• Asian-Australasian Journal of Animal Sciences
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• 제21권2호
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• pp.190-197
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• 2008

This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences in sperm motility and normal acrosome between boar A and B at 1 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively. However, sperm motility and normal acrosome from 2 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively, were higher for boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher for boar A than boar B. The sperm viability and ATP concentration according to storage period of liquid semen at $17^{\circ}C$ and $4^{\circ}C$ were higher for boar A than boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.

• Journal of Biosystems Engineering
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• 제33권6호
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• pp.384-389
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• 2008

Three prototype chestnut harvesters were constructed and evaluated their chestnut collection ability and field efficiency. Air-lock paddle system successfully picked up all loose material, and pick up efficiency was about 56 kg/h. Power required to operate this system was evaluated to be 8.7 kW with an air flow rate of $32.6\;m^3/min$. A radial blade type blower with 0.41 m impeller diameter was considered to be a minimum size for this system. For the auger system, air was sucked into the cylinder as the hinged flat cover began to be opened by the material pushed by the auger, and the empty burrs flew back to the container through the space between auger flights and collected in the bottom of the container. It was considered to add a device to prevent air from flowing back or to use the back flowing air for separation of burrs and nuts inside the container. The venturi system could not pick up chestnuts, as they only carried part way up to the suction hose. Consideration was given to an idea that the venturi could be used as a cleaning and separation mechanism for containers filled with both empty burrs and good nuts. A minimum vacuum of 129 mm wg was required to pick up chestnuts, and the corresponding inlet air velocity was 19.3 m/s. 104 mm of vacuum, which was about 81 % of that required for nuts, was enough to pick up burrs with nuts inside. Also, empty burrs with higher moisture content recorded the same pressure as for the burrs with nuts.

• Journal of Veterinary Science
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• 제23권6호
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• pp.68.1-68.8
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• 2022

Background: Studies on anesthetized dogs regarding pulse pressure variation (PPV) are increasing. The influence of respiratory rate (RR) on PPV, in mechanically ventilated dogs, has not been clearly identified. Objectives: This study evaluated the influence of RR on PPV in mechanically ventilated healthy dogs after hemorrhage. Methods: Five healthy adult Beagle dogs were premedicated with intravenous (IV) acepromazine (0.01 mg/kg). Anesthesia was induced with alfaxalone (3 mg/kg IV) and maintained with isoflurane in 100% oxygen. The right dorsal pedal artery was cannulated with a 22-gauge catheter for blood removal, and the left dorsal pedal artery was cannulated and connected to a transducer system for arterial blood pressure monitoring. The PPV was automatically calculated using a multi-parameter monitor and recorded. Hemorrhage was induced by withdrawing 30% of blood (24 mL/kg) over 30 min. Mechanical ventilation was provided with a tidal volume of 10 mL/kg and a 1:2 inspiration-to-expiration ratio at an initial RR of 15 breaths/min (baseline). Thereafter, RR was changed to 20, 30, and 40 breaths/min according to the casting lots, and the PPV was recorded at each RR. After data collection, the blood was transfused at a rate of 10 mL/kg/h, and the PPV was recorded at the baseline ventilator setting. Results: The data of PPV were analyzed using the Friedman test followed by the Wilcoxon signed-rank test (p < 0.05). Hemorrhage significantly increased PPV from 11% to 25% at 15 breaths/min. An increase in RR significantly decreased PPV from 25 (baseline) to 17%, 10%, and 10% at 20, 30, and 40 breaths/min, respectively (all p < 0.05). Conclusions: The PPV is a dynamic parameter that can predict a dog's hemorrhagic condition, but PPV can be decreased in dogs under high RR. Therefore, careful interpretation may be required when using the PPV parameter particularly in the dogs with hyperventilation.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제3권1호
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• pp.13-22
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• 2022

In this study, we investigated the water quality and fish community of the Gudam Wetland, a riverine wetland in the middle-upper reaches of the Nakdong River, during March-October 2020. The main results were as follows: average annual flow rate: 45.0±23.7 m³/s, flow velocity: 0.4±0.3 m/s, water depth: 1.4±0.4 m, water temperature: 17.5±0.8℃, pH: 7.8±0.2, electrical conductivity: 121.6±19.0 ㎲/cm, dissolved oxygen concentration: 11.4±0.9 mg/L, suspended solids concentration: 3.8±2.0 mg/L, and the water quality was classified as Ia (very good). A total of 754 individual fish belonging to 4 orders, 7 families, and 19 species were investigated. Cyprinidae was the dominant group, with 13 species. The dominant species was Zacco platypus (39.3%), followed by Pseudogobio esocinus (17.5%). There were 8 (42.1%) endemic Korean species and 1 exotic species, Micropterus salmoides. Four species were carnivores, six were insectivores, and nine were omnivores. Regarding tolerance to environmental changes, 6 species were tolerant, 11 had intermediate tolerance, and 2 were sensitive. Fish community analysis revealed dominance of 0.57, diversity of 2.04, evenness of 0.69, and richness of 2.72, indicating a diverse and stable fish community. The fish assessment index showed that the assessment class was B (average 62.5), which was higher than that of major streams of the Nakdong River (class C). For sustainable conservation of the Gudam Wetland, management strategies such as minimizing aggregate collection and preventing inflow of non-point pollutants are required.

• 한국수정란이식학회지
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• 제24권1호
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• pp.39-45
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• 2009

This study was carried out to evaluate the nuclear, cytoplasmic maturation and developmental potential of bovine oocytes selected by brilliant cresyl blue (BCB) as indirect measurement of oocytes growth phase. Cumulus-oocyte complexes (COCs) were collected from 2 to 8 mm follicles from slaughterhouse Hanwoo ovaries. The COCs were divided into stained cytoplasm to blue (BCB+) and unstained (BCB-) according to their ooplasm BCB coloration stained by $26{\mu}m$ of BCB after 90 min. Selected COCs were cultured in a TCM 199 for 18 to 26 h. Nuclear maturation and total cell number was evaluated after in vitro maturation (IVM) or in vitro culture (IVC) using $10{\mu}g/ml$ Hoechst 33342, and cytoplasmic maturation was evaluated by intracellular glutathione (GSH) assay before (0 h) and after (24 h) IVM. The oocyte diameters were not differed significantly between BCB+ ($157.4{\pm}5.8{\mu}m$) and BCB+ ($149.0{\pm}31.0{\mu}m$) groups (p>0.05). However, the proportion of metaphase II oocytes in BCB+ group was significantly higher than BCB- group after IVM (p<0.05). GSH content of BCB+ group oocytes was significantly higher than that of BCB- group just after collection ($7.3{\pm}0.6$ vs. $4.8{\pm}0.6\;pmol/oocyte$, p<0.05), but not varied after IVM($13.1{\pm}0.9$ and $12.6{\pm}2.5\;pmol/oocytes$ for BCB+ and BCB- respectively; p>0.05). The proportion of blastocyst formation and total cell number in BCB+ group (23.5% and $105.5{\pm}28.6$) was significantly higher than that in BCB- (9.8% and $72.4{\pm}26.1$; p<0.05). The results indicate that BCB+ group oocytes may provide a cellular and functional basis for the greater developmental competence in Korean Native Cow (KNC) oocytes.

• 한국응용곤충학회지
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• 제18권3호
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• pp.127-132
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• 1979

본 실험은 몇가지 우화기구를 사용하여 기구형에 따른 우화상내 온도변화가 솔잎혹파리의 우화소장에 미치는 영향 및 유충의 월동기간중의 온도변화와 우화소장간의 관계를 알아 보기 위하여 각처리별 우화소장을 조사하여 다음과 같은 결과를 얻었다. 1) 우화기구별 상내 최고온도는 현저한 차리를 나타내어 대조구에 비하여 Vinyl형은 월평균 $15^{\circ}C$가 높았고 다른 처리구에 있어서도 $30^{\circ}C\~12^{\circ}C$가 높았는데 다른 처리구에 있어서도 $30^{\circ}C\~12^{\circ}C$가 높았는데 표준형의 순이었다. 2) 처리별유충의 월동기간중의 최저온도분포는 현저한 차이를 나타내지 않았다. 3) Vinyl형 처리구에서는 우화최성일이 5월 15로 대조구에 비하여 20일 앞당겨했고 표준형구와 Cage-50구도 14일 빨라졌다. 4) Funnel형 처리구에 있어서는 최고온도 및 적산온도의 분포가 대조구에 비하여 높았음에도 불구하고 우화최성일은 6일이 지연되었다. 5) 유충의 월동기간중의 온도처리효과는 12월중순과 3월중순의 최고온도의 상승이 우화최성일을 앞당기는 효과를 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제8권5호
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• pp.517-522
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• 1998

The cry1 gene content of a collection of Bacillus thuringiensis strains, which include new isolates from Malaysia and Indonesia, was determined by a multiplex PCR using a set of eight oligonucleotide forward primers specific to cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1Ca, cry1Da, cry1Ea, and cry1Fa genes, and two reverse primers, one specific to cry1Ab and the other common to the remaining cry1 genes. Two-thirds of the 59 strains screened were cry1 positive and contained one to four different genes. The cry gene profiles correlated well with toxicities of the strains to lepidopteran insects. The method can be used for rapid screening of a large number of new isolates as the total DNA extracted by boiling cells from single colonies can be used directly in the PCR. However, it is not suitable for follow-up monitoring of specific commercial strains after application in the field as the PCR product profiles of these strains could not be differentiated from those of new isolates.

• Asian-Australasian Journal of Animal Sciences
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• 제2권4호
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• pp.583-590
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• 1989

Increasing levels of ammonia-N in the rumen fluid used for in vitro incubation were achieved by supplementation of the ration of the donor cows with urea and by addition of urea either with or without glucose to the rumen fluid after collection. The ration of the donor animals consisted of wheat straw (80%) and maize silage (20%). During the second half of the experiment the basal ration was supplemented with a mineral mixture. Wheat straw, Guinea grass and two rice straw varieties were incubated with the various kinds of rumen fluid. Parameters studied were: solubility, apparent organic matter disappearance after 48 hours of incubation ($OMD_{48}$), rate of organic matter degradation from 0 to 24 hours of incubation ($k_1$) and from 24 to 95 hours ($k_2$). The concentration of ammonia-N in the rumen fluid at which 95% of the maximal $OMD_{48}$ and k1 were reached (88.2 and 100.0 mg/l) were independent of the feed. With regard to the $k_2$ the required ammonia-N concentration to reach 95% of the maximal $k_2$ differed per feed. Mineral supplementation increased the OMD48 and $k_1$, but not the solubility and $k_2$. Glucose addition in combination with urea had no beneficial effect compared to urea supplementation alone.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 1998년도 공동 심포지엄 및 추계학술발표회
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• pp.225-231
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• 1998

Open dump causes groundwater and soil contamination by leachate, air pollution by LFG (Landfill Gas). In this paper, in order to improve landfill researches which have been done about reduction of high leachate level and LFG collection in the Kimpo landfill separately, the effect of simultaneous flowing of leachate and LFG has been Studied. The HYLGS (Hanyang Leachate Gas Simulator) used in this study is a 3D, 2-phase, transient FDM model which can be applied to venting trenches in a landfill. From present numerical analysis it can be concluded that all the pressures of the Kimpo landfill grid system are almost the same and their maximum value in the center grid block of the system is approximately 26 m $H_2O$ (2.52 atm), that because the pressures of venting trench layer situated in the middle of the landfill have the lowest values and equal with air pressure, the venting trenches play an important role in landfill stabilization, that the flow of gas will be more difficult as time goes by owing to the increase of LGR(Leachate and gas ratio).

• 한국IT서비스학회:학술대회논문집
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• 한국IT서비스학회 2003년도 춘계학술대회
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• pp.591-597
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• 2003

모바일 서비스는 공급사슬의 모든 단계와 연관되어 있는데, 특히 재고 주문관리, 주문 충족, 구매, 물류와 모니터링 분야와 밀접히 관련시켜 볼 수 있다. 기본적으로 모바일 서비스의 핵심은 참가자들 간에 정보의 공유로서 이를 통해서 공급사슬의 지각과 반응의 효율을 높일 수 있다. 모바일 서비스의 장점은 편재성(ubiquity), 속도, 추적, 위치확인, 개인화와 안전성에 있다. 공급사슬의 3가지 중요한 요소는 속도, 변화 및 가시성을 들 수 있는데, 모바일 서비스는 이 세가지 분야를 충족시킬 수 있다. 본 연구의 목적은 모바일 서비스를 이용해서 wireless 공급 웹을 모델링 하고자 한다. 재고관리, 원격 진단, 현장 작업자와의 통신, 추적과 모니터링을 포함한 고객요구 충족, 재고의 가시성, 실시간 shop floor data collection, 자산 추적 및 창고관리분야에 적용가능한 고객중심의 공급 웹을 모델링하고 RFID를 이용해서 창고관리 문제에 대한 프로토타입을 제시하고자 한다.