• 한국식품저장유통학회지
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• 제13권4호
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• pp.501-505
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• 2006

진달래꽃 추출물의 항산화 효과와 인체 비인두암 세포인 KB cell에 대한 세포독성을 조사하였다. 또한 총 폴리페놀과 총 플라보노이드 함량을 측정하고 항산화성과의 상관성을 검토하였다. 진달래꽃 메탄올 추출물을 용매 분획하여 항산화 활성을 측정한 결과, 에칠아세테이트 분획의 활성이 가장 높게 나타났다. 에칠아세테이트 분획을 silica gel column chromatography로 부분 정제하여 항산화 실험을 한결과 fraction 2와 3은 40 ppm 농도에서 90% 이상의 활성을 나타내었다. 인체 비인두암 세포주인 KB cell의 성장 저해율은 $100{\mu}g/mL$ 농도에서 클로로포름 분획이 81.2%, 헥산분획이 74.6% 였다. 총 폴리페놀과 총 플라보노이드 함량을 측정한 결과, 에칠아세테이트 분획에는 총 폴리페놀 32.70%, 총 플라보노이드 20.30% 함유하고 있었다. 총 폴리페놀 및 총 플라보노이드 함량과 항산화성과의 상관관계는 총 폴리페놀에 대하여는 $r^2=0.8869$, 총 플라보노이드에 대하여는 $r^2=0.8469$로 상관성이 인정되었다.

• Journal of Dairy Science and Biotechnology
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• 제35권2호
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• pp.105-111
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• 2017

젖소, 한우, 돼지, 그리고 산양의 초유 중 단백질의 변화를 초일부터 7일까지 조사하였다. 돼지의 초유를 제외한 젖소, 한우, 그리고 산양의 초유에서 면역글로불린, 락토페린, 락토퍼옥시데이스, 혈청 알부민, IgG heavy chain, 그리고 IgG light chain은 분만 후, 초일 함량이 현저히 높았고, 2일째부터 급격히 감소하는 것을 보여 주었다. 그리고 ${\alpha}_{S2}$-카세인, ${\alpha}_{S1}$-카세인, ${\beta}$-카세인, ${\kappa}$-카세인, ${\beta}$-락토글로불린 및 ${\alpha}$-락트알부민은 분만 직후부터 7일까지 현저한 함량의 차이는 나타나지 않았다. 한편, 돼지 초유의 경우는 모든 단백질이 분만 후, 초일부터 2일까지 높은 함량을 나타내었다.

• 생태와환경
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• 제34권4호통권96호
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• pp.285-291
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• 2001

상위포식자에 의해 유도되는 Scenedesmus dimorphus (T$\ddot{u}$rpin) K$\ddot{u}$tzing의 군체형성에 관한 실험을 수행하였다. 동물플랑크톤 Daphnia magna (300 ind./L)와 Moina macrocopa (500 ind./L) 배양한후, $0.1\;{\mu}m$ (millipore)로 여과하여 얻어낸 용액 (ZFW)을 S. dimorphus가 1.8 ($10^5\;cells/ml$까지 자란 처리군에 첨가하여, ZFW를 넣지않은 대조군과 비교하였다. 대조군에 비해 두 동물플랑크톤 처리군에서 S. dimorphus의 군체형성의 유도가 뚜렷이 관찰되었다. 이 현상은 M. macrocopa 보다는 D. magna처리군에서 뚜렷하게 관찰되었으며, 첨가해준 ZFW의 양이 증가할수록 particle당 세포수도 비례적으로 증가하는 것으로 나타났다. ZFW처리군에서 1군체당 세포수(cells/colony)와 평균체적 (mean particle biovolume)은 $24{\sim}72$시간 사이에 급격히 증가하는 것으로 관찰되었다. S. dimorphus의 군체형성현상은 동물플랑크톤 D. magna와 M. macrocopa의 분비물에서 유도되는 화학물질의 영향으로 보이며, 이와 같은 형태변화현상은 동물플랑크톤의 포식에 대한 방어기작으로 작용하게 될 것으로 사료된다.

• 생약학회지
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• 제17권2호
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• pp.168-177
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• 1986

Bioassay-directed isolation has yielded some cytotoxic substances against L1210 cell from the Korean traditional medicine. These include 5,2'-dihydroxy-6,7,8,6'-teramethoxyflavone $(IV,\;scutellaria\;root,\;ED_{50}\;=\;1.7\;{mu}g/ml)$, 7-geranyloxycoumarin $(XXXII,\;poncirus\;fruit,\;10.2\;{mu}g/ml) $and panaxydol $(I,\;white\;ginseng,\;0.03\;{mu}g/ml)$. IV, XXXII and their derivatives were synthesized in the purpose of in vivo tests and for observation of structure-activity relations. Among the flavone derivatives, 5,2',6'-trihydroxy-6,7,8-trimethoxy flavone (XVIII), 5-hydroxy-6,7,8-trimethoxy-6'-benzyloxyflavone (XVII) and 5,8-dihydroxy-6,7-dimethoxyflavone (X) showed the cytotoxicity which has no correlation to the flavone structures. Of the coumarins synthesized, 7,8-dihydroxycoumarin (XXVI), 6-7-dihydroxycoumarin (XXIX) and 6-hydroxy-5,7-dimethoxycoumarin (XXXI) showed considerable activities. Acetylated XXXI has moderate activity $(ED_{50}=17.2\;{mu}g/ml)$. Monobydroxycoumarins or their methyl and allyl ether were inactive. IV inhibits the growth of the solid form of S-180 by 70% at 40 mg/kg and shows T/C of 166% on the ascitic S-180 at 40 mg/kg. It strongly inhibits the activity of the membrane bounded ATPase from L1210 cell. The most cytotoxic fraction of the antitumor materials studied is the one from the trichosanthes root showing $ED_{50}=0. 0003\;{mu}g/ml$ against L1210 cell. This fraction, obtained from ethyl acetate extract, showed T/C of 130 and 135%, on ICR mice bearing S-180 and $BDF_1$ mice bearing L1210 at 10 mg/kg and 7.5 mg/kg, respectively.

• The Plant Pathology Journal
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• 제21권1호
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• pp.68-72
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• 2005

The production of antibiotic substances by Serratia plymuthica A21-4 was greatly enhanced by modifying components of a growth medium. When the minimal medium containing $K_2HPO_4$ 0.7%, $KH_2PO_4$ 0.2%, $(NH_4)_2SO_4$ 0.1%, $MgSO_4$ 0.01% was used as basal medium, the best carbon source for antibiotic production was glycerol and the most favorable nitrogen source was ammonium sulfate. The modified medium for antibiotic production also increased colonization ability of A21-4 on pepper root and in the rhizosphere soil. When the cells of A21-4 were suspended in modified medium, the population density of A21-4 on pepper root was 10-100 times higher than that suspended in 0.1 M $MgSO_4$. The population density of A21-4 on root did not decrease under $10^6$ cfu/groot up to 21 days after treatment although the inoculum of A21-4 was reduced to $10^7$ cell/ml. Similar tendency was also observed in the rhizosphere soil. Consequently, Phytophthora blight of pepper was successfully controlled by A21-4 with $10^7$ cell/ml suspended in the modified buffer solution instead of $10^9$ cfu/ml suspended in 0.1 M $MgSO_4$.

• Parasites, Hosts and Diseases
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• 제53권1호
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• pp.21-27
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• 2015

Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were ${\alpha}$-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The $IC_{50}$ values for essential oil and methanolic extract was 8.4 and $28.9{\mu}g/ml$ against promastigotes, respectively. These values were 11.6 and $40.8{\mu}g/ml$ against amastigote forms, respectively. Glucantime as control drug also revealed $IC_{50}$ values of 88.3 and $44.6{\mu}g/ml$ for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

• 한국동물위생학회지
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• 제18권2호
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• pp.163-176
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• 1995

Triphenyl Tetrazolium Chloride(TCC) reduction test is simple and sensitive to some residual antibiotics (especially to penicillin) in milk, but comparatively insensible to sulfo-namides. The volumn of sample is also large. Thus this study was undertaken to increase the detectable level of sulfonamides in raw milk. In this study, we used small transparent plastic hole and pulp disc instead of 10m1 test tube and made test medium in which was added 0.08%TTC, 0.3% agar, 10% skim milk, approximately $10^6$ CFU/ml streptococcus thermophilus and 5ppm Trimethoprim to enhance the sensitivity for sulfonamides The results of TCC reduction test by disc plate method were summarized as follows : 1. sensitivity to residual sulfonamides were much higher than official TCC reduction test. Detectable limites of sulfamethazine, sulfamerazine, sulfathiazole, sulfachloropy-ridazine, sulfadimethoxine, sulfamononethoxine, sulfadiazine and sulfaquinoxaline were 0.1-0.5ppm levels. 2. Detectable limites to some antibiotics were simillar or good than that of official method as 0.005-0.1ppm to three ${\beta}$ -lactams, 0.25-0.5ppm to one macrolide, 2-10ppm to three aminoglycosides, 0.2-0.5ppm to three tetracycline, 0.1-0.5ppm to chloramphenicol. 3. Only 0.1ml of milk was needed to test and the test medium could be stored appnoximatly 7days in the refrigerator. So test procedure was convenient than offcial method. 4. These results suggest that disc plate method is more useful to detect bacterial growth inhibition substances including sulfonamides in raw milk.

• 환경위생공학
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• 제7권2호
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• pp.53-68
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• 1992

Since 1962, Korea has made a remarkable progress with continuous success of Five-Year Economic Development Plans. Over the last 30 years, Korea has achieved a fast growth in various industries including heavy industry and chemical industry with rapid urbanization at the same time. The fast industrialization and urbanization brought about huge amount of hazardous/toxic substances and wastes. The environmental pollution problems have now emerged from regional concerns to overall social issues accordingly. The situation has come to a point where pollution control and environment preservation have become one of the nation's major policies. Following the recent Earth Summit of UNCED Conference held in Riode Janeiro in June 1992, where the topic was environmentally sound and sustainable development, awareness upon the importance of environmental preservation has been heightened globally, The environmental issues will gradually influence the international society more and more, politically as well as economically, The environmental pollution control industry in Korea started in the early 70's with the development of the nation's industrialization, As the people's awareness on pollution increases and environmental laws and enforcing regulations were established, demand for pollution control began to increase. The environmental pollution control business came to be recognized as an independent industry in the mid'70's. It should be evaluated properly that over the last 30 years, the Korean pollution control industry has supplied locally manufactured pollution control facilities to the increasing local demand. Concerning the quality and customer satisfaction, however, there are still many aspects which need to be improved compared with those of advanced countries. Although the start of the pollution control industry in Korea is just 10 to 15 years behind the advanced countries such as Japan and European countries, current gap in the environmental technology is rather considerable. And, studies should be made to find out the reasons for the current technology gap, and therefore, to implement solutions to improve the technology and competitiveness of the environmental pollution control industry in Korea.

• 대한치과보철학회지
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• 제28권1호
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• pp.7-23
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• 1990

The success or failure of endosseous dental implants is related to the cellular activity at the implant surface. Success seems to be associated with the enclosure of the implant in a non-inflammed connective tissue or the formation of a direct bone implant interface. The purpose of this study was to examine the tissue reactions to the various implants at the submergible state in dog mandible. The $Br\"{a}nemark$, Core-Vent, Intergral, Bone spiral were selected for evaluation and also the Kimplant, Nephrite were used for the experimental study. After 4 months the animals were sacrificed. The interface zone between bone and implant was investigated using x-rays, light microscope, scanning electron microscope, transmission electron microscope. The following results were obtained from this study. 1. $Br\"{a}nemark$, Core-Vent, Kimplant, Integral showed no mobility and bone growth over the healing screws of the implants. Histologically most of the implant surface were covered by remodelled lamellar bone, and partly by a cellular layer or the thin fibrous tissue layer. 2. The Bone spiral showed no mobility and partially radiolucent line around the implant. The upper part of the implant was surrounded by a thin fibrous connective tissue and the middle, apical part of it were contacted with bone directly. 3. The Nephrite implant showed severe mobility and a radiolucent line around the implant. Histologically it showed mild inflammation and was surrounded by a fibrous connective tissue. 4. Scanning electron microscope showed that there was no amorphous ground substance in the Nephrite implant but the formation of ground substance over the collagen filaments in other implants. 5. Transmission electron microscope showed that collagen filaments were approached irregularly to the surface of all implants and in the $Br\"{a}nemark$, Core-Vent, Kimplant, Integral there was amorphous layer between the implant and the collagen filaments. It seemed to be ground substances.

• Journal of Periodontal and Implant Science
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• 제26권1호
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• pp.176-187
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• 1996

The ability of fibroblasts attached to teeth is paramount important in reestablishing the lost connective tissue attachment after periodontal therapy. The migration and proliferation of periodontal ligament cells are desired goal of periodontal regeneration therapy. PDGF is well known to regulate the cell activity of mesenchymal origin cell. Tobacco contains a complex mixture of substance including nicotine, various nitrosamines, trace elements, and variety of poorly characterized substances. Human gingival fibroblasts and periodontal ligament cells were cultured from extracted tooth for non-periodontal reason. Cultured human gingival fibroblasts and periodontal ligament cells in vitro were treated with PDGF, nicotine in time dependent manner. Cellular activities were determined by MTT assay. The purpose of this study was to determine the effects of Nicotine and PDGF, respectively and the effect of PDGF presence of nicotine on human gingival fibroblasts and periodontal ligament cells. The results were as follows : 1. In the cell activities of human gingival fibroblasts and periodontal ligament cells were similar or decreased to control value at 1st day. At 2nd day, cellular activities of both group were increased to control value. At 3rd day, cellular activities of both group were returned to the control value. 2. In the cell activities of PDGF on human gingival fibroblasts and periodontal ligament cells, cell activities significantly increase from control group on periodontal ligament cells compared to gingival fibroblast group at 3rd day. 3. In the cell activities of PDGF and nicotine combined application on human gingival fibroblasts and periodontal ligament cells, it seems likely that the nicotinic effect of gingival fibroblasts were higher than periodontal ligament cells and the PDGF effect of periodontal ligament cells were higher than gingival fibroblasts. This results suggested that PDGF might stimulate the selective growth on periodontal ligament cells.