• Food Science and Preservation
• /
• v.20 no.6
• /
• pp.871-876
• /
• 2013

The purpose of this study was to investigate antimicrobial effectiveness of Omija (Schizandra chinensis Baillon) treated with $ClO_2$ (chlorine dioxide) concentration (10, 15, 20, 25 and 30 ppm), washing time (30, 60 and 90 sec) and multiple proportion (x1, x2, x3 and x4). The seven groups were divided into control (Omija without washing water treatment), W-T (Omija treated by tap water ($20{\pm}1^{\circ}C$) for 30 seconds), $ClO_2$-10 (Omija treated by 10 ppm $ClO_2$), $ClO_2$-15 (Omija treated by 15 ppm $ClO_2$), $ClO_2$-20 (Omija treated by 20 ppm $ClO_2$), $ClO_2$-25 (Omija treated by 25 ppm $ClO_2$), $ClO_2$-30 (Omija treated by 30 ppm $ClO_2$), and then they were detected number of total aerobic bacteria, yeast and mold. The rate of inactivation was found, for microorganisms of total aerobic bacteria, yeast and mold, to increase with a increase of $ClO_2$ treatment concentration and multiple proportion. No total aerobic bacteria, yeast and mold in $ClO_2$-30 sample treated for 30 sec, $ClO_2$-15 treated for 60 sec and $ClO_2$-10 treated for 90 sec were detected, and in $ClO_2$-30 Omija with multiple proportion ${\times}1$ (Omija : 30 ppm $ClO_2$ solution ratio was 1:1 (w/w)), $ClO_2$-20 with ${\times}2$ (Omija : 20 ppm $ClO_2$ solution ratio was 1:2 (w/w)) and $ClO_2$-15 with ${\times}4$ (Omija : 15 ppm $ClO_2$ solution ratio was 1:4 (w/w)) respectively.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.3
• /
• pp.400-409
• /
• 2015

This study examined the plant growth and development characteristics of leafy vegetables on the hydroponic substrates of waste wood chips, radiate pine chips, and mat type of waste wood chips. The bulk density of waste wood chips and radiata pine chips were $0.2g/cm^3$ and $0.16g/cm^3$, respectively. The moisture retention properties of both the radiata pine chips and waste wood chips were found to be similar but not better than those of the control rock wool and coco peat hydroponic substrates. The moisture retention property of the mat type was found to be the lowest. The chemical analysis of waste wood hydroponic substrates (w/v) was as follows.; The pH was 6.59, electric conductivity was 6.76 dS/m, total nitrogen content was 0.5%, C/N ratio was 113%, phosphorus was 10.1 ppm, potassium was 77 ppm, calcium was 531 ppm, magnesium was 49 ppm, and sodium was 96 ppm. The results from the radiata pine chemical analysis showed that it had a pH of 5.29, electric conductivity of 4.49 dS/m, total nitrogen content of 0.32%, C/N ratio of 180%, phosphorus of 6.4 ppm, potassium of 83 ppm, calcium of 97 ppm, magnesium of 29 ppm, and sodium of 59 ppm. Except for the plants grown in mat type, the developmental characteristics of the plants grown in rock wool and coco peat hydroponic substrates were similar. Based on the results of the experiment, waste wood resources may possibly be used as an organic solid medium in place of the existing rock wool and coco peat medium.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.34 no.6
• /
• pp.776-783
• /
• 2005

To develop functional kimchi which had anti-obesity effect, garcinia cambogia extract containing $51.46\%$ hydroxy citric acid (HCA) was used as a sub-ingredient of American preferred kimchi (APK). The APK added to garcinia cambogia extracts of 0.5$\%,\;1.0\%,\;1.5\%\;and\;2.0\%$ were prepared, and fermentation characteristics and anti-obesity effect of those kimchi were investigated. The pH of APK added to garcinia cambogia extracts (APKH) was low as the amount of garcinia cambogia extract increased at initial stage of fermentation but the pH of those kimchi showed similar values after optimum ripened stage. The number of Lactobacillus sp. and Leuconostoc sp. were small as the amount of garcinia cambogia extract increased while the period was delayed that the number of Lactobacillus sp. and Leuconostoc sp. attained to maximum. In Hunter's color values of APKH, lightness and redness decreased as the amount of garcinia cambogia extracts increased while yellowness increased. Sensory scores in overall acceptance, taste and texture of APKH evaluated by Americans as sensory panels were similar until the addition amount of garcinia cambogia extract was $1.5\%$, therefore the garcinia cambogia extract of $1.5\%$ was determined as tile amount adding to APK. The secretions of glycerol and leptin as a key signalling factor for anti-obesity effect were examined in APK and APKH added to garcinia cambogia extract of $1.5\%$. There were no significant differences in the glycerol secretion when adipocytes were treated with APK and APKH extracts. However, leptin secretion in the adipocytes treated with APKH extract was significantly decreased compared to that of control (p<0.05).

• Korean Journal of Ecology and Environment
• /
• v.34 no.4 s.96
• /
• pp.298-309
• /
• 2001

The purpose of this study was to evaluate the filtering-feeding effect of a freshwater mussel (Corbicula leana) on the phytoplankton communities in two lakes with different trophic conditions between June and September, 2000. Manipulation experiments were conducted with two treatments (the control and mussel addition), and each established in duplicate 10 l chambers. Both ambient nutrient (TN, TP) and chlorophyll-a concentrations were significantly (p<0.01) higher in Lake Ilgam than Lake Soyang. Cyanophytes (Microcystis, Oscillatoria, Lyngbya and Dactylococcopis) consistently dominated algal community in Lake llgam, while flagellated algae (Dinobryon divergence, Mallomonas, Rhodomonas) and cyanophytes (Microcystis)dominated in Lake Soyang. The net exponential death rate ($R\;=\;day^{-1}$) of total phytoplankton in the mussel treatment ranged $1.70{\sim}7.39$ and $0.38{\sim}1.64$ in Lakes Soyang and Ilgam, respectively. Mean filtering rate standardized by mussel AFDW ($ml\;mgAFDW^{1}\;h^{-1}$) was much higher in Lake Soyang ($1.70{\sim}3.06$) than in Lake Ilgam($0.24{\sim}0.88$0.24~o.88). Estimating FR per mussel, 1 mussel filtered $1.6{\sim}7.8\;l$ per day and $1.7{\sim}3.0\;l$ per day in Lakes Soyang and Ilgam, respectively. Based on tile C-flux tobiomass ratio, Corbicula leana consumed $0.8{\sim}4.4$ fold of phytoplankton standing stock in Lake Soyang, and $0.4{\sim}1.6$ fold in Lake Ilgam per day. Mussel feeding resulted in increase of SRP concentration by $30{\sim}50%$, compared with the control. The results of this study suggest that filter-feeding activity of Corbicula leana varies depending on the phytoplankton density and community composition. The high seston consumption rate of Corsicuja Jeaua even in a eutrophic lake suggests that biomanipulation approach using filter-feeding mussels can be used far wate rquality management in small eutrophic reservoirs.

• Journal of Marine Bioscience and Biotechnology
• /
• v.1 no.2
• /
• pp.91-97
• /
• 2006

For efficient removal of nitrogenous compounds produced in recirculating aquaculture system, the N removal characteristics of immobilized mixed microorganisms were investigated at various mixing ratios of photosynthetic bacteria (PSB) immobilized in PVA beads or CTA cubes and ammonium utilizing bacteria (AUB) immobilized in PVA beads. On the optimal medium of AUB, the maxium gas production rate was obtained at the mixing ratio of 10:40 (PSB:AUB), and the gas production rate increased as the portion of AUB beads in the mixed beads increased. When the mixing ratios of PSB:AUB beads were 50:0, 40:10, 25:25 and 10:40, the final pHs were measured to be 6.29, 6.01, 5.69 and 5.13, respectively. On the optimal medium of PSB, however, the volume and the rate of gas production decreased remarkably as the portion of AUB beads in the mixed beads increased. The final pH was measured to be approximately 6.5, regardless of the mixing ratio. In the reactions by the mixed culture of PSB cubes and AUB beads, all results showed the same tendency of those by the mixed culture of PSB and AUB beads, but the volume and the rate of gas production decreased remarkably, even with 0.2ml of gas production in control. From all the results, the use of mixed PSB and AUB beads at the ratio of 10:40 seems to be efficient to remove nitrogenous compounds in wastewater from recirculating aquaculture system.

• Journal of agriculture & life science
• /
• v.44 no.6
• /
• pp.111-119
• /
• 2010

Salmonella spp. are one of major pathogens for zoonosis in worldwide, and can replicate within host cells and generally cause enterocolitis and foodborne poisoning which represents a considerable public health burden. The present study was designated to investigate the safty for host cells, antibacterial effects of Saururus chinensis Baill water extract (SCWE) on pure culture and infection with Salmonella enterica serovar Typhimurium (S. typhimurium) in murine derived macrophage RAW 264.7 cells. The different treatment of SCWE concentration (1, 10 or $100{\mu}g/ml$) did not show any cytotoxic effect to RAW 264.7 cells for 24 h incubation. In determination of antibacterial activity of SCWE against S. typhimurium, bacterial viability was markedly decreased compared to SCWE-untreated control. In RAW 264.7 cells, SCWE significantly induced morphological change (p<0.05). In infection assay of S. typhimurium in RAW 264.7 cells pretreated with $100{\mu}g/ml$ of SCWE, which are non-cytotoxic concentration, bacterial uptake ability of macrophage was increased corresponding with morphological change, whereas bacterial survival rates within macrophage was markedly reduced comparing to that of SCWE-untreated control. Furthermore, nitric oxide (NO) production in SCWE-treated cells was slightly decreased until 24 h post infection. Taken together, these findings demonstrated that SCWE have the antibacterial activity for S. typhimurium and the protective effects against S. typhimurium infection through activating murine macrophage independent on NO, suggesting that SCWE were beneficial on the disease caused by intracellularly replicating pathogens as a safe alternatives of conventional chemotherapies.

• Asian-Australasian Journal of Animal Sciences
• /
• v.12 no.5
• /
• pp.758-765
• /
• 1999

Comparative studies of the effects of rates and frequency of application of dairy slurry on herbage yield and stand persistence of alfalfa and various forage grasses have not previously been conducted. The results being reported here are part of a larger study having a primary objective of comparing the effectiveness of alfalfa (Medicago sativa L.), various grasses and alfalfa-grass mixtures for utilizing nutrients from applied dairy slurry. The objectives of this part of the study were to evaluate the effects of various rates and frequencies of application of slurry on herbage yield and stand persistence of orchardgrass (Dactylis glomerata L.), reed canarygrass (Phalaris arundinacea L.), and alfalfa-orchanrdgreass and alfalfa-reed canarygrass mixtures managed as a 4-cutting management system. A randomized complete block design with treatments in a split plot arrangement with four replicates was used. The main plots consisted of 9 fertility treatments: 7 slurry rate and time of application treatments, one inorganic fertilizer treatment, and an unfertilized control. The sub-plots consisted of the two grasses and two alfalfa-grass mixture mentioned above. Slurry was composed from stored solids scraped from the alleyways of a free-stall housing barn and water added to form a slurry having about 8% solids. Manure was pumped from a liquid spreader tank into 10.4 L garden water cans for manual application to the plots. Herbage yields within species were generally unaffected by various rates of application in the first production year. Herbage yields of grasses and alfalfa-grass mixtures the second year were generally not affected by frequency of application for the same rate of slurry applied. Slurry application resulted in greater herbage yield increases in grasses than alfalfa-grass mixtures in the 4-cutting management system. In general, herbage dry matter yields of grasses from the dairy slurry treatments equaled or exceeded yields from the inorganic fertilizer treatment. Stand ratings of grasses and alfalfa-grass mixtures were not changed by manure application rates. In this study, the highest rate of slurry ($967kg\;total\;N\;ha^{-1}$ in 1995 plus $2,014kg\;N\;ha^{-1}$ in 1996) was not detrimental to herbage yields or stand persistence of any of the species. It was concluded that applying dairy slurry to these cool-season grasses and alfalfa-grass mixtures managed in a 4-cutting system is an acceptable practice from the standpoint of herbage yield and satnd persistence and by doing so the utilization of inorganic fertilizers can be reduced.

• Journal of Embryo Transfer
• /
• v.25 no.3
• /
• pp.171-177
• /
• 2010

Plasminogen activators (PAs) are serine proteases that convert plasminogen to plasmin. The PA/plasmin system has been associated with a number of physiological processes such as fibrinolysis, ovulation and fertilization. Although correlations have been reported between reactive oxygen species (ROS) and oocyte maturation, the relationship between PA activity and ROS is unknown. The present study was undertaken to determine the effects of cumulus cells on PA activity in matured porcine oocytes under xanthine (X)-xanthine oxidase (XO) system. When oocytes were matured under the X-XO system, the proportion of oocytes remaining GV stage was higher (p<0.05) in oocytes without cumulus cells. The incidence of degenerated oocytes was higher (p<0.05) in the X+XO ($11.1{\pm}6.1$ and $21.6{\pm}3.4%$) than in the control group ($2.9{\pm}1.8$ and $4.0{\pm}1.6%$). The proportion of TUNEL-positive oocytes and activity of caspase-3 were higher (p<0.05) in cumulus-free oocytes and oocytes exposed to ROS. Tissue-type plasminogen activator-plasminogen activator inhibitor (tPA-PAI) and tissue-type plasminogen activator (tPA) activity were detected in oocytes that were separated from cumulus-oocytes complexs (COCs) at 44 h of maturation culture, and only tPA was produced in oocytes that were denuded before the onset of maturation culture. On the other hand, the activities of PA were increased (p<0.05) when oocytes were cultured under the X-XO system. The higher activity of tPA was observed in denuded oocytes (DOs) underwent apoptotic changes by oxidative stress. In COCs, however, tPA-PAI as well as tPA activity was detected and apoptotic changes such as DNA cleavage or caspase-3 activation were not observed. These results suggest that tP A may be relevant to apoptotic cell death in porcine oocytes by oxidative stress.

• Nuclear Engineering and Technology
• /
• v.24 no.2
• /
• pp.154-162
• /
• 1992

Two major goals for chemical cleaning on the secondary side of nuclear steam generators are to remove sludge effectively and to minimize corrosion of base metals. In this work, electrochemical and sludge dissolution behaviors have been investigated in order to find out which parameters are critical and important during a copper removal process for chemical cleaning and to evaluate safety aspects and effectiveness of two major copper removal processes developed commercially in foreign countries. Hydrogen peroxide is vert effective for the process to use EDTA, NH$_4$OH and EDA at 38$^{\circ}C$ to control the potential of copper in a potential range sood for copper sludge removal. Corrosion rates for carbon steel SA 285 Gr.C and Alloy 600 are very small during this process if it is controlled properly. However, the corrosion rate of SA 285 Gr.C will be increased greatly if its corrosion potential is maintained below -450mV. The process to use EDA and ammonium carbonate is effective at 6$0^{\circ}C$ to dissolve copper sludge if the corrosion potential of copper can be controlled above -200mV. However, it is very difficult to raise the corrosion potential of copper to this range by air blowing and stirring.

• Tuberculosis and Respiratory Diseases
• /
• v.75 no.1
• /
• pp.9-17
• /
• 2013

Background: In cancer cells, autophagy is generally induced as a pro-survival mechanism in response to treatment-associated genotoxic and metabolic stress. Thus, concurrent autophagy inhibition can be expected to have a synergistic effect with chemotherapy on cancer cell death. Monensin, a polyether antibiotic, is known as an autophagy inhibitor, which interferes with the fusion of autophagosome and lysosome. There have been a few reports of its effect in combination with anticancer drugs. We performed this study to investigate whether erlotinib, an epidermal growth factor receptor inhibitor, or rapamycin, an mammalian target of rapamycin (mTOR) inhibitor, is effective in combination therapy with monensin in non-small cell lung cancer cells. Methods: NCI-H1299 cells were treated with rapamycin or erlotinib, with or without monensin pretreatment, and then subjected to growth inhibition assay, apoptosis analysis by flow cytometry, and cell cycle analysis on the basis of the DNA contents histogram. Finally, a Western blot analysis was done to examine the changes of proteins related to apoptosis and cell cycle control. Results: Monensin synergistically increases growth inhibition and apoptosis induced by rapamycin or erlotinib. The number of cells in the sub-$G_1$ phase increases noticeably after the combination treatment. Increase of proapoptotic proteins, including bax, cleaved caspase 3, and cleaved poly(ADP-ribose) polymerase, and decrease of anti-apoptotic proteins, bcl-2 and bcl-xL, are augmented by the combination treatment with monensin. The promoters of cell cycle progression, notch3 and skp2, decrease and p21, a cyclin-dependent kinase inhibitor, accumulates within the cell during this process. Conclusion: Our findings suggest that concurrent autophagy inhibition could have a role in lung cancer treatment.