• Transactions of the Korean hydrogen and new energy society
• /
• v.17 no.4
• /
• pp.448-453
• /
• 2006

Porous Co-P catalysts electroplated on Cu in chloride based solution with an addition of $NaH_2PO_2$ and glycine were developed for hydrogen generation from alkaline $NaBH_4$ solution. The microstructures of the Co-P catalysts and their hydrogen generation properties were analyzed as a function of cathodic current density and plating time during the electrodeposition. Amorphous Co-P electrodeposits with porous structure was formed on Cu at cathodic current density of $0.05\;A/cm^2$, and showed very high hydrogen generation rate in alkaline $NaBH_4$ solution due to an increase in the surface area of the catalyst as well as the catalytic activity. The Co-P catalyst, which was obtained at cathodic current density of $0.05\;A/cm^2$ for 5 min, exhibited the best hydrogen generation rate of 2290 ml/min.g-catalyst in 1 wt. % NaOH+10 wt. % $NaBH_4$ solution at $30^{\circ}C$.

• Microbiology and Biotechnology Letters
• /
• v.34 no.1
• /
• pp.40-46
• /
• 2006

A bacterial strain producing high level of a phytase was isolated from cattle feces and identified as Bacillus subtilis, and designated as Bacillus sp. CF 5-26. The production of the phytase from Bacillus sp. CF 5-26 reached the highest level after 72 hours at $37^{\circ}C$. The optimum condition of the media for the production of phytase was 10% rice bran extract, 0.1% whey protein powder, $0.01%\;CaCl_{2},\;0.01%\;KH_{2}PO_4$. The phytase was purified 20.3 folds with ethanol precipitation, Sephadex G-100, CM Sepharose CL-6B and Sephacryl S-100-HR column chromatography. The molecular weight of the purified enzyme was estimated to be 66 kDa on SDS-polyacrylamide gel electrophoresis. The purified phytase activity was stable up pH 5.0, 7.0, 11.0 and the remaining activity was 50% when it was treated at $100^{\circ}C$ for 1 hour. The substrate specificity of phytase was most active against sodium phytate and inositol polyphosphate compound. And the phytase hydrolysed tripolyphosphate and pyrophosphate a little. The Km value for the sodium phytate was 0.64 mM and the Vmax value was $4.41\;{\mu}mol/min$.

• Korean Journal of Pharmacognosy
• /
• v.37 no.2 s.145
• /
• pp.67-73
• /
• 2006

The butanol extracts of Agrimonia pilosa (BAP) induced dose-dependent vascular relaxation of phenylephrine-precontracted aorta, which was abolished by removal of functional endothelium. Pretreatment of the endothelium-intact aortic tissues with $N^G$-nitro-L-arginine methyl ester (L-NAME) and 1H-[1,2,4]-oxadiazole-[$4,3-{\alpha}$]-quinoxalin-1-one(ODQ) inhibited the relaxation induced by BAP. BAP-induced vascular relaxation was also markedly attenuated by addition of verapamiI, while the relaxant effect of BAP was not blocked by indomethacine, glibenclamide, tetraethylammonium (TEA), atropine, or propranolo. In addition, incubation of endothelium-intact aortic rings with BAP increased the vascular production of cGMP. These results suggest that BAP relaxes vascular smooth muscle via endothelium-dependent nitric oxide/cGMP signaling pathway, which may be causally related with L-type $Ca^{2+}$ channels.

• Journal of the Korean Applied Science and Technology
• /
• v.28 no.4
• /
• pp.508-516
• /
• 2011

Hydrotreated biodiesel(HBD) is paraffinic bio-based liquid, with the chemical structure $C_nH_{2n+2}$, originating from vegetable oil(the process can also be applied to animal fat). The oil or fat is treated in a number of process, the most important being hydrogenation, in order to create a bio-based liquid diesel fuel. During the hydrogenation, oxygen is removed from the triglyceride and converted into water. Propane is formed as a by product and can be combusted and used for energy production. HBD can be used in conventional diesel engines, pure or blended with conventional diesel, due to its similar physical properties to diesel. This study reports the quality characteristics with chemical and physical properties as an alternative diesel fuel. Especially, HBD showed higher cetane value and number than FAME, and it is consisted of $C_{15}$ - $C_{18}$ n-paraffinic compounds. We also describes quality characteristics of HBD blends(2, 5, 10, 20, 30, 40, 50 vol%) in automotive diesel. HBD blends(max. 20 vol%) were the limit by the Korean specification due to poor low temperature characteristics.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2005.06a
• /
• pp.1206-1209
• /
• 2005

In recent, injection molding process for features in sub-micron scale is under active development as patterning nano-scale features, which can provide the master or stamp for molding, and becomes available around the world. Injection molding has been one of the most efficient processes for mass production of the plastic product, and this process is already applied to nano-technology products successfully such as optical storage media like DVD or BD which is a large area plastic thin substrate with nano-scale features on its surface. Bio chip for like DNA sequencing may be another application of this plastic substrate. The DNA can be sequenced using order of 100 nm pore structure when making the DNA flow through the pore structure. Agarose gel and silicon based chip have been used to sequence the DNA, but injection molded plastic chip may have benefit in terms of cost. This plastic DNA sequencing chip has plenty of pillars in order of 100 nm in diameter on the substrate. When the usual features in case of DVD or BD have very low aspect ratio, even less than 0.5, but the DNA chip will have relatively high aspect ratio of about 2. It is not easy to injection mold the large area thin substrate with sub-micron features on its surface due to the characteristics of the molding process and it becomes much more difficult when the aspect ratio of the features becomes high. We investigated the effect of the molding parameters for injection molding with high aspect ratio nano-scale features and injection molded some plastic DNA sequencing chips. We also fabricated PR masters and Ni stamps of the DNA chip to be used for molding

• Food Science of Animal Resources
• /
• v.35 no.2
• /
• pp.265-271
• /
• 2015

In a previous study, hydrolysates of porcine placenta were obtained and the extraction efficiency for proteins and amino acids was compared between sub- and super-critical water extraction systems; optimum efficiency was found to be achieved using subcritical water ($170^{\circ}C$, 10 bar). In this study, the effects of adding ethanol to the subcritical water system were investigated. The lowest-molecular-weight extraction product detected weighed 434 Da, and the efficiency of extraction for low-molecular-weight products was increased when either the concentration of ethanol was decreased, or the extraction time was lengthened from 10 min to 30 min. The highest concentration of free amino acids (approximately 8 mM) was observed following 30 min extraction using pure distilled water. The concentration of free amino acids was significantly lower when ethanol was added or a shorter extraction time was used (p<0.05). Color change of the solution following extraction was measured. There were no significant differences in color between lysates produced with different extraction times when using distilled water (p>0.05); however, using different extraction times produced significant differences in color when using 20% or 50% ethanol solution for subcritical extraction (p<0.05). The range of pH for the hydrolysate solutions was 6.4-7.5. In conclusion, the investigated extraction system was successful in the extraction of $\leq$ 500 Da hydrolysates from porcine placenta, but addition of ethanol did not yield higher production of low-molecular-weight hydrolysates than that achieved by DW alone.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.5
• /
• pp.630-635
• /
• 2000

Previous research has not evaluated the effects of various rates and frequencies of manure application and frequencies of cutting on yield and stand persistence of cool-season grasses and alfalfa-grass mixtures. The primary objective of this study was to compare the effects of cutting management systems on herbage yield and stand persistence of orchardgrass (Dactylis glomerata L.) and an alfalfa (Medicago sativa L.)-orchardgrass mixture from various rates and frequencies of dairy slurry application. A randomized complete block design with treatments in a sub-subplot arrangement with four replicates was used. The main plot consisted of 2 cutting management systems (4 and 5 annual cuttings). The subplots were 9 fertility treatments: 7 slurry rate and frequency of application treatments, one inorganic fertilizer treatment, and an unfertilized control. The split-split-plots were the two forage species: orchardgrass and alfalfa-orchardgrass mixture. The study was initiated after 1st cutting in 1995. Cumulative yields of the 2nd and subsequent cuttings of both orchardgrass and alfalfa-orchardgrass in 1995 were higher for the 5-cutting system than the 4-cutting system. The 1995 growing season was abnormally dry. In 1996, an abnormally wet year, the reverse was true, total herbage yields being higher for the 4-cutting system than the 5-cutting system. Species response to fertility rate/frequency treatments was different in both years. Higher application rates early in the season and carryover of nutrients from late season applications the previous year appear to be responsible for the yield increases of those fertility treatments having significant yield differences between the cutting management systems. The stand ratings of orchardgrass were not affected by cutting management. In the spring of 1997, however, the stand ratings of alfalfa-orchardgrass in the 4-cutting management system were significantly greater than the 5-cutting management system. The very high manure application rate significantly reduced the stand ratings of alfalfa-orchardgrass in the 5-cutting system.

• Herbal Formula Science
• /
• v.17 no.2
• /
• pp.175-186
• /
• 2009

The vasorelaxant effect of an extract of Lophatherum gracile Brongn (ELB) and its possible action mechanism were ascertained in aortic tissues isolated from rats. ELB relaxed endothelium-intact thoracic aorta in a dose-dependent manner. However, the induced vascular relaxation was abolished by removal in endothelium of the thoracic aorta. Pretreatment of endothelium-intact vascular tissues with $N^G$-nitro-L-arginine methyl ester (L-NAME) or 1H-[1,2,4]-oxadiazole-[4,3-$\alpha$]-quinoxalin-1-one (ODQ) significantly inhibited vascular relaxation induced by ELB. Moreover, ELB significantly increased cGMP production in aortic tissues, which was blocked by pretreatment with L-NAME or ODQ. The vasorelaxant effect of ELB was attenuated by tetraethylammonium (TEA), and glibenclamide. ELB-induced vasorelaxation was not blocked by atropine, propranolol, indomethacin, verapamil, and diltiazem. Taken together, the present study demonstrates that ELB dilates vascular smooth muscle via an endothelium-dependent NO-cGMP signaling pathway, which may be at least in part related with the function of $K^+$ channels.

• Molecules and Cells
• /
• v.40 no.8
• /
• pp.558-566
• /
• 2017

Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome. This study was subsequently conducted to examine whether superovulation treatment can preserve ultrastructural integrity and developmental competence of oocytes following oocyte activation and embryo culture. A randomized study using mouse model was designed and in vitro development (experiment 1), ultrastructural morphology (experiment 2) and functional integrity of the oocytes (experiment 3) retrieved after PMSG/hCG injection (superovulation group) or not (natural ovulation; control group) were evaluated. In experiment 1, more oocytes were retrieved following superovulation than following natural ovulation, but natural ovulation yielded higher (p < 0.0563) maturation rate than superovulation. The capacity of mature oocytes to form pronucleus and to develop into blastocysts in vitro was similar. In experiment 2, a notable (p < 0.0186) increase in mitochondrial deformity, characterized by the formation of vacuolated mitochondria, was detected in the superovulation group. Multivesicular body formation was also increased, whereas early endosome formation was significantly decreased. No obvious changes in other microorganelles, however, were detected, which included the formation and distribution of mitochondria, cortical granules, microvilli, and smooth and rough endoplasmic reticulum. In experiment 3, significant decreases in mitochondrial activity, ATP production and dextran uptake were detected in the superovulation group. In conclusion, superovulation treatment may change both maturational status and functional and ultrastuctural integrity of oocytes. Superovulation effect on preimplantation development can be discussed.

• Journal of Agricultural Extension & Community Development
• /
• v.31 no.2
• /
• pp.41-56
• /
• 2024

This study aims to examine the causal relationshps between self-efficacy and job satisfaction of young farmers by verifying the mediating effect of self-efficacy in the relationship between job ability and job satisfaction of young farmers, and to improve the job ability necessary for agriculture, and to provide implications for the development and operation of curriculum that meets the needs of farmers. To this end, an online survey was conducted on 300 young farmers under the age of 40 among 4-H members in Korea from June 17 to 28, 2022. The results of the analysis to find out the mediating effect of self-efficacy in the relationship between job ability and job satisfaction of young farmers are as follows. First, it was verified that self-efficacy plays a partial mediating role in affecting job satisfaction by verifying whether the job ability of young farmers mediates self-efficacy in the relationship between job satisfaction and job satisfaction. It can be said that the higher the job ability, the more positive the job satisfaction and the more positive the self-efficacy, which ultimately has a positive effect on job satisfaction. Second, as a result of verifying whether self-efficacy is mediated in the relationship between job satisfaction and job satisfaction by sub-factors of job competency in detail, self-efficacy plays a partial mediating role between agricultural production ability, agricultural machine management ability, and job satisfaction, and agricultural management ability and agricultural information utilization ability do not have a direct significant effect on job satisfaction.