• Parasites, Hosts and Diseases
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• 제34권2호
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• pp.107-112
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• 1996

참굴큰입흡충(Gymnophalloides seoi)의 국내 분포상황을 알기 위하여 동해안 남해안 및 서해안 등 여러 해안 지역에서 참굴을 채집한 후 참굴큰입흡충 피낭유충 감염상황을 조사하였다. 전라남도 신안군의 13개 면과 신안군 외 14개 군(경기도, 전라북도, 전라남도, 경상남도, 경상북도, 강원도)의 씨개 면을 대상지역으로 하였고. 굴의 피낭유충 감염 여부 및 감염량을 조사하였다. 신안군 13개 면 중 압해면 및 안좌면을 포함한 10개 면의 굴에서 피낭유충 감염이 확인되었고 지역별 감염률은 1.7-100%이었다. 그 중 압해면산 굴이 개체당 평균 785.9개의 피낭유충을 보유하고 있어 감염량이 가장 높았다 신안군 이외의 24개 면 중에서는 전라북도 부안군 변산면만 제외하고 모두 음성의 결과를 나타내었으며, 부안군 변산면에서는 굴 50개 중 1개에서 피낭유충 15마리가 검출되었다. 이번 연구 결과로 보아 참굴큰입흡충의 농후한 유행은 전라남도 신안군에 국한된 것으로 보이며, 전라북도 부안군 등 인근 지역에 약간의 유행이 있는 것으로 판단되었다.

• Parasites, Hosts and Diseases
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• 제33권1호
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• pp.61-64
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• 1995

참굴큰입흡충(Gymnophalloides seoi)은 참굴이 매개하는 우리 나라 고유의 인체기생 장흡충의 하나로서, 전남 신안군 도서 지방에서 큰 유행지가 발견된 바 있다. 이 흡충은 췌장 질환과의 관계가 의심되어 의학적 관심의 대상이 되고 있다 이번 증례 보고에서는 당뇨병과 동반된 흥미있는 G. seoi감염 2례를 발견하여 보고하고자 한다. 환자들은 당뇨병 치료를 위해 내원하였으며 내원시 대변검사에서 참굴큰입흡충류(gymnophallid)의 충란이 검출되었다 프라지콴텔 및 하제를 투여한 후 충체를 수집한 결과 G. seoi 충체 772마리 및 10마리가 각각 검출되었다. 증례 I은 62세 남자로 G. seoi의 유행지 부근인 목포에 거주하고 있었고, 증례 II는 인천에 거주하고 있으면서 참굴을 날로 즐겨 먹었다고 하였다. 이 증례들을 볼 때 G. seoi감염이 당뇨병과 어떠한 상관관계를 가지고 있을 가능성을 시사하였다.

• 한국패류학회지
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• 제15권2호
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• pp.121-125
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• 1999

The present study aimed to elucidate whether and intestinal fluke, Gymnophalloides sp. exist in Gogunsan-gundo located at western part of Korean Peninsula; about 100 km apart from Gunsan city, Jeollabuk-do, Korea. The survey was carried out Two times each in 1997 and 1998, examining stool samples by means of formalin-ether concentration method. Results are summarized as follows : of 411 fecal samples examined, Gymnophalloides sp. positives were 19 (8.9%); 9.5% inMunyeodo, 1% in Seonyudo, but none in Jangjado. The positive cases did not show any specific clinical complaints. Prevalence rate of Gymnophalloides seoi metacercariae from oysters, Crassostrea gigas were 80.8%

• Parasites, Hosts and Diseases
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• 제44권1호
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• pp.1-5
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• 2006

One coastal village in Haenam-gun and two in Yeongam-gun, Jeollanam-do were surveyed for intestinal parasite infections by fecal examination. The egg positive rates of Gymnophalloides seoi were high, $24.1\%$ (14/58) in Haenam-gun and $9.3\%$ (11/118) in Yeongam-gun. The egg positive rates of heterophyids, including Heterophyes nocens, and of Clonorchis sinensis were $10.3\%\;and\;6.9\%$ in Haenam-gun, and $14.4\%\;and\;8.5\%$ in Yeongam-gun, respectively. After praziquantel treatment and purgation, a total of 37,761 fluke specimens were recovered from 17 patients; 11 in Haenam-gun and 6 in Yeongam-gun. Gymnophalloides seoi was the most commonly recovered species, with 37,489 specimens in total (2,205 per person). Other recovered flukes included Heterophyes nocens, Stictodora fuscata, Heterophyopsis continua, Pygidiopsis sUmma, and undetermined species. These results indicate that the areas surveyed are new endemic foci of G. seoi.

• Parasites, Hosts and Diseases
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• 제44권1호
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• pp.87-89
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• 2006

A 55-year old Korean man, living in Mokpo-city, Jeollanam-do, Republic of Korea, visited a local clinic complaining of right upper quadrant pain and indigestion. At colonoscopy, he was diagnosed as having a carcinoma of the ascending colon, and thus, a palliative right hemicolectomy was performed. Subsequently, an adult fluke of Gymnophalloides seoi was incidentally found in a surgical pathology specimen of the lymph node around the colon. The worm was found to have invaded gut lymphoid tissue, with characteristic morphologies of a large oral sucker, a small ventral sucker, and a ventral pit surrounded by strong muscle fibers. This is the first reported case of mucosal tissue invasion by G. seoi in the human intestinal tract.

• Parasites, Hosts and Diseases
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• 제52권3호
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• pp.273-280
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• 2014

The changing patterns of goblet cell hyperplasia, intestinal epithelial cell turnover, and intestinal motility were studied in ICR and C57BL/6 mice infected with Gymnophalloides seoi (Digenea: Gymnophallidae). Whereas ICR mice retained G. seoi worms until day 7 post-infection (PI), C57BL/6 mice showed a rapid worm expulsion within day 3 PI. Immunosuppression with Depo-Medrol significantly delayed the worm expulsion in C57BL/6 mice. Goblet cell counts were increased in both strains of mice, peaking at day 1 PI in C57BL/6 mice and slowly increasing until day 7 PI in ICR mice. In C57BL/6 mice infected with G. seoi, newly proliferating intestinal epithelial cells were remarkably increased in the crypt, and the increase was the highest at day 1 PI. However, in ICR mice, newly proliferating intestinal epithelial cells increased slowly from day 1 to day 7 PI. Intestinal motility was increased in G. seoi-infected mice, and its chronological pattern was highly correlated with the worm load in both strains of mice. Meanwhile, immunosuppression of C57BL/6 mice abrogated the goblet cell proliferation, reduced the epithelial cell proliferation, and suppressed the intestinal motility. Goblet cell hyperplasia, increased intestinal epithelial cell turnover, and increased intestinal motility should be important mucosal defense mechanisms in G. seoi-infected C57BL/6 mice.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.705-708
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• 2019

Gymnophalloides seoi (Digenea: Gymnophallidae) is a human intestinal trematode contracted by eating raw oysters (Crassostrea gigas) in the Republic of Korea (=Korea). It has been known to be highly endemic in Aphae Island, Shinan-gun, Jeollanam-do (Province). However, recent epidemiological status of G. seoi has not been reported since the 1990s. In this study, we investigated the prevalence of G. seoi metacercariae in natural and cultured oysters collected from 3 islands and 2 coastal areas in western parts of Korea. The oysters were examined using the artificial digestion method followed by stereomicroscopy. The overall positive rate of G. seoi metacercariae in natural oysters was 66.0% (99/150), and the oysters collected from Yubu Island showed the highest infection rate (74.0%). However, the metacercarial density per oyster was relatively low (1.5-2.4 per oyster). By contrast, no metacercaria was found in cultured oysters purchased from 2 coastal areas in Chungcheongnam-do. Thus, we could confirm that natural oysters produced from 3 western coastal islands are infected with G. seoi metacercariae, whereas cultured oysters purchased from 2 coastal areas were free from infection.

• Parasites, Hosts and Diseases
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• 제36권2호
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• pp.133-142
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• 1998

참굴큰입흡충 (Gymnophalloides seoi)의 병원성을 규명하기 위한 연구의 일환으로 성충의 조효 소에서 단백분해효소를 분리 정제한 후 생화학적 특성을 관찰하였다. 조효소를 0.1 M sodium acetate (pH 4.5)로 투석한 후 원심분리하여 얻은 상층액을 Sephacris S-200 HR column chromatography로 부분 정제한 후 DEAE-Sephacelcolumnchromatography를 실시하여 순수 정 제하였다. SDS-PAGE를 실시하여 각 정제 단계별 시료의 정제도를 확인한 결과 분자량이 40 kDa 인 단일 분획이 관찰되었다. 정제된 효소는 시스테인 단백분해효소의 특이억제제인 L-lorans- epoxysuccinylleucylamido (4-guanidino) butane (E-64)와 iodoacetic acid, 세린 및 시스테인 단백분해효소의 일반 억제제인 leupeptin에 의해 활성이 억제되어 시스테인 단백분해효소임을 확 인하였다. 정제된 효소는 콜라겐, 파이브로넥틴과 같은 세포외 기질을 분해하였으나 헤모글로빈은 분해정도가 낮아 반응 12시간 후에도 단량체 (monomer)와 이합체 (dimer)의 양이 대조군과 큰 차이가 없었으며, IgGEa와 slgAE 거의 분해하지 믓하였다. 따라서, 참굴큰입흡충 성충의 40 kDa 시스테인 단백분해효소는 충체가 숙주 체내에서 기생생환을 하는데 필요한 영양분 섭취에 주로 관여할 것으로 생각되었다.

• Parasites, Hosts and Diseases
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• 제39권1호
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• pp.23-30
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• 2001

A nationwide survey was performed to know the distribution and prevalence of human Gymnophalloides seoi infection on western and southern coastal islands in the Republic of Korea. A total of 4,178 fecal specimens were collected Iron) residents on 45 (24 western and 21 southern) islands, and examined by Kato-Katz and formalin-ether sedimentation techniques. Eggs of G. seoi were detected from 160 (3.8%) people living on 22 (13 western and 9 southern) islands. The prevalence varied by the location of islands; higher on western islands than on southern islands . The highest prevalence was found on Amtaedo (25.3%), followed by Cheungdo (25.0%), and Anchwado (20.9%) (Shinan-gun). A little lower prevalence was observed on Munyodo (13.3%), Shinshido (12.9%), and Sonyudo (10.3%) (Kunsan-shi). Of the remaining islands, tile regions showing the prevalence greater than 5% included Kohado, Dallido (Mokpo-shi) . Pyeongildo, Kogumdo (Wando-gun) and Keogumdo (Kohung-gun). A strong age predilection was noted (p < 0.05): 95% of the infected people were over 40 years old. Females showed a little higher prevalence than males. The results indicate that human G. seoi infection is more widely distributed than previously considered. Nine of 11 islands (excluding the 2 known areas Munyodo and Sunyudo) that showed greater prevalence than 5% are regarded as new endemic foci of G. seoi.

• Parasites, Hosts and Diseases
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• 제51권5호
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• pp.589-594
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• 2013

In intestinal helminth infections, Th2 immune respones are generally associated with mucin secretion for worm expulsion from the host intestine. In particular, IL-4 and IL-13 are the important cytokines related with intestinal mucus production via STAT6 signalling in nematode infections. However, this perspective has never been studied in Gymnophalloides seoi infection. The present study aimed to observe the STAT6 signalling and cytokine responses in C57BL/6 mice, a mouse strain resistant to infection with this trematode. The results showed that worm expulsion occurred actively during days 1-2 post-infection (PI), when goblet cells began to proliferate in the small intestine. The STAT6 gene expression in the mouse spleen became remarkable from day 2 PI. Moreover, G. seoi infection induced a significant increase of IL-13 from day 4 PI in the spleen of infected mice. Our results suggested that goblet cell hyperplasia and worm expulsion in G. seoi-infected mice should be induced by STAT6 signalling, in which IL-13 may be involved as a dominant triggering cytokine.