analysis. It was observed that the bending moment is mainly resisted by axial forces from the components, and the dominant axial force is from the bottom angles; the shear force at the critical section is primarily taken by the slab and the components near the top flange. Lastly, based on the insights on the load transfer path of the composite connections, preliminary design recommendations are proposed. In particular, a resistance requirement, quantified by a moment capacity ratio, was placed on the connections. Design models and equations were also developed for predicting the yield moment resistance and the shear resistance of the connections. A flexible beam model was proposed to quantify the shear resistance of essential components.