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Effects of Salt Concentrations on Accumulation of Pigments in Cell Suspension Cultures of Vitis vinifera and Phytolacca americana L. (포도와 미국자리공의 세포현탁배양계에 있어서 배지내 무기염 농도가 색소축적에 미치는 영향)

  • In, Jun Gyo;Lee, Young Bok;Choi, Kwan Sam
    • Korean Journal of Agricultural Science
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    • v.20 no.1
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    • pp.34-42
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    • 1993
  • Effects of salt concentrations on the cell growth and the pigment accumulation were investigated in cell suspension culture of Vitis vinifera and Phytolacca americana L.. The growth pattern of vine cell in control was showed the normal exponential growth pattern, but in the dilution media delay the exponential growth pattern from 4 to 8 days after culture. Maximal accumulation of anthocyanin was observed at 12 days after culture in all treatments. In cell suspension culture of Phytolacca, accumulation of betacyanin occurred in parallel with the cell growth pattern and maximal accumulation of betacyanin was observed after 8 days of culture. In the vine cell culture, the cell growth was showed the peak at 87.6mM of sucrose in the medium and reduced at over this concentration. Maximal anthocyanin accumulation was showed at 146mM of sucrose. In the higher concentrations of sucrose, the cell growth was rapidly decreased, but the accumulation of anthocyanin was not. Otherwise, in case of Phytolacca cell culture, betacyanin accumulation was showed in parallel with the cell growth increased with sucrose concentration. It was suggested that the anthocyanin of vine and the betacyanin of Phytolacca were controlled by different mechanisms.

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Structural characteristics of a red ginseng acidic polysaccharide rhamnogalacturonan I with immunostimulating activity from red ginseng

  • Lee, Sue Jung;In, Gyo;Han, Sung-Tai;Lee, Mi-Hyang;Lee, Jong-Won;Shin, Kwang-Soon
    • Journal of Ginseng Research
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    • v.44 no.4
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    • pp.570-579
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    • 2020
  • Background: Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides. But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated. Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng. Methods: A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100. Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity. The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis. Results: Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α. The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis. RGP-AP-I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%). RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan. Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I). Conclusion: we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(1→4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β-3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.

The Effect of NaCI on the Growth and Ginsenoside Production from Ginseng Hairy Root (인삼모상근의 생장과 Ginsenoside 생산에 미치는 NaCl의 영향)

  • Kim, Yu-Jin;Sim, Ju-Sun;;Lee, Chung-Hyae;In, Jun-Gyo;Lee, Bum-Soo;Yang, Deok-Chun
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.94-99
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    • 2008
  • Korean ginseng (Panax ginseng C.A. Meyer) is very difficult to obtain stable production of qualified ginseng roots because of variable stresses in soil environments. High salt concentrations in the ginseng nursery soil environment of Korea is one of important reducing factors for the stable production of quality ginseng. These studies were accomplished to identify the growth rate and production of ginsenoside from ginseng hairy root against NaCI. In the MS liquid culture, the highest contents and productivity of ginsenosides were appeared at 4 week after onset of the treatment of 0.1 M NaCI. And 0.24 M NaCI was more effective on the growth of ginseng hairy root under light condition than dark condition. Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, NaCI acts as a kind of stress as well as elicitor for production of ginsenosides.

Resident Involvement Analysis of New Town Landscape Architecture Construction - Focused on the Gyeonggi GwangGyo District - (택지개발지구 조경공사의 주민관여 분석 - 경기도 광교지구를 중심으로 -)

  • Oh, Jeong-Hak
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.6
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    • pp.51-59
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    • 2016
  • The purpose of this study is to improve interaction with the construction subject by analyzing the contents and contents of users' involvement in landscaping works. For this purpose, this study selected the Gwanggyo Residential Land Development District Public Landscape Project in Suwon, Gyeonggi Province. For four years before and after the completion, the opinions of tenants were used as research data. Both qualitative and quantitative analyses of 412 complaints received by the project implementation office and local government were conducted. As a result, first, the main purpose of suggesting opinions was 'demanding and expressing complaints', and there were many 'parks' and 'rivers'. In terms of content, "quality" was the most pointed out, but many kinds of trees, such as tree planting, ecological river construction, and pavement construction were also mentioned. Second, the extraction of key words from content analysis was the most common method. Followed by 'additional foodstuff' and 'moving to the toilet and management building'. Much of the point of view about dead wood has continued to be conspicuous in the process of waiting to be dealt with at the time of transplanting. Third, the validity of the contents of the complaints was evaluated as a five - point scale. Therefore, the opinions raised were unreasonable, but overall, there were more complaints with certain objectivity.

Synthesis and Crystal Structure of Zinc Iodide in the Sodalite Cavities of Zeolite A (LTA)

  • Kim, Seok-Han;Park, Man;Son, Young-Ja;Lee, Hyung-Joo;Jeong, Gyo-Cheol;Bae, Myung-Nam;Lim, Woo-Taik
    • Bulletin of the Korean Chemical Society
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    • v.28 no.4
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    • pp.567-573
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    • 2007
  • The crystal structure of ZnI2 molecule synthesized in zeolite A (LTA) has been studied by single-crystal X-ray diffraction techniques. A single crystal of |Zn6|[Si12Al12O48]-LTA, synthesized by the dynamic ion-exchange of |Na12|[Si12Al12O48]-LTA with aqueous 0.05 M Zn(NO3)2 and washed with deionized water, was placed in a stream of flowing 0.05 M KI in CH3OH at 294 K for four days. The resulting crystal structure of the product (|K6Zn3(KI)3(ZnI2)0.5|[Si12Al12O48]-LTA, a = 12.1690(10) A) was determined at 294 K by single-crystal X-ray diffraction in the space group Pm3m. It was refined with all measured reflections to the final error index R1 = 0.078 for 431 reflections which Fo > 4σ (Fo). At four crystallographically distinct positions, 3.5 Zn2+ and nine K+ ions per unit cell are found: three Zn2+ and five K+ ions lie on the 3-fold axes opposite 6-rings in the large cavity, two K+ ions are off the plane of the 8-rings, two K+ ions are recessed deeply off the plane of the 8-rings, and the remaining a half Zn2+ ion lie on the 3-fold axes opposite 6-rings in the sodalite cavity. A half Zn2+ ion and an I- ion per unit cell are found in the sodalite units, indicating the formation of a ZnI2 molecule in 50% of the sodalite cavities. Each ZnI2 (Zn-I = 3.35(5) A) is held in place by the coordination of its one Zn2+ ion to the zeolite framework oxygens and by the coordination of its two I- ions to K+ ions through 6-rings (I-K = 3.33(8) A). Three additional I- ions per unit cell are found opposite a 4-ring in the large cavity and form a K3I2+ and two K2ZnI3+ ionic clusters, respectively.

Speech Outcome after Closure of Oronasal Fistula Following Cleft Palate Repair: A report of a case (구개봉합술 후 발생한 구비강누공의 폐쇄 후 말소리 결과 : 증례보고)

  • Seo, Min-Gyo;Kim, Da-Wa;Kim, Eun-Ju;Yoon, Bo-Keun;Kim, Seong-Il;Leem, Dae-Ho;Ko, Seung-O;Moon, Seung-Young;Kim, Hyun-Ki;Shin, Hyo-Keun
    • Korean Journal of Cleft Lip And Palate
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    • v.12 no.1
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    • pp.1-6
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    • 2009
  • Oronasal fistula are a well-known complication of surgical treatment of cleft palate, occurring most frequently in the alveolus and hard palate. Previous reports have demonstrated that oronasal fistulas, particularly if greater than l cm in diameter, had an adverse effect on speech. The aim of this study was to demonstrate the relationship between the size of the fistula and the influence on velopharyngeal function. The site and size of the fistula were indicated on graph paper with calipers and measured in $mm^2$. Speech assessment was carried out using a Nasometer, VPI articulation differential test, spectrography. Patient whose fistulas affected their speech had significantly larger fistulas than those whose fistulas did not. The study shows that the larger the fistula, the greater the risk of hypernasality and nasal emission, but even small fistulas can cause speech problems. If obstruction of the nasal passage is eliminated in a patient with a previously asymptomatic fistula, it may result in a fistula becoming symptomatic, resulting in hypernasality and nasal emission. In conclusion, even small fistulas can influence speech production and should be considered before any treatment is planned. The study lends support to early closure of oronasal fistulas, particularly before pharyngeal flap surgery is contemplated.

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Evaluation of groundwater recharge rate for land uses at Mandae stream watershed using SWAT HRU Mapping module (SWAT HRU Mapping module을 이용한 해안면 만대천 유역의 토지이용별 지하수 함양량 평가)

  • Ryu, Jichul;Choi, Jae Wan;Kang, Hyunwoo;Kum, Donghyuk;Shin, Dong Suk;Lee, Ki Hwan;Jeong, Gyo-Cheol;Lim, Kyoung Jae
    • Journal of Korean Society on Water Environment
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    • v.28 no.5
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    • pp.743-753
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    • 2012
  • The hydrologic models, capable of simulating groundwater recharge for long-term period and effects on it of crops management in the agricultural areas, have been used to compute groundwater recharge in the agricultural fields. Among these models, the Soil and Water Assessment Tool (SWAT) has been widely used because it could interpret hydrologic conditions for the long time considering effects of weather condition, land uses, and soil. However the SWAT model couldn't represent the spatial information of Hydrologic Response Unit (HRU), the SWAT HRU mapping module was developed in 2010. With this capability, it is possible to assume and analyze spatio-temporal groundwater recharge. In this study, groundwater recharge of rate for various crops in the Mandae stream watershed was estimated using SWAT HRU Mapping module, which can simulate spato-temporal recharge rate. As a result of this study, Coefficient of determination ($R^2$) and Nash-Sutcliffe model efficiency (NSE) for flow calibration were 0.80 and 0.72, respectively, and monthly groundwater recharge of Mandae watershed in Haean-myeon was 381.24 mm/year. It was 28% of total precipitation in 2009. Groundwater recharge rate was 73.54 mm/month and 73.58 mm/month for July and August 2009, which is approximately 18 times of groundwater recharge rate for December 2009. The groundwater recharges for each month through the year were varying. The groundwater recharge was smaller in the spring and winter seasons, relatively. So, it is necessary to enforce proper management of groundwater recharge during droughty season. Also, the SWAT HRU Mapping module could show the result of groundwater recharge as a GIS map and analyze spatio-temporal groundwater recharge. So, this method, proposed in this study, would be quite useful to make groundwater management plans at agriculture-dominant watershed.

Effect of Substitution of Fermented King Oyster Mushroom By-Products Diet on Pork Quality during Storage

  • Chu, Gyo-Moon;Kang, Suk-Nam;Kim, Hoi-Yun;Ha, Ji-Hee;Kim, Jong-Hyun;Jung, Min-Seob;Ha, Jang-Woo;Lee, Sung-Dae;Jin, Sang-Keun;Kim, Il-Suk;Shin, Dae-Keun;Song, Young-Min
    • Food Science of Animal Resources
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    • v.32 no.2
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    • pp.133-141
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    • 2012
  • This study was carried out to investigate the effects of substitution of fermented king oyster mushroom (P. eryngii) by-products diet on pork meat quality characteristics, during the storage. A mixture of 40% king oyster mushroom by-products, 28% soybean meal and 20% corn was fermented for 10 d, and the basal diet was then substituted by the fermented diet mixture of up to 20, 50 and 80%, respectively. A total of 96 pigs were fed experimental diet (8 pigs per pen ${\times}$ 4 diets ${\times}$ 3 replication), and eight longissiumus (LD) per treatment were collected, when each swine reached to 110 kg of body weight. The Warner-Bratzler shear forces and cooking loss were significantly lowered in the treatments, while crude protein content and water holding capacity significantly (p<0.05) increased in the treatments than in the control group. The volatile basic nitrogen (VBN), at 1 d of storage, was lower in the treatments, while texture profiles and sensory evaluation did not differ between the control and the treatments (p>0.05). The pH, thiobarbituric acid reactive substances (TBARS), VBN and meat color in all treatments were increased as storage increased. Fermented king oyster mushroom by-products diet effects on lightness (CIE $L^*$), yellowness (CIE $b^*$) and chroma were determined, when LD muscles in T2 and T3 treatments were higher (p<0.05), up to 7 d (p<0.05). Therefore, the results indicate that the substitution of the fermented king oyster mushroom by-products diet to swine diet influenced the quality of the meat and it may be an economically valuable ingredient.

Additive Effects on Magnetic Properties in High Permeability Mn Zn Ferrite (고투자율 Mn-Zn 페라이트의 첨가물 효과에 따른 자기적 특성연)

  • Jeong, Gap-Gyo;Choe, Seung-Cheol
    • Korean Journal of Materials Research
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    • v.3 no.5
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    • pp.497-504
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    • 1993
  • Effects of $Ta_2O_5,ZrO_2$ and $SiO_2$ addition on magnetic properties of 0.02wt%$Bi_2O_3$ and 0 . 0 5 wt%$CaCO_3$ doped Mn-Zn ferrites(58.5mol% $Fe_2O_3$, 25.5 mol% ZnO) were investigated. E:lectrlcal resistivity and magnetic properties such as the initial permeability($\mu_i$), loss factor(tan$\delta$), coercive force Hc(m0c) were measured. With lncreasing $Ta_2O_5$ and $ZrO_2$ addition, the following effects were observed: I ) Decreasing of the average grain size; 2) lncreasing of the electrical resistivity and initial permeability; 3) Ilecreasmg of loss factor values. (very low loss esprcially at high frequency region) ; 4 ) Fine and uniform microsrructures were obtamed at O.lwt% nddecl samples. In case of $SiO_2$ addition, anomalous grain growth and degradation of magnetic properties were observed. The obtained maximum initial permeability value was 6260 at IOkHz. $25^{\circ}C$ from 0.02wt%$Bi_2O_3$. 0.05wt%$CaCO_3$, 0.lwt%$Ta_2O_5$ added sample, the corresponded relative loss factor (tan$\delta /\mu_i$)for the sample was $4.2 \times 10^{-6}$.

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Determination of Urban-Life Housing Price and Return Ratio by Location (도시형생활주택의 입지별 분양가격 및 수익률 결정요인)

  • Park, Jin-A;Woo, Chul-Min;Baik, Min-Seok;Shim, Gyo-Eon
    • The Journal of the Korea Contents Association
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    • v.12 no.11
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    • pp.469-481
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    • 2012
  • The demand for small-sized housing has been increasing due to the recession of real-estate price and the increase of small-sized households. Especially, the demand for affordable housing has been increasing since the style of housing and the location fits the lifestyle of small-sized household. In addition, many investors have been buying it because it has advertised as an investment property holding high-return ratio. However, an empirical analysis about the selling price and the return ratio has not been done yet. Therefore, the purpose of the research is having the empirical analysis based on the selling price and return ration by examining the affordable housing in Seoul. The urban-life housing more than 50 generations of the Seoul was irradiated for the analysis. And the linear regression analysis and PLS(Partial Least Square Regression) analysis was used for the empirical analysis. The result of analysis, based on the linear regression analysis, showed that factors including neighboring housing price and subway catchment area have a significant effect to the determinant factors of housing price. The analysis for return ratio showed neighboring housing price, subway catchment area and amenities affects the ratio. Especially, the fault of using small sample was covered by using the partial least square regression in this research.